Synthesis and hydrolysis of oligodeoxyribonucleotides containing 2-aminopurine.


:A new method is reported for the synthesis of oligodeoxyribonucleotides containing 2-aminopurine residues at selected sites. This method involves protection of the 2-aminopurine ribonucleoside, reduction to the deoxyribonucleoside and standard preparation of the 5'-0- (4,4'-dimethoxytrityl)-3'-O-(2-cyanoethyl)-N,N- diisopropylphosphoramidite. The 2-aminopurine phosphoramidite prepared by this method couples with high efficiency and is stable under standard automated synthesis conditions. The presence and location of the 2-aminopurine residue is easily verified by treatment of the oligodeoxyribonucleotide with hot piperidine. The mechanism for selective hydrolysis of the 2-aminopurine residue in alkaline solution is predominantly direct cleave of the glycosidic bond.


Nucleic Acids Res


Nucleic acids research


Fujimoto J,Nuesca Z,Mazurek M,Sowers LC




Has Abstract


1996-02-15 00:00:00














  • Crystal structure of Hfq from Bacillus subtilis in complex with SELEX-derived RNA aptamer: insight into RNA-binding properties of bacterial Hfq.

    abstract::Bacterial Hfq is a protein that plays an important role in the regulation of genes in cooperation with sRNAs. Escherichia coli Hfq (EcHfq) has two or more sites that bind RNA(s) including U-rich and/or the poly(A) tail of mRNA. However, functional and structural information about Bacillus subtilis Hfq (BsHfq) includin...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Someya T,Baba S,Fujimoto M,Kawai G,Kumasaka T,Nakamura K

    更新日期:2012-02-01 00:00:00

  • The use of R-looping for structural gene identification and mRNA purification.

    abstract::A method is presented for the purification of mRNAs and the identification of structural gene sequences in recombinant DNA molecules. RNA is hybridized to double-stranded linear DNA such that R-loops are formed between most DNAs and their complementary RNA sequences. These R-loops are purified from unhybridized RNAs b...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Woolford JL Jr,Rosbash M

    更新日期:1979-06-11 00:00:00

  • Ultraviolet resonance Raman study of DNA and of its interaction with actinomycin D.

    abstract::The DNA-Actinomycin D interaction has been studied by resonance Raman effect using DNA as chromophore. First, the resonance Raman spectra of DNA obtained with a U.V. excitation at wavelengths of 300 nm and 280 nm are presented. The main Raman hands are assigned to the convenient nucleic bases by comparison with the sp...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Chinsky L,Turpin PY

    更新日期:1978-08-01 00:00:00

  • New rapid methods for DNA sequencing based in exonuclease III digestion followed by repair synthesis.

    abstract::We describe improve enzymatic methods for sequencing method for sequencing DNA. They are based on partial digestion of duplex DNA with exonuclease III to produce DNA molecules with 3' ends shortened to varying lengths, followed by repair synthesis to extend and label the 3' ends. After asymmetrical cleavage of the DNA...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Guo LH,Wu R

    更新日期:1982-03-25 00:00:00

  • Genomic SELEX for Hfq-binding RNAs identifies genomic aptamers predominantly in antisense transcripts.

    abstract::An unexpectedly high number of regulatory RNAs have been recently discovered that fine-tune the function of genes at all levels of expression. We employed Genomic SELEX, a method to identify protein-binding RNAs encoded in the genome, to search for further regulatory RNAs in Escherichia coli. We used the global regula...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Lorenz C,Gesell T,Zimmermann B,Schoeberl U,Bilusic I,Rajkowitsch L,Waldsich C,von Haeseler A,Schroeder R

    更新日期:2010-06-01 00:00:00

  • TRANSFAC, TRRD and COMPEL: towards a federated database system on transcriptional regulation.

    abstract::Three databases that provide data on transcriptional regulation are described. TRANSFAC is a database on transcription factors and their DNA binding sites. TRRD (Transcription Regulatory Region Database) collects information about complete regulatory regions, their regulation properties and architecture. COMPEL compri...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Wingender E,Kel AE,Kel OV,Karas H,Heinemeyer T,Dietze P,Knüppel R,Romaschenko AG,Kolchanov NA

