The miniF plasmid C protein: sequence, purification and DNA binding.

Abstract:

:The C (pifC) protein of miniF represses transcription of its own gene by binding to the pif operator (pifO); it is also needed for replication initiated from the miniF primary origin (ori-1). We have determined the nucleotide sequence of the C gene. The gene has been inserted into an expression vector under Ptrp control where it is expressed at high levels. The C protein has been purified from cells carrying the Ptrp-C plasmid, and a preliminary study of C protein-DNA binding properties has been carried out. C protein binds strongly to pifO, and weakly to sequences in the ori-1 region.

journal_name

Nucleic Acids Res

journal_title

Nucleic acids research

authors

Caughey PA,de Feyter R,Lane HE

doi

10.1093/nar/14.24.9699

subject

Has Abstract

pub_date

1986-12-22 00:00:00

pages

9699-712

issue

24

eissn

0305-1048

issn

1362-4962

journal_volume

14

pub_type

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