Abstract：
:The synthesis of cyclic di-, tri-, tetra-, penta- and hexa-thymidylic acids (16; n = 2, 3, 4, 5 and 6) and the cyclic hexadeoxyribonucleotide [d(CpCpTpApGpGp)], via the "filtration" approach, is reported. Some of the physical properties of the cyclic oligonucleotides are discussed, and their susceptibility to digestion in the presence of phosphorylytic enzymes has been studied.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Rao MV,Reese CBdoi
10.1093/nar/17.20.8221subject
Has Abstractpub_date
1989-10-25 00:00:00pages
8221-39issue
20eissn
0305-1048issn
1362-4962journal_volume
17pub_type
杂志文章abstract：:The tissue-specific expression of the Drosophila beta 2 tubulin gene ( B2t ) is accomplished by the action of a 14-bp activator element (beta2UE1) in combination with certain regulatory elements of the TATA-less, Inr-containing B2t core promoter. We performed an in vivo analysis of the Inr element function in the B2t ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/28.6.1439
更新日期：2000-03-15 00:00:00
abstract：:PIECE (Plant Intron Exon Comparison and Evolution) is a web-accessible database that houses intron and exon information of plant genes. PIECE serves as a resource for biologists interested in comparing intron-exon organization and provides valuable insights into the evolution of gene structure in plant genomes. Recent...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw935
更新日期：2017-01-04 00:00:00
abstract：:In fission yeast and other eukaryotes, Rec12 (Spo11) is thought to catalyze the formation of dsDNA breaks (DSBs) that initiate homologous recombination in meiosis. Rec12 is orthologous to the catalytic subunit of topoisomerase VI (Top6A). Guided by the crystal structure of Top6A, we engineered the rec12 locus to encod...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq993
更新日期：2011-03-01 00:00:00
abstract：:The integrases are a diverse family of tyrosine recombinases which rearrange DNA duplexes by means of conservative site-specific recombination reactions. Members of this family, of which the well-studied lambda Int protein is the prototype, were previously found to share four strongly conserved residues, including an ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/25.18.3605
更新日期：1997-09-15 00:00:00
abstract：:Chromatin organization and epigenetic markers influence splicing, though the magnitudes of these effects and the mechanisms are largely unknown. Here, we demonstrate that linker histone H1.5 influences mRNA splicing. We observed that linker histone H1.5 binds DNA over splice sites of short exons in human lung fibrobla...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz338
更新日期：2019-07-09 00:00:00
abstract：:AvrBs3, the founding member of the Xanthomonas transcription-activator-like effectors (TALEs), is translocated into the plant cell where it localizes to the nucleus and acts as transcription factor. The DNA-binding domain of AvrBs3 consists of 17.5 nearly-identical 34 amino acid-repeats. Each repeat specifies binding ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gku341
更新日期：2014-06-01 00:00:00
abstract：:Chromatin footprinting in Drosophila tissue culture cells has detected the binding of a non-histone protein at +8 of the distal Adh RNA start site, on a 10-bp direct repeat motif abutting a nucleosome positioned over the inactive Adh distal promoter. Alternatively the active promoter is bound by a transcription initia...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/20.17.4481
更新日期：1992-09-11 00:00:00
abstract：:The repAC operon of plasmid RSF1010 consists of the genes for proteins E, F, RepA (DNA helicase), and RepC (origin-binding initiator protein) and is transcriptionally initiated by a promoter called P4. We have studied the expression of the repAC operon in vivo by using fusions to the lacZ reporter gene. The results sh...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/18.21.6215
更新日期：1990-11-11 00:00:00
abstract：:The abundance of Alu RNA is transiently increased by heat shock in human cell lines. This effect is specific to Alu repeats among Pol III transcribed genes, since the abundance of 7SL, 7SK, 5S and U6 RNAs is essentially unaffected by heat shock. The rapid induction of Alu expression precedes the heat shock induction o...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/23.10.1758
更新日期：1995-05-25 00:00:00
abstract：:The 'regions' method for multisequence alignment used in the previously reported program MALIGN has been generalized to include recursive refinement so that unaligned portions between two regions at the current level of resolution can be handled with increased resolution. Additionally, there is incorporated a limiting...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/16.5.1683
更新日期：1988-03-11 00:00:00
abstract：:The single nucleotide polymorphisms (SNPs) in conserved protein regions have been thought to be strong candidates that alter protein functions. Thus, we have developed SNP@Domain, a web resource, to identify SNPs within human protein domains. We annotated SNPs from dbSNP with protein structure-based as well as sequenc...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkl323
更新日期：2006-07-01 00:00:00
abstract：:Several lines of evidence indicate that differences in DNA repair capacity are an important source of variability in cancer risk. However, traditional assays for measurement of DNA repair activity in human samples are laborious and time-consuming. DNA glycosylases are the first step in base excision repair of a variet...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gni166
更新日期：2005-10-21 00:00:00
abstract：:Not I linking clones contain sequences flanking Not I recognition sites and were previously shown to be tightly associated with CpG islands and genes. To directly assess the value of Not I clones in genome research, high density grids with 50 000 Not I linking clones originating from six representative Not I linking l...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/28.7.1635
更新日期：2000-04-01 00:00:00
abstract：:Nucleosomes are the most abundant protein-DNA complexes in eukaryotes that provide compaction of genomic DNA and are implicated in regulation of transcription, DNA replication and repair. The details of DNA positioning on the nucleosome and the DNA conformation can provide key regulatory signals. Hydroxyl-radical foot...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx616
更新日期：2017-09-19 00:00:00
