CACCC and GATA-1 sequences make the constitutively expressed alpha-globin gene erythroid-responsive in mouse erythroleukemia cells.


:Although the human alpha-globin and beta-globin genes are co-regulated in adult life, they achieve the same end by very different mechanisms. For example, a transfected beta-globin gene is expressed in an inducible manner in mouse erythroleukemia (MEL) cells while a transfected alpha-globin gene is constitutively expressed at a high level in induced and uninduced MEL cells. Interestingly, when the alpha-globin gene is transferred into MEL cells as part of human chromosome 16, it is appropriately expressed in an inducible manner. We explored the basis for the lack of erythroid-responsiveness of the proximal regulatory elements of the human alpha-globin gene. Since the alpha-globin gene is the only functional human globin gene that lacks CACCC and GATA-1 motifs, we asked whether their addition to the alpha-globin promoter would make the gene erythroid-responsive in MEL cells. The addition of each of these binding sites to the alpha-globin promoter separately did not result in inducibility in MEL cells. However, when both sites were added together, the alpha-globin gene became inducible in MEL cells. This suggests that erythroid non-responsiveness of the alpha-globin gene results from the lack of erythroid binding sites and is not necessarily a function of the constitutively active, GC rich promoter.


Nucleic Acids Res


Nucleic acids research


Ren S,Li J,Atweh GF




Has Abstract


1996-01-15 00:00:00














  • VCF.Filter: interactive prioritization of disease-linked genetic variants from sequencing data.

    abstract::Next generation sequencing is widely used to link genetic variants to diseases, and it has massively accelerated the diagnosis and characterization of rare genetic diseases. After initial bioinformatic data processing, the interactive analysis of genome, exome, and panel sequencing data typically starts from lists of ...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Müller H,Jimenez-Heredia R,Krolo A,Hirschmugl T,Dmytrus J,Boztug K,Bock C

    更新日期:2017-07-03 00:00:00

  • Characterization of the apolipoprotein B mRNA editing enzyme: no similarity to the proposed mechanism of RNA editing in kinetoplastid protozoa.

    abstract::Intestinal apolipoprotein B mRNA is edited at nucleotide 6666 by a C to U transition resulting in a translational stop codon. The enzymatic properties of the editing activity were characterised in vitro using rat enterocyte cytosolic extract. The editing activity has no nucleotide or ion cofactor requirement. It shows...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Greeve J,Navaratnam N,Scott J

    更新日期:1991-07-11 00:00:00

  • NGS-based identification and tracing of microsatellite instability from minute amounts DNA using inter-Alu-PCR.

    abstract::Sensitive detection of microsatellite instability (MSI) in tissue or liquid biopsies using next generation sequencing (NGS) has growing prognostic and predictive applications in cancer. However, the complexities of NGS make it cumbersome as compared to established multiplex-PCR detection of MSI. We present a new appro...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Yu F,Leong KW,Makrigiorgos A,Adalsteinsson VA,Ladas I,Ng K,Mamon H,Makrigiorgos GM

    更新日期:2020-12-08 00:00:00

  • Efficient detection of RNA-protein interactions using tethered RNAs.

    abstract::The diverse localization of transcripts in cells suggests that there are many specific RNA-protein interactions that have yet to be identified. Progress has been limited, however, by the lack of a robust method to detect and isolate the RNA-binding proteins. Here we describe the use of an RNA aptamer, scaffolded to a ...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Iioka H,Loiselle D,Haystead TA,Macara IG

    更新日期:2011-04-01 00:00:00

  • Spectral clustering of protein sequences.

    abstract::An important problem in genomics is automatically clustering homologous proteins when only sequence information is available. Most methods for clustering proteins are local, and are based on simply thresholding a measure related to sequence distance. We first show how locality limits the performance of such methods by...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Paccanaro A,Casbon JA,Saqi MA

    更新日期:2006-03-17 00:00:00

  • G-quadruplex recognition and remodeling by the FANCJ helicase.

