4'-Acylated thymidine 5'-triphosphates: a tool to increase selectivity towards HIV-1 reverse transcriptase.

abstract：:We have developed a web server, PRince, which analyzes the structural features and physicochemical properties of the protein-RNA interface. Users need to submit a PDB file containing the atomic coordinates of both the protein and the RNA molecules in complex form (in '.pdb' format). They should also mention the chain ...

journal_title：Nucleic acids research

authors： Barik A,Mishra A,Bahadur RP

更新日期：2012-07-01 00:00:00

abstract：:Two novel fluorescent cyanine-AMP conjugates, F550/570 and F650/670, have been synthesized to serve as transcription initiators under the T7 phi2.5 promoter. Efficient fluorophore labeling of 5' RNA is achieved in a single transcription step by including F550/570 and F650/670 in the transcription solution. The current...

journal_title：Nucleic acids research

authors： Li N,Yu C,Huang F

更新日期：2005-02-24 00:00:00

abstract：:We have analyzed the DNaseI sensitivity of chromatin from the rat albumin and alpha-fetoprotein genes in the fetal liver (which synthesizes albumin and alpha-fetoprotein), adult liver (which synthesizes albumin), fetal yolk sac (which synthesizes alpha-fetoprotein), and adult kidney (which synthesizes neither). Active...

journal_title：Nucleic acids research

authors： Kunnath L,Locker J

更新日期：1985-01-11 00:00:00

abstract：:MicroRNA (miRNA) 5'-isoforms, or 5'-isomiRs, are small-RNA species that originate from the same genomic loci as the major miRNAs with their 5' ends shifted from the 5' ends of the miRNAs by a few nucleotides. Although 5'-isomiRs have been reported, their origins, properties and potential functions remain to be examine...

journal_title：Nucleic acids research

authors： Xia J,Zhang W

更新日期：2014-02-01 00:00:00

abstract：:Minigenes encoding the peptide Met-Arg-Arg have been used to study the mechanism of toxicity of AGA codons proximal to the start codon or prior to the termination codon in bacteria. The codon sequences of the 'mini-ORFs' employed were initiator, combinations of AGA and CGA, and terminator. Both, AGA and CGA are low-us...

journal_title：Nucleic acids research

authors： Cruz-Vera LR,Magos-Castro MA,Zamora-Romo E,Guarneros G

更新日期：2004-08-18 00:00:00

abstract：:RNA can directly bind to purine-rich DNA via Hoogsteen base pairing, forming a DNA:RNA triple helical structure that anchors the RNA to specific sequences and allows guiding of transcription regulators to distinct genomic loci. To unravel the prevalence of DNA:RNA triplexes in living cells, we have established a fast ...

journal_title：Nucleic acids research

authors： Sentürk Cetin N,Kuo CC,Ribarska T,Li R,Costa IG,Grummt I

更新日期：2019-03-18 00:00:00

abstract：:We have previously reported the isolation of mammalian cell lines expressing the 3-methyladenine DNA glycosylase I (tag) gene from E. coli. These cells are 2-5 fold more resistant to the toxic effects of methylating agents than normal cells (15). Kinetic measurements of 3-methyladenine removal from the genome in situ ...

journal_title：Nucleic acids research

authors： Klungland A,Bjørås M,Hoff E,Seeberg E

更新日期：1994-05-11 00:00:00

abstract：:The mutL and mutS genes of Vibrio cholerae have been identified using interspecific complementation of Escherichia coli mutL and mutS mutants with plasmids containing the gene bank of V. cholerae. The recombinant plasmid pJT470, containing a 4.7 kb fragment of V. cholerae DNA codes for a protein of molecular weight 92...

journal_title：Nucleic acids research

authors： Bera TK,Ghosh SK,Das J

更新日期：1989-08-11 00:00:00

abstract：:The gene encoding myelin basic protein (MBP) contains multiple activator sequences spanning upstream of its transcriptional initiation site which differentially promote transcription in glial cells. The proximal activator sequence, designated MB1, activates transcription in a glial cell type specific manner. This sequ...

journal_title：Nucleic acids research

authors： Devine-Beach K,Haas S,Khalili K

更新日期：1992-02-11 00:00:00

abstract：:The National Center for Biotechnology Information (NCBI) provides a large suite of online resources for biological information and data, including the GenBank® nucleic acid sequence database and the PubMed database of citations and abstracts for published life science journals. The Entrez system provides search and re...

journal_title：Nucleic acids research

authors： NCBI Resource Coordinators.

