Abstract:
:In this paper we report the characterization of a newly sequenced 5' upstream region of the human HLA-DRA gene. We performed (i) search for transcription factor motifs, (ii) analysis of CpG display and observed/expected frequency ratios, (iii) search for regions homologous to the 5' upstream sequences of the murine EA gene, (iv) DNase I footprinting experiments and (v) electrophoretic mobility shift assays. Our results demonstrate the existence, in the HLA-DRA gene, of Y' and X' boxes highly homologous to the Y and X boxes present in MHC class II genes, but oriented in the opposite direction. These Y' and X' boxes have been conserved during the molecular evolution of both human HLA-DRA and murine EA genes. DNase I footprinting and gel retardation experiments suggest that the X' and Y' boxes of the HLA-DRA upstream gene region are specifically recognized by nuclear proteins that also bind to the X and Y boxes of the HLA-DRA proximal promoter, respectively.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Feriotto G,Mischiati C,Bianchi N,Rutigliano C,Giacomini P,Gambari Rdoi
10.1093/nar/23.10.1671subject
Has Abstractpub_date
1995-05-25 00:00:00pages
1671-8issue
10eissn
0305-1048issn
1362-4962pii
5c0031journal_volume
23pub_type
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