The nucleotide sequence of chloroplast 4.5S rRNA from a fern, Dryopteris acuminata.

abstract：:We have sequenced mouse tRNA genes from two recombinant lambda phage. An 1800 bp sequence from one phage contains 3 tRNA genes, potentially encoding tRNAAsp, tRNAGly, and tRNAGlu, separated by spacer sequences of 587 bp and 436 bp, respectively. The mouse tRNA gene cluster is homologous to a rat sequence (Sekiya et al...

journal_title：Nucleic acids research

authors： Looney JE,Harding JD

更新日期：1983-12-20 00:00:00

abstract：:UHRF1 plays multiple roles in regulating DNMT1-mediated DNA methylation maintenance during DNA replication. The UHRF1 C-terminal RING finger functions as an ubiquitin E3 ligase to establish histone H3 ubiquitination at Lys18 and/or Lys23, which is subsequently recognized by DNMT1 to promote its localization onto repli...

journal_title：Nucleic acids research

authors： Li T,Wang L,Du Y,Xie S,Yang X,Lian F,Zhou Z,Qian C

更新日期：2018-04-06 00:00:00

abstract：:The genetic etiology of premature ovarian insufficiency (POI) has been well established to date, however, the role of long noncoding RNAs (lncRNAs) in POI is largely unknown. In this study, we identified a down-expressed lncRNA HCP5 in granulosa cells (GCs) from biochemical POI (bPOI) patients, which impaired DNA dama...

journal_title：Nucleic acids research

authors： Wang X,Zhang X,Dang Y,Li D,Lu G,Chan WY,Leung PCK,Zhao S,Qin Y,Chen ZJ

更新日期：2020-05-07 00:00:00

abstract：:In squid (Loligo bleekeri) mitochondria, the two 3'-terminal nucleotides (G72-G73) of the tRNA(Tyr) gene overlap with the two 5'-terminal nucleotides (G1-G2) of the downstream tRNA(Cys) gene. To elucidate the processing mechanism(s) of the tRNA molecules derived from this region, tRNAs were analyzed by sequencing cDNA...

journal_title：Nucleic acids research

authors： Tomita K,Ueda T,Watanabe K

更新日期：1996-12-15 00:00:00

abstract：:In order to examine the nature of the complex formation between the ribosomal protein S1 and nucleic acids three methods were used: Inhibition of the reaction of n-ethyl[2.3 14C]-maleimide with S1 by the addition of oligonucleotides; adsorption of the complexes to nitrocellulose filters; and equilibrium dialysis. The ...

journal_title：Nucleic acids research

authors： Lipecky R,Kohlschein J,Gassen HG

更新日期：1977-10-01 00:00:00

abstract：:A flow-injection (FI) device is combined, through the use of a low-volume (4 microl) flow cell, with an ultrasensitive surface plasmon resonance (SPR) spectrometer equipped with a bi-cell photodiode detector. The application of this novel FI-SPR device for sequence-specific ultratrace analysis of oligodeoxynucleotides...

journal_title：Nucleic acids research

authors： Song F,Zhou F,Wang J,Tao N,Lin J,Vellanoweth RL,Morquecho Y,Wheeler-Laidman J

更新日期：2002-07-15 00:00:00

abstract：:WebProAnalyst is a web-accessible analysis tool (http://wwwmgs.bionet.nsc.ru/mgs/programs/panalyst/) designed for scanning quantitative structure-activity relationships in protein families. The tool allows users to search correlations between protein activity and physicochemical characteristics (i.e. hydrophobicity or...

journal_title：Nucleic acids research

authors： Ivanisenko VA,Eroshkin AM,Kolchanov NA

更新日期：2005-07-01 00:00:00

abstract：:Endonuclease III from Escherichia coli, yeast (yNtg1p and yNtg2p) and human and E.coli endonuclease VIII have a wide substrate specificity, and recognize oxidation products of both thymine and cytosine. DNA containing single dihydrouracil (DHU) and tandem DHU lesions were used as substrates for these repair enzymes. I...

journal_title：Nucleic acids research

authors： Venkhataraman R,Donald CD,Roy R,You HJ,Doetsch PW,Kow YW

更新日期：2001-01-15 00:00:00

abstract：:Initiation of sporulation in Bacillus subtilis is controlled by several regulators which affect activation by phosphorylation of the key response regulator Spo0A or transcription of Spo0A-P-dependent genes. In vivo overexpression of one of these regulators, sinR , results in suppression of transcription from the Spo0A...

journal_title：Nucleic acids research

authors： Cervin MA,Lewis RJ,Brannigan JA,Spiegelman GB

更新日期：1998-08-15 00:00:00

abstract：:The nature of mammalian origins of DNA replication remains controversial and this is primarily because two-dimensional gel replicon mapping techniques have identified broad zones of replication initiation whereas several other techniques, such as quantitative PCR, have disclosed more discrete sites of initiation at th...

