The accumulation of MMS-induced single strand breaks in G1 phase is recombinogenic in DNA polymerase beta defective mammalian cells.

abstract：:We have assessed the ability of nucleosomes to influence the formation of mammalian topoisomerase II-DNA complexes by mapping the sites of cleavage induced by four unrelated topoisomerase II inhibitors in naked versus nucleosome-reconstituted SV40 DNA. DNA fragments were reconstituted with histone octamers from HeLa c...

journal_title：Nucleic acids research

authors： Capranico G,Jaxel C,Roberge M,Kohn KW,Pommier Y

更新日期：1990-08-11 00:00:00

abstract：:The inducibility of the vir genes (virA, -B, -C, -D, -E, and -G) on pRiA4 was examined at the transcriptional level, and the RNA-starting sites were determined by S1-nuclease mapping and primer-extension experiments. All of these genes were inducible, while virA, -E, and -G were transcribed even under noninducing cond...

journal_title：Nucleic acids research

authors： Aoyama T,Takanami M,Oka A

更新日期：1989-11-11 00:00:00

abstract：:The discontinuous synthesis of the complementary strands of polyoma DNA in isolated nuclei has been studied by hybridization techniques. The relative amounts of the newly synthesized complementary strands were compared by separately annealing them to denatured HpaII restriction fragments. In every case an excess (1.4-...

journal_title：Nucleic acids research

authors： Flory PJ Jr

更新日期：1977-01-01 00:00:00

abstract：:The primary rRNA transcript contains a large external transcribed spacer (ETS) approximately 4,000 nucleotides in length. We have used subcloned DNA probes derived from the 5' end of the ETS in conjunction with Northern blot analysis of murine nuclear RNA to examine processing of this region. In agreement with the res...

journal_title：Nucleic acids research

authors： Fetherston J,Werner E,Patterson R

更新日期：1984-09-25 00:00:00

abstract：:Bacillus stearothermophilus large ribosomal subunits were reconstituted in the presence of 5S rRNAs from different origins and tested for their biological activities. The results obtained have shown that eubacterial and archaebacterial 5S rRNAs can easily substitute for B. stearothermophilus 5S rRNA in the reconstitut...

journal_title：Nucleic acids research

authors： Hartmann RK,Vogel DW,Walker RT,Erdmann VA

更新日期：1988-04-25 00:00:00

abstract：:Mycobacterium tuberculosis (Mtb) Cmr (Rv1675c) is a CRP/FNR family transcription factor known to be responsive to cAMP levels and during macrophage infections. However, Cmr's DNA binding properties, cellular targets and overall role in tuberculosis (TB) complex bacteria have not been characterized. In this study, we u...

journal_title：Nucleic acids research

authors： Ranganathan S,Bai G,Lyubetskaya A,Knapp GS,Peterson MW,Gazdik M,C Gomes AL,Galagan JE,McDonough KA

更新日期：2016-01-08 00:00:00

abstract：:Nuclear organization of chromatin is an important level of genome regulation with positional changes of genes occurring during reprogramming. Inherent variability of biological specimens, wide variety of sample preparation and imaging conditions, though pose significant challenges to data analysis and comparison. Here...

journal_title：Nucleic acids research

authors： Jost KL,Haase S,Smeets D,Schrode N,Schmiedel JM,Bertulat B,Herzel H,Cremer M,Cardoso MC

更新日期：2011-09-01 00:00:00

abstract：:We have examined the replication patterns of ribosomal DNA plasmids in vivo and in vitro using Xenopus eggs. Plasmids carrying different parts of the Xenopus ribosomal DNA sequence were allowed to replicate either in vitro in an egg extract or in vivo after microinjection into unfertilized eggs. The replication interm...

journal_title：Nucleic acids research

authors： Hyrien O,Méchali M

更新日期：1992-04-11 00:00:00

abstract：:MicroRNAs (miRNA) are short single-stranded RNA molecules derived from hairpin-forming precursors that play a crucial role as post-transcriptional regulators in eukaryotes and viruses. In the past years, many microRNA target genes (MTGs) have been identified experimentally. However, because of the high costs of experi...

journal_title：Nucleic acids research

authors： Leclercq M,Diallo AB,Blanchette M

更新日期：2017-01-25 00:00:00

abstract：:We report here the first successful generation of a chromosome-enriched library from flow sorted plant chromosomes. Chromosomes with a characteristic DNA content (a peak) were sorted from a synchronized cell culture (TaKB1, derived from Triticum aestivum). A HpaII library was constructed from the sorted chromosomes an...

