Activation of neocarzinostatin chromophore and formation of nascent DNA damage do not require molecular oxygen.

abstract：:We have amplified the large subunit ribosomal DNA (LSUrDNA) of the 12 described Naegleria spp. and of 34 other Naegleria lineages that might be distinct species. Two strains yielded a product that is longer than 3 kb, which is the length of the LSUrDNA of all described Naegleria spp. Sequencing data revealed that the ...

journal_title：Nucleic acids research

authors： De Jonckheere JF,Brown S

更新日期：1998-01-15 00:00:00

abstract：:Spliced leader (SL) trans-splicing is a critical element of gene expression in a number of eukaryotic groups. This process is arguably best understood in nematodes, where biochemical and molecular studies in Caenorhabditis elegans and Ascaris suum have identified key steps and factors involved. Despite this, the preci...

journal_title：Nucleic acids research

authors： Philippe L,Pandarakalam GC,Fasimoye R,Harrison N,Connolly B,Pettitt J,Müller B

更新日期：2017-08-21 00:00:00

abstract：:A comparison of gene structure, sequence, and transcription pattern of heat shock loci 93D of Drosophila melanogaster and 48B of Drosophila hydei has been performed. Both heat shock loci consist of an unique region that is flanked by an internally repetitive element. Different members of these elements are highly cons...

journal_title：Nucleic acids research

authors： Ryseck RP,Walldorf U,Hoffmann T,Hovemann B

更新日期：1987-04-24 00:00:00

abstract：:The ribosomal frameshifting signal of the mouse embryonal carcinoma differentiation regulated (Edr) gene represents the sole documented example of programmed -1 frameshifting in mammalian cellular genes [Shigemoto,K., Brennan,J., Walls,E,. Watson,C.J., Stott,D., Rigby,P.W. and Reith,A.D. (2001), Nucleic Acids Res., 29...

journal_title：Nucleic acids research

authors： Manktelow E,Shigemoto K,Brierley I

更新日期：2005-03-14 00:00:00

abstract：:A putative role for mammalian polynucleotide kinases that possess both 5'-phosphotransferase and 3'-phosphatase activity is the restoration of DNA strand breaks with 5'-hydroxyl termini or 3'-phosphate termini, or both, to a form that supports the subsequent action of DNA repair polymerases and DNA ligases, i.e. 5'-ph...

journal_title：Nucleic acids research

authors： Karimi-Busheri F,Lee J,Tomkinson AE,Weinfeld M

更新日期：1998-10-01 00:00:00

abstract：:As the largest group of MYB family transcription factors, R2R3-MYB proteins play essential roles during plant growth and development. However, the structural basis underlying how R2R3-MYBs recognize the target DNA remains elusive. Here, we report the crystal structure of Arabidopsis WEREWOLF (WER), an R2R3-MYB protein...

journal_title：Nucleic acids research

authors： Wang B,Luo Q,Li Y,Yin L,Zhou N,Li X,Gan J,Dong A

更新日期：2020-01-10 00:00:00

abstract：:Extracts of the human glioma cell line A1235 (lacking O(6)-methylguanine-DNA methyltransferase) are known to restore a G:T mismatch to a normal G:C pair in a G:T-containing model (45 bp) DNA substrate. Herein we demonstrate that substitution of G:T with O(6)-methylguanine:T (m6G:T) results in extract-induced intra-str...

journal_title：Nucleic acids research

authors： Lari SU,Day RS,Dobler K,Paterson MC

更新日期：2001-06-01 00:00:00

abstract：:ORegAnno is an open-source, open-access database and literature curation system for community-based annotation of experimentally identified DNA regulatory regions, transcription factor binding sites and regulatory variants. The current release comprises 30 145 records curated from 922 publications and describing regul...

journal_title：Nucleic acids research

authors： Griffith OL,Montgomery SB,Bernier B,Chu B,Kasaian K,Aerts S,Mahony S,Sleumer MC,Bilenky M,Haeussler M,Griffith M,Gallo SM,Giardine B,Hooghe B,Van Loo P,Blanco E,Ticoll A,Lithwick S,Portales-Casamar E,Donaldson IJ,

更新日期：2008-01-01 00:00:00

abstract：:Evidence will be presented to show that the allyl group is suitable for the protection of a 3'-terminal phosphodiester function. The latter will be demonstrated by the synthesis, via a phosphotriester approach, of two cyclic tetraribonucleotides [r(AAAA) and r(UAMe2UAMe2)], two cyclic hexadeoxyribonucleotides [d(CGCGC...

