Conditional DNA repair mutants enable highly precise genome engineering.

abstract：:MiRNAs post-transcriptionally regulate gene expression by recruiting the miRNA-induced silencing complex (miRISC) to target mRNAs. However, the mechanisms by which miRISC components are maintained at appropriate levels for proper function are largely unknown. Here, we demonstrate that Caenorhabditis elegans TEG-1 regu...

journal_title：Nucleic acids research

authors： Wang C,Gupta P,Fressigne L,Bossé GD,Wang X,Simard MJ,Hansen D

更新日期：2017-02-17 00:00:00

abstract：:About 40% of the genes in the nematode Caenorhabditis elegans have homologs in humans. Based on the history of this model system, it is clear that the application of genetic methods to the study of this set of genes would provide important clues to their function in humans. To facilitate such genetic studies, we are e...

journal_title：Nucleic acids research

authors： Edgley M,D'Souza A,Moulder G,McKay S,Shen B,Gilchrist E,Moerman D,Barstead R

更新日期：2002-06-15 00:00:00

abstract：:LRPPRC is a protein that has attracted interest both for its role in post-transcriptional regulation of mitochondrial gene expression and more recently because numerous mutated variants have been characterized as causing severe infantile mitochondrial neurodegeneration. LRPPRC belongs to the pentatricopeptide repeat (...

journal_title：Nucleic acids research

authors： Spåhr H,Rozanska A,Li X,Atanassov I,Lightowlers RN,Chrzanowska-Lightowlers ZM,Rackham O,Larsson NG

更新日期：2016-08-19 00:00:00

abstract：:Fluorescence-based sequencing is playing an increasingly important role in efforts to identify DNA polymorphisms and mutations of biological and medical interest. The application of this technology in generating the reference sequence of simple and complex genomes is also driving the development of new computer progra...

journal_title：Nucleic acids research

authors： Nickerson DA,Tobe VO,Taylor SL

更新日期：1997-07-15 00:00:00

abstract：:Mitochondrial DNA is a sensitive target of chemical carcinogens (Backer and Weinstein (1980) Science 209, 297-299), suggesting that mutations of the mitochondrial genome occur in tumor cells. We examined this point by comparing mitochondrial DNA sequences in four rat tumors with those of normal rat liver. Some novel m...

journal_title：Nucleic acids research

authors： Taira M,Yoshida E,Kobayashi M,Yaginuma K,Koike K

更新日期：1983-03-25 00:00:00

abstract：:In the known monoclinic crystals the 3-dimensional structure of the hexameric, replicative helicase RepA encoded by plasmid RSF1010 shows 6-fold rotational symmetry. In contrast, in the cubic crystal form at 2.55 A resolution described here RepA has 3-fold symmetry and consists of a trimer of dimers. To study structur...

journal_title：Nucleic acids research

authors： Ziegelin G,Niedenzu T,Lurz R,Saenger W,Lanka E

更新日期：2003-10-15 00:00:00

abstract：:Here we employ a set of RNA Polymerase II (Pol II) activity mutants to determine the consequences of increased or decreased Pol II catalysis on gene expression in Saccharomyces cerevisiae. We find that alteration of Pol II catalytic rate, either fast or slow, leads to decreased Pol II occupancy and apparent reduction ...

journal_title：Nucleic acids research

authors： Malik I,Qiu C,Snavely T,Kaplan CD

更新日期：2017-05-05 00:00:00

abstract：:Codon usage data has been compiled for 110 yeast genes. Cluster analysis on relative synonymous codon usage revealed two distinct groups of genes. One group corresponds to highly expressed genes, and has much more extreme synonymous codon preference. The pattern of codon usage observed is consistent with that expected...

journal_title：Nucleic acids research

authors： Sharp PM,Tuohy TM,Mosurski KR

更新日期：1986-07-11 00:00:00

abstract：:Previous evidence indicates that telomeres resemble common fragile sites and present a challenge for DNA replication. The precise impediments to replication fork progression at telomeric TTAGGG repeats are unknown, but are proposed to include G-quadruplexes (G4) on the G-rich strand. Here we examined DNA synthesis and...

journal_title：Nucleic acids research

authors： Lormand JD,Buncher N,Murphy CT,Kaur P,Lee MY,Burgers P,Wang H,Kunkel TA,Opresko PL

更新日期：2013-12-01 00:00:00

abstract：:The finding in animal species of complexes homologous to the products of six Saccharomyces cerevisiae genes, origin of replication recognition complex (ORC), has suggested that ORC-related mechanisms have been conserved in all eukaryotes. In plants, however, the only cloned putative homologs of ORC subunits are the Ar...

