Abstract:
:Contrary to the expectation, the Merrifield polystyrene resin, 2% cross-linked by divinylbenzene, is as efficient as the polyacrylmorpholide resin for the synthesis of polydeoxyribonucleotides using a phosphotriester method. On the Merrifield resin, the tetradecamer, dTpCpGpTpCpApApCpTpGpGpCpTpT, and the hexadecamer, dCpCpApGpTpCpApCpGpApCpGpTpTpGpT, were synthesized by the phosphotriester method using di and trinucleotide blocks as coupling units.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Miyoshi K,Arentzen R,Huang T,Itakura Kdoi
10.1093/nar/8.22.5507subject
Has Abstractpub_date
1980-11-25 00:00:00pages
5507-17issue
22eissn
0305-1048issn
1362-4962journal_volume
8pub_type
杂志文章abstract::DNA curvature plays a well-characterized role in many transcriptional regulation mechanisms. We present evidence for the conservation of curvature signals in putative regulatory regions of several archaeal and eubacterial genomes. Genes with highly curved upstream regions were identified in orthologous groups, based o...
journal_title:Nucleic acids research
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abstract::1731, a Drosophila retrotransposon was first described as having a transcription activity which was negatively regulated by 20-hydroxyecdysone (20-OH), the steroid molting hormone of insects. Using constructions expressing the bacterial chloramphenicol-acetyltransferase (CAT) gene under the control of the entire or de...
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journal_title:Nucleic acids research
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更新日期:2001-10-15 00:00:00
abstract::The thermodynamics of 13 hybridization reactions between 10 base DNA sequences of design 5'-ATGCXYATGC-3' with X, Y = A, C, G, T and their complementary PNA and DNA sequences were determined from isothermal titration calorimetry (ITC) measurements at ambient temperature. For the PNA/DNA hybridization reactions, the bi...
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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更新日期:2004-05-11 00:00:00
abstract::DNA synthesized by in vitro transcription on rat interferon (IFN) mRNA has been cloned and amplified as recombinant DNA. The nucleotide sequence of these rat IFN cDNA clones revealed i. the partial presence of the coding region of the gene and ii all cDNA clones were derived from the same subtype of rat IFN-alpha mRNA...
journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2016-02-18 00:00:00
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journal_title:Nucleic acids research
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更新日期:1983-03-11 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1982-12-11 00:00:00
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更新日期:2004-02-20 00:00:00
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更新日期:2016-05-19 00:00:00
abstract::The C (pifC) protein of miniF represses transcription of its own gene by binding to the pif operator (pifO); it is also needed for replication initiated from the miniF primary origin (ori-1). We have determined the nucleotide sequence of the C gene. The gene has been inserted into an expression vector under Ptrp contr...
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更新日期:2011-04-01 00:00:00
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更新日期:1978-10-01 00:00:00