Abstract：
:Oligonucleotide-directed mutagenesis was used to introduce an A to C transversion at position 523 in the 16S ribosomal RNA gene of Escherichia coli rrnB operon cloned in plasmid pKK3535. E. coli cells transformed with the mutated plasmid were resistant to streptomycin. The mutated ribosomes isolated from these cells were not stimulated by streptomycin to misread the message in a poly(U)-directed assay. They were also restrictive to the stimulation of misreading by other error-promoting related aminoglycoside antibiotics such as neomycin, kanamycin or gentamicin, which do not compete for the streptomycin binding site. The 530 loop where the mutation in the 16S rRNA is located has been mapped at the external surface of the 30S subunit, and is therefore distal from the streptomycin binding site at the subunit interface. Our results support the conclusion that the mutation at position 523 in the 16S rRNA does not interfere with the binding of streptomycin, but prevents the drug from inducing conformational changes in the 530 loop which account for its miscoding effect. Since this effect primarily results from a perturbation of the translational proofreading control, our results also provide evidence that the 530 loop of the 16S rRNA is involved in this accuracy control.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Melançon P,Lemieux C,Brakier-Gingras Ldoi
10.1093/nar/16.20.9631subject
Has Abstractpub_date
1988-10-25 00:00:00pages
9631-9issue
20eissn
0305-1048issn
1362-4962journal_volume
16pub_type
杂志文章abstract：:Point mutations in mitochondrial tRNA genes are responsible for individual subgroups of mitochondrial encephalomyopathies. We have recently reported that point mutations in the tRNA(Leu)(UUR) and tRNA(Lys) genes cause a defect in the normal modification at the first nucleotide of the anticodon. As part of a systematic...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/28.19.3779
更新日期：2000-10-01 00:00:00
abstract：:Enzymes of intermediary metabolism are often reported to have moonlighting functions as RNA-binding proteins and have regulatory roles beyond their primary activities. Human serine hydroxymethyltransferase (SHMT) is essential for the one-carbon metabolism, which sustains growth and proliferation in normal and tumour c...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz129
更新日期：2019-05-07 00:00:00
abstract：:Rapid characterization of unknown biological samples is under the focus of many current studies. Here we report a method for screening of biological samples by optical mapping of their DNA. We use a novel, one-step chemo-enzymatic reaction to covalently bind fluorophores to DNA at the four-base recognition sites of a ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkv563
更新日期：2015-10-15 00:00:00
abstract：:Meiotic cells possess surveillance mechanisms that monitor critical events such as recombination and chromosome synapsis. Meiotic defects resulting from the absence of the synaptonemal complex component Zip1 activate a meiosis-specific checkpoint network resulting in delayed or arrested meiotic progression. Pch2 is an...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw506
更新日期：2016-09-19 00:00:00
abstract：:We have examined the transcript levels of the Saccharomyces cerevisiae DNA repair gene RAD18 in UV irradiated cells, in the mitotic cell cycle, and during meiosis. Levels of RAD18 mRNA increased upon UV irradiation, but remained constant during the mitotic cell cycle. During meiosis, RAD18 mRNA levels rose about 4 fol...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/19.4.893
更新日期：1991-02-25 00:00:00
abstract：:The adenovirus late region 1 (L1) represents an example of an alternatively spliced gene where one 5' splice site is spliced to two alternative 3' splice sites, to produce two mRNAs; the 52,55K and IIIa mRNAs, respectively. Accumulation of the L1 mRNAs is temporally regulated during the infectious cycle. Thus, the pro...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/22.3.332
更新日期：1994-02-11 00:00:00
abstract：:New linker phosphoramidite reagents containing a cleavable 3'-ester linkage are used for attaching the first nucleoside to the surface of a solid- phase support. Inexpensive, underivatized amino supports, such as long chain alkylamine controlled-pore glass, can serve as universal supports. No modifications to phosphor...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkh222
更新日期：2004-01-29 00:00:00
abstract：:Alternative splicing expands the coding capacity of metazoan genes, and it was largely genetic studies in the fruit-fly Drosophila melanogaster that established the principle that regulated alternative splicing results in tissue- and stage-specific protein isoforms with different functions in development. Alternative ...
