Identification of DNA--cisplatin adducts in a blind trial of in situ scanning tunneling microscopy.

abstract：:The complete nucleotide sequence of the Escherichia coli recB gene which encodes a subunit of the ATP-dependent DNase, Exonuclease V, has been determined. The proposed coding region for the RecB protein is 3543 nucleotides long and would encode a polypeptide of 1180 amino acids with a calculated molecular weight of 13...

journal_title：Nucleic acids research

authors： Finch PW,Storey A,Chapman KE,Brown K,Hickson ID,Emmerson PT

更新日期：1986-11-11 00:00:00

abstract：:Two analog uridine triphosphates tethering additional functionality, one a primary amino group and the second a mercapto group, were prepared and tested for their compatibility with in vitro RNA selection procedures. 5-(3-Aminopropyl)uridine triphosphate (UNH(2)) as a uridine substitute was a more effective substrate ...

journal_title：Nucleic acids research

authors： Vaish NK,Fraley AW,Szostak JW,McLaughlin LW

更新日期：2000-09-01 00:00:00

abstract：:Deamination of cytosine (C), 5-methylcytosine (mC) and 5-hydroxymethylcytosine (hmC) occurs spontaneously in mammalian DNA with several hundred deaminations occurring in each cell every day. The resulting potentially mutagenic mispairs of uracil (U), thymine (T) or 5-hydroxymethyluracil (hmU) with guanine (G) are subs...

journal_title：Nucleic acids research

authors： Kemmerich K,Dingler FA,Rada C,Neuberger MS

更新日期：2012-07-01 00:00:00

abstract：:A growing body of evidence suggests that genes transcribed by RNA polymerase III exhibit multiple functions within a chromosome. While the predominant function of these genes is the synthesis of RNA molecules, certain RNA polymerase III genes also function as genomic landmarks. Transfer RNA genes are known to exhibit ...

journal_title：Nucleic acids research

authors： Simms TA,Miller EC,Buisson NP,Jambunathan N,Donze D

更新日期：2004-09-30 00:00:00

abstract：:Optical DNA mapping (ODM) allows visualization of long-range sequence information along single DNA molecules. The data can for example be used for detecting long range structural variations, for aiding DNA sequence assembly of complex genomes and for mapping epigenetic marks and DNA damage across the genome. ODM tradi...

journal_title：Nucleic acids research

authors： Müller V,Dvirnas A,Andersson J,Singh V,Kk S,Johansson P,Ebenstein Y,Ambjörnsson T,Westerlund F

更新日期：2019-09-05 00:00:00

abstract：:Two CTAG-recognizing restriction and modification (R/M) systems, designated MthZI and MthFI, were identified in the thermophilic archaeon Methanobacterium thermoformicicum strains Z-245 and FTF, respectively. Further analysis revealed that the methyltransferase (MTase) genes are plasmid-located in both strains. The pl...

journal_title：Nucleic acids research

authors： Nölling J,de Vos WM

更新日期：1992-10-11 00:00:00

abstract：:The reaction of fluorescamine with primary amino groups of tRNAs was investigated. The reagent was attached under mild conditions to the 3'-end of tRNAPhe-C-C-A(3'NH) from yeast and to the minor nucleoside x in E. coli tRNAArg, tRNALys, tRNAMet, tRNAIle and tRNAPhe. The primary aliphatic amino groups of these tRNAs re...

journal_title：Nucleic acids research

authors： Sprinzl M,Faulhammer HG

更新日期：1978-12-01 00:00:00

abstract：:In vivo and in vitro RNA splicing experiments have demonstrated that the intron splicing machineries are not interchangeable in all organisms. These differences have prevented the efficient in vivo expression of monocot genes containing introns in dicot plants and the in vitro excision of some plant introns in HeLa ce...

journal_title：Nucleic acids research

authors： Hanley BA,Schuler MA

更新日期：1988-07-25 00:00:00

abstract：:A putative role for mammalian polynucleotide kinases that possess both 5'-phosphotransferase and 3'-phosphatase activity is the restoration of DNA strand breaks with 5'-hydroxyl termini or 3'-phosphate termini, or both, to a form that supports the subsequent action of DNA repair polymerases and DNA ligases, i.e. 5'-ph...

journal_title：Nucleic acids research

authors： Karimi-Busheri F,Lee J,Tomkinson AE,Weinfeld M

更新日期：1998-10-01 00:00:00

abstract：:Metal ions are essential for the folding of RNA into stable tertiary structures and for the catalytic activity of some RNA enzymes. To aid in the study of the roles of metal ions in RNA structural biology, we have created MeRNA (Metals in RNA), a comprehensive compilation of all metal binding sites identified in RNA 3...

