Abstract:
:alpha2-Macroglobulin covalently linked to poly(L)-lysine can be used as a vehicle for receptor-mediated gene transfer. This modified alpha2-macroglobulin maintains its ability to bind to the alpha2-macroglobulin receptor, and was shown to introduce a luciferase reporter gene plasmid into HepG2 human hepatoma cells in vitro. The alpha2-macroglobulin receptor is a very large and multifunctional cell surface receptor, whose rapid and efficient internalization rate makes it attractive for gene therapy, e.g. for hepatic gene targeting via injection into the portal vein.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Schneider H,Huse K,Birkenmeier G,Otto A,Scholz GHdoi
10.1093/nar/24.19.3873subject
Has Abstractpub_date
1996-10-01 00:00:00pages
3873-4issue
19eissn
0305-1048issn
1362-4962pii
l60175journal_volume
24pub_type
杂志文章abstract::One of the greatest challenges that modern molecular biology is facing is the understanding of the complex mechanisms regulating gene expression. A fundamental step in this process requires the characterization of regulatory motifs playing key roles in the regulation of gene expression at transcriptional and post-tran...
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journal_title:Nucleic acids research
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