Abstract:
:DNA synthesized by in vitro transcription on rat interferon (IFN) mRNA has been cloned and amplified as recombinant DNA. The nucleotide sequence of these rat IFN cDNA clones revealed i. the partial presence of the coding region of the gene and ii all cDNA clones were derived from the same subtype of rat IFN-alpha mRNA. Purified inserted fragments were used as a hybridisation probe against chromosomal "Southern blots" to show that at least twelve rat IFN-alpha-related sequences are present in the genome. A lambda-linked rat gene library was screened with the cDNA probes, resulting in an equivalent number of rat IFN-alpha-related hybrid phages. By use of a 3'-noncoding region as a probe, the chromosomal counterpart of the cDNA clones could be detected and the nucleotide sequence of its coding region has been determined. Expression of the coding region in E. coli yielded biologically active IFN, when tested for in vitro or in vivo antiviral activity.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Dijkema R,Pouwels P,de Reus A,Schellekens Hdoi
10.1093/nar/12.2.1227subject
Has Abstractpub_date
1984-01-25 00:00:00pages
1227-42issue
2eissn
0305-1048issn
1362-4962journal_volume
12pub_type
杂志文章abstract::Phage display technology involves the display of proteins or peptides, as coat protein fusions, on the surface of a phage or phagemid particles. Using standard technology, helper phage are essential for the replication and assembly of phagemid particles, during library production and biopanning. We have eliminated the...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl772
更新日期:2006-01-01 00:00:00
abstract::A long-range physical map of the centromeric region of human chromosome 7 has been constructed in order to define the region containing sequences with potential involvement in centromere function. The map is centered around alpha satellite DNA, a family of tandemly repeated DNA forming arrays of hundreds to thousands ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/19.9.2295
更新日期:1991-05-11 00:00:00
abstract::We have developed an end-labelling approach to map the positions of nucleosomes and protein binding sites at nucleotide resolution by footprinting micrococcal nuclease (MNase)-sensitive sites. Using this approach we determined that the MFA2 gene and its upstream control regions have four positioned nucleosomes when tr...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/29.13.e64
更新日期:2001-07-01 00:00:00
abstract::We have synthesized a family of double-stranded cDNAs (ds cDNAs) using as a template the family of highly purified protamine mRNAs from rainbow trout testis. Individual pure protamine cDNA components were isolated by cloning this family of protamine ds cDNAs in a plasmid vector (pMB9). Clones containing protamine sequ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/9.6.1463
更新日期:1981-03-25 00:00:00
abstract::The nucleotide sequence of the portion of a Bacillus subtilis (strain PAP115) 3 kb Pst I fragment which contains an endo-beta-1, 4-glucanase gene has been determined. This gene encodes a protein of 499 amino acid residues (Mr = 55,234) with a typical B. subtilis signal peptide. Escherichia coli which has been transfor...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/14.22.9159
更新日期:1986-11-25 00:00:00
abstract::In many eukaryotic mRNAs one or more short 'upstream' open reading frames, uORFs, precede the initiator of the main coding sequence. Upstream ORFs are functionally diverse as illustrated by their variety of features in polyamine pathway biosynthetic mRNAs. Their propensity to act as sensors for regulatory circuits and...