journal_title：Nucleic acids research

authors： Wang ML,Leitch AR,Schwarzacher T,Heslop-Harrison JS,Moore G

更新日期：1992-04-25 00:00:00

abstract：:The molecular basis for the DNA binding specificity of the thyroid transcription factor 1 homeodomain (TTF-1HD) has been investigated. Methylation and ethylation interference experiments show that the TTF-1HD alone recapitulates the DNA binding properties of the entire protein. Studies carried out with mutant derivati...

journal_title：Nucleic acids research

authors： Damante G,Fabbro D,Pellizzari L,Civitareale D,Guazzi S,Polycarpou-Schwartz M,Cauci S,Quadrifoglio F,Formisano S,Di Lauro R

更新日期：1994-08-11 00:00:00

abstract：:In the haploid genome of Xenopus laevis there are two genes coding for the r-protein L14. It is not known if they are located on the same chromosome. cDNA clones deriving from the transcripts of the two genes have been isolated from an oocyte messenger cDNA bank showing that they are both expressed. We have studied th...

journal_title：Nucleic acids research

authors： Beccari E,Mazzetti P,Mileo A,Bozzoni I,Pierandrei-Amaldi P,Amaldi F

更新日期：1986-10-10 00:00:00

abstract：:Replication Protein A (RPA) is a critical complex that acts in replication and promotes homologous recombination by allowing recombinase recruitment to processed DSB ends. Most organisms possess three RPA subunits (RPA1, RPA2, RPA3) that form a trimeric complex critical for viability. The Caenorhabditis elegans genome...

journal_title：Nucleic acids research

authors： Hefel A,Honda M,Cronin N,Harrell K,Patel P,Spies M,Smolikove S

更新日期：2021-01-21 00:00:00

abstract：:Heptadecaoligodeoxyribonucleotides containing one or more of the bases, 6H,8H-3,4-dihydropyrimido[4,5-c][1,2]oxazin-7-one (P), 2-amino-6-methoxyaminopurine (K), and hypoxanthine (I) and combinations of P with K and I have been synthesised on a DNA synthesiser. The stability of duplexes containing these basemodified ol...

journal_title：Nucleic acids research

authors： Lin PK,Brown DM

更新日期：1992-10-11 00:00:00

abstract：:Although secondary structure predictions of an individual RNA sequence have been widely used in a number of sequence analyses of RNAs, accuracy is still limited. Recently, we proposed a method (called 'CentroidHomfold'), which includes information about homologous sequences into the prediction of the secondary structu...

journal_title：Nucleic acids research

authors： Hamada M,Yamada K,Sato K,Frith MC,Asai K

更新日期：2011-07-01 00:00:00

abstract：:E2A is a member of the E-protein family of transcription factors. Previous studies have reported context-dependent regulation of E2A-dependent transcription. For example, whereas the E2A portion of the E2A-Pbx1 leukemia fusion protein mediates robust transcriptional activation in t(1;19) acute lymphoblastic leukemia, ...

journal_title：Nucleic acids research

authors： Gow CH,Guo C,Wang D,Hu Q,Zhang J

更新日期：2014-01-01 00:00:00

abstract：:For the isolation of single stranded plasmid DNA, various E. coli and E. coli-Streptomyces shuttle plasmids were equipped with the phage f1 replication origin. The transformation of some representative Streptomyces species with plasmid vectors occurred irrespective of whether single or double stranded DNA was used. In...

journal_title：Nucleic acids research

authors： Hillemann D,Pühler A,Wohlleben W

更新日期：1991-02-25 00:00:00

abstract：:Human transcription factor Sp1 contains three contiguous repeats of the C2H2-type zinc finger motif and binds to the decanucleotide sequence 5'-(G/T)GGGCGG(G/A)(G/A)(C/T)-3' (GC box). In order to determine whether the three-zinc finger peptide Sp1(530-623) has selectivity for sequence outside the GC box, we used a sel...

journal_title：Nucleic acids research

authors： Nagaoka M,Shiraishi Y,Sugiura Y

更新日期：2001-12-15 00:00:00

abstract：:We have examined the formation of alternate strand triple-helices at the target sequence A11(TC)6.(GA)6T11 using the oligonucleotides T11(AG)6 and T11(TG)6, by DNase I footprinting. These third strands were designed so as to form parallel T.AT triplets together with antiparallel G.GC and A.AT or T.AT triplets. We find...

journal_title：Nucleic acids research

authors： Washbrook E,Fox KR

更新日期：1994-09-25 00:00:00

abstract：:LINKER was developed as an online server to assist biomedical researchers to design linker sequences for constructing functional fusion proteins. The program automatically generates a set of peptide sequences that are known to adopt extended conformations as determined by X-ray crystallography and NMR. In addition to ...

