Abstract:
:YY1 is ubiquitously expressed zinc finger DNA binding protein. It can act as a transcriptional repressor or activator and, when binding at the initiator element, as a component of the basal transcription complex. Binding sites for YY1 have been reported in a wide variety of promoters and they exhibit substantial diversity in their sequence. To better understand how YY1 interacts with DNA and to be able to predict the presence of YY1 sites in a more comprehensive fashion, we have selected YY1 binding sites from a random pool of oligonucleotides. The sites display considerable heterogeneity, but contain a conserved 5'-CAT-3' core flanked by variable regions, generating the consensus 5'-(C/g/a)(G/t)(C/t/a)CATN(T/a)(T/g/c)-3', where the upper case letters represent the preferred base. This high degree of flexibility in DNA recognition can be predicted by modeling the interaction of the four YY1 zinc fingers with DNA and a detailed model for this interaction is presented and discussed.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Hyde-DeRuyscher RP,Jennings E,Shenk Tdoi
10.1093/nar/23.21.4457subject
Has Abstractpub_date
1995-11-11 00:00:00pages
4457-65issue
21eissn
0305-1048issn
1362-4962pii
5g0386journal_volume
23pub_type
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