journal_title：Nucleic acids research

authors： Stefan LR,Zhang R,Levitan AG,Hendrix DK,Brenner SE,Holbrook SR

更新日期：2006-01-01 00:00:00

abstract：:The cellular response to DNA double-strand breaks (DSBs) is initiated by the Mre11-Rad50-Xrs2 (MRX) complex that has structural and catalytic functions. MRX association at DSBs is counteracted by Rif2, which is known to interact with Rap1 that binds telomeric DNA through two tandem Myb-like domains. Whether and how Ra...

journal_title：Nucleic acids research

authors： Bonetti D,Rinaldi C,Vertemara J,Notaro M,Pizzul P,Tisi R,Zampella G,Longhese MP

更新日期：2020-03-18 00:00:00

abstract：:RNA-binding proteins (RBPs) play important roles in the post-transcriptional control of RNAs. Identifying RBP binding sites and characterizing RBP binding preferences are key steps toward understanding the basic mechanisms of the post-transcriptional gene regulation. Though numerous computational methods have been dev...

journal_title：Nucleic acids research

authors： Zhang S,Zhou J,Hu H,Gong H,Chen L,Cheng C,Zeng J

更新日期：2016-02-29 00:00:00

abstract：:The database of imprinted genes and parent-of-origin effects in animals (http://www.otago.ac.nz/IGC ) is a collation of genes and phenotypes for which parent-of-origin effects have been reported. The database currently includes over 220 entries, which describe over 40 imprinted genes in human, mouse and other animals....

journal_title：Nucleic acids research

authors： Morison IM,Paton CJ,Cleverley SD

更新日期：2001-01-01 00:00:00

abstract：:Replication of the promiscuous plasmid pMV158 requires expression of the initiator repB gene, which is controlled by the repressor CopG. Genes repB and copG are co-transcribed from promoter P(cr). We have studied the interactions between RNA polymerase, CopG and the promoter to elucidate the mechanism of repression by...

journal_title：Nucleic acids research

authors： Hernández-Arriaga AM,Rubio-Lepe TS,Espinosa M,del Solar G

更新日期：2009-08-01 00:00:00

abstract：:Controller (C) proteins regulate the timing of the expression of restriction and modification (R-M) genes through a combination of positive and negative feedback circuits. A single dimer bound to the operator switches on transcription of the C-gene and the endonuclease gene; at higher concentrations, a second dimer bo...

journal_title：Nucleic acids research

authors： McGeehan JE,Streeter SD,Thresh SJ,Ball N,Ravelli RB,Kneale GG

更新日期：2008-08-01 00:00:00

abstract：:Most proteins from higher organisms are known to be multi-domain proteins and contain substantial numbers of intrinsically disordered (ID) regions. To analyse such protein sequences, those from human for instance, we developed a special protein-structure-prediction pipeline and accumulated the products in the Structur...

journal_title：Nucleic acids research

authors： Motono C,Nakata J,Koike R,Shimizu K,Shirota M,Amemiya T,Tomii K,Nagano N,Sakaya N,Misoo K,Sato M,Kidera A,Hiroaki H,Shirai T,Kinoshita K,Noguchi T,Ota M

更新日期：2011-01-01 00:00:00

abstract：:A 15-mer DNA aptamer (named TBA) adopts a G-quadruplex structure that strongly inhibits fibrin-clot formation by binding to thrombin. We have performed thermodynamic analysis, binding affinity and biological activity studies of TBA variants modified by unlocked nucleic acid (UNA) monomers. UNA-U placed in position U3,...

journal_title：Nucleic acids research

authors： Pasternak A,Hernandez FJ,Rasmussen LM,Vester B,Wengel J

更新日期：2011-02-01 00:00:00

abstract：:Single-walled carbon nanotubes (SWNTs) have been considered as the leading candidate for nanodevice applications ranging from gene therapy and novel drug delivery to membrane separations. The miniaturization of DNA-nanotube devices for biological applications requires fully understanding DNA-nanotube interaction mecha...

journal_title：Nucleic acids research

authors： Li X,Peng Y,Qu X

更新日期：2006-08-02 00:00:00

abstract：:The Neurospora VS ribozyme is a catalytic RNA that has the unique ability to specifically recognize and cleave a stem-loop substrate through formation of a highly stable kissing-loop interaction (KLI). In order to explore the engineering potential of the VS ribozyme to cleave alternate substrates, we substituted the w...

journal_title：Nucleic acids research

authors： Lacroix-Labonté J,Girard N,Dagenais P,Legault P

更新日期：2016-08-19 00:00:00

abstract：:Structural Maintenance of Chromosomes (SMC) proteins are vital for a wide range of processes including chromosome structure and dynamics, gene regulation and DNA repair. Eukaryotes have three SMC complexes, consisting of heterodimeric pairs of six different SMC proteins along with several specific regulatory subunits....

