An interplay of the base excision repair and mismatch repair pathways in active DNA demethylation.

abstract：:The study of pathogenic mitochondrial DNA mutations has, in most cases, relied on the production of transmitochondrial cybrids. Although the procedure to produce such cybrids is well established, it is laborious and cumbersome. Moreover, the mechanical enucleation procedure is inefficient and different techniques have...

journal_title：Nucleic acids research

authors： Bayona-Bafaluy MP,Manfredi G,Moraes CT

更新日期：2003-08-15 00:00:00

abstract：:The TGGCA protein, the chicken homologue of HeLa cell NF-I, was purified to homogeneity from liver tissue by a procedure which includes preparative mobility shift electrophoresis (PMSE) as the final step. PMSE was here adjusted for the isolation of the TGGCA protein, but can be used as a general method to characterize...

journal_title：Nucleic acids research

authors： Rupp RA,Sippel AE

更新日期：1987-12-10 00:00:00

abstract：:The KEGG RPAIR database is a collection of biochemical structure transformation patterns, called RDM patterns, and chemical structure alignments of substrate-product pairs (reactant pairs) in all known enzyme-catalyzed reactions taken from the Enzyme Nomenclature and the KEGG PATHWAY database. Here, we present PathPre...

journal_title：Nucleic acids research

authors： Moriya Y,Shigemizu D,Hattori M,Tokimatsu T,Kotera M,Goto S,Kanehisa M

更新日期：2010-07-01 00:00:00

abstract：:We have constructed vectors from bacteriophage lambda and from plasmid pBR322 having a single EcoRI restriction site which is immediately downstream from the lac UV5 promotor. Each vector allows the fusion of a cloned gene to the lac Z gene in a different phase relative to the translation initiation codon of the lac Z...

journal_title：Nucleic acids research

authors： Charnay P,Perricaudet M,Galibert F,Tiollais P

更新日期：1978-12-01 00:00:00

abstract：:By transporting one DNA double helix (T-segment) through a double-strand break in another (G-segment), topoisomerase II reduces fractions of DNA catenanes, knots and supercoils to below equilibrium values. How DNA segments are selected to simplify the equilibrium DNA topology is enigmatic, and the biological relevance...

journal_title：Nucleic acids research

authors： Martínez-García B,Fernández X,Díaz-Ingelmo O,Rodríguez-Campos A,Manichanh C,Roca J

更新日期：2014-02-01 00:00:00

abstract：:Two new beta-lactoglobulin (BLG)/human serum albumin (HSA) hybrid gene vectors were constructed and tested for expression in COS-7 cells and in transgenic mice. The HSA sequences were inserted between the second and sixth BLG exons. Transient transfection experiments with these vectors as well as a series of additiona...

journal_title：Nucleic acids research

authors： Barash I,Nathan M,Kari R,Ilan N,Shani M,Hurwitz DR

更新日期：1996-02-15 00:00:00

abstract：:Nucleotide excision DNA repair is mechanistically conserved across all kingdoms of life. In prokaryotes, this multi-enzyme process requires six proteins: UvrA-D, DNA polymerase I and DNA ligase. To examine how UvrC locates the UvrB-DNA pre-incision complex at a site of damage, we have labeled UvrB and UvrC with differ...

journal_title：Nucleic acids research

authors： Hughes CD,Wang H,Ghodke H,Simons M,Towheed A,Peng Y,Van Houten B,Kad NM

更新日期：2013-05-01 00:00:00

abstract：:Carbohydrate-binding proteins play crucial roles across all organisms and viruses. The complexity of carbohydrate structures, together with inconsistencies in how their 3D structures are reported, has led to difficulties in characterizing the protein-carbohydrate interfaces. In order to better understand protein-carbo...

journal_title：Nucleic acids research

authors： Copoiu L,Torres PHM,Ascher DB,Blundell TL,Malhotra S

更新日期：2020-01-08 00:00:00

abstract：:Protein species interacting with newly replicated DNA were analyzed using a photo cross-linking technique. Nascent DNA was labeled in vitro with [alpha-32P]dCTP and BrdUTP in SV40-infected CV-1 cells made permeable with saponin. The labeled cells were then irradiated with UV light (254 nm) and were treated extensively...

journal_title：Nucleic acids research

authors： Tsutsui K,Watanabe S,Katagiri M,Oda T

更新日期：1983-07-25 00:00:00

abstract：:Controller (C) proteins regulate the timing of the expression of restriction and modification (R-M) genes through a combination of positive and negative feedback circuits. A single dimer bound to the operator switches on transcription of the C-gene and the endonuclease gene; at higher concentrations, a second dimer bo...