journal_title：Nucleic acids research

authors： de Vroom E,Broxterman HJ,Sliedregt LA,van der Marel GA,van Boom JH

更新日期：1988-05-25 00:00:00

abstract：:A terminator of transcription with bidirectional activity has been located between the translation termination codons of the genes tetA and orfL on Tn10. These genes are transcribed towards each other. Each orientation of the intervening sequence is shown to reduce the expression of the lacZ and galK genes when cloned...

journal_title：Nucleic acids research

authors： Schollmeier K,Gärtner D,Hillen W

更新日期：1985-06-25 00:00:00

abstract：:Redefinition of UAG, UAA and UGA to specify a standard amino acid occurs in response to recoding signals present in a minority of mRNAs. This 'read-through' is in competition with termination and is utilized for gene expression. One of the recoding signals known to stimulate read-through is a hexanucleotide sequence o...

journal_title：Nucleic acids research

authors： Harrell L,Melcher U,Atkins JF

更新日期：2002-05-01 00:00:00

abstract：:To explain the activation of transcription by a remote enhancer, two models are most often considered, namely looping and scanning. A scanning model, also referred to as 'polymerase entry site' model predicts that for two adjacent promoters the one proximal to an enhancer would be preferentially activated. Preferentia...

journal_title：Nucleic acids research

authors： Heuchel R,Matthias P,Schaffner W

更新日期：1989-11-25 00:00:00

abstract：:Large differences in plant genome sizes are mainly due to numerous events of insertions or deletions (indels). The balance between these events determines the evolutionary direction of genome changes. To address the question of what phenomena trigger these alterations, we compared the genomic sequences of two Arabidop...

journal_title：Nucleic acids research

authors： Ziolkowski PA,Koczyk G,Galganski L,Sadowski J

更新日期：2009-06-01 00:00:00

abstract：:The discoidin proteins of Dictyostelium discoideum are highly expressed during development. The Disc I gamma promoter allows the regulation of heterologous protein expression by experimental conditions. We report conditions under which the promoter activity is efficiently repressed during growth in the wildtype strain...

journal_title：Nucleic acids research

authors： Wetterauer BW,Salger K,MacWilliams HK

更新日期：1993-03-25 00:00:00

abstract：:The alpha/beta-hydrolase fold is characterized by a beta-sheet core of five to eight strands connected by alpha-helices to form a alpha/beta/alpha sandwich. In most of the family members the beta-strands are parallels, but some show an inversion in the order of the first strands, resulting in antiparallel orientation....

journal_title：Nucleic acids research

authors： Hotelier T,Renault L,Cousin X,Negre V,Marchot P,Chatonnet A

更新日期：2004-01-01 00:00:00

abstract：:In vivo and in vitro RNA splicing experiments have demonstrated that the intron splicing machineries are not interchangeable in all organisms. These differences have prevented the efficient in vivo expression of monocot genes containing introns in dicot plants and the in vitro excision of some plant introns in HeLa ce...

journal_title：Nucleic acids research

authors： Hanley BA,Schuler MA

更新日期：1988-07-25 00:00:00

abstract：:The abundance of Alu RNA is transiently increased by heat shock in human cell lines. This effect is specific to Alu repeats among Pol III transcribed genes, since the abundance of 7SL, 7SK, 5S and U6 RNAs is essentially unaffected by heat shock. The rapid induction of Alu expression precedes the heat shock induction o...

journal_title：Nucleic acids research

authors： Liu WM,Chu WM,Choudary PV,Schmid CW

更新日期：1995-05-25 00:00:00

abstract：:Pseudomonas aeruginosa is an opportunistic pathogen with a relatively large genome, and has been shown to routinely lose genomic fragments during environmental selection. However, the underlying molecular mechanisms that promote chromosomal deletion are still poorly understood. In a recent study, we showed that by del...

journal_title：Nucleic acids research

authors： Shen M,Zhang H,Shen W,Zou Z,Lu S,Li G,He X,Agnello M,Shi W,Hu F,Le S

更新日期：2018-05-18 00:00:00

abstract：:We have examined the ability of a cationic planar chromophore, ethidium bromide, to intercalate into a short, defined triple helix. Using UV absorption, fluorescence spectroscopy and a gel retardation assay we demonstrate that ethidium bromide is able to bind to a triple helix with a lower affinity than to the corresp...

journal_title：Nucleic acids research

authors： Mergny JL,Collier D,Rougée M,Montenay-Garestier T,Hélène C

更新日期：1991-04-11 00:00:00

abstract：:The 'sandwich' binding format, which uses two reagents that can bind simultaneously to a given analyte, is the gold standard in diagnostics and many biochemical techniques. One of the bottlenecks in creating a sandwich assay is identifying pairs of reagents that bind non-competitively to the target. To bridge this gap...