journal_title：Nucleic acids research

authors： Griese JJ,Witte G,Hopfner KP

更新日期：2010-06-01 00:00:00

abstract：:We have previously described a partial Drosophila cDNA, clone P19, which bears homology to members of the RNA recognition motif (RRM) family of proteins [Haynes et al. (1987) Proc. Natl. Acad. Sci. USA, 84, 1819-1823]. RNA binding as well as involvement in RNA processing has been demonstrated for some RRM proteins. We...

journal_title：Nucleic acids research

authors： Stolow DT,Haynes SR

更新日期：1995-03-11 00:00:00

abstract：:The molecular mechanism in pancreatic β cells underlying hyperlipidemia and insulin insufficiency remains unclear. Here, we find that the fatty acid-induced decrease in insulin levels occurs due to a decrease in insulin translation. Since regulation at the translational level is generally mediated through RNA-binding ...

journal_title：Nucleic acids research

authors： Li Z,Zhou M,Cai Z,Liu H,Zhong W,Hao Q,Cheng D,Hu X,Hou J,Xu P,Xue Y,Zhou Y,Xu T

更新日期：2018-12-14 00:00:00

abstract：:Initiation of bacterial DNA replication at oriC is mediated by primosomal proteins that act cooperatively to melt an AT-rich region where the replicative helicase is loaded prior to the assembly of the replication fork. In Bacillus subtilis, the dnaD, dnaB and dnaI genes are essential for initiation of DNA replication...

journal_title：Nucleic acids research

authors： Grainger WH,Machón C,Scott DJ,Soultanas P

更新日期：2010-05-01 00:00:00

abstract：:To gain insight into the mechanistic link between translation termination and nonsense-mediated mRNA decay (NMD), we depleted the ribosome recycling factor ABCE1 in human cells, resulting in an upregulation of NMD-sensitive mRNAs. Suppression of NMD on these mRNAs occurs prior to their SMG6-mediated endonucleolytic cl...

journal_title：Nucleic acids research

authors： Annibaldis G,Domanski M,Dreos R,Contu L,Carl S,Kläy N,Mühlemann O

更新日期：2020-10-09 00:00:00

abstract：:New linker phosphoramidite reagents containing a cleavable 3'-ester linkage are used for attaching the first nucleoside to the surface of a solid- phase support. Inexpensive, underivatized amino supports, such as long chain alkylamine controlled-pore glass, can serve as universal supports. No modifications to phosphor...

journal_title：Nucleic acids research

authors： Pon RT,Yu S

更新日期：2004-01-29 00:00:00

abstract：:Chromatin immunoprecipitation (ChIP) has ushered in a new era of scientific discovery by allowing new insights into DNA-protein interactions. ChIP is used to quantify enriched genomic regions using qPCR, and more recently is combined with next generation sequencing (ChIP-seq) to obtain a genome wide profile of protein...

journal_title：Nucleic acids research

authors： Kurtenbach S,Reddy R,Harbour JW

更新日期：2019-01-08 00:00:00

abstract：:The small proline-rich protein genes ( SPRRs ) code for precursors of the cornified cell envelope, and are specifically expressed during keratinocyte terminal differentiation. The single intron of SPRR2A enhanced the activity of the SPRR2A promoter in transient transfection assays. This enhancement was position depend...

journal_title：Nucleic acids research

authors： Fischer DF,van Drunen CM,Winkler GS,van de Putte P,Backendorf C

更新日期：1998-12-01 00:00:00

abstract：:We describe the characterisation of four thermo-stable NAD(+)-dependent DNA ligases, from Thermus thermophilus (Tth), Thermus scotoductus (Ts), Rhodothermus marinus (Rm) and Thermus aquaticus (Taq), by an assay which measures ligation rate and mismatch discrimination. Complete libraries of octa-, nona- and decanucleot...

journal_title：Nucleic acids research

authors： Housby JN,Thorbjarnardóttir SH,Jónsson ZO,Southern EM

更新日期：2000-02-01 00:00:00

abstract：:The Comparative Toxicogenomics Database (CTD; http://ctdbase.org/) is a premier public resource for literature-based, manually curated associations between chemicals, gene products, phenotypes, diseases, and environmental exposures. In this biennial update, we present our new chemical-phenotype module that codes chemi...

journal_title：Nucleic acids research

authors： Davis AP,Grondin CJ,Johnson RJ,Sciaky D,McMorran R,Wiegers J,Wiegers TC,Mattingly CJ

更新日期：2019-01-08 00:00:00

abstract：:Minichromosome maintenance (MCM) proteins facilitate replication by licensing origins and unwinding the DNA double strand. Interestingly, the number of MCM hexamers greatly exceeds the number of firing origins suggesting additional roles of MCMs. Here we show a hitherto unanticipated function of MCM2 in cilia formatio...

journal_title：Nucleic acids research

authors： Casar Tena T,Maerz LD,Szafranski K,Groth M,Blätte TJ,Donow C,Matysik S,Walther P,Jeggo PA,Burkhalter MD,Philipp M

更新日期：2019-01-10 00:00:00