Abstract：
:Primase synthesizes decaribonucleotides for priming of lagging and possibly leading strand synthesis at a replication fork. The sites of initiation by purified mouse primase were shown to be highly specific within the SV40 origin of replication. This study further examines the role of the 27-bp inverted repeat in the origin for initiation. A site is observed on the L-strand template at nucleotide position (np) 22 positioned a similar distance from the 27-bp inverted repeat as sites previously reported on the E-strand. The initiations adjacent to the 27-bp repeat have a higher Km for rATP than other sites. A deletion within the inverted repeat eliminated initiation at sites proximal to the hairpin on both E and L strands but had no effect at more distant sites. A deletion mutant which left the inverted repeat intact but deleted the initiation sites at np 5210-5220 on the E-strand was not active as a template for proximal sites. These results indicate that primase has two modes of recognition, one that requires the SV40 inverted repeat structure and a specific sequence and another that requires sequence alone. Additional regions of the SV40 genome have also been examined and of approximately 2000 nucleotides of single stranded template examined, only one additional site was observed at np 2412 on the E-strand. This indicates that primase initiations are highly specific for the SV40 origin and their potential functional role is discussed.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Tseng BY,Prussak CEdoi
10.1093/nar/17.5.1953subject
Has Abstractpub_date
1989-03-11 00:00:00pages
1953-63issue
5eissn
0305-1048issn
1362-4962journal_volume
17pub_type
杂志文章abstract：:The design and synthesis of metal complexes that can specifically target DNA secondary structure has attracted considerable attention. Chiral metallosupramolecular complexes (e.g. helicates) in particular display unique DNA-binding behavior, however until recently few examples which are both water-compatible and enant...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx244
更新日期：2017-05-19 00:00:00
abstract：:RNA processing is critical for proper spatial and temporal control of gene expression. The ubiquitous nuclear polyadenosine RNA binding protein, PABPN1, post-transcriptionally regulates multiple steps of gene expression. Mutations in the PABPN1 gene expanding an N-terminal alanine tract in the PABPN1 protein from 10 a...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky535
更新日期：2018-09-06 00:00:00
abstract：:During erythropoiesis in the chick, the nucleosome repeat length increases from 190 base pairs to 212 base pairs. This increase is correlated with a dramatic increase in the concentration of the red cell specific histone H5 (from 0.2 molecules per nucleosome to 1 molecule per nucleosome) and with no change in the conc...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/5.4.1179
更新日期：1978-04-01 00:00:00
abstract：:In the present study, the relationship between short interfering RNA (siRNA) sequence and RNA interference (RNAi) effect was extensively analyzed using 62 targets of four exogenous and two endogenous genes and three mammalian and Drosophila cells. We present the rules that may govern siRNA sequence preference and in a...
journal_title：Nucleic acids research
pub_type： 指南,杂志文章
doi：10.1093/nar/gkh247
更新日期：2004-02-09 00:00:00
abstract：:Adaptation to the host cell environment to efficiently take-over the host cell's machinery is crucial in particular for small RNA viruses like picornaviruses that come with only small RNA genomes and replicate exclusively in the cytosol. Their Internal Ribosome Entry Site (IRES) elements are specific RNA structures th...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks182
更新日期：2012-07-01 00:00:00
abstract：:The nucleotide sequence of a 981 bp's HincII-PvuII DNA fragment containing the 3' end of the malEFG operon in E. coli was determined. This sequence displayed a putative Rho-independent transcription termination site localized 87 bp's after the stop codon of malG. When cloned into plasmid pKG1800, the HincII-PvuII frag...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/16.9.4097
更新日期：1988-05-11 00:00:00
abstract：:The nucleotide sequence of 5.8S rRNA from the Chinese silkworm Philosamia cynthia ricini has been determined by gel sequencing and mobility shift methods. The complete primary structure is (sequence in text). This is one of the largest known 5.8S rRNAs. As compared to Bombyx 5.8S rRNA, it is two nucleotides longer; t...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/10.20.6383
更新日期：1982-10-25 00:00:00