    abstract::Guanine rich nucleic acid sequences can form G-quadruplex (G4) structures that interfere with DNA replication, repair and RNA transcription. The human FANCJ helicase contributes to maintaining genomic integrity by promoting DNA replication through G4-forming DNA regions. Here, we combined single-molecule and ensemble ...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Wu CG,Spies M

    更新日期:2016-10-14 00:00:00

  • A homogeneous nucleic acid hybridization assay based on strand displacement.

    abstract::A homogeneous nucleic acid hybridization assay which is conducted in solution and requires no separation steps is described. The assay is based on the concept of strand displacement. In the strand displacement assay, an RNA "signal strand" is hybridized within a larger DNA strand termed the "probe strand", which is, i...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Vary CP

    更新日期:1987-09-11 00:00:00

  • RCAS: an RNA centric annotation system for transcriptome-wide regions of interest.

    abstract::In the field of RNA, the technologies for studying the transcriptome have created a tremendous potential for deciphering the puzzles of the RNA biology. Along with the excitement, the unprecedented volume of RNA related omics data is creating great challenges in bioinformatics analyses. Here, we present the RNA Centri...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Uyar B,Yusuf D,Wurmus R,Rajewsky N,Ohler U,Akalin A

    更新日期:2017-06-02 00:00:00

  • Alteration of the DNA double helix conformation upon incorporation of mispairs as revealed by energy computations and pathways of point mutations.

    abstract::To explain biochemical and genetic data on spontaneous nucleotide replacements in nucleic acid biosynthesis all the 8 mispairs in normal tautomeric forms have been considered. Possible B-conformations of DNA fragments containing each of such mispairs incorporated between Watson-Crick pairs have been found using comput...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Chuprina VP,Poltev VI

    更新日期:1985-01-11 00:00:00

  • Cloning of two novel forms of human acidic fibroblast growth factor (aFGF) mRNA.

    abstract::We have previously isolated two different aFGF cDNA clones from kidney and brain. The two corresponding mRNA, designated aFGF 1.A and 1.B, are the predominant species in kidney and brain, respectively. During the characterization of aFGF mRNA in glioblastoma cells, we demonstrated that aFGF mRNA in U1242MG and D65MG g...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Payson RA,Canatan H,Chotani MA,Wang WP,Harris SE,Myers RL,Chiu IM

    更新日期:1993-02-11 00:00:00

  • Identification of putative transcriptional regulatory networks in Entamoeba histolytica using Bayesian inference.

    abstract::Few transcriptional regulatory networks have been described in non-model organisms. In Entamoeba histolytica seminal aspects of pathogenesis are transcriptionally controlled, however, little is known about transcriptional regulatory networks that effect gene expression in this parasite. We used expression data from tw...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Hackney JA,Ehrenkaufer GM,Singh U

    更新日期:2007-01-01 00:00:00

  • Splicing of constitutive upstream introns is essential for the recognition of intra-exonic suboptimal splice sites in the thrombopoietin gene.

    abstract::The human thrombopoietin (TPO) gene, which codes for the principal cytokine involved in platelet maturation, shows a peculiar alternative splicing of its last exon, where an intra-exonic 116 nt alternative intron is spliced out in a fraction of its mRNA. To characterize the molecular mechanism underlying this alternat...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Romano M,Marcucci R,Baralle FE

    更新日期:2001-02-15 00:00:00

  • Unusual ribosome binding properties of mRNA encoding bacteriophage lambda repressor.

    abstract::The mRNA encoding repressor cI of phage lambda is the only known E. coli message which starts directly with the initiation AUG codon. The ability of in vitro synthesized cI mRNA fragments (150 or 400 nts) to form ternary initiation complexes has been studied using the toeprint method. In the presence of tRNA(Met)f, th...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Balakin AG,Skripkin EA,Shatsky IN,Bogdanov AA

    更新日期:1992-02-11 00:00:00

  • HOMCOS: a server to predict interacting protein pairs and interacting sites by homology modeling of complex structures.

    abstract::As protein-protein interactions are crucial in most biological processes, it is valuable to understand how and where protein pairs interact. We developed a web server HOMCOS (Homology Modeling of Complex Structure, to predict interacting protein pairs and interacting sites by homology mo...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Fukuhara N,Kawabata T

