Abstract:
:3'-O-(5'-phosphoryldeoxycytidyl) phosphorothioate and fluorescent 3'-O-(5'-phosphoryldeoxycytidyl) S-bimane phosphorothioate can be ligated to tRNA by T4 RNA ligase. They are also efficient donors for the enzymatic ligation to oligodeoxynucleotides bearing a 3'-cytidine terminus. Cytidine 3',5'-bisphosphate is also a substrate for the ligation reaction with DNA restriction fragments with a 3'-terminate cytidylic acid residue. Oligo- and polynucleotides with a 3'-phosphorothioate group react readily with electrophiles as exemplified by the reaction with monobromobimane.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Cosstick R,McLaughlin LW,Eckstein Fdoi
10.1093/nar/12.4.1791subject
Has Abstractpub_date
1984-02-24 00:00:00pages
1791-810issue
4eissn
0305-1048issn
1362-4962journal_volume
12pub_type
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