Abstract:
:Liquid-liquid phase separation plays an important role in a variety of cellular processes, including the formation of membrane-less organelles, the cytoskeleton, signalling complexes, and many other biological supramolecular assemblies. Studies on the molecular basis of phase separation in cells have focused on protein-driven phase separation. In contrast, there is limited understanding on how RNA specifically contributes to phase separation. Here, we described a phase-separation-like phenomenon that SHORT ROOT (SHR) RNA undergoes in cells. We found that an RNA G-quadruplex (GQ) forms in SHR mRNA and is capable of triggering RNA phase separation under physiological conditions, suggesting that GQs might be responsible for the formation of the SHR phase-separation-like phenomenon in vivo. We also found the extent of GQ-triggered-phase-separation increases on exposure to conditions which promote GQ. Furthermore, GQs with more G-quartets and longer loops are more likely to form phase separation. Our studies provide the first evidence that RNA can adopt structural motifs to trigger and/or maintain the specificity of RNA-driven phase separation.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Zhang Y,Yang M,Duncan S,Yang X,Abdelhamid MAS,Huang L,Zhang H,Benfey PN,Waller ZAE,Ding Ydoi
10.1093/nar/gkz978subject
Has Abstractpub_date
2019-12-16 00:00:00pages
11746-11754issue
22eissn
0305-1048issn
1362-4962pii
5624975journal_volume
47pub_type
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