Abstract:
:Eucaryotic transcription initiation by RNA polymerase II involves protein:DNA interactions during the formation of a transcription complex. In addition to RNA polymerase II there are at least five other general transcription factors necessary for initiation with the adenovirus major late promoter. One of these, TFIIA, is involved in the earliest events during transcription complex assembly. We have purified TFIIA from wheat germ and characterized it in an in vitro transcription system. Wheat TFIIA is a single polypeptide of Mr approximately 35 kd which functionally replaces human (HeLa) TFIIA to form a wheat/HeLa transcription system. [This polypeptide can be eluted from a SDS-polyacrylamide gel, refolded to a native conformation, and will function as wheat TFIIA in the heterologous system.] The heterologous system requires a lower optimal incubation temperature than the HeLa system. Biochemical characterization, using the adenovirus major late promoter, indicates that transcription reaction parameters for both wheat and HeLa TFIIA are similar but the kinetics of transcription for both TFIIAs are somewhat dissimilar. A plant viral promoter, the cauliflower mosaic virus 35S promoter, accurately and efficiently directs in vitro transcription in both the wheat/HeLa and HeLa systems with identical transcription kinetics. We conclude that TFIIA function has been conserved during evolution.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Burke C,Yu XB,Marchitelli L,Davis EA,Ackerman Sdoi
10.1093/nar/18.12.3611subject
Has Abstractpub_date
1990-06-25 00:00:00pages
3611-20issue
12eissn
0305-1048issn
1362-4962journal_volume
18pub_type
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journal_title:Nucleic acids research
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abstract::Trinucleotide exchange (TriNEx) is a method for generating novel molecular diversity during directed evolution by random substitution of one contiguous trinucleotide sequence for another. Single trinucleotide sequences were deleted at random positions in a target gene using the engineered transposon MuDel that were su...
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journal_title:Nucleic acids research
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doi:10.1093/nar/gkl513
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journal_title:Nucleic acids research
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doi:10.1093/nar/24.18.3538
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abstract::NELF and DSIF act together to inhibit transcription elongation in vitro, and are implicated in causing promoter proximal pausing on the hsp70 gene in Drosophila. Here, further characterization of Drosophila NELF is provided. Drosophila NELF has four subunits similar to subunits of human NELF. The amino acid sequences ...
journal_title:Nucleic acids research
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更新日期:2005-03-01 00:00:00
abstract::Mutator-like transposable elements (MULEs) are widespread across fungal, plant and animal species. Despite their abundance and importance as genetic tools in plants, the transposition mechanism of the MULE superfamily was previously unknown. Discovery of the Muta1 element from Aedes aegypti and its successful transpos...
journal_title:Nucleic acids research
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/30.11.2508
更新日期:2002-06-01 00:00:00
abstract::Interferons (IFNs) are a family of multifunctional proteins involved in immune activation, regulation of cell growth and antiviral response. They exert their functions by induction of several IFN-stimulated genes, including IFN regulatory factors (IRFs), a family of transcriptional regulators. One of these factors, IR...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/28.21.4219
更新日期:2000-11-01 00:00:00
abstract::This is a study of the effect of total and partial deuteration of solvent on critical temperatures and profiles of all four reactions occurring in poly(A) x n poly(U) (n = 1 or 2) aqueous systems. The study was done at observational times not longer than hydrogen exchange times at base pairs in helically ordered struc...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/8.18.4283
更新日期:1980-09-25 00:00:00
abstract::To explain the activation of transcription by a remote enhancer, two models are most often considered, namely looping and scanning. A scanning model, also referred to as 'polymerase entry site' model predicts that for two adjacent promoters the one proximal to an enhancer would be preferentially activated. Preferentia...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/17.22.8931
更新日期:1989-11-25 00:00:00
abstract::A mass culture of human diploid fibroblasts, and eight clones isolated from that mass culture, were examined for methylation patterns in several regions of DNA. Plasmid-inserted cDNA sequences were used as probes for alpha-hCG, beta-globin, A gamma- and G gamma-globin, and beta- and gamma-actin gene regions. Each prob...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/10.14.4293