journal_title:Nucleic acids research
pub_type: 杂志文章,评审
doi:10.1093/nar/gkp1037
更新日期:2010-01-01 00:00:00
abstract::The triphosphate of the nucleoside deoxyribosyl dihydropyrimido[4,5-c][1,2]oxazin-7-one (dP) is known to be incorporated into DNA efficiently by Taq polymerase and is a useful tool for polymerase-mediated in vitro mutagenesis. It is shown here that dP is a potent mutagen in Escherichia coli and Salmonella typhimurium ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/25.8.1548
更新日期:1997-04-15 00:00:00
abstract::Spliceosomes are assembled in stages. The first stage forms complex E, which is characterized by the presence of U1 snRNPs base-paired to the 5' splice site, components recognizing the 3' splice site and proteins thought to connect them. The splice sites are held in close proximity and the pre-mRNA is committed to spl...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks322
更新日期:2012-08-01 00:00:00
abstract::The recognition of human tRNA(Leu) or tRNA(Ser) by cognate aminoacyl- tRNA synthetases has distinct requirements. Only one base change (A73-->G) in tRNA(Leu) is required to generate an efficient serine acceptor in vitro, whereas several changes in three structural domains (the acceptor stem, DHU loop and long extra ar...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/24.3.405
更新日期:1996-02-01 00:00:00
abstract::Stability of aromatic phosphoramidates was studied using 2',3'-O-dibenzoyluridine 5'-phosphoramidates and N,2',3'-O-tribenzoylcytidine 5'-phosphate. The effect of dicyclohexylcarbodiimide in this mixture was investigated. Decomposition of the anilidate was slower in the presence of DCC. Substituted anilidates of uridi...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/1.2.223
更新日期:1974-02-01 00:00:00
abstract::We attempted to use the polymerase chain reaction (PCR) to monitor in vitro recombination in a plasmid containing directly repeated sequences. Some of the plasmid preparations which had not been exposed to recombination conditions were however found to behave in the PCR test as if they had undergone homologous recombi...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/19.9.2423
更新日期:1991-05-11 00:00:00
abstract::5' strand resection at DNA double strand breaks (DSBs) is critical for homologous recombination (HR) and genomic stability. Here we develop a novel method to quantitatively measure single-stranded DNA intermediates in human cells and find that the 5' strand at endonuclease-generated break sites is resected up to 3.5 k...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt1309
更新日期:2014-02-01 00:00:00
abstract::Currently transgenic frog embryos are generated using restriction-enzyme-mediated integration (REMI) on decondensed sperm nuclei followed by nuclear transplantation into unfertilized eggs. We have developed a simplified version of this protocol that has the potential to increase the numbers of normally developing tran...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/28.4.e12
更新日期:2000-02-15 00:00:00
abstract::The infection of Pseudomonas aeruginosa by the giant bacteriophage phiKZ is resistant to host RNA polymerase (RNAP) inhibitor rifampicin. phiKZ encodes two sets of polypeptides that are distantly related to fragments of the two largest subunits of cellular multisubunit RNAPs. Polypeptides of one set are encoded by mid...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv1095
更新日期:2015-12-02 00:00:00
abstract::GenBank (R) is a comprehensive sequence database that contains publicly available DNA sequences for more than 119 000 different organisms, obtained primarily through the submission of sequence data from individual laboratories and batch submissions from large-scale sequencing projects. Most submissions are made using ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg057
更新日期:2003-01-01 00:00:00
abstract::Mutations help us to understand the molecular origins of diseases. Researchers, therefore, both publish and seek disease-relevant mutations in public databases and in scientific literature, e.g. Medline. The retrieval tends to be time-consuming and incomplete. Automated screening of the literature is more efficient. W...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkh162
更新日期:2004-01-02 00:00:00
abstract::Nucleotide excision DNA repair is mechanistically conserved across all kingdoms of life. In prokaryotes, this multi-enzyme process requires six proteins: UvrA-D, DNA polymerase I and DNA ligase. To examine how UvrC locates the UvrB-DNA pre-incision complex at a site of damage, we have labeled UvrB and UvrC with differ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt177
更新日期:2013-05-01 00:00:00
abstract::The Class 3 aldehyde dehydrogenase gene (ALDH-3) is differentially expressed. Expression is either constitutive or xenobiotic inducible via an aromatic hydrocarbon (Ah) receptor-mediated pathway, depending upon the tissue. A series of studies were performed to examine the regulation of rat ALDH-3 basal expression. DNa...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/24.21.4185
