A bidirectionally active signal for termination of transcription is located between tetA and orfL on transposon Tn10.

abstract：:Clustered regularly interspaced short palindromic repeat (CRISPR) RNA-guided nucleases have gathered considerable excitement as a tool for genome engineering. However, questions remain about the specificity of target site recognition. Cleavage specificity is typically evaluated by low throughput assays (T7 endonucleas...

journal_title：Nucleic acids research

authors： O'Geen H,Henry IM,Bhakta MS,Meckler JF,Segal DJ

更新日期：2015-03-31 00:00:00

abstract：:Two new endonuclease activities, endonuclease B and endonuclease C, obtained from yeast nuclear preparations have been separated and partially characterized. Endonuclease B has a primary requirement for Mn2+ which cannot be replaced by Mg2+ or Ca2+, and makes single-strand scissions in double-stranded DNA. Endonculeas...

journal_title：Nucleic acids research

authors： Pinon R,Leney E

更新日期：1975-07-01 00:00:00

abstract：:We show that nucleic acid structures may be conveniently and inexpensively characterized by their UV thermal difference spectra. A thermal difference spectrum (TDS) is obtained for a nucleic acid by simply recording the ultraviolet absorbance spectra of the unfolded and folded states at temperatures above and below it...

journal_title：Nucleic acids research

authors： Mergny JL,Li J,Lacroix L,Amrane S,Chaires JB

更新日期：2005-09-12 00:00:00

abstract：:In the filamentous cyanobacterium Anabaena sp. PCC 7120, the ferric uptake regulator FurA functions as a global transcriptional regulator. Despite several analyses have focused on elucidating the FurA-regulatory network, the number of target genes described for this essential transcription factor is limited to a handf...

journal_title：Nucleic acids research

authors： González A,Angarica VE,Sancho J,Fillat MF

更新日期：2014-04-01 00:00:00

abstract：:A mass culture of human diploid fibroblasts, and eight clones isolated from that mass culture, were examined for methylation patterns in several regions of DNA. Plasmid-inserted cDNA sequences were used as probes for alpha-hCG, beta-globin, A gamma- and G gamma-globin, and beta- and gamma-actin gene regions. Each prob...

journal_title：Nucleic acids research

authors： Shmookler Reis RJ,Goldstein S

更新日期：1982-07-24 00:00:00

abstract：:Small RNA (sRNA) Sequencing technology has revealed that microRNAs (miRNAs) are capable of exhibiting frequent variations from their canonical sequences, generating multiple variants: the isoforms of miRNAs (isomiRs). However, integrated tool to precisely detect and systematically annotate isomiRs from sRNA sequencing...

journal_title：Nucleic acids research

authors： Zhang Y,Zang Q,Zhang H,Ban R,Yang Y,Iqbal F,Li A,Shi Q

更新日期：2016-07-08 00:00:00

abstract：:The high mobility group protein (HMG)-box is a DNA-binding domain found in many proteins that bind preferentially to DNA of irregular structures in a sequence-independent manner and can bend the DNA. We show here that GST-fusion proteins of HMG domains from HMG1 and HMG2 promote a triple-stranded complex formation bet...

journal_title：Nucleic acids research

authors： Suda T,Mishima Y,Takayanagi K,Asakura H,Odani S,Kominami R

更新日期：1996-12-01 00:00:00

abstract：:Aryldiazonium salts are shown to be useful as phototriggered structural probes for RNA mapping as well as for footprinting of RNA/protein interaction. In particular the yeast tRNA(Asp)/aspartyl-tRNA synthetase complex is shown to involve the variable loop face and the concave side of the L-shaped nucleic acid bound to...

journal_title：Nucleic acids research

authors： Garcia A,Giegé R,Behr JP

更新日期：1990-01-11 00:00:00

abstract：:A feature of Haemophilus influenzae genomes is the presence of several loci containing tracts of six or more identical tetranucleotide repeat units. These repeat tracts are unstable and mediate high frequency, reversible alterations in the expression of surface antigens. This process, termed phase variation (PV), enab...

journal_title：Nucleic acids research

authors： Bayliss CD,Sweetman WA,Moxon ER

更新日期：2005-01-14 00:00:00

abstract：:Eukaryotic topoisomerase II (topo II) is the essential decatenase of newly replicated chromosomes and the main relaxase of nucleosomal DNA. Apart from these general tasks, topo II participates in more specialized functions. In mammals, topo IIα interacts with specific RNA polymerases and chromatin-remodeling complexes...

journal_title：Nucleic acids research

authors： Nikolaou C,Bermúdez I,Manichanh C,García-Martinez J,Guigó R,Pérez-Ortín JE,Roca J

