Myh7b/miR-499 gene expression is transcriptionally regulated by MRFs and Eos.

abstract：:For the analysis of protein-DNA interactions by coupled gel-shift/footprinting, DNA fragments need to be extracted from polyacrylamide gels and subsequently separated on high resolution gels. Due to impurities in the extracted DNA, single nucleotide resolution is frequently not achieved. We now describe an improved ex...

journal_title：Nucleic acids research

authors： Ragnhildstveit E,Fjose A,Becker PB,Quivy JP

更新日期：1997-01-15 00:00:00

abstract：:Molecules of single-stranded ribosomal RNA and double-stranded replicative form of phage f2 RNA (dsRNA) adopt a compact form in solutions, containing sufficiently high concentrations of salt (NaCl) and polymer (PEG). However, only in the cases of native dsRNA molecules the compact particles are characterized by a regu...

journal_title：Nucleic acids research

authors： Evdokimov YM,Pyatigorskaya TL,Kadikov VA,Polyvtsev OF,Doskocil J,Koudelka J,Varshavsky YM

更新日期：1976-06-01 00:00:00

abstract：:Toscana virus (TOSV) is an arthropod-borne human pathogen responsible for seasonal outbreaks of fever and meningoencephalitis in the Mediterranean basin. TOSV is a segmented negative-strand RNA virus (sNSV) that belongs to the genus phlebovirus (family Phenuiviridae, order Bunyavirales), encompassing other important h...

journal_title：Nucleic acids research

authors： Jones R,Lessoued S,Meier K,Devignot S,Barata-García S,Mate M,Bragagnolo G,Weber F,Rosenthal M,Reguera J

更新日期：2019-11-18 00:00:00

abstract：:It is informative to detect highly conserved positions in proteins and nucleic acid sequence/structure since they are often indicative of structural and/or functional importance. ConSurf (http://consurf.tau.ac.il) and ConSeq (http://conseq.tau.ac.il) are two well-established web servers for calculating the evolutionar...

journal_title：Nucleic acids research

authors： Ashkenazy H,Erez E,Martz E,Pupko T,Ben-Tal N

更新日期：2010-07-01 00:00:00

abstract：:The binding of nine aminoalkyl adenylates to isoleucyl-tRNA synthetase from Escherichia coli MRE 600 was measured and compared with the binding of the cognate amino acids. It was found that they bind rather tightly to the enzyme, the Kd's ranging from 3.1.10(-4) M with glycinol-AMP ester to 3.7.10(-9) M with L-isoleuc...

journal_title：Nucleic acids research

authors： Flossdorf J,Marutzky R,Messer K,Kula MR

更新日期：1977-03-01 00:00:00

abstract：:DrugCentral (http://drugcentral.org) is an open-access online drug compendium. DrugCentral integrates structure, bioactivity, regulatory, pharmacologic actions and indications for active pharmaceutical ingredients approved by FDA and other regulatory agencies. Monitoring of regulatory agencies for new drugs approvals ...

journal_title：Nucleic acids research

authors： Ursu O,Holmes J,Knockel J,Bologa CG,Yang JJ,Mathias SL,Nelson SJ,Oprea TI

更新日期：2017-01-04 00:00:00

abstract：:ANISEED (www.aniseed.cnrs.fr) is the main model organism database for tunicates, the sister-group of vertebrates. This release gives access to annotated genomes, gene expression patterns, and anatomical descriptions for nine ascidian species. It provides increased integration with external molecular and taxonomy datab...

journal_title：Nucleic acids research

authors： Brozovic M,Dantec C,Dardaillon J,Dauga D,Faure E,Gineste M,Louis A,Naville M,Nitta KR,Piette J,Reeves W,Scornavacca C,Simion P,Vincentelli R,Bellec M,Aicha SB,Fagotto M,Guéroult-Bellone M,Haeussler M,Jacox E,Lowe

更新日期：2018-01-04 00:00:00

abstract：:The SeqA protein binds hemi-methylated GATC sites and forms structures that sequester newly replicated origins and trail the replication forks. Cells that lack SeqA display signs of replication fork disintegration. The broken forks could arise because of over-initiation (the launching of too many forks) or lack of dyn...

journal_title：Nucleic acids research

authors： Pedersen IB,Helgesen E,Flåtten I,Fossum-Raunehaug S,Skarstad K

更新日期：2017-06-20 00:00:00

abstract：:We have developed an effective post-PCR sterilization process and have applied the procedure to a diagnostic assay for HIV-1. The method, which is based on isopsoralen photochemistry, satisfies both the inactivation and hybridization requirements of a practical sterilization process. The key feature of the technique i...

