Abstract:
:5' strand resection at DNA double strand breaks (DSBs) is critical for homologous recombination (HR) and genomic stability. Here we develop a novel method to quantitatively measure single-stranded DNA intermediates in human cells and find that the 5' strand at endonuclease-generated break sites is resected up to 3.5 kb in a cell cycle-dependent manner. Depletion of CtIP, Mre11, Exo1 or SOSS1 blocks resection, while depletion of 53BP1, Ku or DNA-dependent protein kinase catalytic subunit leads to increased resection as measured by this method. While 53BP1 negatively regulates DNA end processing, depletion of Brca1 does not, suggesting that the role of Brca1 in HR is primarily to promote Rad51 filament formation, not to regulate end resection.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Zhou Y,Caron P,Legube G,Paull TTdoi
10.1093/nar/gkt1309subject
Has Abstractpub_date
2014-02-01 00:00:00pages
e19issue
3eissn
0305-1048issn
1362-4962pii
gkt1309journal_volume
42pub_type
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