Abstract:
:The C (pifC) protein of miniF represses transcription of its own gene by binding to the pif operator (pifO); it is also needed for replication initiated from the miniF primary origin (ori-1). We have determined the nucleotide sequence of the C gene. The gene has been inserted into an expression vector under Ptrp control where it is expressed at high levels. The C protein has been purified from cells carrying the Ptrp-C plasmid, and a preliminary study of C protein-DNA binding properties has been carried out. C protein binds strongly to pifO, and weakly to sequences in the ori-1 region.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Caughey PA,de Feyter R,Lane HEdoi
10.1093/nar/14.24.9699subject
Has Abstractpub_date
1986-12-22 00:00:00pages
9699-712issue
24eissn
0305-1048issn
1362-4962journal_volume
14pub_type
杂志文章abstract::An adequate supply of nucleotides is essential for DNA replication and DNA repair. Moreover, inhibition of TTP synthesis can cause cell death by a poorly characterized mechanism called thymine-less death. In the yeast Saccharomyces cerevisiae, the genes encoding thymidylate synthetase (CDC21) and thymidylate kinase (C...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gki219
更新日期:2005-01-28 00:00:00
abstract::The role of TATA elements in the expression of a mammalian promoter was investigated in the fission yeast Schizosaccharomyces pombe, by studying the human adenovirus E2-early promoter. This is a unique dual promoter with two nonconsensus TATA elements directing transcription from two cap sites, +1 and -26. A sequence ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/21.11.2737
更新日期:1993-06-11 00:00:00
abstract::Arginyl-tRNA synthetase (ArgRS) catalyses AMP- and PPi-independent deacylation of Arg-tRNAArg in the presence of cysteine. A dipeptide, Arg-Cys, is a product of this deacylation reaction. Similar reaction with homocysteine yields Arg-Hcy. Arginine is a noncompetitive inhibitor of the cysteine-dependent deacylation whi...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/23.22.4608
更新日期:1995-11-25 00:00:00
abstract::Activation of the transcription factor NF-kappaB is critical for the tumor necrosis factor-alpha (TNF-alpha)-induced inflammatory response. Here we report the complete gene expression profile from activated microvascular endothelial cells emphasizing the direct contribution of the NF-kappaB pathway. Human microvascula...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gki836
更新日期:2005-09-21 00:00:00
abstract::Gene promoters are enriched in guanine clusters that potentially fold into quadruplex structures. Such quadruplexes were implicated in the regulation of gene expression, plausibly by interacting with transcription factors. We showed previously that homodimers of the myogenic transcription factor MyoD bound in vitro mo...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp1208
更新日期:2010-04-01 00:00:00
abstract::For efficient DNA cleavage, the Type III restriction endonuclease EcoP15I communicates with two inversely oriented recognition sites in an ATP-dependent process. EcoP15I consists of methylation (Mod) and restriction (Res) subunits forming a multifunctional enzyme complex able to methylate or to cleave DNA. In this stu...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr1239
更新日期:2012-04-01 00:00:00
abstract::DNA transposon-based vectors have emerged as gene vehicles with a wide biomedical and therapeutic potential. So far, genomic insertion of such vectors has relied on the co-delivery of genetic material encoding the gene-inserting transposase protein, raising concerns related to persistent expression, insertional mutage...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt1163
更新日期:2014-02-01 00:00:00
abstract::We have developed general methods for joining together, via cleavable disulfide bonds, either two unprotected polynucleotides or a polynucleotide and a peptide or protein. To join two oligonucleotides, each is first converted to an adduct in which cystamine is joined to the 5'-terminal phosphate of the oligonucleotide...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/16.9.3671
更新日期:1988-05-11 00:00:00
abstract::Circular dichroism in the 300-360 nm region and fluorescence induced by intercaltating binding of ethidum bromide to both DNA and RNA components were studied in isolated HeLa nucleoli. Both DNA and RNA compoents contribute to the induced dichroic elliticity. Digestion of nucleoli by RNase or DNase shows that most of t...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/3.8.1857