journal_title：Nucleic acids research

authors： Witmer X,Alvarez-Venegas R,San-Miguel P,Danilevskaya O,Avramova Z

更新日期：2003-01-15 00:00:00

abstract：:More and more evidences demonstrate that the long non-coding RNAs (lncRNAs) play many key roles in diverse biological processes. There is a critical need to annotate the functions of increasing available lncRNAs. In this article, we try to apply a global network-based strategy to tackle this issue for the first time. ...

journal_title：Nucleic acids research

authors： Guo X,Gao L,Liao Q,Xiao H,Ma X,Yang X,Luo H,Zhao G,Bu D,Jiao F,Shao Q,Chen R,Zhao Y

更新日期：2013-01-01 00:00:00

abstract：:In Xenopus oocytes in vitro transcribed mouse U7 RNA is assembled into small nuclear ribonucleoproteins (snRNPs) that are functional in histone RNA 3' processing. If the special Sm binding site of U7 (AAUUUGUCUAG, U7 Sm WT) is converted into the canonical Sm sequence derived from the major snRNAs (AAUUUUUGGAG, U7 Sm O...

journal_title：Nucleic acids research

authors： Stefanovic B,Hackl W,Lührmann R,Schümperli D

更新日期：1995-08-25 00:00:00

abstract：:VL30 genetic elements constitute a murine multicopy gene family that is retrovirus-like, despite the lack of sequence homology with any known retrovirus. Over one hundred copies of VL30 units are dispersed throughout the mouse genome. We report here that the mouse genome also contains 'solo' VL30 long terminal repeats...

journal_title：Nucleic acids research

authors： Rotman G,Itin A,Keshet E

更新日期：1984-03-12 00:00:00

abstract：:Chicken-erythrocyte inner histone tetramer has been complexed with several natural and synthetic DNA duplexes by salt-gradient dialysis at various protein/DNA ratios. The resulting complexes, in low-ionic-strength buffer, have been examined by electron microscopy, circular dichroism, and thermal denaturation. Electron...

journal_title：Nucleic acids research

authors： Bryan PN,Wright EB,Hsie MH,Olins AL,Olins DE

更新日期：1978-10-01 00:00:00

abstract：:In eukaryotes, RNA-binding proteins that contain multiple K homology (KH) domains play a key role in coordinating the different steps of RNA synthesis, metabolism and localization. Understanding how the different KH modules participate in the recognition of the RNA targets is necessary to dissect the way these protein...

journal_title：Nucleic acids research

authors： Hollingworth D,Candel AM,Nicastro G,Martin SR,Briata P,Gherzi R,Ramos A

更新日期：2012-08-01 00:00:00

abstract：:Expressed Sequence Tag-based gene expression profiling can be used to discover functionally associated genes on a large scale. Currently available web servers and tools focus on finding differentially expressed genes in different samples or tissues rather than finding co-expressed genes. To fill this gap, we have deve...

journal_title：Nucleic acids research

authors： Wu X,Walker MG,Luo J,Wei L

更新日期：2005-07-01 00:00:00

abstract：:The Mouse Genome Database (MGD) (http://www.informatics.jax.org) is the community model organism database resource for the laboratory mouse, a premier animal model for the study of genetic and genomic systems relevant to human biology and disease. MGD maintains a comprehensive catalog of genes, functional RNAs and oth...

journal_title：Nucleic acids research

authors： Blake JA,Bult CJ,Eppig JT,Kadin JA,Richardson JE,Mouse Genome Database Group.

更新日期：2014-01-01 00:00:00

abstract：:A novel method to measure mRNA levels has been developed by combining the detection capabilities of RNase protection (RPA) with the quantification advantages of scintillation proximity assay (SPA) technology. Sample processing is reduced to the addition of a single reagent post RNase digestion. As a model system, the ...

journal_title：Nucleic acids research

authors： Kenrick MK,Jiang L,Potts CL,Owen PJ,Shuey DJ,Econome JG,Anson JG,Quinet EM

更新日期：1997-07-15 00:00:00

abstract：:Chromatin footprinting in Drosophila tissue culture cells has detected the binding of a non-histone protein at +8 of the distal Adh RNA start site, on a 10-bp direct repeat motif abutting a nucleosome positioned over the inactive Adh distal promoter. Alternatively the active promoter is bound by a transcription initia...

journal_title：Nucleic acids research

authors： Benyajati C,Ewel A,McKeon J,Chovav M,Juan E

更新日期：1992-09-11 00:00:00

abstract：:Abasic sites are common DNA lesions resulting from spontaneous depurination and excision of damaged nucleobases by DNA repair enzymes. However, the influence of the local sequence context on the structure of the abasic site and ultimately, its recognition and repair, remains elusive. In the present study, duplex DNAs ...

journal_title：Nucleic acids research

authors： Chen J,Dupradeau FY,Case DA,Turner CJ,Stubbe J

更新日期：2008-01-01 00:00:00

abstract：:Oligodeoxyribonucleotides incorporating non-hydrolyzable dialkyl sulfide linked thymidine dimers (TsT) were synthesized chemically by the solid-phase approach. The sulfide dimer TsT was stable to degradation by snake-venom phosphodiesterase, calf spleen phosphodiesterase, Nuclease P1 and Nuclease S1. Thermal denaturat...

