The nuclear 5S RNAs from chicken, rat and man. U5 RNAs are encoded by multiple genes.

abstract：:Cancer-associated somatic mutations outside protein-coding regions remain largely unexplored. Analyses of the TERT locus have indicated that non-coding regulatory mutations can be more frequent than previously suspected and play important roles in oncogenesis. Using a computational method called SASE-hunter, developed...

journal_title：Nucleic acids research

authors： Smith KS,Yadav VK,Pedersen BS,Shaknovich R,Geraci MW,Pollard KS,De S

更新日期：2015-06-23 00:00:00

abstract：:In this article, we introduce the Gypsy Database (GyDB) of mobile genetic elements, an in-progress database devoted to the non-redundant analysis and evolutionary-based classification of mobile genetic elements. In this first version, we contemplate eukaryotic Ty3/Gypsy and Retroviridae long terminal repeats (LTR) ret...

journal_title：Nucleic acids research

authors： Lloréns C,Futami R,Bezemer D,Moya A

更新日期：2008-01-01 00:00:00

abstract：:NF-IL6beta regulates gene expression and plays function roles in many tissues. The EGF-regulated cyclooxygenase-2 (cox-2) expression is mediated through p38(MAPK) signaling pathway and positively correlates with NF-IL6beta expression in A431 cells. NF-IL6beta coordinated with c-Jun on cox-2 transcriptional activation ...

journal_title：Nucleic acids research

authors： Wang JM,Ko CY,Chen LC,Wang WL,Chang WC

更新日期：2006-01-05 00:00:00

abstract：:The high-throughput sequencing of nuclease-protected mRNA fragments bound to ribosomes, a technique known as ribosome profiling, quantifies the relative frequencies with which different regions of transcripts are translated. This technique has revealed novel translation initiation sites with unprecedented scope and ha...

journal_title：Nucleic acids research

authors： Martens AT,Taylor J,Hilser VJ

更新日期：2015-04-20 00:00:00

abstract：:The role of the cyclic-AMP (cAMP) responsive element (CRE) in eukaryotic gene transcription was investigated in several cell lines transfected by constructs containing the chloramphenicol acetyltransferase (CAT) gene linked to the three different promoters, simian virus (SV) 40, human c-Ha-ras-1, or chicken beta-actin...

journal_title：Nucleic acids research

authors： Kanei-Ishii C,Ishii S

更新日期：1989-02-25 00:00:00

abstract：:YB-1 is a universal major protein of cytoplasmic mRNPs, a member of the family of multifunctional cold shock domain proteins (CSD proteins). Depending on its amount on mRNA, YB-1 stimulates or inhibits mRNA translation. In this study, we have analyzed complexes formed in vitro at various YB-1 to mRNA ratios, including...

journal_title：Nucleic acids research

authors： Skabkin MA,Kiselyova OI,Chernov KG,Sorokin AV,Dubrovin EV,Yaminsky IV,Vasiliev VD,Ovchinnikov LP

更新日期：2004-10-19 00:00:00

abstract：:Detailed models are presented for the triple-stranded polynucleotide helices of poly (U)-poly (A)-poly (U) (two forms), poly (U)-poly d (A) -poly (U), poly d(C)-poly d(I)-poly d(C), poly d(T)-polyd(A)-poly d(T) and poly (I)-poly (A)-poly (I). The models were genrated using a computerized, linked-atom procedure which p...

journal_title：Nucleic acids research

authors： Arnott S,Bond PJ,Selsing E,Smith PJ

更新日期：1976-10-01 00:00:00

abstract：:Protein species interacting with newly replicated DNA were analyzed using a photo cross-linking technique. Nascent DNA was labeled in vitro with [alpha-32P]dCTP and BrdUTP in SV40-infected CV-1 cells made permeable with saponin. The labeled cells were then irradiated with UV light (254 nm) and were treated extensively...

journal_title：Nucleic acids research

authors： Tsutsui K,Watanabe S,Katagiri M,Oda T

更新日期：1983-07-25 00:00:00

abstract：:Two DNA polymerases have been purified from the 105,000 x g supernatant of ungerminated wheat. The purification stages included: high speed centrifugation, salt fractionation, DEAE-cellulose chromatography, Sephadex G-150 filtration and phosphocellulose chromatography. Several properties of the two enzyme (called A an...

journal_title：Nucleic acids research

authors： Castroviejo M,Tarragó-Litvak L,Litvak S

更新日期：1975-11-01 00:00:00

abstract：:LINE-1 (L1) is a highly successful autonomous non-LTR retrotransposon and a major force shaping mammalian genomes. Although there are about 600 000 L1 copies covering 23% of the rat genome, full-length rat L1s (L1Rn) with intact open reading frames (ORFs) representing functional master copies for retrotransposition ha...

