Multi-forms of human MTH1 polypeptides produced by alternative translation initiation and single nucleotide polymorphism.

abstract：:The nucleotide sequence of Drosophila melanogaster methionine tRNAi was determined to be: pA-G-C-A-G-A-G-U-m1G-m2G-C-G-C-A-G-U-G-G-A-A-G-C-G-U-m2G-C-U-G-G-G-C-C-C-A-U-t6A-A-C-C-C-A-G-A-G-m7G-D-m5C-C-C-G-A-G-G-A-U-C-G-m1A-A-A-C-C-U-U-G-C-U-C-U-G-C-U-A-C-C-A(OH). It differs from vertebrate initiator tRNAs in only 6 out ...

journal_title：Nucleic acids research

authors： Silverman S,Heckman J,Cowling GJ,Delaney AD,Dunn RJ,Gillam IC,Tener GM,Söll D,RajBhandary UL

更新日期：1979-02-01 00:00:00

abstract：:CAG repeats occur predominantly in the coding regions of human genes, which suggests their functional importance. In some genes, these sequences can undergo pathogenic expansions leading to neurodegenerative polyglutamine (poly-Q) diseases. The mutant transcripts containing expanded CAG repeats possibly contribute to ...

journal_title：Nucleic acids research

authors： Kiliszek A,Kierzek R,Krzyzosiak WJ,Rypniewski W

更新日期：2010-12-01 00:00:00

abstract：:Molecular replacement (MR) is the method of choice for X-ray crystallography structure determination when structural homologues are available in the Protein Data Bank (PDB). Although the success rate of MR decreases sharply when the sequence similarity between template and target proteins drops below 35% identical res...

journal_title：Nucleic acids research

authors： Claude JB,Suhre K,Notredame C,Claverie JM,Abergel C

更新日期：2004-07-01 00:00:00

abstract：:The remarkable selectivity of N-methyl mesoporphyrin IX (NMM) for G-quadruplexes (GQs) is long known, however its ability to stabilize and bind GQs has not been investigated in detail. Through the use of circular dichroism, UV-visible spectroscopy and fluorescence resonance energy transfer (FRET) melting assay we have...

journal_title：Nucleic acids research

authors： Nicoludis JM,Barrett SP,Mergny JL,Yatsunyk LA

更新日期：2012-07-01 00:00:00

abstract：:Specific guanine-rich regions in human genome can form higher-order DNA structures called G-quadruplexes, which regulate many relevant biological processes. For instance, the formation of G-quadruplex at telomeres can alter cellular functions, inducing apoptosis. Thus, developing small molecules that are able to bind ...

journal_title：Nucleic acids research

authors： Di Leva FS,Novellino E,Cavalli A,Parrinello M,Limongelli V

更新日期：2014-05-01 00:00:00

abstract：:In this study we describe a novel method to investigate the RNA-RNA interactions between a small RNA and its target that we termed 'RNA walk'. The method is based on UV-induced AMT cross-linking in vivo followed by affinity selection of the hybrid molecules and mapping the intermolecular adducts by RT-PCR or real-time...

journal_title：Nucleic acids research

authors： Lustig Y,Wachtel C,Safro M,Liu L,Michaeli S

更新日期：2010-01-01 00:00:00

abstract：:Ribosomal DNA (rDNA) from Xenopus laevis contains several heterogeneities in all three transcribed spacers, as revealed by analysis of cloned and uncloned amplified rDNA from oocytes and cloned chromosomal rDNA from erythrocytes. Heterogeneities include single base changes and length variants of one to several nucleot...

journal_title：Nucleic acids research

authors： Stewart MA,Hall LM,Maden BE

更新日期：1983-02-11 00:00:00

abstract：:OGG1 initiated base excision repair (BER) is the major pathway for repair of oxidative DNA base damage 8-oxoguanine (8-oxoG). Here, we report that RECQL4 DNA helicase, deficient in the cancer-prone and premature aging Rothmund-Thomson syndrome, physically and functionally interacts with OGG1. RECQL4 promotes catalytic...

journal_title：Nucleic acids research

authors： Duan S,Han X,Akbari M,Croteau DL,Rasmussen LJ,Bohr VA

更新日期：2020-07-09 00:00:00

abstract：:DNA curvature plays a well-characterized role in many transcriptional regulation mechanisms. We present evidence for the conservation of curvature signals in putative regulatory regions of several archaeal and eubacterial genomes. Genes with highly curved upstream regions were identified in orthologous groups, based o...

