当前位置： SCI文献检索 > NUCLEIC ACIDS RESEARCH期刊下所有文献 > Complex formation between ribosomal protein S1, oligo-and polynucleotides: chain length dependence and base specificity.

Complex formation between ribosomal protein S1, oligo-and polynucleotides: chain length dependence and base specificity.

Abstract：

:In order to examine the nature of the complex formation between the ribosomal protein S1 and nucleic acids three methods were used: Inhibition of the reaction of n-ethyl[2.3 14C]-maleimide with S1 by the addition of oligonucleotides; adsorption of the complexes to nitrocellulose filters; and equilibrium dialysis. The complex formation is Mg2+ dependent at low salt concentrations and becomes Mg2+ independent at an ionic strength greater than 90 mM. Oligouridylates of increasing chain length reach an optimal KA of 3-3-10(7) M-1 at a chain length of n=13-14. Protein S1 contains one binding site for long chain oligouridylates, such as U12, and the standard-free-energy change on binding caused by one Pu increment is 0.41 kcal/mol, when n varies between five and fourteen. Complex formation is insensitive to the capacity of the homopolynucleotide bases to form hydrogen bonds. Homopolynuceotides, however, showing a Tm less than 250 in the buffer system used show an increased affinity for S1 compared to poly(A) and poly(C) (Tm greater than 40 degrees). The data are discussed with respect to the proposed binding of protein S1 to the 3-terminal end of the 16S RNA.

journal_name

Nucleic Acids Res

journal_title

Nucleic acids research

authors

Lipecky R,Kohlschein J,Gassen HG

doi

10.1093/nar/4.10.3627

subject

Has Abstract

pub_date

1977-10-01 00:00:00

pages

3627-42

issue

10

eissn

0305-1048

issn

1362-4962

journal_volume

4

pub_type

杂志文章

相关文献

NUCLEIC ACIDS RESEARCH文献大全
  • Genomic shotgun array: a procedure linking large-scale DNA sequencing with regional transcript mapping.

    abstract：:To facilitate transcript mapping and to investigate alterations in genomic structure and gene expression in a defined genomic target, we developed a novel microarray-based method to detect transcriptional activity of the human chromosome 4q22-24 region. Loss of heterozygosity of human 4q22-24 is frequently observed in...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gnh025

    authors： Li LH,Li JC,Lin YF,Lin CY,Chen CY,Tsai SF

    更新日期：2004-02-11 00:00:00

  • Yeast killer dsRNA plasmids are transcribed in vivo to produce full and partial-length plus-stranded RNAs.

    abstract：:In vivo transcripts of the L (4.5 kb) and M (1.9 kb) dsRNA plasmids were examined in type I killers of Saccharomyces cerevisiae. Transcripts for both plasmids include full-length (l,m) and partial-length (la,ma) single-stranded species. Both L-dsRNA transcripts (l,la) have in vitro mRNA activity for L-P1, previously s...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/11.4.1077

    authors： Bostian KA,Burn VE,Jayachandran S,Tipper DJ

    更新日期：1983-02-25 00:00:00

  • G-quadruplexes within prion mRNA: the missing link in prion disease?

    abstract：:Cellular ribonucleic acid (RNA) plays a crucial role in the initial conversion of cellular prion protein PrP(C) to infectious PrP(Sc) or scrapie. The nature of this RNA remains elusive. Previously, RNA aptamers against PrP(C) have been isolated and found to form G-quadruplexes (G4s). PrP(C) binding to G4 RNAs destabil...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gku559

    authors： Olsthoorn RC

    更新日期：2014-08-01 00:00:00

  • BioModels Database: a free, centralized database of curated, published, quantitative kinetic models of biochemical and cellular systems.

    abstract：:BioModels Database (http://www.ebi.ac.uk/biomodels/), part of the international initiative BioModels.net, provides access to published, peer-reviewed, quantitative models of biochemical and cellular systems. Each model is carefully curated to verify that it corresponds to the reference publication and gives the proper...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkj092

    authors： Le Novère N,Bornstein B,Broicher A,Courtot M,Donizelli M,Dharuri H,Li L,Sauro H,Schilstra M,Shapiro B,Snoep JL,Hucka M