journal_title：Nucleic acids research

authors： Kirilyuk A,Tolstonog GV,Damert A,Held U,Hahn S,Löwer R,Buschmann C,Horn AV,Traub P,Schumann GG

更新日期：2008-02-01 00:00:00

abstract：:Novel techniques for high-throughput steady-state metabolomic profiling yield information about changes of nearly thousands of metabolites. Such metabolomic profiles, when analyzed together with transcriptional profiles, can reveal novel insights about underlying biological processes. While a number of conceptual appr...

journal_title：Nucleic acids research

authors： Sergushichev AA,Loboda AA,Jha AK,Vincent EE,Driggers EM,Jones RG,Pearce EJ,Artyomov MN

更新日期：2016-07-08 00:00:00

abstract：:Although subtelomeric regions in humans are heterochromatic, the epigenetic nature of human telomeres remains controversial. This controversy might have been influenced by the confounding effect of subtelomeric regions and interstitial telomeric sequences (ITSs) on telomeric chromatin structure analyses. In addition, ...

journal_title：Nucleic acids research

authors： Cubiles MD,Barroso S,Vaquero-Sedas MI,Enguix A,Aguilera A,Vega-Palas MA

更新日期：2018-03-16 00:00:00

abstract：:Ethidium causes the cleavage of DNA via a light and oxygen dependent process. Using covalently closed circular DNA as a substrate, the saturation kinetics and the dependence on superhelical density of the cleavage indicate that intercalated ethidium is mainly responsible for nicking DNA. Superoxide dismutase has littl...

journal_title：Nucleic acids research

authors： Deniss IS,Morgan AR

更新日期：1976-02-01 00:00:00

abstract：:The SPARC gene 5'flanking sequence has been shown to contain enhancer elements, but also negative control elements immediately upstream of the enhancer elements. Although these 5'enhancer elements are active in F9 and PYS-2 cells, their activities are nullified by the 5'repressor activity. In the present study we have...

journal_title：Nucleic acids research

authors： Satyamoorthy K,Samulewicz SJ,Thornburg LD,Basu A,Howe CC

更新日期：1997-08-01 00:00:00

abstract：:Studies involving ribozyme-directed inactivation of targeted RNA molecules have met with mixed success, making clear the importance of methods to measure and optimize ribozyme activity within cells. The interpretation of biochemical assays for determining ribozyme activity in the cellular environment have been complic...

journal_title：Nucleic acids research

authors： Seyhan AA,Amaral J,Burke JM

更新日期：1998-08-01 00:00:00

abstract：:Unfolding of the nucleosomes in transcriptionally active chromatin uncovers the sulfhydryl groups of histone H3 and permits the selective recovery of the unfolded nucleosomes by mercury-affinity chromatography. This new technique has been used to compare the nucleosomal proteins and their postsynthetic modifications i...

journal_title：Nucleic acids research

authors： Sterner R,Boffa LC,Chen TA,Allfrey VG

更新日期：1987-06-11 00:00:00

abstract：:The viral RNA (vRNA) genome of influenza viruses is replicated by the RNA-dependent RNA polymerase (RNAP) via a complementary RNA (cRNA) intermediate. The vRNA promoter can adopt multiple conformations when bound by the RNAP. However, the dynamics, determinants, and biological role of these conformations are unknown; ...

journal_title：Nucleic acids research

authors： Robb NC,Te Velthuis AJW,Fodor E,Kapanidis AN

更新日期：2019-07-09 00:00:00

abstract：:The recognition of human tRNA(Leu) or tRNA(Ser) by cognate aminoacyl- tRNA synthetases has distinct requirements. Only one base change (A73-->G) in tRNA(Leu) is required to generate an efficient serine acceptor in vitro, whereas several changes in three structural domains (the acceptor stem, DHU loop and long extra ar...

journal_title：Nucleic acids research

authors： Breitschopf K,Gross HJ

更新日期：1996-02-01 00:00:00

abstract：:Ribonucleotides are misincorporated into replicating DNA due to the similarity of deoxyribonucleotides and ribonucleotides, the high concentration of ribonucleotides in the nucleus and the imperfect accuracy of replicative DNA polymerases in choosing the base with the correct sugar. Embedded ribonucleotides change cer...

journal_title：Nucleic acids research

authors： Potenski CJ,Klein HL

更新日期：2014-01-01 00:00:00

abstract：:The method employed to determine the sequence of a T1 RNase fragment, A-A-A-A-A-U-A-A-C-A-A-U-A-C-A-Gp, from Novikoff rat hepatoma 18S ribosomal RNA is described. This method is applicable to any oligoribonucleotide produced by specific endonucleases that leave the newly cleaved 5'-end free for labeling with polynucle...