journal_title：Nucleic acids research

authors： Jáuregui R,Abreu-Goodger C,Moreno-Hagelsieb G,Collado-Vides J,Merino E

更新日期：2003-12-01 00:00:00

abstract：:Plasmid encoded replication initiation (Rep) proteins recruit host helicases to plasmid replication origins. Previously, we showed that RepD recruits directionally the PcrA helicase to the pC221 oriD, remains associated with it, and increases its processivity during plasmid unwinding. Here we show that RepD forms a co...

journal_title：Nucleic acids research

authors： Machón C,Lynch GP,Thomson NH,Scott DJ,Thomas CD,Soultanas P

更新日期：2010-04-01 00:00:00

abstract：:A library of three synthetic ribozymes with randomized arms, targeting NUX, GUX and NXG triplets, respectively, were used to identify ribozyme-accessible sites on the HIV-1 LTR transcript comprising positions -533 to 386. Three cleavable sites were identified at positions 109, 115 and 161. Ribozymes were designed agai...

journal_title：Nucleic acids research

authors： Bramlage B,Luzi E,Eckstein F

更新日期：2000-11-01 00:00:00

abstract：:This year's Database Issue of Nucleic Acids Research contains 152 papers that include descriptions of 54 new databases and update papers on 98 databases, of which 16 have not been previously featured in NAR As always, these databases cover a broad range of molecular biology subjects, including genome structure, gene e...

journal_title：Nucleic acids research

authors： Galperin MY,Fernández-Suárez XM,Rigden DJ

更新日期：2017-01-04 00:00:00

abstract：:Treatment of responsive cells by interferons (IFNs) induces within a few hours a rise in the concentration of several proteins and mRNAs. In order to characterize these IFN-induced mRNA species, we have cloned in E. coli the cDNA made from a 17-18S poly(A)+ RNA of human fibroblastoid cells (SV80) treated with IFN-beta...

journal_title：Nucleic acids research

authors： Chebath J,Merlin G,Metz R,Benech P,Revel M

更新日期：1983-03-11 00:00:00

abstract：:Muscle creatine kinase (MCK) is a major enzyme of cellular energy metabolism that is expressed upon differentiation of myoblasts into myotubes. Previously we cloned and sequenced the entire rabbit enzyme cDNA which was used as a probe in these studies to obtain a genomic clone from a rabbit library. The transcription ...

journal_title：Nucleic acids research

authors： Yi TM,Walsh K,Schimmel P

更新日期：1991-06-11 00:00:00

abstract：:The advent of jellyfish green fluorescent protein and its spectral variants, together with promising new fluorescent proteins from other classes of the Cnidarian phylum (coral and anemones), has greatly enhanced and promises to further boost the detection and localization of proteins in cell biology. It has been less ...

journal_title：Nucleic acids research

authors： Pederson T

更新日期：2001-03-01 00:00:00

abstract：:The results of differential expression analyses provide scientists with hundreds to thousands of differentially expressed genes that need to be interpreted in light of the biology of the specific system under study. This requires mapping the genes to functional classifications that can be, for example, the KEGG pathwa...

journal_title：Nucleic acids research

authors： D'Andrea D,Grassi L,Mazzapioda M,Tramontano A

更新日期：2013-07-01 00:00:00

abstract：:Small interfering RNAs (siRNAs) are now established as the preferred tool to inhibit gene function in mammalian cells yet trigger unintended gene silencing due to their inherent miRNA-like behavior. Such off-target effects are primarily mediated by the sequence-specific interaction between the siRNA seed regions (posi...

journal_title：Nucleic acids research

authors： Bramsen JB,Pakula MM,Hansen TB,Bus C,Langkjær N,Odadzic D,Smicius R,Wengel SL,Chattopadhyaya J,Engels JW,Herdewijn P,Wengel J,Kjems J

更新日期：2010-09-01 00:00:00

abstract：:Exposure to genotoxic agents, such as ionizing radiation (IR), produces double-strand breaks, repaired predominantly in mammalian cells by non-homologous end-joining (NHEJ). Ku70 was identified as an interacting partner of a proteolytic Cyclin E (CycE) fragment, p18CycE. p18CycE endogenous generation during IR-induced...

journal_title：Nucleic acids research

authors： Chatterjee P,Plesca D,Mazumder S,Boutros J,Yannone SM,Almasan A

更新日期：2013-12-01 00:00:00

abstract：:The process of strand exchange is considered to be the hallmark of DNA recombination. Proteins known to carry out such exchange are believed to operate via one or the other of two mechanisms. RecA-like proteins promote the formation of a three-stranded or triplex synaptic intermediate in which strand exchange occurs, ...

journal_title：Nucleic acids research

authors： Jean D,Gendron D,Delbecchi L,Bourgaux P

更新日期：1997-10-15 00:00:00

abstract：:Analysis of intergenic sequences for purposes such as the investigation of transcriptional signals or the identification of small RNA genes is frequently complicated by traditional biological database structures. Genome data is commonly treated as chromosome-length sequence records, detailed by gene calls demarcating ...