    更新日期：2006-01-01 00:00:00

  • Spatial organization of topoisomerase I-mediated DNA cleavage induced by camptothecin-oligonucleotide conjugates.

    abstract：:Triple helix-forming oligonucleotides covalently linked to topoisomerase I inhibitors, in particular the antitumor agent camptothecin, trigger topoisomerase I-mediated DNA cleavage selectively in the proximity of the binding site of the oligonucleotide vector. In the present study, we have performed a systematic analy...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkg457

    authors： Arimondo PB,Angenault S,Halby L,Boutorine A,Schmidt F,Monneret C,Garestier T,Sun JS,Bailly C,Hélène C

    更新日期：2003-07-15 00:00:00

  • A compact form of double-stranded RNA in solutions containing poly(ethyleneglycol).

    abstract：:Molecules of single-stranded ribosomal RNA and double-stranded replicative form of phage f2 RNA (dsRNA) adopt a compact form in solutions, containing sufficiently high concentrations of salt (NaCl) and polymer (PEG). However, only in the cases of native dsRNA molecules the compact particles are characterized by a regu...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/3.6.1533

    authors： Evdokimov YM,Pyatigorskaya TL,Kadikov VA,Polyvtsev OF,Doskocil J,Koudelka J,Varshavsky YM

    更新日期：1976-06-01 00:00:00

  • Selection of sequence elements that substitute for the standard AATAAA motif which signals 3' processing and polyadenylation of late simian virus 40 mRNAs.

    abstract：:A method is described which allows selection of sequences which can substitute for the normal AATAAA hexanucleotide involved in polyadenylation of SV40 late mRNAs. Plaques were generated from viral DNA lacking the motif, forcing acquisition of substitute sequences. Four variants were characterized. All displayed wild-...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/13.22.8053

    authors： Swimmer C,Shenk T

    更新日期：1985-11-25 00:00:00

  • EGF-induced jun B-expression in transfected P19 embryonal carcinoma cells expressing EGF-receptors is dependent on Jun D.

    abstract：:The TPA-inducible transcription factor AP-1, consisting of homo- or hetero-dimers of members of the Jun- and Fos-families, regulates transcription of a wide variety of genes containing the TPA response element (TRE). In P19 embryonal carcinoma (EC) cells, Jun D is the only component of AP-1 expressed, while in these c...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/20.1.125

    authors： den Hertog J,de Groot RP,de Laat SW,Kruijer W

    更新日期：1992-01-11 00:00:00

  • Circularized synthetic oligodeoxynucleotides serve as promoterless RNA polymerase III templates for small RNA generation in human cells.

    abstract：:Synthetic RNA formulations and viral vectors are the two main approaches for delivering small therapeutic RNA to human cells. Here we report findings supporting an alternative strategy in which an endogenous human RNA polymerase (RNAP) is harnessed to make RNA hairpin-containing small RNA from synthetic single-strande...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gks1334

    authors： Seidl CI,Lama L,Ryan K

    更新日期：2013-02-01 00:00:00

  • Oryza Tag Line, a phenotypic mutant database for the Genoplante rice insertion line library.

    abstract：:To organize data resulting from the phenotypic characterization of a library of 30,000 T-DNA enhancer trap (ET) insertion lines of rice (Oryza sativa L cv. Nipponbare), we developed the Oryza Tag Line (OTL) database (http://urgi.versailles.inra.fr/OryzaTagLine/). OTL structure facilitates forward genetic search for sp...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkm762

    authors： Larmande P,Gay C,Lorieux M,Périn C,Bouniol M,Droc G,Sallaud C,Perez P,Barnola I,Biderre-Petit C,Martin J,Morel JB,Johnson AA,Bourgis F,Ghesquière A,Ruiz M,Courtois B,Guiderdoni E