journal_title：Nucleic acids research

authors： Gorman KT,Roby LC,Giuffre A,Huang R,Kay BK

更新日期：2017-10-13 00:00:00

abstract：:Stability of aromatic phosphoramidates was studied using 2',3'-O-dibenzoyluridine 5'-phosphoramidates and N,2',3'-O-tribenzoylcytidine 5'-phosphate. The effect of dicyclohexylcarbodiimide in this mixture was investigated. Decomposition of the anilidate was slower in the presence of DCC. Substituted anilidates of uridi...

journal_title：Nucleic acids research

authors： Otsuka E,Honda A,Shigyo H,Morioka S,Sugiyama T

更新日期：1974-02-01 00:00:00

abstract：:The circular dichroism spectra of Hg(II) complexes with native calf thymus DNA, chemically methylated Streptomyces chrysomallus DNA and with Ag(I)-DNA complexes were measured in the region of 220 - 340 nm. As a main result a conversion of the conservative CD spectrum of DNA to a distinct nonconservative type of CD spe...

journal_title：Nucleic acids research

authors： Walter A,Luck G

更新日期：1977-03-01 00:00:00

abstract：:We have used DNase I footprinting to examine the association kinetics of GA-, GT- and CT-containing oligonucleotides with the target sequence (GGA)5GG. (CCT)5CC. These reactions are slow yielding bimolecular association rate constants between 50 and 2000 M-1s-1. We find that GT-containing oligonucleotides bind much fa...

journal_title：Nucleic acids research

authors： Paes HM,Fox KR

更新日期：1997-08-15 00:00:00

abstract：:Well defined biomacromolecular patterns such as binding sites, catalytic sites, specific protein or nucleic acid sequences, etc. precisely modulate many important biological phenomena. We introduce PatternQuery, a web-based application designed for detection and fast extraction of such patterns. The application uses a...

journal_title：Nucleic acids research

authors： Sehnal D,Pravda L,Svobodová Vařeková R,Ionescu CM,Koča J

更新日期：2015-07-01 00:00:00

abstract：:Targeted gene silencing by RNA interference allows the study of gene function in plants and animals. In cell culture and small animal models, genetic screens can be performed--even tissue-specifically in Drosophila--with genome-wide RNAi libraries. However, a major problem with the use of RNAi approaches is the unavoi...

journal_title：Nucleic acids research

authors： Schulz JG,David G,Hassan BA

更新日期：2009-07-01 00:00:00

abstract：:Mycobacterium tuberculosis (Mtb) Cmr (Rv1675c) is a CRP/FNR family transcription factor known to be responsive to cAMP levels and during macrophage infections. However, Cmr's DNA binding properties, cellular targets and overall role in tuberculosis (TB) complex bacteria have not been characterized. In this study, we u...

journal_title：Nucleic acids research

authors： Ranganathan S,Bai G,Lyubetskaya A,Knapp GS,Peterson MW,Gazdik M,C Gomes AL,Galagan JE,McDonough KA

更新日期：2016-01-08 00:00:00

abstract：:The aspartate protease of Mucor pusillus (Mucor pusillus rennin; MPR) is a milk-clotting enzyme used in the cheese industry. The partial amino acid sequence of MPR was determined and oligonucleotide probes were synthesized for cloning of the MPR gene. A clone giving positive hybridization with the probes was selected ...

journal_title：Nucleic acids research

authors： Tonouchi N,Shoun H,Uozumi T,Beppu T

更新日期：1986-10-10 00:00:00

abstract：:Adjustable transgene expression is considered key for next-generation molecular interventions in gene therapy scenarios, therapeutic reprogramming of clinical cell phenotypes for tissue engineering and sophisticated gene-function analyses in the post-genomic era. We have designed a portfolio of latest generation self-...

journal_title：Nucleic acids research

authors： Mitta B,Weber CC,Rimann M,Fussenegger M

更新日期：2004-07-16 00:00:00

abstract：:Our database on large ribosomal subunit RNA contained 334 sequences in July, 1995. All sequences in the database are aligned, taking into account secondary structure. The aligned sequences are provided, together with incorporated secondary structure information, in several computer-readable formats. These data can eas...

journal_title：Nucleic acids research

authors： De Rijk P,Van de Peer Y,De Wachter R

更新日期：1996-01-01 00:00:00

abstract：:A linear method for the search of eukaryotic nuclear tRNA genes in DNA databases is described. Based on a modified version of the general weight matrix procedure, our algorithm relies on the recognition of two intragenic control regions known as A and B boxes, a transcription termination signal, and on the evaluation ...

journal_title：Nucleic acids research

authors： Pavesi A,Conterio F,Bolchi A,Dieci G,Ottonello S

更新日期：1994-04-11 00:00:00