更新日期:1976-08-01 00:00:00
abstract::In the brome mosaic virus (BMV) virion, the coat protein (CP) selectively contacts the RNA motifs that regulate translation and RNA replication (Hoover et al., 2016. J. Virol. 90, 7748). We hypothesize that the unstructured N-terminal arm (NTA) of the BMV CP can specifically recognize RNA motifs. Using ion mobility sp...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx1087
更新日期:2018-01-09 00:00:00
abstract::Corynebacterium diphtheriae is a Gram-positive, non-spore forming, non-motile, pleomorphic rod belonging to the genus Corynebacterium and the actinomycete group of organisms. The organism produces a potent bacteriophage-encoded protein exotoxin, diphtheria toxin (DT), which causes the symptoms of diphtheria. This pote...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg874
更新日期:2003-11-15 00:00:00
abstract::Ribosome recycling factor (RRF), elongation factor G (EF-G) and GTP split 70S ribosomes into subunits. Here, we demonstrated that the splitting was transient and the exhaustion of GTP resulted in re-association of the split subunits into 70S ribosomes unless IF3 (initiation factor 3) was present. However, the splittin...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkn647
更新日期:2008-12-01 00:00:00
abstract::Chicken-erythrocyte inner histone tetramer has been complexed with several natural and synthetic DNA duplexes by salt-gradient dialysis at various protein/DNA ratios. The resulting complexes, in low-ionic-strength buffer, have been examined by electron microscopy, circular dichroism, and thermal denaturation. Electron...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/5.10.3603
更新日期:1978-10-01 00:00:00
abstract::Explicit solvent molecular dynamics (MD) was used to describe the intrinsic flexibility of the helix 42-44 portion of the 23S rRNA (abbreviated as Kt-42+rGAC; kink-turn 42 and GTPase-associated center rRNA). The bottom part of this molecule consists of alternating rigid and flexible segments. The first flexible segmen...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm245
更新日期:2007-01-01 00:00:00
abstract::The small subunit (SSU) processome is a large ribonucleoprotein that is required for maturation of the 18S rRNA of the ribosome. Recently, a missense mutation in the C-terminus of an SSU processome protein, Utp4/Cirhin, was reported to cause North American Indian childhood cirrhosis (NAIC). In this study, we use Sacch...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq185
更新日期:2010-08-01 00:00:00
abstract::GenBank (R) is a comprehensive database that contains publicly available nucleotide sequences for more than 260 000 named organisms, obtained primarily through submissions from individual laboratories and batch submissions from large-scale sequencing projects. Most submissions are made using the web-based BankIt or st...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm929
更新日期:2008-01-01 00:00:00
abstract::With the recent increase in the number of mammalian genomes being sequenced, large-scale genome scans for human-specific positive selection are now possible. Selection can be inferred through phylogenetic analysis by comparing the rates of silent and replacement substitution between related species. Maximum-likelihood...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm764
更新日期:2008-01-01 00:00:00
abstract::KRAS is one of the most mutated genes in human cancer. It is controlled by a G4 motif located upstream of the transcription start site. In this paper, we demonstrate that 8-oxoguanine (8-oxoG), being more abundant in G4 than in non-G4 regions, is a new player in the regulation of this oncogene. We designed oligonucleo...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx1142
更新日期:2018-01-25 00:00:00
abstract::In plants, nuclear multisubunit RNA polymerases IV and V are RNA Polymerase II-related enzymes that synthesize non-coding RNAs for RNA-directed DNA methylation (RdDM) and transcriptional gene silencing. Here, we tested the importance of the C-terminal domain (CTD) of Pol IV's largest subunit given that the Pol II CTD ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkz615
更新日期:2019-09-26 00:00:00
