Abstract:
:Oligodeoxyribonucleotides incorporating non-hydrolyzable dialkyl sulfide linked thymidine dimers (TsT) were synthesized chemically by the solid-phase approach. The sulfide dimer TsT was stable to degradation by snake-venom phosphodiesterase, calf spleen phosphodiesterase, Nuclease P1 and Nuclease S1. Thermal denaturation analysis indicated that the incorporation of TsT dimers into DNA weakened, but did not prevent, binding to complementary DNA and RNA over a wide range of salt concentrations (10 mM to 2 M NaCl).
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Kawai SH,Wang D,Giannaris PA,Damha MJ,Just Gdoi
10.1093/nar/21.6.1473subject
Has Abstractpub_date
1993-03-25 00:00:00pages
1473-9issue
6eissn
0305-1048issn
1362-4962journal_volume
21pub_type
杂志文章abstract::Cytosines in genomic DNA are sometimes methylated. This affects many biological processes and diseases. The standard way of measuring methylation is to use bisulfite, which converts unmethylated cytosines to thymines, then sequence the DNA and compare it to a reference genome sequence. We describe a method for the cri...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks275
更新日期:2012-07-01 00:00:00
abstract::BioModels Database (http://www.ebi.ac.uk/biomodels/), part of the international initiative BioModels.net, provides access to published, peer-reviewed, quantitative models of biochemical and cellular systems. Each model is carefully curated to verify that it corresponds to the reference publication and gives the proper...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkj092
更新日期:2006-01-01 00:00:00
abstract::Spliced leader (SL) trans-splicing is a critical element of gene expression in a number of eukaryotic groups. This process is arguably best understood in nematodes, where biochemical and molecular studies in Caenorhabditis elegans and Ascaris suum have identified key steps and factors involved. Despite this, the preci...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx500
更新日期:2017-08-21 00:00:00
abstract::Synthesis of (p)ppRNA-DNA chains by purified HeLa cell DNA primase-DNA polymerase alpha (pol alpha-primase) was compared with those synthesized by a multiprotein form of DNA polymerase alpha (pol alpha 2) using unique single-stranded DNA templates containing the origin of replication for simian virus 40 (SV40) DNA. Th...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/14.18.7305
更新日期:1986-09-25 00:00:00
abstract::In this article, we introduce the Gypsy Database (GyDB) of mobile genetic elements, an in-progress database devoted to the non-redundant analysis and evolutionary-based classification of mobile genetic elements. In this first version, we contemplate eukaryotic Ty3/Gypsy and Retroviridae long terminal repeats (LTR) ret...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm697
更新日期:2008-01-01 00:00:00
abstract::IslandViewer (http://pathogenomics.sfu.ca/islandviewer) is a web-accessible application for the computational prediction and analysis of genomic islands (GIs) in bacterial and archaeal genomes. GIs are clusters of genes of probable horizontal origin and are of high interest because they disproportionately encode virul...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt394
更新日期:2013-07-01 00:00:00
abstract::Many multimeric transcription factors recognize DNA sequence patterns by cooperatively binding to bipartite elements composed of half sites separated by a flexible spacer. We developed a novel bipartite algorithm, bipartite pattern discovery (Bipad), which produces a mathematical model based on information maximizatio...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkh825
更新日期:2004-09-23 00:00:00
abstract::Analysis of intergenic sequences for purposes such as the investigation of transcriptional signals or the identification of small RNA genes is frequently complicated by traditional biological database structures. Genome data is commonly treated as chromosome-length sequence records, detailed by gene calls demarcating ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg013