    更新日期：2008-01-01 00:00:00

  • Master regulatory GATA transcription factors: mechanistic principles and emerging links to hematologic malignancies.

    abstract：:Numerous examples exist of how disrupting the actions of physiological regulators of blood cell development yields hematologic malignancies. The master regulator of hematopoietic stem/progenitor cells GATA-2 was cloned almost 20 years ago, and elegant genetic analyses demonstrated its essential function to promote hem...

    journal_title：Nucleic acids research

    pub_type： 杂志文章,评审

    doi：10.1093/nar/gks281

    authors： Bresnick EH,Katsumura KR,Lee HY,Johnson KD,Perkins AS

    更新日期：2012-07-01 00:00:00

  • Induced DNA demethylation by targeting Ten-Eleven Translocation 2 to the human ICAM-1 promoter.

    abstract：:Increasing evidence indicates that active DNA demethylation is involved in several processes in mammals, resulting in developmental stage-specificity and cell lineage-specificity. The recently discovered Ten-Eleven Translocation (TET) dioxygenases are accepted to be involved in DNA demethylation by initiating 5-mC oxi...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkt1019

    authors： Chen H,Kazemier HG,de Groote ML,Ruiters MH,Xu GL,Rots MG

    更新日期：2014-02-01 00:00:00

  • A correlation with exon expression approach to identify cis-regulatory elements for tissue-specific alternative splicing.

    abstract：:Correlation of motif occurrences with gene expression intensity is an effective strategy for elucidating transcriptional cis-regulatory logic. Here we demonstrate that this approach can also identify cis-regulatory elements for alternative pre-mRNA splicing. Using data from a human exon microarray, we identified 56 ca...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkm485

    authors： Das D,Clark TA,Schweitzer A,Yamamoto M,Marr H,Arribere J,Minovitsky S,Poliakov A,Dubchak I,Blume JE,Conboy JG

    更新日期：2007-01-01 00:00:00

  • Phospho.ELM: a database of phosphorylation sites--update 2011.

    abstract：:The Phospho.ELM resource (http://phospho.elm.eu.org) is a relational database designed to store in vivo and in vitro phosphorylation data extracted from the scientific literature and phosphoproteomic analyses. The resource has been actively developed for more than 7 years and currently comprises 42,574 serine, threoni...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkq1104

    authors： Dinkel H,Chica C,Via A,Gould CM,Jensen LJ,Gibson TJ,Diella F

    更新日期：2011-01-01 00:00:00

  • Overexpression of eIF5 or its protein mimic 5MP perturbs eIF2 function and induces ATF4 translation through delayed re-initiation.

    abstract：:ATF4 is a pro-oncogenic transcription factor whose translation is activated by eIF2 phosphorylation through delayed re-initiation involving two uORFs in the mRNA leader. However, in yeast, the effect of eIF2 phosphorylation can be mimicked by eIF5 overexpression, which turns eIF5 into translational inhibitor, thereby ...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkw559

    authors： Kozel C,Thompson B,Hustak S,Moore C,Nakashima A,Singh CR,Reid M,Cox C,Papadopoulos E,Luna RE,Anderson A,Tagami H,Hiraishi H,Slone EA,Yoshino KI,Asano M,Gillaspie S,Nietfeld J,Perchellet JP,Rothenburg S,Masai H,W

    更新日期：2016-10-14 00:00:00

  • Complete nucleotide sequence of the 23S rRNA gene of the Cyanobacterium, Anacystis nidulans.

    abstract：:The nucleotide sequence of the Anacystis nidulans 23S rRNA gene, including the 5'- and 3'-flanking regions has been determined. The gene is 2876 nucleotides long and shows higher primary sequence homology to the 23S rRNAs of plastids (84.5%) than to that of E. coli (79%). The predicted rRNA transcript also shares many...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/12.7.3373

    authors： Douglas SE,Doolittle WF

    更新日期：1984-04-11 00:00:00

  • An interplay of the base excision repair and mismatch repair pathways in active DNA demethylation.