    更新日期:2008-07-01 00:00:00

  • Nucleotide sequences of the retroviral long terminal repeats and their adjacent regions.

    abstract::The nucleotide sequences of the LTRs and their adjacent regions from 19 type C and one type B retrovirus were compared. Salient features are: (a) The R regions in the genomes of most of the type C retroviruses begin with GC and end with CA. (b) The mammalian type C retroviruses have a polyadenylation signal "AATAAA" i...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Chen HR,Barker WC

    更新日期:1984-02-24 00:00:00

  • Epigenetic features of human telomeres.

    abstract::Although subtelomeric regions in humans are heterochromatic, the epigenetic nature of human telomeres remains controversial. This controversy might have been influenced by the confounding effect of subtelomeric regions and interstitial telomeric sequences (ITSs) on telomeric chromatin structure analyses. In addition, ...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Cubiles MD,Barroso S,Vaquero-Sedas MI,Enguix A,Aguilera A,Vega-Palas MA

    更新日期:2018-03-16 00:00:00

  • Processing of complementary sense RNAs of Digitaria streak virus in its host and in transgenic tobacco.

    abstract::We have used a polymerase chain reaction (PCR) procedure to analyse low abundance complementary sense RNAs of Digitaria streak virus (DSV) from infected leaves of Digitaria setigera. This study has confirmed that both spliced and unspliced RNAs are synthesised by the same transcription unit. The position of the intron...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Mullineaux PM,Guerineau F,Accotto GP

    更新日期:1990-12-25 00:00:00

  • On the cooperative and noncooperative binding of ethidium to DNA.

    abstract::The equilibrium binding of ethidium bromide to native DNAs and to poly(dG-dC) X poly(dG-dC) has been studied by both phase partition and direct spectrophotometric techniques. The binding isotherms obtained from both experimental techniques show that ethidium binds in a cooperative manner to E. coli DNA. On the other h...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Winkle SA,Rosenberg LS,Krugh TR

    更新日期:1982-12-20 00:00:00

  • Efficient silencing of gene expression with modular trimeric Pol II expression cassettes comprising microRNA shuttles.

    abstract::Expressed polycistronic microRNA (miR) cassettes have useful properties that can be utilized for RNA interference (RNAi)-based gene silencing. To advance their application we generated modular trimeric anti-hepatitis B virus (HBV) Pol II cassettes encoding primary (pri)-miR-31-derived shuttles that target three differ...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Ely A,Naidoo T,Arbuthnot P

    更新日期:2009-07-01 00:00:00

  • ChIPprimersDB: a public repository of verified qPCR primers for chromatin immunoprecipitation (ChIP).

    abstract::Chromatin immunoprecipitation (ChIP) has ushered in a new era of scientific discovery by allowing new insights into DNA-protein interactions. ChIP is used to quantify enriched genomic regions using qPCR, and more recently is combined with next generation sequencing (ChIP-seq) to obtain a genome wide profile of protein...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Kurtenbach S,Reddy R,Harbour JW

    更新日期:2019-01-08 00:00:00

  • The nucleotide sequence of 5.8S rRNA from the posterior silk gland of the silkworm Philosamia cynthia ricini.

    abstract::The nucleotide sequence of 5.8S rRNA from the Chinese silkworm Philosamia cynthia ricini has been determined by gel sequencing and mobility shift methods. The complete primary structure is (sequence in text). This is one of the largest known 5.8S rRNAs. As compared to Bombyx 5.8S rRNA, it is two nucleotides longer; t...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Feng YX,Krupp G,Gross HJ

    更新日期:1982-10-25 00:00:00

  • An oxidized nucleotide affects DNA replication through activation of protein kinases in Xenopus egg lysates.