abstract::Three novel DNA-binding proteins with apparent molecular masses of 7, 10 and 30 kDa have been isolated from the hyperthermophilic methanogen Methanopyrus kandleri. The proteins were identified using a blot overlay assay that was modified to emulate the high ionic strength intracellular environment of M.kandleri protei...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/30.3.685
更新日期:2002-02-01 00:00:00
abstract::The reaction of fluorescamine with primary amino groups of tRNAs was investigated. The reagent was attached under mild conditions to the 3'-end of tRNAPhe-C-C-A(3'NH) from yeast and to the minor nucleoside x in E. coli tRNAArg, tRNALys, tRNAMet, tRNAIle and tRNAPhe. The primary aliphatic amino groups of these tRNAs re...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/5.12.4837
更新日期:1978-12-01 00:00:00
abstract::Ribosomal protein (rp)S5 belongs to the family of the highly conserved rp's that contains rpS7 from prokaryotes and rpS5 from eukaryotes. Alignment of rpS5/rpS7 from metazoans (Homo sapiens), fungi (Saccharomyces cerevisiae) and bacteria (Escherichia coli) shows that the proteins contain a conserved central/C-terminal...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp1113
更新日期:2010-03-01 00:00:00
abstract::Rapid and transient activation of both c-fos transcription and mRNA accumulation occurs when resting CCL39 hamster fibroblasts are serum-stimulated to grow. By using several combinations of serum and cycloheximide, a protein synthesis inhibitor, we showed that: i) addition of cycloheximide to resting cell elicits an i...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/15.14.5657
更新日期:1987-07-24 00:00:00
abstract::A dot hybridization method is presented for rapidly determining the relative concentrations of nucleic acids in a mixture, as well as the extent of sequence homology between related RNA or DNA species. ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/7.6.1541
更新日期:1979-11-24 00:00:00
abstract::Potential pathways that could account for observed rapid rates of transcription reinitiation were explored. A nuclear extract system was established in which reinitiation rates were observed to be kinetically facilitated and in which the rate was sensitive to TATA box mutation. Kinetic facilitation of functional compl...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/27.3.831
更新日期:1999-02-01 00:00:00
abstract::Leishmania species are protozoan parasites whose remarkably plastic genome limits the establishment of effective genetic manipulation and leishmaniasis treatment. The strategies used by Leishmania to maintain its genome while allowing variability are not fully understood. Here, we used DiCre-mediated conditional gene ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky1017
更新日期:2018-12-14 00:00:00
abstract::TOP mRNAs are vertebrate transcripts which contain a 5'terminal oligopyrimidine tract (5'TOP), encode for ribosomal proteins and elongation factors 1alpha and 2, and are candidates for growth-dependent translational control mediated through their 5'TOP. In the present study we show that elongation factor 2 (EF2) mRNA ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/25.5.995
更新日期:1997-03-01 00:00:00
abstract::We present a tool for the prediction of mRNA 3'-processing (cleavage and polyadenylation) sites in the yeast Saccharomyces cerevisiae, based on a discrete state-space model or hidden Markov model. Comparison of predicted sites with experimentally verified 3'-processing sites indicates good agreement. All predicted or ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/30.8.1851
更新日期:2002-04-15 00:00:00
abstract::This review presents detailed information about the structure of triplet repeat RNA and addresses the simple sequence repeats of normal and expanded lengths in the context of the physiological and pathogenic roles played in human cells. First, we discuss the occurrence and frequency of various trinucleotide repeats in...
journal_title:Nucleic acids research
pub_type: 杂志文章,评审
doi:10.1093/nar/gkr729
更新日期:2012-01-01 00:00:00
abstract::We have determined the nucleotide sequences of long terminal repeat (LTR) regions of Syrian hamster intracisternal A particle (IAP) genes. The size of the LTRs was 350 base-pairs (bp) and 376 bp in two clones, H10 and H18, respectively. Two LTRs at both ends of the IAP gene were linked to directly repeating 6 bp hamst...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/11.20.7169
更新日期:1983-10-25 00:00:00