更新日期：2018-01-04 00:00:00

abstract：:Oligoribonucleotides with 2'-5' linkages have been synthesized on solid support. UV melting and CD experiments indicate complementary strands associate to give complexes with melting temperatures 30 to 40 degrees C lower than for duplexes formed by 3'-5' oligoribonucleotides with the same sequence. UV melting and imin...

journal_title：Nucleic acids research

authors： Kierzek R,He L,Turner DH

更新日期：1992-04-11 00:00:00

abstract：:Restriction-free cloning (RF-cloning) is a PCR-based technology that expands on the QuikChange™ mutagenesis process originally popularized by Stratagene in the mid-1990s, and allows the insertion of essentially any sequence into any plasmid at any location. While RF-cloning is a powerful tool for the design of custom ...

journal_title：Nucleic acids research

authors： Bond SR,Naus CC

更新日期：2012-07-01 00:00:00

abstract：:Many strains of E. coli K12 restrict DNA containing cytosine methylation such as that present in plant and animal genomes. Such restriction can severely inhibit the efficiency of cloning genomic DNAs. We have quantitatively evaluated a total of 39 E. coli strains for their tolerance to cytosine methylation in phage an...

journal_title：Nucleic acids research

authors： Woodcock DM,Crowther PJ,Doherty J,Jefferson S,DeCruz E,Noyer-Weidner M,Smith SS,Michael MZ,Graham MW

更新日期：1989-05-11 00:00:00

abstract：:We have developed a novel method of genetic library construction on magnetic microbeads based on solid-phase single-molecule PCR in a fine and robust water-phase compartment formed in water-in-oil (w/o) emulsions. In this method, critically diluted DNA fragments were distributed over the emulsion as templates, where b...

journal_title：Nucleic acids research

authors： Kojima T,Takei Y,Ohtsuka M,Kawarasaki Y,Yamane T,Nakano H

更新日期：2005-10-06 00:00:00

abstract：:Ribosome-inactivating proteins (RIPs) inhibit protein synthesis by enzymatically depurinating a specific adenine residue at the sarcin-ricin loop of the 28S rRNA, which thereby prevents the binding of elongation factors to the GTPase activation centre of the ribosome. Here, we present the 2.2 A crystal structure of tr...

journal_title：Nucleic acids research

authors： Too PH,Ma MK,Mak AN,Wong YT,Tung CK,Zhu G,Au SW,Wong KB,Shaw PC

更新日期：2009-02-01 00:00:00

abstract：:Ribosomal DNA (rDNA) from Xenopus laevis contains several heterogeneities in all three transcribed spacers, as revealed by analysis of cloned and uncloned amplified rDNA from oocytes and cloned chromosomal rDNA from erythrocytes. Heterogeneities include single base changes and length variants of one to several nucleot...

journal_title：Nucleic acids research

authors： Stewart MA,Hall LM,Maden BE

更新日期：1983-02-11 00:00:00

abstract：:Programmed ribosomal frameshifting provides a mechanism to decode information located in two overlapping reading frames by diverting a proportion of translating ribosomes into a second open reading frame (ORF). The result is the production of two proteins: the product of standard translation from ORF1 and an ORF1-ORF2...

journal_title：Nucleic acids research

authors： Henderson CM,Anderson CB,Howard MT

更新日期：2006-01-01 00:00:00

abstract：:Human adenovirus mutants that carry a large deletion in early region 4 (E4) are severely defective in the synthesis of viral late proteins. Plasmids that carry intact E4 sequences can complement the late protein synthetic defect of such mutants when introduced into infected cells by transfection, presumably due to the...

journal_title：Nucleic acids research

authors： Ketner G,Bridge E,Virtanen A,Hemström C,Pettersson U

更新日期：1989-04-25 00:00:00

abstract：:A method is presented for the purification of mRNAs and the identification of structural gene sequences in recombinant DNA molecules. RNA is hybridized to double-stranded linear DNA such that R-loops are formed between most DNAs and their complementary RNA sequences. These R-loops are purified from unhybridized RNAs b...

journal_title：Nucleic acids research

authors： Woolford JL Jr,Rosbash M

更新日期：1979-06-11 00:00:00

abstract：:Neph et al. (2012) (Circuitry and dynamics of human transcription factor regulatory networks. Cell, 150: 1274-1286) reported the transcription factor (TF) regulatory networks of 41 human cell types using the DNaseI footprinting technique. This provides a valuable resource for uncovering regulation principles in differ...