更新日期：2013-11-01 00:00:00

abstract：:In an extensive analysis, using a range of restriction endonucleases, HinfI and TaqI were found to differentiate satellites I, II and III & IV. Satellite I is resistant to digestion by TaqI, but is cleaved by HinfI to yield three major fragments of approximate size 770, 850 and 950bp, associated in a single length of ...

journal_title：Nucleic acids research

authors： Frommer M,Prosser J,Tkachuk D,Reisner AH,Vincent PC

更新日期：1982-01-22 00:00:00

abstract：:We have examined the absorption and circular dichroism spectra of intact Penicillium chrysogenum virus, empty capsid particles, and isolated double-stranded RNA. The absorbance at 260 nm of intact virus was less than 4% hypochromic relative to the absorbances of the free double-stranded RNA and free viral protein, ind...

journal_title：Nucleic acids research

authors： Edmondson SP,Gray DM

更新日期：1983-01-11 00:00:00

abstract：:The structures of the termini and their flanking regions of two human KpnI family members were investigated. The two differed in length, but the starting sequence at one terminal (defined as the 5' terminal) was found to be common to both members. The Alu family sequence was found in the 5' flanking regions. The KpnI ...

journal_title：Nucleic acids research

authors： Miyake T,Migita K,Sakaki Y

更新日期：1983-10-11 00:00:00

abstract：:FlyBase is a database of genetic and molecular data concerning Drosophila. FlyBase is maintained as a relational database (in Sybase). The scope of FlyBase includes: genes, alleles (and phenotypes), aberrations, pointers to sequence data, clones, stock lists, Drosophila workers and bibliographic references. FlyBase is...

journal_title：Nucleic acids research

authors： Flybase Consortium.

更新日期：1996-01-01 00:00:00

abstract：:The direct sequencing of DNA generated by the polynucleotide chain reaction, via the incorporation of phosphorothioate nucleotides and followed by treatment with an alkylating reagent that cleaves specifically at the phosphorothioate positions, is described. The Taq polymerase used in the amplification reaction incorp...

journal_title：Nucleic acids research

authors： Nakamaye KL,Gish G,Eckstein F,Vosberg HP

更新日期：1988-11-11 00:00:00

abstract：:Patients with Alzheimer's disease (AD) exhibit higher levels of 8-oxo-guanine (8-oxoG) DNA lesions in their brain, suggesting a reduced or defective 8-oxoG repair. To test this hypothesis, this study investigated 14 AD patients and 10 age-matched controls for mutations of the major 8-oxoG removal gene OGG1. Whereas no...

journal_title：Nucleic acids research

authors： Mao G,Pan X,Zhu BB,Zhang Y,Yuan F,Huang J,Lovell MA,Lee MP,Markesbery WR,Li GM,Gu L

更新日期：2007-01-01 00:00:00

abstract：:Natural antisense transcripts are at least partially complementary to their sense transcripts. Cis-Sense/Antisense pairs (cis-SAs) have been extensively characterized and known to play diverse regulatory roles, whereas trans-Sense/Antisense pairs (trans-SAs) in animals are poorly studied. We identified long trans-SAs ...

journal_title：Nucleic acids research

authors： Li JT,Zhang Y,Kong L,Liu QR,Wei L

更新日期：2008-09-01 00:00:00

abstract：:Oligonucleotide-directed mutagenesis was used to introduce an A to C transversion at position 523 in the 16S ribosomal RNA gene of Escherichia coli rrnB operon cloned in plasmid pKK3535. E. coli cells transformed with the mutated plasmid were resistant to streptomycin. The mutated ribosomes isolated from these cells w...

journal_title：Nucleic acids research

authors： Melançon P,Lemieux C,Brakier-Gingras L

更新日期：1988-10-25 00:00:00

abstract：:Within a single infected individual, a virus population can have a high genomic variability. In the case of HIV, several mutations can be present even in a small genomic window of 20-30 nucleotides. For diagnostics purposes, it is often needed to resequence genomic subsets where crucial mutations are known to occur. I...

journal_title：Nucleic acids research

authors： Hadiwikarta WW,Van Dorst B,Hollanders K,Stuyver L,Carlon E,Hooyberghs J

更新日期：2013-10-01 00:00:00

abstract：:Post-translational modifications (such as ubiquitination) of clock proteins are critical in maintaining the precision and robustness of the evolutionarily conserved circadian clock. Ubiquitination of the core clock transcription factor BMAL1 (brain and muscle Arnt-like 1) has recently been reported. However, it remain...

journal_title：Nucleic acids research

authors： Gossan NC,Zhang F,Guo B,Jin D,Yoshitane H,Yao A,Glossop N,Zhang YQ,Fukada Y,Meng QJ

更新日期：2014-05-01 00:00:00

abstract：:The RecQL4 helicase is involved in the maintenance of genome integrity and DNA replication. Mutations in the human RecQL4 gene cause the Rothmund-Thomson, RAPADILINO and Baller-Gerold syndromes. Mouse models and experiments in human and Xenopus have proven the N-terminal part of RecQL4 to be vital for cell growth. We ...