更新日期:2003-01-01 00:00:00
abstract::Au (III) reacts with DNA at pH 5.6 to form a complex which is sensitive to mid-UV radiation. Cyclobutane pyrimidine dimers are produced at some 15 to 30 times the rate that they are in untreated DNA. The mechanism of photosensitization appears to involve energy absorption by Au-urine and Au-cytosine adducts which can ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/5.10.3731
更新日期:1978-10-01 00:00:00
abstract::A simple and rapid procedure to isolate clones carrying sequences from a specific region of the polytene chromosome of Drosophila is demonstrated. The procedure involves microdissection of the region of interest, amplification of the DNA by PCR using a primer designed to prime the synthesis nonspecifically, labeling o...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/18.3.599
更新日期:1990-02-11 00:00:00
abstract::Restriction endonucleases HindII and TaqI, but not SalI, were found to efficiently cleave synthetic hexadecanucleotide duplexes which contained either an A/C or a G/T mismatch within their respective restriction sites. Double-stranded M13 DNAs with identical mismatches were also cleaved under the assay conditions. The...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/14.5.1943
更新日期:1986-03-11 00:00:00
abstract::A study of the synthesis of oligothymidylates via phosphotriester intermediates on a polystyrene support is described. The sequence involves condensation of a phenyl nucleoside-3' -phosphorochloridate with the 5'-hydroxyl group of the carrier bound oligonucleotide derivative in the presence of 1-methylimidazole. Condi...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/2.6.773
更新日期:1975-06-01 00:00:00
abstract::While antibiotic selection has been routinely used for the selection of genetically modified cells, administration of cytotoxic drugs often leads to deleterious effects not only to inert cells but also to transfected or transduced ones. In this study, we propose an Antigen-MEdiated Genetically modified cell Amplificat...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gng032
更新日期:2003-04-01 00:00:00
abstract::Here we analyze the effect of DNA folding on the performance of short primers and describe a simple technique for assessing hitherto uncertain values of thermodynamic parameters that determine the folding of single-stranded DNA into secondary structure. An 8mer with two degenerate positions is extended simultaneously ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/26.23.5525
更新日期:1998-12-01 00:00:00
abstract::Selenoproteins contain the amino acid selenocysteine which is encoded by a UGA Sec codon. Recoding UGA Sec requires a complex mechanism, comprising the cis-acting SECIS RNA hairpin in the 3'UTR of selenoprotein mRNAs, and trans-acting factors. Among these, the SECIS Binding Protein 2 (SBP2) is central to the mechanism...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp078
更新日期:2009-04-01 00:00:00
abstract::Reactive oxygen species produced by endogenous metabolic activity and exposure to a multitude of exogenous agents impact cells in a variety of ways. The DNA base damage 8-oxodeoxyguanosine (8-oxodG) is a prominent indicator of oxidative stress and has been well-characterized as a premutagenic lesion in mammalian cells...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/27.15.3213
更新日期:1999-08-01 00:00:00
abstract::Insertion of the insertion sequence Is2(I) directly before the galE gene of the galactose operon results in a Gal minus phenotype (1, 2). The Gal-constitutive allele galc200 (and its deletion derivative galc200 delta 31) arise from such a Gal minus mutant by the insertion of LS2(II) DNA within the LS2(I) sequence (3)....
