Abstract:
:A simple and rapid procedure to isolate clones carrying sequences from a specific region of the polytene chromosome of Drosophila is demonstrated. The procedure involves microdissection of the region of interest, amplification of the DNA by PCR using a primer designed to prime the synthesis nonspecifically, labeling of the amplified DNA using the random primer method, and screening of a standard library with the probe to identify and isolate clones carrying sequences homologous to the dissected region. This procedure has the potential to replace the difficult procedure of microcloning, as well as facilitate chromosome walking.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Wesley CS,Ben M,Kreitman M,Hagag N,Eanes WFdoi
10.1093/nar/18.3.599subject
Has Abstractpub_date
1990-02-11 00:00:00pages
599-603issue
3eissn
0305-1048issn
1362-4962journal_volume
18pub_type
杂志文章abstract::Scanning tunneling microscopy (STM) reveals nanometer scale details of hydrated DNA but the interpretation of the images is controversial because of substrate artifacts and the lack of a theory for image contrast. We demonstrate that we have overcome these problems by identifying five DNA samples by their STM images a...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/21.25.5896
更新日期:1993-12-25 00:00:00
abstract::RNase III enzymes cleave double stranded (ds)RNA. This is an essential step for regulating the processing of mRNA, rRNA, snoRNA and other small RNAs, including siRNA and miRNA. Arabidopsis thaliana encodes nine RNase III: four DICER-LIKE (DCL) and five RNASE THREE LIKE (RTL). To better understand the molecular functio...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx820
更新日期:2017-11-16 00:00:00
abstract::The Dnd (DNA degradation) phenotype, reflecting a novel DNA modification by sulfur in Streptomyces lividans 1326, was strongly aggravated when one (dndB) of the five genes (dndABCDE) controlling it was mutated. Electrophoretic banding patterns of a plasmid (pHZ209), reflecting DNA degradation, displayed a clear change...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm176
更新日期:2007-01-01 00:00:00
abstract::A number of meiosis-specific transcripts are selectively eliminated during the mitotic cell cycle in fission yeast. Mmi1, an RNA-binding protein, plays a crucial role in this selective elimination. Mmi1 recognizes a specific region, namely, the determinant of selective removal (DSR) on meiotic transcripts and induces ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt763
更新日期:2013-11-01 00:00:00
abstract::The interplay between aromatic stacking and hydrogen bonding in nucleobases has been investigated via high-level quantum chemical calculations. The experimentally observed stacking arrangement between consecutive bases in DNA and RNA/DNA double helices is shown to enhance their hydrogen bonding ability as opposed to g...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gki317
更新日期:2005-03-23 00:00:00
abstract::The 5' processing of rat pre-tRNA(Lys) and a series of mutant derivatives by rat cytosolic RNase P was examined. In standard, non-kinetic assays, mutant precursors synthesized in vitro with 5' leader sequences of 10, 17, 24, 25, and 46 nucleotides were processed to approximately equal levels and yielded precisely clea...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/22.16.3347
更新日期:1994-08-25 00:00:00
abstract::A new method is reported for the synthesis of oligodeoxyribonucleotides containing 2-aminopurine residues at selected sites. This method involves protection of the 2-aminopurine ribonucleoside, reduction to the deoxyribonucleoside and standard preparation of the 5'-0- (4,4'-dimethoxytrityl)-3'-O-(2-cyanoethyl)-N,N- di...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/24.4.754
更新日期:1996-02-15 00:00:00
abstract::8-Oxo-7,8-dihydroguanine (8-oxoGua) is produced in cells by reactive oxygen species normally formed during cellular metabolic processes. This oxidized base can pair with both adenine and cytosine, and thus the existence of this base in messenger RNA would cause translational errors. The MutT protein of Escherichia col...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gki682
更新日期:2005-07-07 00:00:00
abstract::NMR methods were used to investigate a series of mutants of the pseudoknot within the gene 32 messenger RNA of bacteriophage T2, for the purpose of investigating the range of sequences, stem and loop lengths that can form a similar pseudoknot structure. This information is of particular relevance since the T2 pseudokn...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/25.6.1130