更新日期:1996-11-01 00:00:00
abstract::Human T-cell leukemia virus type 1 (HTLV-1) is the etiological agent of adult T-cell leukemia (ATL). The HTLV-1 basic leucine zipper protein (HBZ) is expressed in all cases of ATL and is directly associated with virus pathogenicity. The two isoforms of the HBZ protein are synthesized from antisense messenger RNAs (mRN...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky802
更新日期:2018-11-16 00:00:00
abstract::We have developed an information repository named SpliceInfo to collect the occurrences of the four major alternative-splicing (AS) modes in human genome; these include exon skipping, 5'-alternative splicing, 3'-alternative splicing and intron retention. The dataset is derived by comparing the nucleotide and protein s...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gki129
更新日期:2005-01-01 00:00:00
abstract::Several modified nucleosides were introduced during in vitro RNA synthesis into a pre-tRNA(Ser). The pre-tRNAs were used as substrates for RNase P enzymes. No effects were observed with biotin-8-ATP or [alpha-S]-GPT, whereas with m7GTP, the cleavage reaction was completely inhibited. Analysis of pre-tRNAs which contai...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/18.4.837
更新日期:1990-02-25 00:00:00
abstract::The protein property prediction and testing database (PPT-DB) is a database housing nearly 30 carefully curated databases, each of which contains commonly predicted protein property information. These properties include both structural (i.e. secondary structure, contact order, disulfide pairing) and dynamic (i.e. orde...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm800
更新日期:2008-01-01 00:00:00
abstract::Gene duplications are a major source of evolutionary innovations. Understanding the functional divergence of duplicates and their role in genetic robustness is an important challenge in biology. Previously, analyses of genetic robustness were primarily focused on duplicates essentiality and epistasis in several labora...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt1200
更新日期:2014-02-01 00:00:00
abstract::The pDd56 mRNA sequence is highly enriched in prestalk over prespore cells and is inducible by DIF, the putative Dictyostelium stalk-specific morphogen. We show that the pDd56 gene is composed of forty one copies of a twenty four amino acid, cysteine rich repeat. This is highly homologus to a repeat which we have prev...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/15.18.7463
更新日期:1987-09-25 00:00:00
abstract::Post-translational modifications (such as ubiquitination) of clock proteins are critical in maintaining the precision and robustness of the evolutionarily conserved circadian clock. Ubiquitination of the core clock transcription factor BMAL1 (brain and muscle Arnt-like 1) has recently been reported. However, it remain...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gku225
更新日期:2014-05-01 00:00:00
abstract::Eucaryotic transcription initiation by RNA polymerase II involves protein:DNA interactions during the formation of a transcription complex. In addition to RNA polymerase II there are at least five other general transcription factors necessary for initiation with the adenovirus major late promoter. One of these, TFIIA,...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/18.12.3611
更新日期:1990-06-25 00:00:00
abstract::Carbohydrate-binding proteins play crucial roles across all organisms and viruses. The complexity of carbohydrate structures, together with inconsistencies in how their 3D structures are reported, has led to difficulties in characterizing the protein-carbohydrate interfaces. In order to better understand protein-carbo...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkz860
更新日期:2020-01-08 00:00:00
abstract::Methyl carbon-13 NMR spectra of purified tRNA species are presented for the first time. In addition, these spectra of tRNA species specific for phenylalanine, tyrosine, and cysteine exhibited the first resolution of single methyl carbon resonances. Carbon-13 enriched methyl groups of ribothymidine (T) and 7-methylguan...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/8.9.2085
更新日期:1980-05-10 00:00:00
abstract::The T box transcription antitermination regulatory system, found in Gram-positive bacteria, is dependent on a complex set of interactions between uncharged tRNA and the 5'-untranslated mRNA leader region of the regulated gene. One of these interactions involves the base pairing of the acceptor end of cognate tRNA with...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gki546
更新日期:2005-05-06 00:00:00
abstract::RNA polymerase I-catalyzed synthesis of the Schizosaccharomyces pombe approximately 37S pre-rRNAs was shown to be sensitive to regulatory sequences located several kilobases upstream of the initiation site for the rRNA gene. An in vitro transcription system for RNA polymerase I-catalyzed RNA synthesis was established ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/25.3.659
更新日期:1997-02-01 00:00:00