journal_title：Nucleic acids research

authors： Xue F,Gu Z,Feng JA

更新日期：2004-07-01 00:00:00

abstract：:Post-transcriptional gene regulation is mediated through complex networks of protein-RNA interactions. The targets of only a few RNA binding proteins (RBPs) are known, even in the well-characterized budding yeast. In silico prediction of protein-RNA interactions is therefore useful to guide experiments and to provide ...

journal_title：Nucleic acids research

authors： Pancaldi V,Bähler J

更新日期：2011-08-01 00:00:00

abstract：:A linear method for the search of eukaryotic nuclear tRNA genes in DNA databases is described. Based on a modified version of the general weight matrix procedure, our algorithm relies on the recognition of two intragenic control regions known as A and B boxes, a transcription termination signal, and on the evaluation ...

journal_title：Nucleic acids research

authors： Pavesi A,Conterio F,Bolchi A,Dieci G,Ottonello S

更新日期：1994-04-11 00:00:00

abstract：:Module identification is a frequently used approach for mining local structures with more significance in global networks. Recently, a wide variety of bilayer networks are emerging to characterize the more complex biological processes. In the light of special topological properties of bilayer networks and the accompan...

journal_title：Nucleic acids research

authors： Xu Y,Guo M,Liu X,Wang C,Liu Y,Liu G

更新日期：2016-11-16 00:00:00

abstract：:The chemical shifts of the well-resolved ring nitrogen protons of uracil and a series of substituted uracils, including pseudouridine (5-ribosyl-uracil) and uridine (1-ribosyl-uracil) were determined using nuclear magnetic resonance (NMR). The observed chemical shifts suggest the existence of an atypical syn conformat...

journal_title：Nucleic acids research

authors： Hurd RE,Reid BR

更新日期：1977-08-01 00:00:00

abstract：:A newly discovered group of spherical plant viruses contains a bipartite genome consisting of a single-strand linear RNA molecule (RNA 1, Mr 1.5 x 10(6) ), and a single-strand, covalently closed circular viroid-like RNA molecule (RNA 2, Mr approximately 125,000). The nucleotide sequences of the RNA 2 of two of these, ...

journal_title：Nucleic acids research

authors： Haseloff J,Symons RH

更新日期：1982-06-25 00:00:00

abstract：:The exact sites at which a number of drugs inhibit the nick translation of DNA by E.coli DNA polymerase-I have been pinpointed. In order to do this, a method has been developed for sequencing double-stranded plasmid DNA from the site of a specifically induced nick. The initial experiments have concentrated on analysis...

journal_title：Nucleic acids research

authors： Wilkins RJ

更新日期：1982-11-25 00:00:00

abstract：:The poly(A) signal and downstream elements with transcriptional pausing activity play an important role in termination of RNA polymerase II transcription. We show that an intronic sequence derived from the plant seed protein gene (AmA1) specifically acts as a transcriptional terminator in the fission yeast, Schizosacc...

journal_title：Nucleic acids research

authors： Chakraborty S,Sarmah B,Chakraborty N,Datta A

更新日期：2002-07-01 00:00:00

abstract：:Transcription factor binding sites (TFBSs) are short DNA sequences interacting with transcription factors (TFs), which regulate gene expression. Due to the relatively short length of such binding sites, it is largely unclear how the specificity of protein-DNA interaction is achieved. Here, we have performed a genome-w...

journal_title：Nucleic acids research

authors： Zhang C,Xuan Z,Otto S,Hover JR,McCorkle SR,Mandel G,Zhang MQ

更新日期：2006-05-02 00:00:00

abstract：:We present a www server for AUGUSTUS, a novel software program for ab initio gene prediction in eukaryotic genomic sequences. Our method is based on a generalized Hidden Markov Model with a new method for modeling the intron length distribution. This method allows approximation of the true intron length distribution m...

journal_title：Nucleic acids research

authors： Stanke M,Steinkamp R,Waack S,Morgenstern B

更新日期：2004-07-01 00:00:00

abstract：:Plasmids containing a dihydrofolate reductase (DHFR) expression unit were transfected into DHFR-deficient Chinese hamster ovary (CHO) cells. Methotrexate exposure was used to select cells with amplified DHFR sequences. Three cell lines were isolated containing amplified copies of transfected DNA that had integrated in...

journal_title：Nucleic acids research

authors： Heartlein MW,Latt SA

更新日期：1989-02-25 00:00:00