journal_title：Nucleic acids research

authors： McGeehan JE,Streeter SD,Thresh SJ,Ball N,Ravelli RB,Kneale GG

更新日期：2008-08-01 00:00:00

abstract：:The endonuclease activity within the influenza virus cap-snatching process is a proven therapeutic target. The anti-influenza drug baloxavir is highly effective, but is associated with resistance mutations that threaten its clinical efficacy. The endonuclease resides within the N-terminal domain of the PA subunit (PAN...

journal_title：Nucleic acids research

authors： Kumar G,Cuypers M,Webby RR,Webb TR,White SW

更新日期：2021-01-19 00:00:00

abstract：:Cellular senescence, an integral component of aging and cancer, arises in response to diverse triggers, including telomere attrition, macromolecular damage and signaling from activated oncogenes. At present, senescent cells are identified by the combined presence of multiple traits, such as senescence-associated prote...

journal_title：Nucleic acids research

authors： Casella G,Munk R,Kim KM,Piao Y,De S,Abdelmohsen K,Gorospe M

更新日期：2019-08-22 00:00:00

abstract：:The coiled-coil coactivator (CoCoA) is a transcriptional coactivator for nuclear receptors and enhances nuclear receptor function by the interaction with the bHLH-PAS domain (AD3) of p160 coactivators. The C-terminal activation domain (AD) of CoCoA possesses strong transactivation activity and is required for the coac...

journal_title：Nucleic acids research

authors： Kim JH,Yang CK,Stallcup MR

更新日期：2006-05-22 00:00:00

abstract：:An intronic hexanucleotide UGCAUG has been shown to play a critical role in the regulation of tissue-specific alternative splicing of pre-mRNAs in a wide range of tissues. Vertebrate Fox-1 has been shown to bind to this element, in a highly sequence-specific manner, through its RNA recognition motif (RRM). In mammals,...

journal_title：Nucleic acids research

authors： Nakahata S,Kawamoto S

更新日期：2005-04-11 00:00:00

abstract：:Exonic splicing enhancer (ESE) sequences are bound by serine & arginine-rich (SR) proteins, which in turn enhance the recruitment of splicing factors. It was inferred from measurements of splicing around twenty years ago that Drosophila doublesex ESEs are bound stably by SR proteins, and that the bound proteins intera...

journal_title：Nucleic acids research

authors： Jobbins AM,Reichenbach LF,Lucas CM,Hudson AJ,Burley GA,Eperon IC

更新日期：2018-03-16 00:00:00

abstract：:DNA and RNA guanine-quadruplexes (G4s) are stabilized by several cations, in particular by potassium and sodium ions. Generally, potassium stabilizes guanine-quartet assemblies to a larger extent than sodium; in this article we report about a higher-order G4 structure more stable in sodium than in potassium. Repeats o...

journal_title：Nucleic acids research

authors： Saintomé C,Amrane S,Mergny JL,Alberti P

更新日期：2016-04-07 00:00:00

abstract：:Microarrays are an effective tool for monitoring genome-wide gene expression levels. In current microarray analyses, the majority of genes on arrays are frequently eliminated for further analysis because the changes in their expression levels (ratios) are considered to be not significant. This strategy risks failure t...

journal_title：Nucleic acids research

authors： Yano K,Imai K,Shimizu A,Hanashita T

更新日期：2006-03-14 00:00:00

abstract：:We examined the ability of the 5' flanking region sequences of a human U1 RNA gene to direct synthesis of functional mRNA. When fused to chloramphenicol acetyltransferase (CAT) coding region sequences, the upstream sequences of the U1 gene were able to stimulate the synthesis of functional CAT mRNA in 293 cells but no...

journal_title：Nucleic acids research

authors： Dahlberg JE,Schenborn ET

更新日期：1988-07-11 00:00:00

abstract：:The miR-290 cluster is expressed in embryonic stem cells (ESCs) and is important for the maintenance of pluripotency, but little is known about the mechanisms regulating the early embryonic microRNA cluster (EEmiRC) expression. Here we report the identification of a 332-bp intragenic enhancer (IE) able to modulate the...

journal_title：Nucleic acids research

authors： Tata PR,Tata NR,Kühl M,Sirbu IO

更新日期：2011-05-01 00:00:00

abstract：:DNA primer sets, labeled with two fluorescent dyes to exploit fluorescence energy transfer (ET), can be efficiently excited with a single laser line and emit strong fluorescence at distinctive wavelengths. Such ET primers are superior to single fluorophore-labeled primers for DNA sequencing and other multiple color-ba...

journal_title：Nucleic acids research

authors： Ju J,Glazer AN,Mathies RA

更新日期：1996-03-15 00:00:00

abstract：:The bHLH transcription factor MyoD, the prototypical master regulator of differentiation, directs a complex program of gene expression during skeletal myogenesis. The up-regulation of the cdk inhibitor p57kip2 plays a critical role in coordinating differentiation and growth arrest during muscle development, as well as...