journal_title：Nucleic acids research
pub_type： 杂志文章,评审
doi：10.1093/nar/gkr648
更新日期：2012-01-01 00:00:00
abstract：:The database of 3D interacting domains (3did, available online for browsing and bulk download at http://3did.irbbarcelona.org) is a catalog of protein-protein interactions for which a high-resolution 3D structure is known. 3did collects and classifies all structural templates of domain-domain interactions in the Prote...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt887
更新日期：2014-01-01 00:00:00
abstract：:alpha2-Macroglobulin covalently linked to poly(L)-lysine can be used as a vehicle for receptor-mediated gene transfer. This modified alpha2-macroglobulin maintains its ability to bind to the alpha2-macroglobulin receptor, and was shown to introduce a luciferase reporter gene plasmid into HepG2 human hepatoma cells in ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/24.19.3873
更新日期：1996-10-01 00:00:00
abstract：:Tight cell-cycle regulation of the histone H4-K20 methyltransferase PR-Set7 is essential for the maintenance of genome integrity. In mammals, this mainly involves the interaction of PR-Set7 with the replication factor PCNA, which triggers the degradation of the enzyme by the CRL4CDT2 E3 ubiquitin ligase. PR-Set7 is al...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky034
更新日期：2018-04-06 00:00:00
abstract：:As proteomics initiatives mature, the need will arise for the multiple visualization of proteins and supramolecular complexes within their true context, in situ. Single-stranded DNA and RNA aptamers can be used for low resolution imaging of cellular receptors and cytoplasmic proteins by light microscopy (LM). These te...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gnh164
更新日期：2004-11-23 00:00:00
abstract：:The universal L-shaped tertiary structure of tRNAs is maintained with the help of nucleotide modifications within the D- and T-loops, and these modifications are most extensive within hyperthermophilic species. The obligate-commensal Nanoarchaeum equitans and its phylogenetically-distinct host Ignicoccus hospitalis gr...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa411
更新日期：2020-07-09 00:00:00
abstract：:Alternative splicing of pre-mRNA generates protein diversity. Dysfunction of splicing machinery and expression of specific transcripts has been linked to cancer progression and drug response. Exon microarray technology enables genome-wide quantification of expression levels of the majority of exons and facilitates the...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkr513
更新日期：2011-10-01 00:00:00
abstract：:We have isolated the 5'-end of the gene for the rat and human link protein by screening genomic libraries with oligonucleotides corresponding to the 5'-cDNA sequence. Several overlapping clones were isolated for the human link protein gene, while only one clone was obtained for the rat. All the clones contained a sing...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/19.8.1933
更新日期：1991-04-25 00:00:00
abstract：:The translational regulation of ferritin expression currently represents the only well characterized example for eukaryotic translational control by high affinity interactions between a specific cytoplasmic protein, iron regulatory factor [IRF], and an mRNA-binding site, the iron-responsive element [IRE], located in t...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/20.21.5555
更新日期：1992-11-11 00:00:00
abstract：:Alpha-1-microglobulin and bikunin are two plasma glycoproteins encoded by an alpha-1-microglobulin/bikunin precursor (AMBP) gene. The strict liver-specific expression of the AMBP gene is controlled by a potent enhancer made of six clustered boxes numbered 1-6 that have been reported to be proven or potential binding s...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/23.3.395
更新日期：1995-02-11 00:00:00
abstract：:The high-mobility-group A2 protein (HMGA2) plays important functional roles in transcriptional regulation, DNA replication and chromatin structure. In this study, the effect of histone deacetylase inhibition on the transcriptional activity of the Hmga2 gene was investigated in vivo both at the endogenous gene level an...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkg403
更新日期：2003-06-15 00:00:00
abstract：:For over 10 years, Binding MOAD (Mother of All Databases; http://www.BindingMOAD.org) has been one of the largest resources for high-quality protein-ligand complexes and associated binding affinity data. Binding MOAD has grown at the rate of 1994 complexes per year, on average. Currently, it contains 23,269 complexes ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gku1088