journal_title：Nucleic acids research

authors： Fuke M,Busch H

更新日期：1977-02-01 00:00:00

abstract：:Dimerization of HIV-1 genomic RNA is an essential step of the viral cycle, initiated at a conserved stem-loop structure which forms a 'kissing complex' involving loop-loop interactions (dimerization initiation site, DIS). A 19mer RNA oligonucleotide (DIS-19) has been synthesized which forms a stable symmetrical dimer ...

journal_title：Nucleic acids research

authors： Dardel F,Marquet R,Ehresmann C,Ehresmann B,Blanquet S

更新日期：1998-08-01 00:00:00

abstract：:Generally, the presence of a consensus sequence in the promoter of a gene is taken as indication for regulation by the transcription factor that binds to this sequence. In light of the recent developments in genome research, we were interested to what extent this supposition is valid. We examined the relationship betw...

journal_title：Nucleic acids research

authors： Hamoen LW,Smits WK,de Jong A,Holsappel S,Kuipers OP

更新日期：2002-12-15 00:00:00

abstract：:Microarrays are an effective tool for monitoring genome-wide gene expression levels. In current microarray analyses, the majority of genes on arrays are frequently eliminated for further analysis because the changes in their expression levels (ratios) are considered to be not significant. This strategy risks failure t...

journal_title：Nucleic acids research

authors： Yano K,Imai K,Shimizu A,Hanashita T

更新日期：2006-03-14 00:00:00

abstract：:Dramatic improvements in ribozyme mimics have been achieved by employing the principles of small molecule catalysis to the design of macromolecular, biomimetic reagents. Ribozyme mimics derived from the ligand 2,9-dimethylphenanthroline (neocuproine) show at least 30-fold improvements in efficiency at sequence-specifi...

journal_title：Nucleic acids research

authors： Putnam WC,Daniher AT,Trawick BN,Bashkin JK

更新日期：2001-05-15 00:00:00

abstract：:GeneAlign is a coding exon prediction tool for predicting protein coding genes by measuring the homologies between a sequence of a genome and related sequences, which have been annotated, of other genomes. Identifying protein coding genes is one of most important tasks in newly sequenced genomes. With increasing numbe...

journal_title：Nucleic acids research

authors： Hsieh SJ,Lin CY,Liu NH,Chow WY,Tang CY

更新日期：2006-07-01 00:00:00

abstract：:We analysed the basic mechanisms of signal transmission in DNA and the origins of the allostery exhibited by systems such as the ternary complex BAMHI-DNA-GRDBD. We found that perturbation information generated by a primary protein binding event travels as a wave to distant regions of DNA following a hopping mechanism...

journal_title：Nucleic acids research

authors： Balaceanu A,Pérez A,Dans PD,Orozco M

更新日期：2018-09-06 00:00:00

abstract：:The human Werner syndrome protein, WRN, is a member of the RecQ helicase family and contains 3'-->5' helicase and 3'-->5' exonuclease activities. Recently, we showed that the exonuclease activity of WRN is greatly stimulated by the human Ku heterodimer protein. We have now mapped this interaction physically and functi...

journal_title：Nucleic acids research

authors： Karmakar P,Snowden CM,Ramsden DA,Bohr VA

更新日期：2002-08-15 00:00:00

abstract：:We have reported on the differences in site-specific cleavage between DNA and DNA-RNA hybrids by various prototypic DNA cleavers (accompanying paper). In the case of bleomycin (BLM), degradation at 5'-GC-3'sites was suppressed relative to the same sequence in double-stranded DNA, while 5'-GT-3' damage remained constan...

journal_title：Nucleic acids research

authors： Bansal M,Stubbe J,Kozarich JW

更新日期：1997-05-01 00:00:00

abstract：:During telomere replication in yeast, chromosome ends acquire a long single-stranded extension of the strand making the 3' end. Previous work showed that these 3' tails are generated late in S-phase, when conventional replication is virtually complete. In addition, the extensions were also observed in cells that lacke...

journal_title：Nucleic acids research

authors： Dionne I,Wellinger RJ

更新日期：1998-12-01 00:00:00

abstract：:The nuclear receptor vitamin D receptor (VDR) is known to associate with two vitamin D response element (VDRE) containing chromatin regions of the insulin-like growth factor binding protein 3 (IGFBP3) gene. In non-malignant MCF-10A human mammary cells, we show that the natural VDR ligand 1α,25-dihydroxyvitamin D(3) (1...

journal_title：Nucleic acids research

authors： Malinen M,Ryynänen J,Heinäniemi M,Väisänen S,Carlberg C

更新日期：2011-01-01 00:00:00