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/10.16.5015
更新日期:1982-08-25 00:00:00
abstract::The RNA Editing Database (REDIdb) is an interactive, web-based database created and designed with the aim to allocate RNA editing events such as substitutions, insertions and deletions occurring in a wide range of organisms. The database contains both fully and partially sequenced DNA molecules for which editing infor...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl793
更新日期:2007-01-01 00:00:00
abstract::In many viruses and transposons, expression of some genes requires alternative reading of the genetic code, also called recoding. Such events depend on specific mRNA sequences and can lead to read through of an in-frame stop codon or to +1 or -1 frameshifting. Here, we addressed the issue of conservation of recoding r...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/23.9.1557
更新日期:1995-05-11 00:00:00
abstract::DNA labeled for 15 minutes during UV induced repair synthesis is two-fold more sensitive to micrococcal nuclease than the bulk nuclear DNA. As the length of the labeling period increases from 15 minutes to 4 hours the nuclease sensitivity of repair labeled DNA approaches that of bulk chromatin. Pulse-chase experiments...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/9.1.203
更新日期:1981-01-10 00:00:00
abstract::Friedreich ataxia (FRDA) is caused by hyperexpansion of GAA*TTC repeats located in the first intron of the FXN gene, which inhibits transcription leading to the deficiency of frataxin. The FXN gene is an excellent target for therapeutic intervention since (i) 98% of patients carry the same type of mutation, (ii) the m...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkn604
更新日期:2008-11-01 00:00:00
abstract::A set of new sulfurizing agents representing disulfides of arylsulfonic acids has been developed for the automated synthesis of phosphorothioate oligonucleotide analogues via the phosphoramidite method. These reagents, such as bis(benzenesulfonyl)disulfide, bis(p-toluenesulfonyl)disulfide, bis(p-methoxybenzensulfonyl)...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/23.20.4029
更新日期:1995-10-25 00:00:00
abstract::In the presence of S-adenosyl methionine the 5' terminal guanosine residue of in vitro synthesized reovirus mRNA becomes methylated at the 2'-OH position. In addition, 7-methyl guanylic acid is condensed covalently at the 5' terminus resulting in the formation of a 5' to 5' triphosphate bridge. Analysis of the 5' term...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/2.8.1329
更新日期:1975-08-01 00:00:00
abstract::Sequence-specific interactions between aminoacyl-tRNA synthetases and their cognate tRNAs ensure both accurate RNA recognition and the efficient catalysis of aminoacylation. The effects of tRNA(Trp)variants on the aminoacylation reaction catalyzed by wild-type Escherichia coli tryptophanyl-tRNA synthe-tase (TrpRS) hav...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/27.18.3631
更新日期:1999-09-15 00:00:00
abstract::Giant chromatin-modifying complexes regulate gene transcription in eukaryotes by acting on chromatin substrates and 'setting' the histone code. The histone deacetylase (HDAC)-associated mammalian Sin3 corepressor complex regulates a wide variety of genes involved in all aspects of cellular physiology. The recruitment ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp051
更新日期:2009-04-01 00:00:00
abstract::Robust methods to detect DNA-binding proteins from structures of unknown function are important for structural biology. This paper describes a method for identifying such proteins that (i) have a solvent accessible structural motif necessary for DNA-binding and (ii) a positive electrostatic potential in the region of ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkh803
更新日期:2004-09-08 00:00:00
abstract::We show that nucleic acid structures may be conveniently and inexpensively characterized by their UV thermal difference spectra. A thermal difference spectrum (TDS) is obtained for a nucleic acid by simply recording the ultraviolet absorbance spectra of the unfolded and folded states at temperatures above and below it...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gni134
更新日期:2005-09-12 00:00:00
abstract::In mammalian cells, DNA double-strand breaks (DSBs) are repaired by three pathways, nonhomologous end-joining (NHEJ), gene conversion (GC) and single-strand annealing (SSA). These pathways are distinct with regard to repair efficiency and mutagenic potential and must be tightly controlled to preserve viability and gen...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkn347
更新日期:2008-07-01 00:00:00
abstract::Sgs1 is a member of the RecQ family of DNA helicases, which have been implicated in genomic stability, cancer and ageing. Srs2 is another DNA helicase that shares several phenotypic features with Sgs1 and double sgs1srs2 mutants have a severe synthetic growth phenotype. This suggests that there may be functional overl...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/30.5.1103
更新日期:2002-03-01 00:00:00
abstract::Enzymatic hydrolysis and base analysis by high performance liquid chromatography showed that mouse satellite DNA had 30-50% less 5-methylcytosine in sperm than in somatic tissue (1.59 mols % vs 2.40-3.11 mols %). Maxam-Gilbert sequencing and analysis of the intensity of the cytosine bands indicated that the level of m...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/13.11.3969
更新日期:1985-06-11 00:00:00