Abstract：
:LINE1s occupy 17% of the human genome and are its only active autonomous mobile DNA. L1s are also responsible for genomic insertion of processed pseudogenes and >1 million non-autonomous retrotransposons (Alus and SVAs). These elements have significant effects on gene organization and expression. Despite the importance of retrotransposons for genome evolution, much about their biology remains unknown, including cellular factors involved in the complex processes of retrotransposition and forming and transporting L1 ribonucleoprotein particles. By co-immunoprecipitation of tagged L1 constructs and mass spectrometry, we identified proteins associated with the L1 ORF1 protein and its ribonucleoprotein. These include RNA transport proteins, gene expression regulators, post-translational modifiers, helicases and splicing factors. Many cellular proteins co-localize with L1 ORF1 protein in cytoplasmic granules. We also assayed the effects of these proteins on cell culture retrotransposition and found strong inhibiting proteins, including some that control HIV and other retroviruses. These data suggest candidate cofactors that interact with the L1 to modulate its activity and increase our understanding of the means by which the cell coexists with these genomic 'parasites'.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Goodier JL,Cheung LE,Kazazian HH Jrdoi
10.1093/nar/gkt512subject
Has Abstractpub_date
2013-08-01 00:00:00pages
7401-19issue
15eissn
0305-1048issn
1362-4962pii
gkt512journal_volume
41pub_type
杂志文章abstract：:We have analysed DNA modification in a HapII site (CCGG) present in the major intron of the discontinuous rabbit beta-globin gene. In most somatic tissues, including erythroid and non-erythroid tissues, about 50% of the DNA is resistant to cleavage at this site by HapII, though 100% cleavage is found with the isoschiz...
journal_title：Nucleic acids research
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journal_title：Nucleic acids research
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更新日期：2012-01-01 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm690
更新日期：2008-01-01 00:00:00
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pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：2017-07-27 00:00:00
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更新日期：2010-03-01 00:00:00
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更新日期：2005-07-13 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz906
更新日期：2019-12-02 00:00:00
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更新日期：2010-01-01 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章,评审
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更新日期：2008-08-01 00:00:00
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pub_type： 杂志文章
doi：10.1093/nar/13.5.1637
更新日期：1985-03-11 00:00:00
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pub_type： 杂志文章
doi：10.1093/nar/26.11.2723
更新日期：1998-06-01 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/8.7.1535
更新日期：1980-04-11 00:00:00
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journal_title：Nucleic acids research
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更新日期：2006-01-01 00:00:00
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更新日期：2019-09-19 00:00:00
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journal_title：Nucleic acids research
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更新日期：2017-04-20 00:00:00
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更新日期：1991-12-25 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
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更新日期：1974-08-01 00:00:00
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更新日期：2021-01-08 00:00:00
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更新日期：2010-06-01 00:00:00
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更新日期：2012-01-01 00:00:00
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journal_title：Nucleic acids research
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更新日期：2015-05-19 00:00:00
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更新日期：2011-04-01 00:00:00
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更新日期：2008-01-01 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
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更新日期：1993-11-11 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
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更新日期：1992-04-11 00:00:00