更新日期：2015-01-01 00:00:00
abstract：:Understanding the molecular mechanisms that influence transposable element target site preferences is a fundamental challenge in functional and evolutionary genomics. Large-scale transposon insertion projects provide excellent material to study target site preferences in the absence of confounding effects of post-inse...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn563
更新日期：2008-11-01 00:00:00
abstract：:Kinetically monitored, reverse transcriptase-initiated PCR (kinetic RT-PCR, kRT-PCR) is a novel application of kinetic PCR for high throughput transcript quantitation in total cellular RNA. The assay offers the simplicity and flexibility of an enzyme assay with distinct advantages over DNA microarray hybridization and...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/28.2.e2
更新日期：2000-01-15 00:00:00
abstract：:The non-essential MGS1 gene of Saccharomyces cerevisiae is highly conserved in eukaryotes and encodes an enzyme containing both DNA-dependent ATPase and DNA annealing activities. MGS1 appears to function in post-replicational repair processes that contribute to genome stability. In this study, we identified MGS1 as a ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gki900
更新日期：2005-10-26 00:00:00
abstract：:DNA sequences, that control expression of the spliced leader (SL) RNA gene in the parasitic protozoan Leishmania amazonensis, were mapped by block substitution mutagenesis. In the absence of a functional in vitro system for transcription, no promoter elements have yet been identified in this organism. We therefore dev...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/22.11.1959
更新日期：1994-06-11 00:00:00
abstract：:The triphosphate of the nucleoside deoxyribosyl dihydropyrimido[4,5-c][1,2]oxazin-7-one (dP) is known to be incorporated into DNA efficiently by Taq polymerase and is a useful tool for polymerase-mediated in vitro mutagenesis. It is shown here that dP is a potent mutagen in Escherichia coli and Salmonella typhimurium ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/25.8.1548
更新日期：1997-04-15 00:00:00
abstract：:IHF and HU are two heterodimeric nucleoid-associated proteins (NAP) that belong to the same protein family but interact differently with the DNA. IHF is a sequence-specific DNA-binding protein that bends the DNA by over 160°. HU is the most conserved NAP, which binds non-specifically to duplex DNA with a particular pr...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkr1236
更新日期：2012-04-01 00:00:00
abstract：:Inability of T7 RNA polymerase to processively transcribe higher eukaryotic chromatin is interpreted as a correlate of its reported inhibition by nucleosomes on reconstituted templates in vitro . We used chromosomally integrated reporter cassettes to examine features of T7 transcription in a lower eukaryotic system. L...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/26.20.4626
更新日期：1998-10-15 00:00:00
abstract：:OMA (Orthologous MAtrix) is a database that identifies orthologs among publicly available, complete genomes. Initiated in 2004, the project is at its 11th release. It now includes 1000 genomes, making it one of the largest resources of its kind. Here, we describe recent developments in terms of species covered; the al...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq1238
更新日期：2011-01-01 00:00:00
abstract：:In this paper, we describe a new method for selecting RNA aptamers that cooperatively bind to two specific sites within a target RNA. We designed a selection system in which two RNAs, a target RNA and a RNA pool, were assembled by employing a pre-organized GAAA tetraloop-11-nt receptor interaction. This allows us to s...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn1012
更新日期：2009-02-01 00:00:00
abstract：:A new method for specific reamplification of DDRT-PCR products is presented. After transient ligation of the primary DDRT-PCR fragments into a T-vector, the cDNAs of interest were reamplified by hemi-nested PCR and thermally asymmetric cycles. In contrast to the originally described protocol, this method of reamplific...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/26.4.1130
更新日期：1998-02-15 00:00:00
abstract：:Virtually the whole region of the mobile dispersed genetic element mdg1 including the direct repeats that frame mdg1 /1/ is transcribed. The end of the transcriptional unit is located within the direct repeat. Although the transcription of mdg1 is symmetric, one direction is transcribed preferentially and is a full-le...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/8.22.5347
更新日期：1980-11-25 00:00:00