journal_title：Nucleic acids research

authors： Kawai SH,Wang D,Giannaris PA,Damha MJ,Just G

更新日期：1993-03-25 00:00:00

abstract：:PPARγ2 is a critical lineage-determining transcription factor that is essential for adipogenic differentiation. Here we report characterization of the three-dimensional structure of the PPARγ2 locus after the onset of adipogenic differentiation and the mechanisms by which it forms. We identified a differentiation-depe...

journal_title：Nucleic acids research

authors： LeBlanc SE,Wu Q,Lamba P,Sif S,Imbalzano AN

更新日期：2016-06-20 00:00:00

abstract：:The human lymphoid cell lines Walker and Daudi constitute a particularly suitable system for studies on the chromatin structure of K light chain genes (see preceding paper). The rearranged and non-rearranged alleles of Walker cells were found to be about equally sensitive towards digestion with DNAase I. A DNAase I hy...

journal_title：Nucleic acids research

authors： Pospelov VA,Klobeck HG,Zachau HG

更新日期：1984-09-25 00:00:00

abstract：:The complete DNA sequence of pO157, the large virulence plasmid of EHEC strain O157:H7 EDL 933, is presented. The 92 kb F-like plasmid is composed of segments of putative virulence genes in a framework of replication and maintenance regions, with seven insertion sequence elements, located mostly at the boundaries of t...

journal_title：Nucleic acids research

authors： Burland V,Shao Y,Perna NT,Plunkett G,Sofia HJ,Blattner FR

更新日期：1998-09-15 00:00:00

abstract：:The RDM1 gene encodes a RNA recognition motif (RRM)-containing protein involved in the cellular response to the anti-cancer drug cisplatin in vertebrates. We previously reported a cDNA encoding the full-length human RDM1 protein. Here, we describe the identification of 11 human cDNAs encoding RDM1 protein isoforms. Th...

journal_title：Nucleic acids research

authors： Messaoudi L,Yang YG,Kinomura A,Stavreva DA,Yan G,Bortolin-Cavaillé ML,Arakawa H,Buerstedde JM,Hainaut P,Cavaillé J,Takata M,Van Dyck E

更新日期：2007-01-01 00:00:00

abstract：:In kinetoplastid protozoa, mitochondrial (mt) mRNAs are post-transcriptionally edited by insertion and deletion of uridylate residues, the information being provided by guide (g)RNAs. Currently popular mechanisms for the editing process envisage a series of consecutive 'cut-and-paste' reactions, carried out by a compl...

journal_title：Nucleic acids research

authors： Blom D,Burg Jv,Breek CK,Speijer D,Muijsers AO,Benne R

更新日期：2001-07-15 00:00:00

abstract：:Members of the HMG-I(Y) family of mammalian nonhistone proteins are of importance because they have been demonstrated to bind specifically to the minor groove of A.T-rich sequences both in vitro and in vivo and to function as gene transcriptional regulatory proteins in vivo. Here we report the cloning, sequencing, cha...

journal_title：Nucleic acids research

authors： Friedmann M,Holth LT,Zoghbi HY,Reeves R

更新日期：1993-09-11 00:00:00

abstract：:We have analyzed the DNaseI sensitivity of chromatin from the rat albumin and alpha-fetoprotein genes in the fetal liver (which synthesizes albumin and alpha-fetoprotein), adult liver (which synthesizes albumin), fetal yolk sac (which synthesizes alpha-fetoprotein), and adult kidney (which synthesizes neither). Active...

journal_title：Nucleic acids research

authors： Kunnath L,Locker J

更新日期：1985-01-11 00:00:00

abstract：:The Bovine Genome Database (BGD; http://BovineGenome.org) strives to improve annotation of the bovine genome and to integrate the genome sequence with other genomics data. BGD includes GBrowse genome browsers, the Apollo Annotation Editor, a quantitative trait loci (QTL) viewer, BLAST databases and gene pages. Genome ...

journal_title：Nucleic acids research

authors： Childers CP,Reese JT,Sundaram JP,Vile DC,Dickens CM,Childs KL,Salih H,Bennett AK,Hagen DE,Adelson DL,Elsik CG

更新日期：2011-01-01 00:00:00

abstract：:Evidence has emerged that repair of clustered DNA lesions may be compromised, possibly leading to the formation of double-strand breaks (DSB) and, thus, to deleterious events. The first repair event occurring at a multiply damaged site (MDS) is of major importance and will largely contribute to the hazardousness of MD...

journal_title：Nucleic acids research

authors： Eot-Houllier G,Gonera M,Gasparutto D,Giustranti C,Sage E

更新日期：2007-01-01 00:00:00