Abstract:
:Two DNA polymerases have been purified from the 105,000 x g supernatant of ungerminated wheat. The purification stages included: high speed centrifugation, salt fractionation, DEAE-cellulose chromatography, Sephadex G-150 filtration and phosphocellulose chromatography. Several properties of the two enzyme (called A and B according to the order of elution from the phosphocellulose column) have been studied. Enzyme A has a sedimentation coefficient of about 7 S, utilizes activated DNA and synthetic polydeoxynucleotides as well as poly rA-dT12, while B has a sedimentation coefficient of about 6.2 and uses only activated DNA and synthetic polydeoxynucleotides as templates. Other parameters like KCl effect, MnCl2 effect, optimum pH, etc. Allow us to distinguish clearly between both DNA polymerases.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Castroviejo M,Tarragó-Litvak L,Litvak Sdoi
10.1093/nar/2.11.2077keywords:
subject
Has Abstractpub_date
1975-11-01 00:00:00pages
2077-90issue
11eissn
0305-1048issn
1362-4962journal_volume
2pub_type
杂志文章abstract::The exact sites at which a number of drugs inhibit the nick translation of DNA by E.coli DNA polymerase-I have been pinpointed. In order to do this, a method has been developed for sequencing double-stranded plasmid DNA from the site of a specifically induced nick. The initial experiments have concentrated on analysis...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/10.22.7273
更新日期:1982-11-25 00:00:00
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journal_title:Nucleic acids research
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doi:10.1093/nar/12.12.4921
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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更新日期:2017-01-04 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkf508
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx859
更新日期:2017-12-15 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2008-08-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2013-02-01 00:00:00
abstract::The molecular organization of the dunce gene of Drosophila melanogaster has proved to be particularly complex, with two divergently transcribed genes, Sgs-4 and Pig-1, nested within its 79 kb intron (1). Here we report the identification and the molecular characterization of a third gene nested within the transcriptio...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/18.19.5837
更新日期:1990-10-11 00:00:00
abstract::Initiation of bacterial DNA replication at oriC is mediated by primosomal proteins that act cooperatively to melt an AT-rich region where the replicative helicase is loaded prior to the assembly of the replication fork. In Bacillus subtilis, the dnaD, dnaB and dnaI genes are essential for initiation of DNA replication...
journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2010-05-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2005-12-16 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/26.7.1761
更新日期:1998-04-01 00:00:00
abstract::The mutation patterns at Cas9 targeted sites contain unique information regarding the nuclease activity and repair mechanisms in mammalian cells. However, analytical framework for extracting such information are lacking. Here, we present a novel computational platform called Rational InDel Meta-Analysis (RIMA) that en...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky653
更新日期:2018-09-19 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1983-05-11 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkh280
更新日期:2004-02-20 00:00:00
abstract::ATF4 is a pro-oncogenic transcription factor whose translation is activated by eIF2 phosphorylation through delayed re-initiation involving two uORFs in the mRNA leader. However, in yeast, the effect of eIF2 phosphorylation can be mimicked by eIF5 overexpression, which turns eIF5 into translational inhibitor, thereby ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw559
更新日期:2016-10-14 00:00:00
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journal_title:Nucleic acids research
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更新日期:2003-12-15 00:00:00
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journal_title:Nucleic acids research
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更新日期:2015-07-27 00:00:00
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp1230
更新日期:2010-05-01 00:00:00
abstract::The Homeodomain Resource is a comprehensive collection of sequence, structure and genomic information on the homeodomain protein family. Available through the Resource are both full-length and domain-only sequence data, as well as X-ray and NMR structural data for proteins and protein-DNA complexes. Also available is ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/27.1.336
更新日期:1999-01-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/11.19.6611
更新日期:1983-10-11 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt512
更新日期:2013-08-01 00:00:00
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journal_title:Nucleic acids research
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更新日期:1988-02-11 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq823
更新日期:2011-02-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr436
更新日期:2011-08-01 00:00:00