    abstract：:Active DNA demethylation (ADDM) in mammals occurs via hydroxylation of 5-methylcytosine (5mC) by TET and/or deamination by AID/APOBEC family enzymes. The resulting 5mC derivatives are removed through the base excision repair (BER) pathway. At present, it is unclear how the cell manages to eliminate closely spaced 5mC ...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkw059

    authors： Grin I,Ishchenko AA

    更新日期：2016-05-05 00:00:00

  • Mobile dispersed genetic element MDG1 of Drosophila melanogaster: transcription pattern.

    abstract：:Virtually the whole region of the mobile dispersed genetic element mdg1 including the direct repeats that frame mdg1 /1/ is transcribed. The end of the transcriptional unit is located within the direct repeat. Although the transcription of mdg1 is symmetric, one direction is transcribed preferentially and is a full-le...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/8.22.5347

    authors： Ilyin Y,Chmeliauskaite VG,Kulguskin VV,Georgiev GP

    更新日期：1980-11-25 00:00:00

  • Characterization of the 3' exonuclease subunit DP1 of Methanococcus jannaschii replicative DNA polymerase D.

    abstract：:The B-subunits associated with the replicative DNA polymerases are conserved from Archaea to humans, whereas the corresponding catalytic subunits are not related. The latter belong to the B and D DNA polymerase families in eukaryotes and archaea, respectively. Sequence analysis places the B-subunits within the calcine...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkh558

    authors： Jokela M,Eskelinen A,Pospiech H,Rouvinen J,Syväoja JE

    更新日期：2004-04-30 00:00:00

  • LATERAL ORGAN BOUNDARIES defines a new family of DNA-binding transcription factors and can interact with specific bHLH proteins.

    abstract：:Conserved in a variety of evolutionarily divergent plant species, LOB DOMAIN (LBD) genes define a large, plant-specific family of largely unknown function. LBD genes have been implicated in a variety of developmental processes in plants, although to date, relatively few members have been assigned functions. LBD protei...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkm775

    authors： Husbands A,Bell EM,Shuai B,Smith HM,Springer PS

    更新日期：2007-01-01 00:00:00

  • Binding site requirements and differential representation of TGF factors in nuclear ASF-1 activity.

    abstract：:Activating sequence factor 1 (ASF-1) is a nuclear DNA-binding activity that is found in monocots and dicots. It interacts with several TGACG-containing elements that have been characterized from viral and T-DNA genes, the prototypes of which are the as-1 element of the CaMV 35S promoter and the ocs element from the oc...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/23.18.3778

    authors： Lam E,Lam YK

    更新日期：1995-09-25 00:00:00

  • alpha-DNA. I. Synthesis, characterization by high field 1H-NMR, and base-pairing properties of the unnatural hexadeoxyribonucleotide alpha-[d(CpCpTpTpCpC)] with its complement beta-[d(GpGpApApGpG)].

    abstract：:The novel deoxyribonucleotide alpha-[d(CpCpTpTpCpC)] and its complement beta-[d(GpGpApApGpG)] were synthesized by the phosphotriester method. 1H-NMR-NOE examination of the alpha-hexamer revealed that the cytosine and thymine bases appear to adopt anti conformations in this strand. In addition the deoxyribose of the th...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/14.12.5019

    authors： Morvan F,Rayner B,Imbach JL,Chang DK,Lown JW

    更新日期：1986-06-25 00:00:00

  • Gene integrated set profile analysis: a context-based approach for inferring biological endpoints.

    abstract：:The identification of genes with specific patterns of change (e.g. down-regulated and methylated) as phenotype drivers or samples with similar profiles for a given gene set as drivers of clinical outcome, requires the integration of several genomic data types for which an 'integrate by intersection' (IBI) approach is ...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkv1503

    authors： Kowalski J,Dwivedi B,Newman S,Switchenko JM,Pauly R,Gutman DA,Arora J,Gandhi K,Ainslie K,Doho G,Qin Z,Moreno CS,Rossi MR,Vertino PM,Lonial S,Bernal-Mizrachi L,Boise LH

