Abstract:
:The interaction of the high mobility group proteins, HMG14 and HMG17, with nucleosome core particles has been studied. The results show that two molecules of HMG14/17 can be bound tightly but reversibly to each core particle and that their affinity for core particles is greater than their affinity for histone-free DNA of core size. Thermal denaturation and nuclease digestion studies suggest that major sites of interaction are located near the ends of the nucleosome core DNA. When nucleosome preparations from chicken erythrocyte nuclei stripped of HMG proteins are partially titrated with HMG14/17, the nucleosome-HMG complex fraction is enriched in beta-globin gene sequences.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Sandeen G,Wood WI,Felsenfeld Gdoi
10.1093/nar/8.17.3757subject
Has Abstractpub_date
1980-09-11 00:00:00pages
3757-78issue
17eissn
0305-1048issn
1362-4962journal_volume
8pub_type
杂志文章abstract::Many tools are available to analyse genomes but are often challenging to use in a cell type-specific context. We have developed a method similar to the isolation of nuclei tagged in a specific cell type (INTACT) technique [Deal,R.B. and Henikoff,S. (2010) A simple method for gene expression and chromatin profiling of ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks671
更新日期:2012-10-01 00:00:00
abstract::A method is described to simultaneously amplify and sequence DNA using a new class of nucleotides containing boron. During the polymerase chain reaction, boron-modified nucleotides, i.e. 2'-deoxynucleoside 5'-alpha-[P-borano]-triphosphates, are incorporated into the product DNA. The boranophosphate linkages are resist...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/25.8.1611
更新日期:1997-04-15 00:00:00
abstract::Chicken conalbumin double-stranded cDNA (con-dscDNA) was synthesized from a laying hen oviduct mRNA preparation enriched for conalbumin mRNA (con-mRNA). The dscDNA was inserted by blunt-end ligation into the Sal I site of plasmid pBR322 which had been repaired with DNA polymerase I to create Taq I sites on each side o...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/6.7.2435
更新日期:1979-06-11 00:00:00
abstract::The Schizosaccharomyces pombe genome is one of the smallest among the free-living eukaryotes. We further reduced the S. pombe gene number by large-scale gene deletion to identify a minimal gene set required for growth under laboratory conditions. The genome-reduced strain has four deletion regions: 168.4 kb in the lef...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt233
更新日期:2013-05-01 00:00:00
abstract::Daunomycin is one of the most important agents used in anticancer chemotherapy. It interacts with DNA through intercalation of its planar chromophore between successive base pairs. The effect of intercalation on structure, dynamics and energetics is the topic of a wealth of scientific studies. In the present study, we...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkh788
更新日期:2004-09-01 00:00:00
abstract::Three different types of repetitive Eco RI fragments, which comigrate within a visible band of approximately 500 bp at gel electrophoresis of Xenopus laevis DNA Eco RI digests have been cloned and sequenced. These sequences are designated as Repetitive Eco RI Monomers: REM 1, REM 2 and REM 3. The sequences contain dir...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/12.12.4921
更新日期:1984-06-25 00:00:00
abstract::The 3' terminal nucleotide sequence of two clones containing DNA complementary to mu chain mRNA of IgM-secreting cells has been determined. The sequence shows a termination codon (UGA) adjacent to the terminal tyrosine codon for the secreted protein and a 3' non-coding region of at least 106 bases. The primary transla...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:
更新日期:1980-02-25 00:00:00
abstract::The Nucleolar Proteome Database (NOPdb) archives data on >700 proteins that were identified by multiple mass spectrometry (MS) analyses from highly purified preparations of human nucleoli, the most prominent nuclear organelle. Each protein entry is annotated with information about its corresponding gene, its domain st...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkj004
更新日期:2006-01-01 00:00:00
abstract::An improved and simplified protocol for DNA immobilization was developed to enhance DNA-DNA hybridization on microwell plates. Target DNA was immobilized by simple dry-adsorption. Efficiencies of DNA immobilization and retention were enhanced 1.4-6.5 times and 4.2-19.6 times, respectively, compared with a conventional...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/24.20.4098