journal_title：Nucleic acids research

authors： Toledo F,Baron B,Fernandez MA,Lachagès AM,Mayau V,Buttin G,Debatisse M

更新日期：1998-05-15 00:00:00

abstract：:Histone H2B O-GlcNAcylation is an important post-translational modification of chromatin during gene transcription. However, how this epigenetic modification is regulated remains unclear. Here we found that the energy-sensing adenosine-monophosphate-activated protein kinase (AMPK) could suppress histone H2B O-GlcNAcyl...

journal_title：Nucleic acids research

authors： Xu Q,Yang C,Du Y,Chen Y,Liu H,Deng M,Zhang H,Zhang L,Liu T,Liu Q,Wang L,Lou Z,Pei H

更新日期：2014-05-01 00:00:00

abstract：:The Cox protein from bacteriophage P2 forms oligomeric filaments and it has been proposed that DNA can be wound up around these filaments, similar to how histones condense DNA. We here use fluorescence microscopy to study single DNA-Cox complexes in nanofluidic channels and compare how the Cox homologs from phages P2 ...

journal_title：Nucleic acids research

authors： Frykholm K,Berntsson RP,Claesson M,de Battice L,Odegrip R,Stenmark P,Westerlund F

更新日期：2016-09-06 00:00:00

abstract：:Upon infection of Escherichia coli by bacteriophage Qβ, the virus-encoded β-subunit recruits host translation elongation factors EF-Tu and EF-Ts and ribosomal protein S1 to form the Qβ replicase holoenzyme complex, which is responsible for amplifying the Qβ (+)-RNA genome. Here, we use X-ray crystallography, NMR spect...

journal_title：Nucleic acids research

authors： Gytz H,Mohr D,Seweryn P,Yoshimura Y,Kutlubaeva Z,Dolman F,Chelchessa B,Chetverin AB,Mulder FA,Brodersen DE,Knudsen CR

更新日期：2015-12-15 00:00:00

abstract：:The continuing threat of infectious disease and future pandemics, coupled to the continuous increase of drug-resistant pathogens, makes the discovery of new and better vaccines imperative. For effective vaccine development, antigen discovery and validation is a prerequisite. The compilation of information concerning p...

journal_title：Nucleic acids research

authors： Ansari HR,Flower DR,Raghava GP

更新日期：2010-01-01 00:00:00

abstract：:Oligoribonucleotides with 2'-5' linkages have been synthesized on solid support. UV melting and CD experiments indicate complementary strands associate to give complexes with melting temperatures 30 to 40 degrees C lower than for duplexes formed by 3'-5' oligoribonucleotides with the same sequence. UV melting and imin...

journal_title：Nucleic acids research

authors： Kierzek R,He L,Turner DH

更新日期：1992-04-11 00:00:00

abstract：:G-quadruplexes are dynamic structures folded in G-rich single-stranded DNA regions. These structures have been recognized as a potential nucleic acid based mechanism for regulating multiple cellular processes such as replication, transcription and genomic maintenance. So far, their transcriptional role in vivo during ...

journal_title：Nucleic acids research

authors： David AP,Margarit E,Domizi P,Banchio C,Armas P,Calcaterra NB

更新日期：2016-05-19 00:00:00

abstract：:By using a synthetic DNA probe derived from an amino acid sequence in the most conserved region of three known photolyases (Escherichia coli, Anacystis nidulans and Saccharomyces cerevisiae), we isolated a DNA fragment containing two long open reading frames (ORFs) from a genomic DNA library of Streptomyces griseus. O...

journal_title：Nucleic acids research

authors： Kobayashi T,Takao M,Oikawa A,Yasui A

更新日期：1989-06-26 00:00:00

abstract：:Expression from the K. pneumoniae nifLA promoter is oxygen sensitive and is also inhibited by the DNA gyrase inhibitor coumermycin A1 under anaerobic growth conditions. The activity of this promoter was found to be highly sensitive to changes in DNA topology in vitro. Transcription was completely dependent on negative...

journal_title：Nucleic acids research

authors： Dixon RA,Henderson NC,Austin S

更新日期：1988-11-11 00:00:00

abstract：:Poly 2'-azido-2'-deoxyadenylic acid (Poly Az) was synthesized from 2'-azido-2'-deoxyadenosine diphosphate by polynucleotide phosphorylase. Poly (Az) has U.V. absorption properties similar to poly (A) and hypochromicity of 40% at 0.1 M Na+ and neutrality. CD curve also resembled to that of poly (A), but has smaller ell...

journal_title：Nucleic acids research

authors： Ikehara M,Fukui T,Kakiuchi N

更新日期：1976-08-01 00:00:00

abstract：:Most human genes generate multiple transcript isoforms. The differential expression of these isoforms can help specify cell types. Diverse transcript isoforms arise from the use of alternative transcription start sites, polyadenylation sites and splice sites; however, the relative contribution of these processes to is...