更新日期:1997-03-15 00:00:00
abstract::To explain biochemical and genetic data on spontaneous nucleotide replacements in nucleic acid biosynthesis all the 8 mispairs in normal tautomeric forms have been considered. Possible B-conformations of DNA fragments containing each of such mispairs incorporated between Watson-Crick pairs have been found using comput...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/13.1.141
更新日期:1985-01-11 00:00:00
abstract::The Escherichia coli AlkB protein catalyzes the direct reversal of alkylation damage to DNA; primarily 1-methyladenine (1mA) and 3-methylcytosine (3mC) lesions created by endogenous or environmental alkylating agents. AlkB is a member of the non-heme iron (II) alpha-ketoglutarate-dependent dioxygenase superfamily, whi...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm1031
更新日期:2007-01-01 00:00:00
abstract::Cisplatin analogues were synthesised that consisted of platinum(II) diamine complexes tethered via a polymethylene chain ( n = 3, 5, 8 and 10) to a phenanthridinium cation. Both chloro and iodo leaving groups were examined. DNA adduct formation was quantitatively analysed using a linear amplification system with the p...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/26.17.3933
更新日期:1998-09-01 00:00:00
abstract::The nucleoside compositions of chloroplast and cytoplasmic tRNAPhe's from Euglena gracilis have been determined. The modified nucleoside compositions of these two tRNAs indicate that tRNAPheChl is more similar to procaryotic (E. coli) tRNAPhe than to either the Euglena cytoplasmic tRNAPhe or other eucaryotic cytoplasm...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/3.2.371
更新日期:1976-02-01 00:00:00
abstract::The human beta-actin promoter, including its 5' flanking region and 5' untranslated region, is ubiquitously active in mammalian cells in culture. In this report we investigated the transcriptional activity of, and the protein-DNA interactions that occur within, the proximal region of the human beta-actin promoter. Eff...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/19.24.6913
更新日期:1991-12-25 00:00:00
abstract::Restriction fragments containing established curved regions, such as the pBR322 tn3 region, the phage lambda attP region and the SV40 T-antigen and terminus of replication regions, exhibit systematically retarded elution upon anion exchange based HPLC. Using this method, we were able to detect readily other SV40 curve...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/19.15.4181
更新日期:1991-08-11 00:00:00
abstract::Double-stranded (ds) oligodeoxynucleotides (29mers) containing an O6-ethylguanine (O6-EtGua) flanked 5' and 3' by different bases (5'..TGT..3'; 5'..CGG..3', 5'..GGT..3'; 5'..GGG..3'; 5'..GGA..3') were synthesized to investigate the binding and repair characteristics of recombinant human O6-alkylguanine-DNA alkyltransf...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/24.11.2087
更新日期:1996-06-01 00:00:00
abstract::DNA double stranded breaks (DSBs) are one of the most deleterious types of DNA lesions. The main pathways responsible for repairing these breaks in eukaryotic cells are homologous recombination (HR) and non-homologous end-joining (NHEJ). However, a third group of still poorly characterized DSB repair pathways, collect...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv1349
更新日期:2016-04-07 00:00:00
abstract::Comparative genome hybridization (CGH) to DNA microarrays (array CGH) is a technique capable of detecting deletions and duplications in genomes at high resolution. However, array CGH studies of the human genome noting false negative and false positive results using large insert clones as probes have raised important c...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gki643
更新日期:2005-06-16 00:00:00
abstract::DIAL (dihedral alignment) is a web server that provides public access to a new dynamic programming algorithm for pairwise 3D structural alignment of RNA. DIAL achieves quadratic time by performing an alignment that accounts for (i) pseudo-dihedral and/or dihedral angle similarity, (ii) nucleotide sequence similarity a...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm334
更新日期:2007-07-01 00:00:00
