Abstract:
:The C (pifC) protein of miniF represses transcription of its own gene by binding to the pif operator (pifO); it is also needed for replication initiated from the miniF primary origin (ori-1). We have determined the nucleotide sequence of the C gene. The gene has been inserted into an expression vector under Ptrp control where it is expressed at high levels. The C protein has been purified from cells carrying the Ptrp-C plasmid, and a preliminary study of C protein-DNA binding properties has been carried out. C protein binds strongly to pifO, and weakly to sequences in the ori-1 region.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Caughey PA,de Feyter R,Lane HEdoi
10.1093/nar/14.24.9699subject
Has Abstractpub_date
1986-12-22 00:00:00pages
9699-712issue
24eissn
0305-1048issn
1362-4962journal_volume
14pub_type
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更新日期:2006-07-26 00:00:00
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更新日期:2008-04-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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abstract::In plants, small interfering RNAs (siRNAs) can trigger a silencing signal that may spread within a tissue to adjacent cells or even systemically to other organs. Movement of the signal is initially limited to a few cells, but in some cases the signal can be amplified and travel over larger distances. How far silencing...
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abstract::The sarcomeric myosin gene, Myh7b, encodes an intronic microRNA, miR-499, which regulates cardiac and skeletal muscle biology, yet little is known about its transcriptional regulation. To identify the transcription factors involved in regulating Myh7b/miR-499 gene expression, we have mapped the transcriptional start s...
journal_title:Nucleic acids research
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更新日期:2012-08-01 00:00:00
abstract::Genes from yeast to mammals are frequently subject to non-coding transcription of their antisense strand; however the genome-wide role for antisense transcription remains elusive. As transcription influences chromatin structure, we took a genome-wide approach to assess which chromatin features are associated with nasc...
journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2015-09-18 00:00:00
abstract::Cyclic AMP response element binding protein (CREB) activates transcription of cAMP response element (CRE)-containing promoters following an elevation of intracellular cAMP. Here we show that CREB and the highly related protein ATF-1 are also potent transcription inhibitors. Strikingly, CREB inhibits transcription of m...
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doi:10.1093/nar/21.12.2907
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abstract::We describe the first polyA-containing processed pseudogene reported in the chicken. It includes a 0.52 kb open reading frame which could encode a 175 amino acid protein. The putative protein shows extensive homology to the ras oncogene superfamily, being most closely related to the yeast protein YP2. It is one of the...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/14.5.2123
更新日期:1986-03-11 00:00:00
abstract::HotSpot Wizard is a web server used for the automated identification of hotspots in semi-rational protein design to give improved protein stability, catalytic activity, substrate specificity and enantioselectivity. Since there are three orders of magnitude fewer protein structures than sequences in bioinformatic datab...
journal_title:Nucleic acids research
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更新日期:2018-07-02 00:00:00
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更新日期:1989-05-25 00:00:00
abstract::Multiplexed tandem PCR (MT-PCR) is a process for highly multiplexed gene expression profiling. In the first step, multiple primer pairs are added to the RNA to be analysed together with reverse transcriptase and Taq DNA polymerase. Following reverse transcription, the multiplexed amplicons are simultaneously amplified...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gni182
更新日期:2005-11-24 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/12.8.3649
更新日期:1984-04-25 00:00:00
abstract::Present results provide direct evidence of the nature of a conformational change in DNA when nucleosomes are formed from core histones and poly [d(A-T)]. First, we have found some features which have characteristic aspects of the A like conformation of DNA. Thus, an increased contribution due to a sugar conformation c...
journal_title:Nucleic acids research
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更新日期:1981-10-10 00:00:00
abstract::Oxoguanine DNA glycosylase (OGG1) initiates the repair of 8-oxoguanine (8-oxoG), a major oxidative DNA base modification that has been directly implicated in cancer and aging. OGG1 functions in the base excision repair pathway, for which a molecular hand-off mechanism has been proposed. To date, only one functional an...
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更新日期:2005-06-07 00:00:00
abstract::The nucleotide sequence of the faeD gene of Escherichia coli and the amino acid sequence of its product is presented. The faeD product is an outer membrane protein required for transport of K88ab fimbrial subunits across the outer membrane. The protein is synthesized as a precursor containing a signal peptide, and the...
journal_title:Nucleic acids research
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更新日期:1986-03-25 00:00:00
abstract::We determined the structure around the gap region between 28S alpha and 28S beta rRNA of Bombyx mori, a lepidopteran insect, to know the introduction mechanism of the hidden break, an interruption of the phosphodiester bond specific to the 28S rRNA of protostomes. Sequence analysis and S1 nuclease mapping suggested th...
journal_title:Nucleic acids research
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更新日期:1986-08-26 00:00:00
abstract::Computational modelling has become increasingly common in life science research. To provide a platform to support universal sharing, easy accessibility and model reproducibility, BioModels (https://www.ebi.ac.uk/biomodels/), a repository for mathematical models, was established in 2005. The current BioModels platform ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkz1055
更新日期:2020-01-08 00:00:00
abstract::Purified UvrA, UvrB, UvrC, UvrD, PolA and Lig proteins from Escherichia coli have been used to assess the effect of nucleotide excision repair on the conformation of native negatively supercoiled plasmid DNA in an in vitro test system. The analysis of labeled reaction products on specific gel systems suggests that the...
journal_title:Nucleic acids research
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更新日期:1989-12-25 00:00:00
abstract::The main goal of this review is to provide some guide-lines for choosing a proper method when searching for differentially expressed transcripts. The choice of an approach should depend upon particular features of the experiment and project requirements. We outline some general considerations to help decide between di...
journal_title:Nucleic acids research
pub_type: 杂志文章,评审
doi:10.1093/nar/26.24.5537
更新日期:1998-12-15 00:00:00
abstract::The deoxyribose hexanucleoside pentaphosphate (m5dC-dG)3 has been studied by 500 MHz 1H NMR in D2O (0.1 M NaCl) and in D2O/deuterated methanol mixtures. Two conformations, in slow equilibrium on the NMR time scale, were detected in methanolic solution. Two-dimensional nuclear Overhauser effect (NOE) experiments were u...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/12.2.1243
更新日期:1984-01-25 00:00:00
abstract::The EcoRV DNA-(adenine-N(6))-methyltransferase (M.EcoRV) specifically modifies the first adenine residue within GATATC sequences. During catalysis, the enzyme flips its target base out of the DNA helix and binds it into a target base binding pocket which is formed in part by Lys16 and Tyr196. A cytosine residue is acc...
journal_title:Nucleic acids research
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更新日期:2001-08-01 00:00:00
abstract::Microsatellites, such as (TG)n found at random throughout the genome, or as 3' extensions of Alu sequences are being increasingly used as genetic markers because of their pluriallelic character. The search for polymorphic microsatellites is time consuming, however, as it is necessary to sequence clones containing the ...
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doi:10.1093/nar/20.6.1333
更新日期:1992-03-25 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2003-07-15 00:00:00
abstract::By subtractive screening of a library made from mRNA of lipopolysaccharide (LPS)-stimulated mouse B lymphocytes we isolated cDNA-clones encoding the ribosomal protein L7. Human L7 mRNA was cloned from activated T-lymphocytes. Although no specific function of L7 in the translation apparatus is known as yet, it should b...
journal_title:Nucleic acids research
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更新日期:1993-01-25 00:00:00