    更新日期：2016-04-20 00:00:00

  • ADAR2 A-->I editing: site selectivity and editing efficiency are separate events.

    abstract：:ADAR enzymes, adenosine deaminases that act on RNA, form a family of RNA editing enzymes that convert adenosine to inosine within RNA that is completely or largely double-stranded. Site-selective A-->I editing has been detected at specific sites within a few structured pre-mRNAs of metazoans. We have analyzed the edit...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkg681

    authors： Källman AM,Sahlin M,Ohman M

    更新日期：2003-08-15 00:00:00

  • Murine Stat2 is uncharacteristically divergent.

    abstract：:Characterization of the ability of human interferons (IFNs) to rapidly induce genes led to the identification of the first two members of the STAT (signal transducers and activators of transcription) family, Stat1 and Stat2. To study the unique role of this transcription factor in IFN signaling under more physiologica...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/27.21.4191

    authors： Park C,Lecomte MJ,Schindler C

    更新日期：1999-11-01 00:00:00

  • Tetrahymena Genome Database (TGD): a new genomic resource for Tetrahymena thermophila research.

    abstract：:We have developed a web-based resource (available at www.ciliate.org) for researchers studying the model ciliate organism Tetrahymena thermophila. Employing the underlying database structure and programming of the Saccharomyces Genome Database, the Tetrahymena Genome Database (TGD) integrates the wealth of knowledge g...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkj054

    authors： Stover NA,Krieger CJ,Binkley G,Dong Q,Fisk DG,Nash R,Sethuraman A,Weng S,Cherry JM

    更新日期：2006-01-01 00:00:00

  • The DNA sequence at the T7 C promoter.

    abstract：:Restriction fragments of T7 DNA which selectively bind E. coli RNA polymerase have been identified. These include fragments located close to the beginning of gene 1 where according to Minkley and Pribnow (1973) there is a promoter called C. The smallest fragment from this region which binds RNA polymerase has been seq...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/6.2.525

    authors： McConnell DJ

    更新日期：1979-02-01 00:00:00

  • Composition and arrangement of genes define the strength of IRES-driven translation in bicistronic mRNAs.

    abstract：:In addition to the cap-dependent mechanism, eukaryotic initiation of translation can occur by a cap-independent mechanism which directs ribosomes to defined start codons enabled by internal ribosome entry site (IRES) elements. IRES elements from poliovirus and encephalomyocarditis virus are often used to construct bi-...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/29.16.3327

    authors： Hennecke M,Kwissa M,Metzger K,Oumard A,Kröger A,Schirmbeck R,Reimann J,Hauser H

    更新日期：2001-08-15 00:00:00

  • DNA binding modes influence Rap1 activity in the regulation of telomere length and MRX functions at DNA ends.

    abstract：:The cellular response to DNA double-strand breaks (DSBs) is initiated by the Mre11-Rad50-Xrs2 (MRX) complex that has structural and catalytic functions. MRX association at DSBs is counteracted by Rif2, which is known to interact with Rap1 that binds telomeric DNA through two tandem Myb-like domains. Whether and how Ra...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkz1203

    authors： Bonetti D,Rinaldi C,Vertemara J,Notaro M,Pizzul P,Tisi R,Zampella G,Longhese MP

    更新日期：2020-03-18 00:00:00

  • Common TFIIH recruitment mechanism in global genome and transcription-coupled repair subpathways.

    abstract：:Nucleotide excision repair is initiated by two different damage recognition subpathways, global genome repair (GGR) and transcription-coupled repair (TCR). In GGR, XPC detects DNA lesions and recruits TFIIH via interaction with the pleckstrin homology (PH) domain of TFIIH subunit p62. In TCR, an elongating form of RNA...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkx970

    authors： Okuda M,Nakazawa Y,Guo C,Ogi T,Nishimura Y

    更新日期：2017-12-15 00:00:00