    更新日期:1997-01-01 00:00:00

  • DNA synthesis is initiated at two positions within the origin of replication of plasmid R1162.

    abstract::DNA synthesis of broad host-range plasmid R1162 is initiated from two positions, flanking a large (40 bp stem, 40 bp loop) inverted repeat. Each start-point is located within a highly conserved, but oppositely oriented, 10 base-pair sequence. Synthesis from the two positions converges within the intervening inverted r...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Lin LS,Meyer RJ

    更新日期:1987-10-26 00:00:00

  • Programmed genome rearrangements in Oxytricha produce transcriptionally active extrachromosomal circular DNA.

    abstract::Extrachromosomal circular DNA (eccDNA) is both a driver of eukaryotic genome instability and a product of programmed genome rearrangements, but its extent had not been surveyed in Oxytricha, a ciliate with elaborate DNA elimination and translocation during development. Here, we captured rearrangement-specific circular...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Yerlici VT,Lu MW,Hoge CR,Miller RV,Neme R,Khurana JS,Bracht JR,Landweber LF

    更新日期:2019-10-10 00:00:00

  • Structural analysis of an RNA molecule involved in replication control of plasmid R1.

    abstract::The replication control circuit of the FII plasmids R1, R100 and R6-5 involves a small untranslated RNA molecule of about 93 nucleotides. This RNA, designated CopA RNA in plasmid R1, acts in vivo as a post-transcriptional inhibitor of the expression of the RepA protein whose synthesis is required for each round of pla...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Gerhart E,Wagner H,Nordström K

    更新日期:1986-03-25 00:00:00

  • Promoter selectivity of the stationary-phase forms of Escherichia coli RNA polymerase and conversion in vitro of the S1 form enzyme into a log-phase enzyme-like form.

    abstract::Upon growth transition of Escherichia coli cells from exponential to stationary phase, RNA polymerase is converted into at least three different forms (S1, S2 and S3), which could be separately isolated by phosphocellulose column chromatography (Ozaki et al., 1991 (2)). Here, the promoter selectivity of these three st...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Ozaki M,Fujita N,Wada A,Ishihama A

    更新日期:1992-01-25 00:00:00

  • Joint annotation of coding and non-coding single nucleotide polymorphisms and mutations in the SNPeffect and PupaSuite databases.

    abstract::Single nucleotide polymorphisms (SNPs) are, together with copy number variation, the primary source of variation in the human genome. SNPs are associated with altered response to drug treatment, susceptibility to disease and other phenotypic variation. Furthermore, during genetic screens for disease-associated mutatio...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Reumers J,Conde L,Medina I,Maurer-Stroh S,Van Durme J,Dopazo J,Rousseau F,Schymkowitz J

    更新日期:2008-01-01 00:00:00

  • Alternative splicing of beta-tropomyosin pre-mRNA: multiple cis-elements can contribute to the use of the 5'- and 3'-splice sites of the nonmuscle/smooth muscle exon 6.

    abstract::We previously found that the splicing of exon 5 to exon 6 in the rat beta-TM gene required that exon 6 first be joined to the downstream common exon 8 (Helfman et al., Genes and Dev. 2, 1627-1638, 1988). Pre-mRNAs containing exon 5, intron 5 and exon 6 are not normally spliced in vitro. We have carried out a mutationa...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Tsukahara T,Casciato C,Helfman DM

    更新日期:1994-06-25 00:00:00

  • A non-canonical multisubunit RNA polymerase encoded by the AR9 phage recognizes the template strand of its uracil-containing promoters.

    abstract::AR9 is a giant Bacillus subtilis phage whose uracil-containing double-stranded DNA genome encodes distant homologs of β and β' subunits of bacterial RNA polymerase (RNAP). The products of these genes are thought to assemble into two non-canonical multisubunit RNAPs - a virion RNAP (vRNAP) that is injected into the hos...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Sokolova M,Borukhov S,Lavysh D,Artamonova T,Khodorkovskii M,Severinov K

    更新日期:2017-06-02 00:00:00

  • StreptomeDB: a resource for natural compounds isolated from Streptomyces species.

    abstract::Bacteria from the genus Streptomyces are very important for the production of natural bioactive compounds such as antibiotic, antitumour or immunosuppressant drugs. Around two-thirds of all known natural antibiotics are produced by these bacteria. An enormous quantity of crucial data related to this genus has been gen...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Lucas X,Senger C,Erxleben A,Grüning BA,Döring K,Mosch J,Flemming S,Günther S

