New efficient sulfurizing reagents for the preparation of oligodeoxyribonucleotide phosphorothioate analogues.

abstract：:Structural changes of reconstituted SV 40 minichromosomes have been studied in relation to the salt concentration and addition of histone H1 by sedimentation and electron microscopy. Sedimentation data are represented as functions of the NaCl concentration and the Debye-Hückel electrostatic screening radius 1/alpha. T...

journal_title：Nucleic acids research

authors： Böttger M,Scherneck S,von Mickwitz CU,Fenske H,Lindigkeit R

更新日期：1979-08-10 00:00:00

abstract：:We present a study of how substituent groups of naturally occurring and modified nucleotide bases affect the degree of hydration of right-handed B-DNA and left-handed Z-DNA. A comparison of poly(dG-dC) and poly(dG-dm5C) titrations with the lipotropic salts of the Hofmeister series infers that the methyl stabilization ...

journal_title：Nucleic acids research

authors： Kagawa TF,Howell ML,Tseng K,Ho PS

更新日期：1993-12-25 00:00:00

abstract：:ATR functions as a master regulator of the DNA-damage response. ATR activation requires the ATR activator, topoisomerase IIβ-binding protein 1 (TopBP1). However, the underlying mechanism of TopBP1 regulation and how its regulation affects DNA replication remain unknown. Here, we report a specific interaction between T...

journal_title：Nucleic acids research

authors： Feng H,Lu J,Song X,Thongkum A,Zhang F,Lou L,Reizes O,Almasan A,Gong Z

更新日期：2020-11-04 00:00:00

abstract：:Release of RNA polymerase II (Pol II) from promoter-proximal pausing has emerged as a critical step regulating gene expression in multicellular organisms. The transition of Pol II into productive elongation requires the kinase activity of positive transcription elongation factor b (P-TEFb), which is itself under a str...

journal_title：Nucleic acids research

authors： C Quaresma AJ,Bugai A,Barboric M

更新日期：2016-09-19 00:00:00

abstract：:The "ovalbumin Y" gene, one of three which constitute the ovalbumin gene family in chicken has been completely sequenced. The exact location of exons can be derived from the comparison with the ovalbumin gene sequence and from the map previously established by electron microscopy analysis. During evolution of the Y ge...

journal_title：Nucleic acids research

authors： Heilig R,Muraskowsky R,Kloepfer C,Mandel JL

更新日期：1982-07-24 00:00:00

abstract：:A problem in EST clustering is the presence of repeat sequences. To avoid false matches, repeats have to be masked. This can be a time-consuming process, and it depends on available repeat libraries. We present a fast and effective method that aims to eliminate the problems repeats cause in the process of clustering. ...

journal_title：Nucleic acids research

authors： Schneeberger K,Malde K,Coward E,Jonassen I

更新日期：2005-04-14 00:00:00

abstract：:Vascular endothelial growth factor A (VEGF-A) is a master regulator of angiogenesis, vascular development and function. In this study we investigated the transcriptional regulation of VEGF-A-responsive genes in primary human aortic endothelial cells (HAECs) and human umbilical vein endothelial cells (HUVECs) using gen...

journal_title：Nucleic acids research

authors： Kaikkonen MU,Niskanen H,Romanoski CE,Kansanen E,Kivelä AM,Laitalainen J,Heinz S,Benner C,Glass CK,Ylä-Herttuala S

更新日期：2014-11-10 00:00:00

abstract：:This paper describes a method for predicting a supersecondary structural motif, beta-hairpins, in a protein sequence. The method was trained and tested on a set of 5102 hairpins and 5131 non-hairpins, obtained from a non-redundant dataset of 2880 proteins using the DSSP and PROMOTIF programs. Two machine-learning tech...

journal_title：Nucleic acids research

authors： Kumar M,Bhasin M,Natt NK,Raghava GP

更新日期：2005-07-01 00:00:00

abstract：:We have investigated the distribution of mitochondrial DNA polymorphisms in a rare maternally transmitted genetic trait that causes hypersensitivity to aminoglycoside antibiotics, in the hope that a characterization of its molecular basis might provide a molecular and cellular understanding of aminoglycoside-induced d...

journal_title：Nucleic acids research

authors： Hutchin T,Haworth I,Higashi K,Fischel-Ghodsian N,Stoneking M,Saha N,Arnos C,Cortopassi G

更新日期：1993-09-11 00:00:00

abstract：:During the last years, the demand for custom-made cDNA chips/arrays as well as whole genome chips is increasing rapidly. The efficient selection of gene-specific primers/oligomers is of the utmost importance for the successful production of such chips. We developed GenomePRIDE, a highly flexible and scalable software ...

journal_title：Nucleic acids research

authors： Haas SA,Hild M,Wright AP,Hain T,Talibi D,Vingron M

更新日期：2003-10-01 00:00:00

abstract：:SynergyFinder (https://synergyfinder.fimm.fi) is a stand-alone web-application for interactive analysis and visualization of drug combination screening data. Since its first release in 2017, SynergyFinder has become a widely used web-tool both for the discovery of novel synergistic drug combinations in pre-clinical mo...