    abstract::To elucidate the response to oxidative stress in eukaryotic cells, the effect of an oxidized nucleotide, 8-oxo-2'-deoxyguanosine 5'-triphosphate (8-oxo-dGTP), generated from dGTP with an active oxygen, on DNA synthesis was studied using a cell-free DNA replication system derived from Xenopus egg lysates with a single-...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Kai T,Matsunaga R,Eguchi M,Kamiya H,Kasai H,Suzuki M,Izuta S

    更新日期:2002-01-15 00:00:00

  • The high fidelity and unique error signature of human DNA polymerase epsilon.

    abstract::Bulk replicative DNA synthesis in eukaryotes is highly accurate and efficient, primarily because of two DNA polymerases (Pols): Pols δ and ε. The high fidelity of these enzymes is due to their intrinsic base selectivity and proofreading exonuclease activity which, when coupled with post-replication mismatch repair, he...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Korona DA,Lecompte KG,Pursell ZF

    更新日期:2011-03-01 00:00:00

  • Repeated DNA sequences in fungi.

    abstract::Several fungal species, representatives of all broad groups like basidiomycetes, ascomycetes and phycomycetes, were examined for the nature of repeated DNA sequences by DNA:DNA reassociation studies using hydroxyapatite chromatography. All of the fungal species tested contained 10-20% repeated DNA sequences. There are...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Dutta SK

    更新日期:1974-11-01 00:00:00

  • KEGG for integration and interpretation of large-scale molecular data sets.

    abstract::Kyoto Encyclopedia of Genes and Genomes (KEGG, or is a database resource that integrates genomic, chemical and systemic functional information. In particular, gene catalogs from completely sequenced genomes are linked to higher-level systemic functions of the cell, the o...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Kanehisa M,Goto S,Sato Y,Furumichi M,Tanabe M

    更新日期:2012-01-01 00:00:00

  • Masking repeats while clustering ESTs.

    abstract::A problem in EST clustering is the presence of repeat sequences. To avoid false matches, repeats have to be masked. This can be a time-consuming process, and it depends on available repeat libraries. We present a fast and effective method that aims to eliminate the problems repeats cause in the process of clustering. ...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Schneeberger K,Malde K,Coward E,Jonassen I

    更新日期:2005-04-14 00:00:00

  • Sequential stimulation of cellular RNA synthesis in polyoma-infected mouse kidney cell cultures.

    abstract::Lytic infection with polyoma virus leads in Go-arrested primary mouse kidney cell cultures to a mitotic host response. In the present work we focused our attention on cellular RNA synthesis shortly after onset of polyoma T-antigen synthesis. Onset of polyoma-induced stimulation of 45S pre-rRNA synthesis was determined...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Matter JM,Tiercy JM,Weil R

    更新日期:1983-10-11 00:00:00

  • Effects of double-strand break repair proteins on vertebrate telomere structure.

    abstract::Although telomeres are not recognized as double-strand breaks (DSBs), some DSB repair proteins are present at telomeres and are required for telomere maintenance. To learn more about the telomeric function of proteins from the homologous recombination (HR) and non-homologous end joining pathways (NHEJ), we have screen...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Wei C,Skopp R,Takata M,Takeda S,Price CM

    更新日期:2002-07-01 00:00:00

  • Further biochemical characterization of wheat DNA primase: possible functional implication of copurification with DNA polymerase A.

    abstract::DNA primase has been partially purified from wheat germ. This enzyme, like DNA primases characterized from many procaryotic and eucaryotic sources, catalyses the synthesis of primers involved in DNA replication. However, the wheat enzyme differs from animal DNA primase in that it is found partially associated with a D...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Laquel P,Castroviejo M,Litvak S

    更新日期:1990-08-25 00:00:00

  • High resolution crystal structure of domain I of the Saccharomyces cerevisiae homing endonuclease PI-SceI.

    abstract::The homing endonuclease PI-SceI from Saccharo myces cerevisiae consists of two domains. The protein splicing domain I catalyzes the excision of the mature endonuclease (intein) from a precursor protein and the religation of the flanking amino acid sequences (exteins) to a functional protein. Furthermore, domain I is i...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Werner E,Wende W,Pingoud A,Heinemann U

    更新日期:2002-09-15 00:00:00