Abstract:
:Using a combination of bioinformatic and molecular biology approaches a Drosophila melanogaster protein, DmTTF, has been identified, which exhibits sequence and structural similarity with two mitochondrial transcription termination factors, mTERF (human) and mtDBP (sea urchin). Import/processing assays indicate that DmTTF is synthesised as a precursor of 410 amino acids and is imported into mitochondria, giving rise to a mature product of 366 residues. Band-shift and DNase I protection experiments show that DmTTF binds two homologous, short, non-coding sequences of Drosophila mitochondrial DNA, located at the 3' end of blocks of genes transcribed on opposite strands. The location of the target sequences coincides with that of two of the putative transcription termination sites previously hypothesised. These results indicate that DmTTF is the termination factor of mitochondrial transcription in Drosophila. The existence of two DmTTF binding sites might serve not only to stop transcription but also to control the overlapping of a large number of transcripts generated by the peculiar transcription mechanism operating in this organism.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Roberti M,Polosa PL,Bruni F,Musicco C,Gadaleta MN,Cantatore Pdoi
10.1093/nar/gkg272keywords:
subject
Has Abstractpub_date
2003-03-15 00:00:00pages
1597-604issue
6eissn
0305-1048issn
1362-4962journal_volume
31pub_type
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