    更新日期:2013-01-01 00:00:00

  • SPRING: a tool for the analysis of genome rearrangement using reversals and block-interchanges.

    abstract::SPRING ( is a tool for the analysis of genome rearrangement between two chromosomal genomes using reversals and/or block-interchanges. SPRING takes two or more chromosomes as its input and then computes a minimum series of reversals and/or block-interchanges between any tw...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Lin YC,Lu CL,Liu YC,Tang CY

    更新日期:2006-07-01 00:00:00

  • Sequence complexity and diversity of polyadenylated RNA molecules of human normal resting, PHA-stimulated and leukemic lymphocytes.

    abstract::The abundance and complexity of total poly(A)+RNA of human normal unstimulated lymphocytes, PHA-stimulated and leukemic lymphocytes were examined using the cDNA-poly(A)+ RNA hybridization technique. It was found that in both PHA-stimulated and leukemic lymphocytes the complexity of total poly(A)+RNA is strongly reduce...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Torelli G,Ferrari S,Donelli A,Cadossi R,Ferrari S,Bosi P,Torelli U

    更新日期:1981-12-21 00:00:00

  • Sequence-specific minor groove binding by bis-benzimidazoles: water molecules in ligand recognition.

    abstract::The binding of two symmetric bis-benzimidazole compounds, 2,2-bis-[4'-(3"-dimethylamino-1"-propyloxy)phenyl]-5,5-bi-1H-benzimidazole and its piperidinpropylphenyl analog, to the minor groove of DNA, have been studied by DNA footprinting, surface plasmon resonance (SPR) methods and molecular dynamics simulations in exp...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Bailly C,Chessari G,Carrasco C,Joubert A,Mann J,Wilson WD,Neidle S

    更新日期:2003-03-01 00:00:00

  • 3CAPS - a structural AP-site analogue as a tool to investigate DNA base excision repair.

    abstract::Abasic sites (AP-sites) are frequent DNA lesions, arising by spontaneous base hydrolysis or as intermediates of base excision repair (BER). The hemiacetal at the anomeric centre renders them chemically reactive, which presents a challenge to biochemical and structural investigation. Chemically more stable AP-site anal...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Schuermann D,Scheidegger SP,Weber AR,Bjørås M,Leumann CJ,Schär P

    更新日期:2016-03-18 00:00:00

  • Denaturation maps of DNA: experimental and theoretical maps of phiX174 DNA.

    abstract::A formaldehyde denaturation map of the replicative form of phiX174 DNA is obtained. The RFI DNA was converted into a linear state by restriction endonuclease pst I which introduces into this DNA a single double-stranded break. The map has four clear-cut peaks. Their positions excellently correlate with the peak positi...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Karataev GI,Permogorov VI,Vologodskii AV,Frank-Kamenetskii MD

    更新日期:1978-07-01 00:00:00

  • Cytotoxic and mutagenic properties of alkyl phosphotriester lesions in Escherichia coli cells.

    abstract::Exposure to many endogenous and exogenous agents can give rise to DNA alkylation, which constitutes a major type of DNA damage. Among the DNA alkylation products, alkyl phosphotriesters have relatively high frequencies of occurrence and are resistant to repair in mammalian tissues. However, little is known about how t...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Wu J,Wang P,Wang Y

    更新日期:2018-05-04 00:00:00

  • Sequence-specific functions of the early palindrome domain within the SV40 core origin of replication.

    abstract::The early palindrome domain within the SV40 core origin of replication is essential for the initiation of replication. Studies with single point mutants in this region suggested that the early palindrome domain does not function as a cruciform structure, but may be involved in the initiation of SV40 DNA replication in...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Kim YS,Kang HS

    更新日期:1989-11-25 00:00:00

  • EcoGene 3.0.

    abstract::EcoGene ( is a database and website devoted to continuously improving the structural and functional annotation of Escherichia coli K-12, one of the most well understood model organisms, represented by the MG1655(Seq) genome sequence and annotations. Major improvements to EcoGene in the past decade i...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Zhou J,Rudd KE