abstract：:Besides the nicking-closing (topoisomerase I) activity, an ATP-dependent DNA topoisomerase is present in rat liver nuclei. The enzyme, partially purified, is able to catenate in vitro closed DNA circles in a magnesium-dependent, ATP-dependent, histone H1-dependent reaction, and to decatenate in vitro kinetoplast DNA n...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/11.4.1059
更新日期：1983-02-25 00:00:00
abstract：:In continuation with work regarding the evaluation of the energy of association of various RNA bases with various base pairs, the results of the computations of the electrostatic interaction energy of A-U base pair with four RNA bases viz. Adenine, Uracil, Guanine and Cytosine have been reported. Non bonded induced po...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/8.17.3983
更新日期：1980-09-11 00:00:00
abstract：:Fourteen P-element insertion mutants at the yellow (y) gene of D. melanogaster have been analysed by restriction mapping and DNA sequencing. The distribution of the insertion sites is nonrandom, with a preferred target site in the 5'-transcribed but non-translated leader of the gene. Transcription analysis indicates t...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/16.7.3039
更新日期：1988-04-11 00:00:00
abstract：:Lens epithelium-derived growth factor/p75 (LEDGF/p75) is a transcriptional coactivator involved in stress response, autoimmune disease, cancer and HIV replication. A fusion between the nuclear pore protein NUP98 and LEDGF/p75 has been found in human acute and chronic myeloid leukemia and association of LEDGF/p75 with ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq410
更新日期：2010-10-01 00:00:00
abstract：:We present version 2 of the SPINE system for structural proteomics. SPINE is available over the web at http://nesg.org. It serves as the central hub for the Northeast Structural Genomics Consortium, allowing collaborative structural proteomics to be carried out in a distributed fashion. The core of SPINE is a laborato...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkg397
更新日期：2003-06-01 00:00:00
abstract：:A crucial step in analyzing mRNA-Seq data is to accurately and efficiently map hundreds of millions of reads to the reference genome and exon junctions. Here we present OLego, an algorithm specifically designed for de novo mapping of spliced mRNA-Seq reads. OLego adopts a multiple-seed-and-extend scheme, and does not ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt216
更新日期：2013-05-01 00:00:00
abstract：:Folding of group II introns is characterized by a first slow compaction of domain 1 (D1) followed by the rapid docking of other domains to this scaffold. D1 compaction initiates in a small subregion encompassing the κ and ζ elements. These two tertiary elements are also the major interaction sites with domain 5 to for...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks1179
更新日期：2013-02-01 00:00:00
abstract：:We have identified cDNAs encoding Mel-N1, the mouse homologue of a human nervous system-specific RNA-binding protein, Hel-N1. Two major mRNA transcripts of Mel-N1 were detected predominantly in the adult mouse brain by Northern blot analysis. To gain insight into the RNA binding specificity of Mel-N1, we performed ite...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/24.11.2011
更新日期：1996-06-01 00:00:00
abstract：:The active and inactive state of transcription factors in growing cells is usually directed by allosteric physicochemical signals or metabolites, which are in turn either produced in the cell or obtained from the environment by the activity of the products of effector genes. To understand the regulatory dynamics and t...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm743
更新日期：2007-01-01 00:00:00
abstract：:Cells deficient in the Werner syndrome protein (WRN) or BRCA1 are hypersensitive to DNA interstrand cross-links (ICLs), whose repair requires nucleotide excision repair (NER) and homologous recombination (HR). However, the roles of WRN and BRCA1 in the repair of DNA ICLs are not understood and the molecular mechanisms...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkl362
更新日期：2006-05-19 00:00:00
abstract：:The interaction of the high mobility group proteins, HMG14 and HMG17, with nucleosome core particles has been studied. The results show that two molecules of HMG14/17 can be bound tightly but reversibly to each core particle and that their affinity for core particles is greater than their affinity for histone-free DNA...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/8.17.3757
更新日期：1980-09-11 00:00:00
abstract：:Findings from clinical and biological studies are often not reproducible when tested in independent cohorts. Due to the testing of a large number of hypotheses and relatively small sample sizes, results from whole-genome expression studies in particular are often not reproducible. Compared to single-study analysis, ge...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw797