journal_title：Nucleic acids research

authors： Isaacs ST,Tessman JW,Metchette KC,Hearst JE,Cimino GD

更新日期：1991-01-11 00:00:00

abstract：:The products of many bacterial non-ribosomal peptide synthetases (NRPS) are highly important secondary metabolites, including vancomycin and other antibiotics. The ability to predict substrate specificity of newly detected NRPS Adenylation (A-) domains by genome sequencing efforts is of great importance to identify an...

journal_title：Nucleic acids research

authors： Röttig M,Medema MH,Blin K,Weber T,Rausch C,Kohlbacher O

更新日期：2011-07-01 00:00:00

abstract：:GC content bias describes the dependence between fragment count (read coverage) and GC content found in Illumina sequencing data. This bias can dominate the signal of interest for analyses that focus on measuring fragment abundance within a genome, such as copy number estimation (DNA-seq). The bias is not consistent b...

journal_title：Nucleic acids research

authors： Benjamini Y,Speed TP

更新日期：2012-05-01 00:00:00

abstract：:During DNA replication, the presence of 8-oxoguanine (8-oxoG) lesions in the template strand cause the high-fidelity (HiFi) DNA polymerase (Pol) to stall. An early response to 8-oxoG lesions involves 'on-the-fly' translesion synthesis (TLS), in which a specialized TLS Pol is recruited and replaces the stalled HiFi Pol...

journal_title：Nucleic acids research

authors： Cranford MT,Kaszubowski JD,Trakselis MA

更新日期：2020-11-04 00:00:00

abstract：:PUF proteins, named for Drosophila Pumilio (PUM) and Caenorhabditis elegans fem-3-binding factor (FBF), recognize specific sequences in the mRNAs they bind and control. RNA binding by classical PUF proteins is mediated by a characteristic PUM homology domain (PUM-HD). The Puf1 and Puf2 proteins possess a distinct arch...

journal_title：Nucleic acids research

authors： Qiu C,Dutcher RC,Porter DF,Arava Y,Wickens M,Hall TMT

更新日期：2019-09-19 00:00:00

abstract：:Base compositions were examined at every position in codons of more than 50 genes from taxonomically different bacteria and of the corresponding antisense sequences on the bacterial genes. We propose that the nonstop frame on antisense strand [NSF(a)] of GC-rich bacterial genes is the most promising sequence for newly...

journal_title：Nucleic acids research

authors： Ikehara K,Amada F,Yoshida S,Mikata Y,Tanaka A

更新日期：1996-11-01 00:00:00

abstract：:We have determined the complete nucleotide sequence of the regions of mouse ribosomal RNA transcription unit which separate mature rRNA genes. These internal transcribed spacers (ITS) are excised from rRNA precursor during ribosome biosynthesis. ITS 1, between 18S and 5.8S rRNA genes, is 999 nucleotides long. ITS 2, b...

journal_title：Nucleic acids research

authors： Michot B,Bachellerie JP,Raynal F

更新日期：1983-05-25 00:00:00

abstract：:Gene II protein is the only phage-encoded protein required for the double-stranded DNA replication of the distantly related filamentous phages IKe and Ff (M13, fd and f1). Complementation studies have demonstrated that, despite a significant degree of homology between the nucleotide sequences of the gene II's of IKe a...

journal_title：Nucleic acids research

authors： Peeters BP,Schoenmakers JG,Konings RN

更新日期：1986-06-25 00:00:00

abstract：:Cdc48/p97 is an evolutionary conserved ubiquitin-dependent chaperone involved in a broad array of cellular functions due to its ability to associate with multiple cofactors. Aside from its role in removing RNA polymerase II from chromatin after DNA damage, little is known about how this AAA-ATPase is involved in the t...

journal_title：Nucleic acids research

authors： Bonizec M,Hérissant L,Pokrzywa W,Geng F,Wenzel S,Howard GC,Rodriguez P,Krause S,Tansey WP,Hoppe T,Dargemont C

更新日期：2014-01-01 00:00:00

abstract：:RNA polymerase (RNAP) is a major target of gene regulation. Thermus thermophilus bacteriophage P23-45 encodes two RNAP binding proteins, gp39 and gp76, which shut off host gene transcription while allowing orderly transcription of phage genes. We previously reported the structure of the T. thermophilus RNAP•σA holoenz...

journal_title：Nucleic acids research

authors： Ooi WY,Murayama Y,Mekler V,Minakhin L,Severinov K,Yokoyama S,Sekine SI

更新日期：2018-01-09 00:00:00

abstract：:Allele-specific DNA methylation, histone acetylation and histone methylation are recognized as epigenetic characteristics of imprinted genes and imprinting centers (ICs). These epigenetic modifications are also used to regulate tissue-specific gene expression. Epigenetic differences between alleles can be significant ...

