Use of an internal ribosome entry site for bicistronic expression of Cre recombinase or rtTA transactivator.

Abstract:

:Conditional gene targeting depends on tissue and time specificity of recombination events. Endogenous promoters are often used to drive various transgenic constructs. To avoid the problems associated with reconstituting a specific expression pattern in transgenic animals by this method, we tested the internal ribosome entry site of the encephalomyocarditis virus, to enable linkage of the Cre recombinase or rtTA trans-activator to 3' untranslated ends of endogenous genes. Here we report that these constructs function effectively in COS cells. The data suggest that these cassettes will be appropriate for 3' targeting of mouse genes.

journal_name

Nucleic Acids Res

journal_title

Nucleic acids research

authors

Lindeberg J,Ebendal T

doi

10.1093/nar/27.6.1552

keywords:

subject

Has Abstract

pub_date

1999-03-15 00:00:00

pages

1552-4

issue

6

eissn

0305-1048

issn

1362-4962

pii

gkc261

journal_volume

27

pub_type

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