更新日期：2017-01-09 00:00:00
abstract：:The Dnd (DNA degradation) phenotype, reflecting a novel DNA modification by sulfur in Streptomyces lividans 1326, was strongly aggravated when one (dndB) of the five genes (dndABCDE) controlling it was mutated. Electrophoretic banding patterns of a plasmid (pHZ209), reflecting DNA degradation, displayed a clear change...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm176
更新日期：2007-01-01 00:00:00
abstract：:This paper describes a computer program designed to look for similarities between pairs of nucleic or amino acid sequences. The program looks both for segments of perfect identity or for regions where, using a scoring matrix, a minimum value is exceeded. The results of comparisons are presented as a matrix which is di...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/10.9.2951
更新日期：1982-05-11 00:00:00
abstract：:A simplified and economical method for the attachment of 2'-deoxyribo, ribo and arabinonucleosides onto long-chain alkylamidopropanoic acid controlled-pore glass (LCAAP-CPG, P-3) is described. In this procedure, 5'-O-tritylated nucleosides are coupled directly to LCAAP-CPG in excellent yields using 1-(3-dimethylaminop...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/18.13.3813
更新日期：1990-07-11 00:00:00
abstract：:Budding yeast telomerase is mainly activated by Tel1/Mec1 (yeast ATM/ATR) on Cdc13 from late S to G2 phase of the cell cycle. Here, we demonstrated that the telomerase-recruitment domain of Cdc13 is also phosphorylated by Cdk1 at the same cell cycle stage as the Tel1/Mec1-dependent regulation. Phosphor-specific gel an...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp235
更新日期：2009-06-01 00:00:00
abstract：:The 3' splice site of the second intron (I2) of the human apolipoprotein-AII gene, (GT)16GGGCAG, is unique in that, although fully functional, a stretch of alternating guanine and thymine residues replaces the polypyrimidine tract usually associated with 3' splice junctions. The transient expression of successive 5' d...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/15.9.3787
更新日期：1987-05-11 00:00:00
abstract：:The anti-cancer drug 2',2'-difluoro-2'-deoxycytidine (dFdC) is internally incorporated into DNA in vitro. To determine the effects of this incorporation on DNA structure and function, the beta-cyanoethyl phosphoramidite of dFdC was synthesized and oligodeoxyribonucleotides containing dFdC were made using automated sol...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/20.7.1763
更新日期：1992-04-11 00:00:00
abstract：:The E. coli single-stranded binding protein (SSB) has been demonstrated in vitro to be involved in a number of replicative, DNA renaturation, and protective functions. It was shown previously that SSB can interact with exonuclease I to stimulate the hydrolysis of single-stranded DNA. We demonstrate here that E. coli S...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/22.2.247
更新日期：1994-01-25 00:00:00
abstract：:Approximately 30% of human tumors characterized to date express DNA polymerase beta (pol beta) variant proteins. Two of the polymerase beta cancer-associated variants are sequence-specific mutators, and one of them binds to DNA but has no polymerase activity. The Leu22Pro (L22P) DNA polymerase beta variant was identif...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm1053
更新日期：2008-02-01 00:00:00
abstract：:The three dimensional structures for representatives of nearly half of all protein families are now available in public databases. Thus, no matter which protein one investigates, it is increasingly likely that the 3D structure of a homolog will be known and may reveal unsuspected structure-function relationships. The ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/27.1.240
更新日期：1999-01-01 00:00:00
abstract：:We describe PReMod, a new database of genome-wide cis-regulatory module (CRM) predictions for both the human and the mouse genomes. The prediction algorithm, described previously in Blanchette et al. (2006) Genome Res., 16, 656-668, exploits the fact that many known CRMs are made of clusters of phylogenetically conser...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkl879
更新日期：2007-01-01 00:00:00
abstract：:A highly sensitive method to detect traces of aldehyde-containing apurinic/apyrimidinic (AP) sites in nucleic acids has been developed. Based on this method, a novel approach to detect DNA base mismatches recognized by the mismatch repair glycosylase MutY is demonstrated. Open chain aldehydes generated in nucleic acid...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/27.5.1316
更新日期：1999-03-01 00:00:00