journal_title：Nucleic acids research

authors： Ray WC,Daniels CJ

更新日期：2003-01-01 00:00:00

abstract：:Decay of (125)I produces a shower of low energy electrons (Auger electrons) that cause strand breaks in DNA in a distance-dependent manner with 90% of the breaks located within 10 bp from the decay site. We studied strand breaks in RNA molecules produced by decay of (125)I incorporated into complementary DNA oligonucl...

journal_title：Nucleic acids research

authors： Gaidamakova EK,Neumann RD,Panyutin IG

更新日期：2002-11-15 00:00:00

abstract：:A mass spectral method is described for the detection and identification of unusual nucleotide residues present in DNAs. Analysis by this method of intact, underivatized DNA from salmon sperm, calf thymus, mouse L-cells, wheat germ, M. lysodeikticus, E. Coli, and the bacteriophages 0X-174, fd, and lamda, yields diagno...

journal_title：Nucleic acids research

authors： Wiebers JL

更新日期：1976-11-01 00:00:00

abstract：:Inability of T7 RNA polymerase to processively transcribe higher eukaryotic chromatin is interpreted as a correlate of its reported inhibition by nucleosomes on reconstituted templates in vitro . We used chromosomally integrated reporter cassettes to examine features of T7 transcription in a lower eukaryotic system. L...

journal_title：Nucleic acids research

authors： Wirtz E,Hoek M,Cross GA

更新日期：1998-10-15 00:00:00

abstract：:The S-adenosylmethionine (AdoMet) analog sinefungin is a natural product antibiotic that inhibits nucleic acid methyltransferases and arrests the growth of unicellular eukarya and eukaryal viruses. The basis for the particular sensitivity of fungi and protozoa to sinefungin is not known. Here we report the isolation a...

journal_title：Nucleic acids research

authors： Zheng S,Shuman S,Schwer B

更新日期：2007-01-01 00:00:00

abstract：:Recruitment of RAD18 to stalled replication forks facilitates monoubiquitination of PCNA during S-phase, promoting translesion synthesis at sites of UV irradiation-induced DNA damage. In this study, we show that RAD18 is also recruited to ionizing radiation (IR)-induced sites of DNA double-strand breaks (DSBs) forming...

journal_title：Nucleic acids research

authors： Watanabe K,Iwabuchi K,Sun J,Tsuji Y,Tani T,Tokunaga K,Date T,Hashimoto M,Yamaizumi M,Tateishi S

更新日期：2009-04-01 00:00:00

abstract：:Human somatic cells have essentially no telomerase activity. Telomerase is linked to tumor genesis and is a valuable marker for malignant growth. Extreme paucity of the enzyme neccessitated development of a PCR-based assay, 'telomeric repeat amplification protocol' (TRAP). Unfortunately, this method is not without dif...

journal_title：Nucleic acids research

authors： Krupp G,Kühne K,Tamm S,Klapper W,Heidorn K,Rott A,Parwaresch R

更新日期：1997-02-15 00:00:00

abstract：:A complete physical map of the spinach mitochondrial genome has been established. The entire sequence content of 327 kilobase pairs (kb) is postulated to occur as a single circular molecule. Two directly repeated elements of approximately 6 kb, located on this "master chromosome", are proposed to participate in an int...

journal_title：Nucleic acids research

authors： Stern DB,Palmer JD

更新日期：1986-07-25 00:00:00

abstract：:PIR-International is an association of macromolecular sequence data collection centers dedicated to fostering international cooperation as an essential element in the development of scientific databases. A major objective of PIR-International is to continue the development of the Protein Sequence Database as an essent...

journal_title：Nucleic acids research

authors： George DG,Barker WC,Mewes HW,Pfeiffer F,Tsugita A

更新日期：1994-09-01 00:00:00

abstract：:(19)F NMR spectroscopy has proved to be a valuable tool to monitor functionally important conformational transitions of nucleic acids. Here, we present a systematic investigation on the application of 5-fluoro pyrimidines to probe DNA and RNA secondary structures. Oligonucleotides with the propensity to adapt secondar...

journal_title：Nucleic acids research

authors： Puffer B,Kreutz C,Rieder U,Ebert MO,Konrat R,Micura R

更新日期：2009-12-01 00:00:00

abstract：:I show that the recognition sequences of Type II restriction systems are correlated with the G + C content of the host bacterial DNA. Almost all restriction systems with G + C rich tetranucleotide recognition sequences are found in species with A + T rich genomes, whereas G + C rich hexanucleotide and octanucleotide r...

journal_title：Nucleic acids research

authors： McClelland M

更新日期：1988-03-25 00:00:00