journal_title：Nucleic acids research

authors： Busanello A,Battistelli C,Carbone M,Mostocotto C,Maione R

更新日期：2012-09-01 00:00:00

abstract：:Ornithine decarboxylase (ODC) expression is important for proliferation and is elevated in many tumor cells. We previously showed that Sp1 is a major positive regulator of ODC transcription. In this paper we have investigated transcriptional regulation of rat ODC by the closely related factor Sp3. While over-expressio...

journal_title：Nucleic acids research

authors： Kumar AP,Butler AP

更新日期：1997-05-15 00:00:00

abstract：:The structure of a ribonuclease III processing signal from bacteriophage T7 was examined by NMR spectroscopy, optical melting, and chemical and enzymatic modification. A 41 nucleotide variant of the T7 R1.1 processing signal has two Watson-Crick base-paired helices separated by an internal loop, consistent with its pr...

journal_title：Nucleic acids research

authors： Schweisguth DC,Chelladurai BS,Nicholson AW,Moore PB

更新日期：1994-02-25 00:00:00

abstract：:Histone H2B O-GlcNAcylation is an important post-translational modification of chromatin during gene transcription. However, how this epigenetic modification is regulated remains unclear. Here we found that the energy-sensing adenosine-monophosphate-activated protein kinase (AMPK) could suppress histone H2B O-GlcNAcyl...

journal_title：Nucleic acids research

authors： Xu Q,Yang C,Du Y,Chen Y,Liu H,Deng M,Zhang H,Zhang L,Liu T,Liu Q,Wang L,Lou Z,Pei H

更新日期：2014-05-01 00:00:00

abstract：:We describe a new, broadly applicable methodology for screening in parallel interactions of RNA-binding proteins (RBPs) with large numbers of microRNA (miRNA) precursors and for determining their affinities in native form in the presence of cellular factors. The assays aim at identifying pre-miRNAs that are potentiall...

journal_title：Nucleic acids research

authors： Towbin H,Wenter P,Guennewig B,Imig J,Zagalak JA,Gerber AP,Hall J

更新日期：2013-02-01 00:00:00

abstract：:p73, the p53 homologue, exists as a transactivation-domain-proficient TAp73 or deficient deltaN(DN)p73 form. Expectedly, the oncogenic DNp73 that is capable of inactivating both TAp73 and p53 function, is over-expressed in cancers. However, the role of TAp73, which exhibits tumour-suppressive properties in gain or los...

journal_title：Nucleic acids research

authors： Toh WH,Logette E,Corcos L,Sabapathy K

更新日期：2008-08-01 00:00:00

abstract：:Light is a major external factor in regulating seed germination. Photoreceptor phytochrome B (PHYB) plays a predominant role in promoting seed germination in the initial phase after imbibition, partially by repressing phytochrome-interacting factor1 (PIF1). However, the mechanism underlying the PHYB-PIF1-mediated tran...

journal_title：Nucleic acids research

authors： Gu D,Chen CY,Zhao M,Zhao L,Duan X,Duan J,Wu K,Liu X

更新日期：2017-07-07 00:00:00

abstract：:A vast number of sweet tasting molecules are known, encompassing small compounds, carbohydrates, d-amino acids and large proteins. Carbohydrates play a particularly big role in human diet. The replacement of sugars in food with artificial sweeteners is common and is a general approach to prevent cavities, obesity and ...

journal_title：Nucleic acids research

authors： Ahmed J,Preissner S,Dunkel M,Worth CL,Eckert A,Preissner R

更新日期：2011-01-01 00:00:00

abstract：:The small subunit (SSU) processome is a large ribonucleoprotein that is required for maturation of the 18S rRNA of the ribosome. Recently, a missense mutation in the C-terminus of an SSU processome protein, Utp4/Cirhin, was reported to cause North American Indian childhood cirrhosis (NAIC). In this study, we use Sacch...

journal_title：Nucleic acids research

authors： Freed EF,Baserga SJ

更新日期：2010-08-01 00:00:00

abstract：:In vitro repair of single strand breaks in T4 and phage DNA caused by 32p decay was studied. Zone centrifugation procedure showed that polynucleotide kinase, ligase enzyme system failed to repair 32P-damages. It was found that damaged DNA contained gaps and could be repaired by DNA-polymerase I, polynucleotide ligase ...

journal_title：Nucleic acids research

authors： Fodor I,Matvienko NI,Zlotnikov KM,Tanyashin VI,Solonin AS,Bayev AA

更新日期：1975-05-01 00:00:00