journal_title：Nucleic acids research

authors： Ianevski A,Giri AK,Aittokallio T

更新日期：2020-07-02 00:00:00

abstract：:The x-ray crystal structures of two new anti-HIV compounds, 9-(2,3-dideoxy-2-fluoro-beta-D-threo-pentofuranosyl)adenine (2'-F-dd-araA) and 9-(2,3-dideoxy-2-fluoro-beta-D-threo- pentofuranosyl)hypoxanthine (2'-F-dd-aral), have been determined at two temperatures. Both crystals are in the space group P2(1)2(1)2(1), and ...

journal_title：Nucleic acids research

authors： Liaw YC,Gao YG,Marquez VE,Wang AH

更新日期：1992-02-11 00:00:00

abstract：:Protein synthesis elongation factor 2 (EF-2) from all archaebacteria so far analysed, is susceptible to inactivation by diphtheria toxin, a property which it shares with EF-2 from the eukaryotic 8OS translation system. To resolve the structural basis of diphtheria toxin susceptibility, the structural gene for the EF-2...

journal_title：Nucleic acids research

authors： Lechner K,Heller G,Böck A

更新日期：1988-08-25 00:00:00

abstract：:Heterotrimeric eukaryotic/archaeal translation initiation factor 2 (e/aIF2) binds initiator methionyl-tRNA and plays a key role in the selection of the start codon on messenger RNA. tRNA binding was extensively studied in the archaeal system. The γ subunit is able to bind tRNA, but the α subunit is required to reach h...

journal_title：Nucleic acids research

authors： Naveau M,Lazennec-Schurdevin C,Panvert M,Dubiez E,Mechulam Y,Schmitt E

更新日期：2013-01-01 00:00:00

abstract：:Thyroglobulin (Tg), the precursor of thyroid hormones, is a 660.000 Da dimeric glycoprotein synthesized exclusively in the thyroid gland. We have cloned the human thyroglobulin gene from cosmid and phage libraries and constructed a complete restriction map. The gene encodes an 8.7 kb mRNA, covers at least 300 kb DNA a...

journal_title：Nucleic acids research

authors： Baas F,van Ommen GJ,Bikker H,Arnberg AC,de Vijlder JJ

更新日期：1986-07-11 00:00:00

abstract：:Homologous recombination (HR) is important in repairing errors of replication and other forms of DNA damage. In mammalian cells, potential templates include the homologous chromosome, and after DNA replication, the sister chromatid. Previous work has shown that the mammalian recombination machinery is organized to sup...

journal_title：Nucleic acids research

authors： Read LR,Raynard SJ,Rukść A,Baker MD

更新日期：2004-02-20 00:00:00

abstract：:Mismatch uracil DNA glycosylase (Mug) from Escherichia coli is an initiating enzyme in the base-excision repair pathway. As with other DNA glycosylases, the abasic product is potentially more harmful than the initial lesion. Since Mug is known to bind its product tightly, inhibiting enzyme turnover, understanding how ...

journal_title：Nucleic acids research

authors： Grippon S,Zhao Q,Robinson T,Marshall JJ,O'Neill RJ,Manning H,Kennedy G,Dunsby C,Neil M,Halford SE,French PM,Baldwin GS

更新日期：2011-04-01 00:00:00

abstract：:For the rapid and sensitive detection of p53 'hot spot' mutations, we combined polymerase chain reaction based single-strand conformational polymorphism (PCR-SSCP) analysis with sequence specific-clamping by peptide nucleic acids (PNAs) in a one-step reaction tube protocol. For this purpose, we designed two PNA molecu...

journal_title：Nucleic acids research

authors： Behn M,Schuermann M

更新日期：1998-03-01 00:00:00

abstract：:The nuclear suppressor allele NSM3 in strain FF1210-6C/170-E22 (E22), which suppresses a mutation of the yeast mitochondrial tRNA(Asp)gene in Saccharomyces cerevisiae, was cloned and identified. To isolate the NSM3 allele, a genomic DNA library using the vector YEp13 was constructed from strain E22. Nine YEp13 recombi...

journal_title：Nucleic acids research

authors： Chiang CS,Liaw GJ

更新日期：2000-04-01 00:00:00

abstract：:SMILE (small heterodimer partner interacting leucine zipper protein) has been identified as a coregulator in ER signaling. In this study, we have examined the effects of SMILE on other NRs (nuclear receptors). SMILE inhibits GR, CAR and HNF4 alpha-mediated transactivation. Knockdown of SMILE gene expression increases ...

