Abstract:
:The C (pifC) protein of miniF represses transcription of its own gene by binding to the pif operator (pifO); it is also needed for replication initiated from the miniF primary origin (ori-1). We have determined the nucleotide sequence of the C gene. The gene has been inserted into an expression vector under Ptrp control where it is expressed at high levels. The C protein has been purified from cells carrying the Ptrp-C plasmid, and a preliminary study of C protein-DNA binding properties has been carried out. C protein binds strongly to pifO, and weakly to sequences in the ori-1 region.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Caughey PA,de Feyter R,Lane HEdoi
10.1093/nar/14.24.9699subject
Has Abstractpub_date
1986-12-22 00:00:00pages
9699-712issue
24eissn
0305-1048issn
1362-4962journal_volume
14pub_type
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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更新日期:1994-03-25 00:00:00
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journal_title:Nucleic acids research
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更新日期:2006-01-01 00:00:00
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journal_title:Nucleic acids research
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更新日期:2005-03-23 00:00:00
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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更新日期:1987-09-25 00:00:00
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更新日期:2017-01-25 00:00:00
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journal_title:Nucleic acids research
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更新日期:2011-07-01 00:00:00
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journal_title:Nucleic acids research
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更新日期:2012-03-01 00:00:00