journal_title：Nucleic acids research

authors： Reyes A,Huber W

更新日期：2018-01-25 00:00:00

abstract：:An enzyme activity introducing an alkali-labile site at 2-hydroxyadenine (2-OH-A) in double-stranded oligonucleotides was detected in nuclear extracts of Jurkat cells. This activity co-eluted with activities toward adenine paired with guanine and 8-oxo-7,8-dihydroguanine (8-oxoG) as a single peak corresponding to a 55...

journal_title：Nucleic acids research

authors： Ohtsubo T,Nishioka K,Imaiso Y,Iwai S,Shimokawa H,Oda H,Fujiwara T,Nakabeppu Y

更新日期：2000-03-15 00:00:00

abstract：:Egr-1 is a murine zinc finger encoding cDNA whose expression is modulated by a variety of ligand-receptor interactions and is often coregulated with c-fos (1). This study reports the isolation of a mouse Egr-1 genomic clone, its intron-exon structure, and 935 bp of 5' flanking sequence. The gene spans about 3.8 kb and...

journal_title：Nucleic acids research

authors： Tsai-Morris CH,Cao XM,Sukhatme VP

更新日期：1988-09-26 00:00:00

abstract：:The sequence of the 1173 nt 12S kinetoplast ribosomal RNA from Leishmania tarentolae was determined from the maxicircle DNA sequence, and the 5' and 3' ends localized by primer runoff and S1 nuclease protection experiments. The gene was shown to be free of introns by S1 nuclease analysis. A partial secondary structure...

journal_title：Nucleic acids research

authors： de la Cruz VF,Simpson AM,Lake JA,Simpson L

更新日期：1985-04-11 00:00:00

abstract：:Preparations of chicken, rat and human nuclear 5S RNA contain two sets of molecules. The set with the lowest electrophoretic mobility (5Sa) contains RNAs identical or closely related to ribosomal 5S RNA from the corresponding animal species. In HeLa cells and rat brain, we only detected an RNA identical to the ribosom...

journal_title：Nucleic acids research

authors： Krol A,Gallinaro H,Lazar E,Jacob M,Branlant C

更新日期：1981-02-25 00:00:00

abstract：:The Neurospora VS ribozyme is a catalytic RNA that has the unique ability to specifically recognize and cleave a stem-loop substrate through formation of a highly stable kissing-loop interaction (KLI). In order to explore the engineering potential of the VS ribozyme to cleave alternate substrates, we substituted the w...

journal_title：Nucleic acids research

authors： Lacroix-Labonté J,Girard N,Dagenais P,Legault P

更新日期：2016-08-19 00:00:00

abstract：:Eight base analogs were tested as third strand residues in otherwise homopyrimidine strands opposite each of the 'direct' (A.T and G.C) and 'inverted' (T.A and C.G) Watson-Crick base pairs, using UV melting profiles to assess triplex stability. The target duplexes contained 20 A.T base pairs and a central test base pa...

journal_title：Nucleic acids research

authors： Amosova OA,Fresco JR

更新日期：1999-12-01 00:00:00

abstract：:A RNA interference (RNAi) like mechanism is involved in elimination of thousands of DNA segments from the developing somatic macronucleus of Tetrahymena, yet how specific internal eliminated sequences (IESs) are recognized remains to be fully elucidated. To define requirements for DNA rearrangement, we performed mutag...

journal_title：Nucleic acids research

authors： Kowalczyk CA,Anderson AM,Arce-Larreta M,Chalker DL

更新日期：2006-01-01 00:00:00

abstract：:The Central glycolytic genes Repressor (CggR) from Bacillus subtilis belongs to the SorC family of transcription factors that control major carbohydrate metabolic pathways. Recent studies have shown that CggR binds as a tetramer to its tandem operator DNA sequences and that the inducer metabolite, fructose 1,6-bisphos...

journal_title：Nucleic acids research

authors： Chaix D,Ferguson ML,Atmanene C,Van Dorsselaer A,Sanglier-Cianférani S,Royer CA,Declerck N

更新日期：2010-09-01 00:00:00

abstract：:Many infectious diseases are caused by viral infections, and in particular by RNA viruses such as MERS, Ebola and Zika. To understand viral disease, detection and identification of these viruses are essential. Although PCR is widely used for rapid virus identification due to its low cost and high sensitivity and speci...

journal_title：Nucleic acids research

authors： Kim H,Kang N,An K,Kim D,Koo J,Kim MS

更新日期：2017-01-04 00:00:00

abstract：:In a pilot study on SAGE on Reed-Sternberg cells we have sequenced 1055 tags representing 701 genes. Screening of the GenBank database resulted in the identification of a corresponding gene or EST for 490 of them. For 211 of the tags no homology could be detected. A major problem of the serial analysis of gene express...

journal_title：Nucleic acids research

authors： van den Berg A,van der Leij J,Poppema S

更新日期：1999-09-01 00:00:00