journal_title：Nucleic acids research

authors： Lau JC,Hanel ML,Wevrick R

更新日期：2004-06-24 00:00:00

abstract：:Unwanted side effects of drugs are a burden on patients and a severe impediment in the development of new drugs. At the same time, adverse drug reactions (ADRs) recorded during clinical trials are an important source of human phenotypic data. It is therefore essential to combine data on drugs, targets and side effects...

journal_title：Nucleic acids research

authors： Kuhn M,Letunic I,Jensen LJ,Bork P

更新日期：2016-01-04 00:00:00

abstract：:The resonance Raman spectrum of native DNA has been obtained using excitation at 257 nm. In a first part, the spectral lines are assigned to the different nucleotide bases which provide the resonance effect. In a second part, the interactions of DNA with basic peptides (Arginine Methylester, Lysine Methylester, Arginy...

journal_title：Nucleic acids research

authors： Laigle A,Chinsky L,Turpin PY

更新日期：1982-03-11 00:00:00

abstract：:Antisense oligodeoxynucleotides (ODNs) have biological activity in treating various forms of cancer. The antisense effects of two types of 20mer ODNs, phosphorothioate-modified ODNs (S-ODNs) and S-ODNs with 12 2'-O-methyl groups (Me-S-ODNs), targeted to sites 109 and 277 of bcl-2 mRNA, were compared. Both types were a...

journal_title：Nucleic acids research

authors： Yoo BH,Bochkareva E,Bochkarev A,Mou TC,Gray DM

更新日期：2004-04-02 00:00:00

abstract：:Atomistic molecular dynamics (MD) simulations generate a wealth of information related to the dynamics of proteins. If properly analyzed, this information can lead to new insights regarding protein function and assist wet-lab experiments. Aiming to identify interactions between individual amino acid residues and the r...

journal_title：Nucleic acids research

authors： Serçinoglu O,Ozbek P

更新日期：2018-07-02 00:00:00

abstract：:One surprisingly common element of RNA secondary structure consists of a hairpin capped by a four-base loop (or the tetraloop). Recently the 3-D structures of two RNA-tetraloops have been determined by NMR-studies. Both structures have a similar architecture: the first and the last bases of the loop form a hydrogen bo...

journal_title：Nucleic acids research

authors： Kajava A,Rüterjans H

更新日期：1993-09-25 00:00:00

abstract：:The bacterial transcription terminator, Rho, terminates transcription at half of the operons. According to the classical model derived from in vitro assays on a few terminators, Rho is recruited to the transcription elongation complex (EC) by recognizing specific sites (rut) on the nascent RNA. Here, we explored the m...

journal_title：Nucleic acids research

authors： Shashni R,Qayyum MZ,Vishalini V,Dey D,Sen R

更新日期：2014-09-01 00:00:00

abstract：:Many, if not all, mRNAs in T.brucei start with the same sequence of 35 nucleotides, separately encoded in clustered so-called mini-exon repeats. From these mini-exon repeats a 141-nt precursor RNA with the 35-nt sequence at its 5' end is transcribed. Indirect evidence suggests that this RNA is linked in a second step ...

journal_title：Nucleic acids research

authors： Kooter JM,Borst P

更新日期：1984-12-21 00:00:00

abstract：:Elongation factor P (EF-P) is a conserved ribosome-binding protein that structurally mimics tRNA to enable the synthesis of peptides containing motifs that otherwise would induce translational stalling, including polyproline. In many bacteria, EF-P function requires post-translational modification with (R)-β-lysine by...

journal_title：Nucleic acids research

authors： Katz A,Solden L,Zou SB,Navarre WW,Ibba M

更新日期：2014-03-01 00:00:00

abstract：:We found previously that the Chlamydomonas HSP70A promoter counteracts transcriptional silencing of downstream promoters in a transgene setting. To elucidate the underlying mechanisms, we analyzed chromatin state and transgene expression in transformants containing HSP70A-RBCS2-ble (AR-ble) constructs harboring deleti...

journal_title：Nucleic acids research

authors： Strenkert D,Schmollinger S,Schroda M

更新日期：2013-05-01 00:00:00

abstract：:Alu elements have repeatedly been found involved in gene rearrangements in humans. Although these elements have been suggested to stimulate gene rearrangements, sparse information is available for the possible mechanism(s) of these events. Here we present a compilation of Alu elements that have been involved in recomb...

journal_title：Nucleic acids research

authors： Rüdiger NS,Gregersen N,Kielland-Brandt MC

更新日期：1995-01-25 00:00:00

abstract：:Stable protein complexes, including those formed with RNA, are major building blocks of every living cell. Escherichia coli has been the leading bacterial organism with respect to global protein-protein networks. Yet, there has been no global census of RNA/protein complexes in this model species of microbiology. Here,...

journal_title：Nucleic acids research

authors： Hör J,Di Giorgio S,Gerovac M,Venturini E,Förstner KU,Vogel J

更新日期：2020-09-18 00:00:00