abstract：:LocDB is a manually curated database with experimental annotations for the subcellular localizations of proteins in Homo sapiens (HS, human) and Arabidopsis thaliana (AT, thale cress). Currently, it contains entries for 19,604 UniProt proteins (HS: 13,342; AT: 6262). Each database entry contains the experimentally der...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq927
更新日期：2011-01-01 00:00:00
abstract：:We recently reported that the antitumor triazoloacridone, compound C-1305, is a topoisomerase II poison with unusual properties. In this study we characterize the DNA interactions of C-1305 in vitro, in comparison with other topoisomerase II inhibitors. Our results show that C-1305 binds to DNA by intercalation and po...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gki904
更新日期：2005-10-27 00:00:00
abstract：:We previously showed that mRNAs synthesized from three genes that naturally lack introns contain a portion of their coding sequence, known as a cytoplasmic accumulation region (CAR), which is essential for stable accumulation of the intronless mRNAs in the cytoplasm. The CAR in each mRNA is unexpectedly large, ranging...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks1314
更新日期：2013-02-01 00:00:00
abstract：:We have previously isolated two different aFGF cDNA clones from kidney and brain. The two corresponding mRNA, designated aFGF 1.A and 1.B, are the predominant species in kidney and brain, respectively. During the characterization of aFGF mRNA in glioblastoma cells, we demonstrated that aFGF mRNA in U1242MG and D65MG g...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/21.3.489
更新日期：1993-02-11 00:00:00
abstract：:Alternative splicing generates multiple transcript and protein isoforms from a single gene and controls transcript intracellular localization and stability by coupling to mRNA export and nonsense-mediated mRNA decay (NMD). RNA interference (RNAi) is a potent mechanism to modulate gene expression. However, its interact...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa1260
更新日期：2021-01-25 00:00:00
abstract：:Eukaryotic genomes contain many endogenous retroviral sequences (ERVs). ERVs are often severely mutated, therefore difficult to detect. A platform independent (Java) program package, RetroTector (ReTe), was constructed. It has three basic modules: (i) detection of candidate long terminal repeats (LTRs), (ii) detection...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm515
更新日期：2007-01-01 00:00:00
abstract：:Transcription initiation is a major step in gene regulation for all organisms. In bacteria, the promoter DNA is first recognized by RNA polymerase (RNAP) to yield an initial closed complex. This complex subsequently undergoes conformational changes resulting in DNA strand separation to form a transcription bubble and ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx1146
更新日期：2018-01-25 00:00:00
abstract：:Recent evidence indicates a link between Parkinson's Disease (PD) and the expression of a-synuclein (SNCA) isoforms with different 3' untranslated regions (3'UTRs). Yet, the post-transcriptional mechanisms regulating SNCA expression are unknown. Using a large-scale in vitro /in silico screening we identified RNA-bindi...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx1048
更新日期：2017-12-15 00:00:00
abstract：:Meiotic cells possess surveillance mechanisms that monitor critical events such as recombination and chromosome synapsis. Meiotic defects resulting from the absence of the synaptonemal complex component Zip1 activate a meiosis-specific checkpoint network resulting in delayed or arrested meiotic progression. Pch2 is an...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw506
更新日期：2016-09-19 00:00:00
abstract：:Single nucleotide polymorphisms (SNPs) are, together with copy number variation, the primary source of variation in the human genome. SNPs are associated with altered response to drug treatment, susceptibility to disease and other phenotypic variation. Furthermore, during genetic screens for disease-associated mutatio...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm979
更新日期：2008-01-01 00:00:00
abstract：:We have used in vitro transcription in isolated pea nuclei to determine whether the expression of genes for the seed storage proteins legumin and vicilin is regulated transcriptionally or post-transcriptionally in response to changing sulfur status in the developing seed. During the first 48 h of recovery from S defic...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/13.3.999
更新日期：1985-02-11 00:00:00
abstract：:The study of pathogenic mitochondrial DNA mutations has, in most cases, relied on the production of transmitochondrial cybrids. Although the procedure to produce such cybrids is well established, it is laborious and cumbersome. Moreover, the mechanical enucleation procedure is inefficient and different techniques have...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gng100
更新日期：2003-08-15 00:00:00
abstract：:Phenotype analysis is commonly recognized to be of great importance for gaining insight into genetic interaction underlying inherited diseases. However, few computational contributions have been proposed for this purpose, mainly owing to lack of controlled clinical information easily accessible and structured for comp...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gki454
更新日期：2005-07-01 00:00:00
abstract：:We have previously reported that transcription from a distal promoter (promoter B) of the estrogen receptor alpha (ERalpha) gene is responsible for the increased expression of ERalpha in human breast carcinomas. This paper first characterized the promoter B region in terms of transient transfection experiments with lu...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/27.3.903
更新日期：1999-02-01 00:00:00
abstract：:To unravel the network of transcription factors established during development it is important to understand how genes specifically expressed during embryogenesis are regulated. Oct-4 is a transcription factor whose expression is associated with an undifferentiated cell phenotype in the early mouse embryo and is downr...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/22.6.901
更新日期：1994-03-25 00:00:00
abstract：:Human transcription factor IIIC contains a 55 kDa polypeptide which specifically interacts with the Adenovirus 2 VAI gene promoter and which mimics most of the DNA binding properties of the entire factor. The specificity and affinity of this protein:DNA interaction was demonstrated by: (i) Separation of purified fract...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/18.16.4743
更新日期：1990-08-25 00:00:00