更新日期:1996-10-15 00:00:00
abstract::The PR264/SC35 splicing factor belongs to the family of SR proteins which function as essential and alternative splicing factors. Here, we report that the human PR264/SC35 locus is bidirectionally transcribed. Double in situ hybridization experiments have allowed simultaneous detection of sense and antisense RNA in hu...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/25.22.4513
更新日期:1997-11-15 00:00:00
abstract::Effective drug discovery and optimization can be accelerated by techniques capable of deconvoluting the complexities often present in targeted biological systems. We report a single-molecule approach to study the binding of an alternative splicing regulator, muscleblind-like 1 protein (MBNL1), to (CUG)n = 4,6 and the ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt330
更新日期:2013-07-01 00:00:00
abstract::The interactions of self-complementary oligonucleotides with a group of metal-mediated DNA-binding drugs, including chromomycin A(3), mithramycin and the novel compound UK-1, were examined via electrospray ionization quadrupole ion trap mass spectrometry. Both chromomycin and mithramycin were shown to bind preferentia...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/29.21.e103
更新日期:2001-11-01 00:00:00
abstract::We present a simple and highly accurate computational method for operon prediction, based on intergenic distances and functional relationships between the protein products of contiguous genes, as defined by STRING database (Jensen,L.J., Kuhn,M., Stark,M., Chaffron,S., Creevey,C., Muller,J., Doerks,T., Julien,P., Roth,...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq254
更新日期:2010-07-01 00:00:00
abstract::Fourteen P-element insertion mutants at the yellow (y) gene of D. melanogaster have been analysed by restriction mapping and DNA sequencing. The distribution of the insertion sites is nonrandom, with a preferred target site in the 5'-transcribed but non-translated leader of the gene. Transcription analysis indicates t...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/16.7.3039
更新日期:1988-04-11 00:00:00
abstract::Conditional gene targeting depends on tissue and time specificity of recombination events. Endogenous promoters are often used to drive various transgenic constructs. To avoid the problems associated with reconstituting a specific expression pattern in transgenic animals by this method, we tested the internal ribosome...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/27.6.1552
更新日期:1999-03-15 00:00:00
abstract::The Protein Data Bank in Europe (PDBe, pdbe.org) is actively engaged in the deposition, annotation, remediation, enrichment and dissemination of macromolecular structure data. This paper describes new developments and improvements at PDBe addressing three challenging areas: data enrichment, data dissemination and func...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx1070
更新日期:2018-01-04 00:00:00
abstract::Contrary to the expectation, the Merrifield polystyrene resin, 2% cross-linked by divinylbenzene, is as efficient as the polyacrylmorpholide resin for the synthesis of polydeoxyribonucleotides using a phosphotriester method. On the Merrifield resin, the tetradecamer, dTpCpGpTpCpApApCpTpGpGpCpTpT, and the hexadecamer, ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/8.22.5507
更新日期:1980-11-25 00:00:00
abstract::Nucleotide sequences of large T1 ribonuclease fragments of 18S ribosomal RNA of Novikoff rat ascites hepatoma cells and chicken lymphoblastoid cells were determined and compared. Among the 19 large T1 ribonuclease fragments examined of rat 18S ribosomal RNA, 12 fragments were found to be the same in chicken 18S riboso...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/7.5.1131
更新日期:1979-11-10 00:00:00
abstract::In two-colour microarrays, the ratio of signal intensities of two co-hybridized samples is used as a relative measure of gene expression. Ratio-based analysis becomes complicated and inefficient in multi-class comparisons. We therefore investigated the validity of an intensity-based analysis procedure. To this end, tw...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gnh038
更新日期:2004-02-24 00:00:00