    更新日期:2013-01-01 00:00:00

  • Transcript quantitation in total yeast cellular RNA using kinetic PCR.

    abstract::Kinetically monitored, reverse transcriptase-initiated PCR (kinetic RT-PCR, kRT-PCR) is a novel application of kinetic PCR for high throughput transcript quantitation in total cellular RNA. The assay offers the simplicity and flexibility of an enzyme assay with distinct advantages over DNA microarray hybridization and...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Kang JJ,Watson RM,Fisher ME,Higuchi R,Gelfand DH,Holland MJ

    更新日期:2000-01-15 00:00:00

  • Hormonal regulation of transcription of rDNA: use of nucleoside thiotriphosphates to measure initiation in isolated nuclei.

    abstract::Nuclei isolated from cultured mouse and rat cell lines initiated pre-rRNA chains at the correct site and with the correct nucleotide specificity (A for mouse, G for rat). Nucleic acid filter hybridization and S1 nuclease mapping were used to analyze the RNA products initiated with nucleoside (beta-S)triphosphates. Ini...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Zhang ZY,Thompson EA,Stallcup MR

    更新日期:1984-11-12 00:00:00

  • Cycling of the E. coli lagging strand polymerase is triggered exclusively by the availability of a new primer at the replication fork.

    abstract::Two models have been proposed for triggering release of the lagging strand polymerase at the replication fork, enabling cycling to the primer for the next Okazaki fragment--either collision with the 5'-end of the preceding fragment (collision model) or synthesis of a new primer by primase (signaling model). Specific p...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Yuan Q,McHenry CS

    更新日期:2014-02-01 00:00:00

  • Content discovery and retrieval services at the European Nucleotide Archive.

    abstract::The European Nucleotide Archive (ENA; is Europe's primary resource for nucleotide sequence information. With the growing volume and diversity of public sequencing data comes the need for increased sophistication in data organisation, presentation and search services so as to maximise its disc...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Silvester N,Alako B,Amid C,Cerdeño-Tárraga A,Cleland I,Gibson R,Goodgame N,Ten Hoopen P,Kay S,Leinonen R,Li W,Liu X,Lopez R,Pakseresht N,Pallreddy S,Plaister S,Radhakrishnan R,Rossello M,Senf A,Smirnov D,Toribio A

    更新日期:2015-01-01 00:00:00

  • Interaction of the resolving enzyme YDC2 with the four-way DNA junction.

    abstract::Holliday junctions (four-way DNA junctions), formed during homologous recombination, are bound and resolved by junction-specific endonucleases to yield recombinant duplex DNA products. The junction-resolving enzymes are a structurally diverse class of proteins that nevertheless have many properties in common; in parti...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: White MF,Lilley DM

    更新日期:1998-12-15 00:00:00

  • Comparative genomic analysis of equilibrative nucleoside transporters suggests conserved protein structure despite limited sequence identity.

    abstract::Equilibrative nucleoside transporters (ENTs) are a recently characterized and poorly understood group of membrane proteins that are important in the uptake of endogenous nucleosides required for nucleic acid and nucleoside triphosphate synthesis. Despite their central importance in cellular metabolism and nucleoside a...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Sankar N,Machado J,Abdulla P,Hilliker AJ,Coe IR

    更新日期:2002-10-15 00:00:00

  • On the fidelity of DNA replication: herpes DNA polymerase and its associated exonuclease.

    abstract::Procaryotic DNA polymerases contain an associated 3'----5' exonuclease activity which provides a proofreading function and contributes substantially to replication fidelity. DNA polymerases of the eucaryotic herpes-type viruses contain similar associated exonuclease activities. We have investigated the fidelity of pol...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Abbotts J,Nishiyama Y,Yoshida S,Loeb LA

    更新日期:1987-02-11 00:00:00

  • Cell-free cloning of highly expanded CTG repeats by amplification of dimerized expanded repeats.

    abstract::We describe conditions for producing uninterrupted expanded CTG repeats consisting of up to 2000 repeats using 29 DNA polymerase. Previously, generation of such repeats was hindered by CTG repeat instability in plasmid vectors maintained in Escherichia coli and poor in vitro ligation of CTG repeat concatemers due to s...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Osborne RJ,Thornton CA

    更新日期:2008-03-01 00:00:00