更新日期:1982-07-24 00:00:00
abstract::Analysis of large-scale gene expression studies usually begins with gene clustering. A ubiquitous problem is that different algorithms applied to the same data inevitably give different results, and the differences are often substantial, involving a quarter or more of the genes analyzed. This raises a series of import...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gki536
更新日期:2005-05-10 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/18.8.2133
更新日期:1990-04-25 00:00:00
abstract::An 11 S ribonucleoprotein particle was isolated from maize endosperm and shown to be functionally and structurally equivalent to the mammalian signal recognition particle. However, unlike animal cells which apparently contain a single 7 SL RNA species, maize endosperm contains a heterogeneous population of 7 SL RNA. T...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/17.4.1573
更新日期:1989-02-25 00:00:00
abstract::Phage_Finder, a heuristic computer program, was created to identify prophage regions in completed bacterial genomes. Using a test dataset of 42 bacterial genomes whose prophages have been manually identified, Phage_Finder found 91% of the regions, resulting in 7% false positive and 9% false negative prophages. A searc...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl732
更新日期:2006-01-01 00:00:00
abstract::A typical G-rich telomeric DNA strand, which runs 5'-->3' toward the chromosome ends, protrudes by several nucleotides in lower eukaryotes. In human chromosomes long G-rich 3'-overhangs have been found. Apart from the standard G-rich tail, several non-canonical terminal structures have been proposed. However, the mech...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/29.7.e35
更新日期:2001-04-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky987
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abstract::Pulsed field gel electrophoresis was used to determine the location of the tumour necrosis factor (TNF) alpha and beta genes. They were shown to be linked to the HLA-B locus; analogous to their location in mouse, between the complement (class III) region and H-2D. However, the distance between the TNF genes and the cl...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/15.21.8957
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journal_title:Nucleic acids research
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更新日期:2003-09-01 00:00:00
abstract::The avian retrovirus pp32 DNA endonuclease prefers to nick supercoiled DNA containing long terminal repeat (LTR) circle junction sequences at one or the other of two sites, each which mapped two nucleotides back from the circle junction. The sequence at the sites of nicking was (sequence: see text) where increases ind...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/13.17.6205
更新日期:1985-09-11 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/6.4.1709
更新日期:1979-04-01 00:00:00
abstract::Base specificity in the interaction of ethidium with double stranded synthetic RNA homopolymers has been studied by means of spectroscopic (UV-visible absorption and fluorescence), microcalorimetric and dilatometric techniques. The results show a strong base specificity in this interaction, the association constant wi...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/15.14.5803
更新日期:1987-07-24 00:00:00
abstract::Protein-DNA recognition is a critical component of gene regulatory processes but the underlying molecular mechanisms are not yet completely understood. Whereas the DNA binding preferences of transcription factors (TFs) are commonly described using nucleotide sequences, the 3D DNA structure is recognized by proteins an...
journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2014-01-01 00:00:00
abstract::Hybridization solutions containing chaotropes may be used to modulate the thermal stability (Tm or Td) of oligodeoxynucleotide (ODN) duplexes or hybrids over a 90 degrees C range. Modulation of Td allows formulation of hybridization solutions that permit ambient temperature hybridization using most combinations of pro...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/19.19.5143
更新日期:1991-10-11 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/12.21.8181
更新日期:1984-11-12 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1996-09-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2006-02-09 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2019-06-04 00:00:00
abstract::In this paper we report the characterization of a newly sequenced 5' upstream region of the human HLA-DRA gene. We performed (i) search for transcription factor motifs, (ii) analysis of CpG display and observed/expected frequency ratios, (iii) search for regions homologous to the 5' upstream sequences of the murine EA...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/23.10.1671
更新日期:1995-05-25 00:00:00