abstract::DNA polymerase (Pol) beta null mouse embryonic fibroblasts provide a useful cell system to investigate the effects of alterations in base excision repair (BER) on genome stability. These cells are characterized by hypersensitivity to the cytotoxic effects of methyl methanesulfonate (MMS) and by decreased repair of the...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gki168
更新日期:2005-01-12 00:00:00
abstract::The Histone Database (HDB) is an annotated and searchable collection of all full-length sequences and structures of histone and non-histone proteins containing the histone fold motif. These sequences are both eukaryotic and archaeal in origin. Several new histone fold-containing proteins have been identified, includin...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/28.1.320
更新日期:2000-01-01 00:00:00
abstract::Transcription start and termination sites were mapped in the E. coli replication origin, oriC. Outward transcription from within oriC (promoters Pori-r and Pori-l) was found to start in vivo at position 178 for Pori-l and at positions 294 and 304 for Pori-r, respectively. These transcripts were terminated after 100-15...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/15.6.2479
更新日期:1987-03-25 00:00:00
abstract::In this paper, we report investigations, based on circular dichroism, nuclear magnetic resonance spectroscopy and electrophoresis methods, on three oligonucleotide sequences, each containing one 3'-3' and two 5'-5' inversion of polarity sites, and four G-runs with a variable number of residues, namely two, three and f...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx566
更新日期:2017-08-21 00:00:00
abstract::Uracil-Specific Exision Reagent (USER) fusion is a recently developed technique that allows for assembly of multiple DNA fragments in a few simple steps. However, designing primers for USER fusion is both tedious and time consuming. Here, we present the Primer Help for USER (PHUSER) software, a novel tool for designin...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr394
更新日期:2011-07-01 00:00:00
abstract::Previous evidence indicates that telomeres resemble common fragile sites and present a challenge for DNA replication. The precise impediments to replication fork progression at telomeric TTAGGG repeats are unknown, but are proposed to include G-quadruplexes (G4) on the G-rich strand. Here we examined DNA synthesis and...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt813
更新日期:2013-12-01 00:00:00
abstract::The active form of Escherichia coli DNA polymerase V responsible for damage-induced mutagenesis is a multiprotein complex (UmuD'(2)C-RecA-ATP), called pol V Mut. Optimal activity of pol V Mut in vitro is observed on an SSB-coated single-stranded circular DNA template in the presence of the β/γ complex and a transactiv...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks233
更新日期:2012-07-01 00:00:00
abstract::We have studied the effect of temperature on transcription initiation in vitro at two related promoters ga/Pcon and ga/P1, which have the same nucleotide sequence around the -10 region and transcription start site, but differ in upstream sequences. One of the promoters, ga/Pcon, carries the consensus -35 hexamer, 5'TT...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/19.22.6113
更新日期:1991-11-25 00:00:00
abstract::We have measured the amount of 5S-ribosomal DNA in the genomes of Xenopus laevis, Triturus cristatus carnifex and Ambystoma mexicanum, three species of Amphibians which have widely different C-values. Our best estimate is that these organisms have about 24,000, 32,000 and 61,000 5S-genes per haploid genome respectivel...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/11.8.2381
更新日期:1983-04-25 00:00:00
abstract::Non-protein-coding RNAs (ncRNAs) fulfill a wide range of cellular functions from protein synthesis to regulation of gene expression. Identification of novel regulatory ncRNAs by experimental approaches commonly includes the generation of specialized cDNA libraries encoding small ncRNA species. However, such identifica...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm244
更新日期:2007-01-01 00:00:00
abstract::The mouse c-Ki-ras protooncogene promoter contains a homopurine-homopyrimidine domain that exhibits S1 nuclease sensitivity in vitro. We have studied the structure of this DNA region in a supercoiled state using a number of chemical probes for non-B DNA conformations including diethyl pyrocarbonate, osmium tetroxide, ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/19.23.6527
更新日期:1991-12-11 00:00:00