journal_title：Nucleic acids research

authors： Xie YB,Nedumaran B,Choi HS

更新日期：2009-07-01 00:00:00

abstract：:Translesional DNA synthesis past abasic sites proceeds with the preferential incorporation of dAMP opposite the lesion and, depending on the sequence context, one or two base deletions. High-resolution NMR spectroscopy and molecular dynamics simulations were used to determine the three-dimensional structure of a DNA h...

journal_title：Nucleic acids research

authors： Lin Z,Hung KN,Grollman AP,de los Santos C

更新日期：1998-05-15 00:00:00

abstract：:Non-radioactive nucleic acid hybridization probes have been constructed in which the reporter group is long chain biotin chemically linked to a basic macromolecule (histone H1, cytochrome C or polyethyleneimine). The modified basic macromolecule which carries many biotin residues can, in turn, be covalently linked to ...

journal_title：Nucleic acids research

authors： Al-Hakim AH,Hull R

更新日期：1986-12-22 00:00:00

abstract：:Preparation of large amount of single-stranded circular DNA in high selectivity is crucial for further developments of nanotechnology and other DNA sciences. Herein, a simple but practically useful methodology to prepare DNA rings has been presented. One of the essential factors is to use highly diluted T4 ligase buff...

journal_title：Nucleic acids research

authors： An R,Li Q,Fan Y,Li J,Pan X,Komiyama M,Liang X

更新日期：2017-09-06 00:00:00

abstract：:Allele-specific protospacer adjacent motif (asPAM)-positioning SNPs and CRISPRs are valuable resources for gene therapy of dominant disorders. However, one technical hurdle is to identify the haplotype comprising the disease-causing allele and the distal asPAM SNPs. Here, we describe a novel CRISPR-based method (CRISP...

journal_title：Nucleic acids research

authors： Yu J,Xiang X,Huang J,Liang X,Pan X,Dong Z,Petersen TS,Qu K,Yang L,Zhao X,Li S,Zheng T,Xu Z,Liu C,Han P,Xu F,Yang H,Liu X,Zhang X,Bolund L,Luo Y,Lin L

更新日期：2020-03-18 00:00:00

abstract：:The three-dimensional Poisson-Boltzmann equation for the distribution of counterion charge density around double helical DNA has been solved by an iterative procedure. These computations have been performed for 0.01M monovalent salt solutions. A systematic study of the ten possible sequences found among adjacent nucle...

journal_title：Nucleic acids research

authors： Pack GR,Wong L,Prasad CV

更新日期：1986-02-11 00:00:00

abstract：:A novel method for sequence specific double strand DNA cleavage using PNA (peptide nucleic acid) targeting is described. Nuclease S1 digestion of double stranded DNA gives rise to double strand cleavage at an occupied PNA strand displacement binding site, and under optimized conditions complete cleavage can be obtaine...

journal_title：Nucleic acids research

authors： Demidov V,Frank-Kamenetskii MD,Egholm M,Buchardt O,Nielsen PE

更新日期：1993-05-11 00:00:00

abstract：:As the largest group of MYB family transcription factors, R2R3-MYB proteins play essential roles during plant growth and development. However, the structural basis underlying how R2R3-MYBs recognize the target DNA remains elusive. Here, we report the crystal structure of Arabidopsis WEREWOLF (WER), an R2R3-MYB protein...

journal_title：Nucleic acids research

authors： Wang B,Luo Q,Li Y,Yin L,Zhou N,Li X,Gan J,Dong A

更新日期：2020-01-10 00:00:00

abstract：:The 4.5S rRNA was isolated from the chloroplast ribosomes from Dryopteris acuminata. The complete nucleotide sequence was determined to be: OHUAAGGUCACGGCAAGACGAGCCGUUUAUCACCACGAUAGGUGCUAAGUGGAGGUGCAGUAAUGUAUGCAGCUGAGGC AUCCUAAUAGACCGAGAGGUUUGAACOH. The 4.5S rRNA is composed of 103 nucleotides and shows strong homolog...

journal_title：Nucleic acids research

authors： Takaiwa F,Kusuda M,Sugiura M

更新日期：1982-04-10 00:00:00

abstract：:The 3' splice site of the second intron (I2) of the human apolipoprotein-AII gene, (GT)16GGGCAG, is unique in that, although fully functional, a stretch of alternating guanine and thymine residues replaces the polypyrimidine tract usually associated with 3' splice junctions. The transient expression of successive 5' d...

journal_title：Nucleic acids research

authors： Shelley CS,Baralle FE

更新日期：1987-05-11 00:00:00

abstract：:Initiation of Adenovirus (Ad) DNA replication occurs by a protein-priming mechanism in which the viral precursor terminal protein (pTP) and DNA polymerase (pol) as well as two nuclear DNA-binding proteins from uninfected HeLa cells are required. Biochemical studies on the pTP and DNA polymerase proteins separately hav...

journal_title：Nucleic acids research

authors： Stunnenberg HG,Lange H,Philipson L,van Miltenburg RT,van der Vliet PC

更新日期：1988-03-25 00:00:00