abstract::Plasmids containing a dihydrofolate reductase (DHFR) expression unit were transfected into DHFR-deficient Chinese hamster ovary (CHO) cells. Methotrexate exposure was used to select cells with amplified DHFR sequences. Three cell lines were isolated containing amplified copies of transfected DNA that had integrated in...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/17.4.1697
更新日期:1989-02-25 00:00:00
abstract::U4 RNA from chicken, rat and man was examined for nucleotide sequence and secondary structure. Three molecular species, U4A, U4B and U4C were detected in the three animal species. U4A is 146 nucleotide long and U4B RNA only lacks the 3' terminal G. four nucleotides are missing at the 3'-end of U4C RNA which, in additi...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/9.12.2699
更新日期:1981-06-25 00:00:00
abstract::The oncogenic transformation of normal cells into malignant, rapidly proliferating cells requires major alterations in cell physiology. For example, the transformed cells remodel their metabolic processes to supply the additional demand for cellular building blocks. We have recently demonstrated essential metabolic pr...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv1337
更新日期:2016-01-04 00:00:00
abstract::Oligodeoxyribonucleotides containing N4-methoxycytosine (mo4C), N4-methoxy-5-methylcytosine (mo4m5C) and other base-analogues were synthesised and used to compare the stabilities of duplexes containing mo4C.A and mo4C.G base pairs with those containing normal and mismatch pairs. The Tm values and other thermodynamic p...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/15.20.8167
更新日期:1987-10-26 00:00:00
abstract::Regulation of gene transcription in both prokaryotes and eukaryotes involves formation of various DNA-multiprotein complexes of higher order structure through communication between distant regions of DNA. The communication between distant DNA sites occurs by interaction between proteins bound to the sites by looping o...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/25.4.873
更新日期:1997-02-15 00:00:00
abstract::Multiple regulatory modules contribute to the complex expression programs realized by many loci. Although long thought of as isolated components, recent studies demonstrate that such regulatory sequences can physically associate with promoters and with each other and may localize to specific sub-nuclear transcription ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq1160
更新日期:2011-04-01 00:00:00
abstract::We demonstrate compaction of DNA with nanoscale biomimetic constructs which are robust synthetic analogs of globular proteins. These constructs are approximately 15 nm in diameter, shell crosslinked knedel-like (SCKs) nanoparticles, which are prepared by covalent stabilization of amphiphilic di-block co-polymer micell...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/27.14.2966
更新日期:1999-07-15 00:00:00
abstract::Using total internal reflection fluorescence microscopy, we directly visualize in real-time, the 1D Brownian motion and transcription elongation of T7 RNA polymerase along aligned DNA molecules bound to substrates by molecular combing. We fluorescently label T7 RNA polymerase with antibodies and use flow to convect th...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm332
更新日期:2007-01-01 00:00:00
abstract::The complete nucleotide sequence of the coding region of foot and mouth disease virus RNA (strain A1061) is presented. The sequence extends from the primary initiation site, approximately 1200 nucleotide from the 5' end of the genome, in an open translational reading frame of 6,999 nucleotides to a termination codon 9...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/12.5.2461
更新日期:1984-03-12 00:00:00
abstract::Plant orthologs of the yeast sucrose non-fermenting (Snf1) kinase and mammalian AMP-activated protein kinase (AMPK) represent an emerging class of important regulators of metabolic and stress signalling. The catalytic alpha-subunits of plant Snf1-related kinases (SnRKs) interact in the yeast two-hybrid system with dif...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/29.17.3685
更新日期:2001-09-01 00:00:00
abstract::Fourteen P-element insertion mutants at the yellow (y) gene of D. melanogaster have been analysed by restriction mapping and DNA sequencing. The distribution of the insertion sites is nonrandom, with a preferred target site in the 5'-transcribed but non-translated leader of the gene. Transcription analysis indicates t...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/16.7.3039
更新日期:1988-04-11 00:00:00