journal_title：Nucleic acids research

authors： Ohlenschläger O,Kuhnert A,Schneider A,Haumann S,Bellstedt P,Keller H,Saluz HP,Hortschansky P,Hänel F,Grosse F,Görlach M,Pospiech H

更新日期：2012-09-01 00:00:00

abstract：:The generalized mismatch repair system of Streptococcus pneumoniae (the Hex system) can eliminate base pair mismatches arising in heteroduplex DNA during transformation or by DNA polymerase errors during replication. Mismatch repair is most likely initiated at nicks or gaps. The present work was started to examine the...

journal_title：Nucleic acids research

authors： Méjean V,Devedjian JC,Rives I,Alloing G,Claverys JP

更新日期：1991-10-25 00:00:00

abstract：:TATA-binding protein (TBP) is central to the regulation of eukaryotic transcription initiation. Recruitment of TBP to target genes can be positively regulated by one of two basal transcription factor complexes: SAGA or TFIID. Negative regulation of TBP promoter association can be performed by Mot1p or the NC2 complex....

journal_title：Nucleic acids research

authors： Spedale G,Meddens CA,Koster MJ,Ko CW,van Hooff SR,Holstege FC,Timmers HT,Pijnappel WW

更新日期：2012-02-01 00:00:00

abstract：:ETS-domain transcription factors play important roles in controlling gene expression in a variety of different contexts; however, these proteins bind to very similar sites and it is unclear how in vivo specificity is achieved. In silico analysis is unlikely to reveal specific targets for individual family members and ...

journal_title：Nucleic acids research

authors： Boros J,O'Donnell A,Donaldson IJ,Kasza A,Zeef L,Sharrocks AD

更新日期：2009-12-01 00:00:00

abstract：:To achieve drug resistance Leishmania parasite alters gene copy number by using its repeated sequences widely distributed through the genome. Even though homologous recombination (HR) is ascribed to maintain genome stability, this eukaryote exploits this potent mechanism driven by the Rad51 recombinase to form benefic...

journal_title：Nucleic acids research

authors： Genois MM,Plourde M,Éthier C,Roy G,Poirier GG,Ouellette M,Masson JY

更新日期：2015-03-11 00:00:00

abstract：:The human Werner syndrome protein, WRN, is a member of the RecQ helicase family and contains 3'-->5' helicase and 3'-->5' exonuclease activities. Recently, we showed that the exonuclease activity of WRN is greatly stimulated by the human Ku heterodimer protein. We have now mapped this interaction physically and functi...

journal_title：Nucleic acids research

authors： Karmakar P,Snowden CM,Ramsden DA,Bohr VA

更新日期：2002-08-15 00:00:00

abstract：:Transcription of tRNA-encoding genes by RNA polymerase (Pol) III requires the six-subunit general transcription factor IIIC that uses subcomplexes τA and τB to recognize two gene-internal promoter elements named A- and B-box. The Schizosaccharomyces pombe τA subcomplex comprises subunits Sfc1, Sfc4 and Sfc7. The cryst...

journal_title：Nucleic acids research

authors： Taylor NM,Baudin F,von Scheven G,Müller CW

更新日期：2013-10-01 00:00:00

abstract：:Genome browsers are widely used for individually exploring various types of genomic data. A handful of genome browsers offer limited tools for collaboration among multiple users. Here, we describe PBrowse, an integrated real-time collaborative genome browser that enables multiple users to simultaneously view and acces...

journal_title：Nucleic acids research

authors： Szot PS,Yang A,Wang X,Parsania C,Röhm U,Wong KH,Ho JWK

更新日期：2017-05-19 00:00:00

abstract：:We have used in vitro site-directed mutagenesis with synthetic DNA oligonucleotides to introduce single nucleotide mutations in yeast mtDNA. In addition to the expected DNA alterations we also recovered with high frequency mutants with large deletions and insertions which arose through interaction with the synthetic D...

journal_title：Nucleic acids research

authors： Osinga KA,Van der Bliek AM,Van der Horst G,Groot Koerkamp MJ,Tabak HF,Veeneman GH,Van Boom JH

更新日期：1983-12-20 00:00:00

abstract：:The DNA sequence of a 354 basepair EcoRI-HindIII fragment of plasmid pMB9 which has originally been derived from plasmid pSC101 has been resolved. This fragment contains a promoter for transcription directed towards the EcoRI site. Escherichia coli RNA polymerase binds to a region within the EcoRI-HindIII fragment whi...

journal_title：Nucleic acids research

authors： Pannekoek H,Maat J,van den Berg E,Noordermeer I

更新日期：1980-04-11 00:00:00