journal_title：Nucleic acids research

authors： Zhang S,Tian D,Tran NH,Choi KP,Zhang L

更新日期：2014-11-10 00:00:00

abstract：:Expressed polycistronic microRNA (miR) cassettes have useful properties that can be utilized for RNA interference (RNAi)-based gene silencing. To advance their application we generated modular trimeric anti-hepatitis B virus (HBV) Pol II cassettes encoding primary (pri)-miR-31-derived shuttles that target three differ...

journal_title：Nucleic acids research

authors： Ely A,Naidoo T,Arbuthnot P

更新日期：2009-07-01 00:00:00

abstract：:RNA silencing is an evolutionarily conserved mechanism triggered by double-stranded RNA that is processed into 21- to 24-nt small interfering (si)RNA or micro (mi)RNA by RNaseIII-like enzymes called Dicers. Gene regulations by RNA silencing have fundamental implications in a large number of biological processes that i...

journal_title：Nucleic acids research

authors： Uddin MN,Dunoyer P,Schott G,Akhter S,Shi C,Lucas WJ,Voinnet O,Kim JY

更新日期：2014-07-01 00:00:00

abstract：:Protein domains are subunits of proteins that recur throughout the protein world. There are many definitions attempting to capture the essence of a protein domain, and several systems that identify protein domains and classify them into families. EVEREST, recently described in Portugaly et al. (2006) BMC Bioinformatic...

journal_title：Nucleic acids research

authors： Portugaly E,Linial N,Linial M

更新日期：2007-01-01 00:00:00

abstract：:The E. coli single-stranded binding protein (SSB) has been demonstrated in vitro to be involved in a number of replicative, DNA renaturation, and protective functions. It was shown previously that SSB can interact with exonuclease I to stimulate the hydrolysis of single-stranded DNA. We demonstrate here that E. coli S...

journal_title：Nucleic acids research

authors： Sandigursky M,Franklin WA

更新日期：1994-01-25 00:00:00

abstract：:The channel proteins belonging to the major intrinsic proteins (MIP) superfamily are diverse and are found in all forms of life. Water-transporting aquaporin and glycerol-specific aquaglyceroporin are the prototype members of the MIP superfamily. MIPs have also been shown to transport other neutral molecules and gases...

journal_title：Nucleic acids research

authors： Gupta AB,Verma RK,Agarwal V,Vajpai M,Bansal V,Sankararamakrishnan R

更新日期：2012-01-01 00:00:00

abstract：:pS194 is a naturally occurring Staphylococcus aureus plasmid encoding streptomycin resistance. The plasmid has a copy number of about 25 per cell, and belongs to the inc5 incompatibility group. The nucleotide sequence of pS194 has been determined and consists of 4397 base pairs including four open reading frames poten...

journal_title：Nucleic acids research

authors： Projan SJ,Moghazeh S,Novick RP

更新日期：1988-03-25 00:00:00

abstract：:Existence of some extra-genetic (epigenetic) codes has been postulated since the discovery of the primary genetic code. Evident effects of histone post-translational modifications or DNA methylation over the efficiency and the regulation of DNA processes are supporting this postulation. EMdeCODE is an original algorit...

journal_title：Nucleic acids research

authors： Santoni FA

更新日期：2013-02-01 00:00:00

abstract：:A sensitive gel retention assay has been utilized to detect proteins from uninfected Hela nuclei which interact with the adenovirus type 2 enhancer. This assay has been employed to monitor fractionation of nuclear extracts. Three enhancer binding factors were resolved by chromatography on DEAE-Sepharose and one of the...

journal_title：Nucleic acids research

authors： Barrett P,Clark L,Hay RT

更新日期：1987-03-25 00:00:00

abstract：:The chromatographic profiles of isoaccepting tRNAs were analyzed at five time points during the 96 hr, dimethylsulfoxide induced, erythroid-like differentiation of Friend leukemia cells. Sixty-four isoaccepting species of tRNA for 16 amino acids were resolved by RPC-5 chromatography. The relative amounts of tRNAphe, t...

journal_title：Nucleic acids research

authors： Lin VK,Agris PF

更新日期：1980-08-11 00:00:00

abstract：:Histone H2B O-GlcNAcylation is an important post-translational modification of chromatin during gene transcription. However, how this epigenetic modification is regulated remains unclear. Here we found that the energy-sensing adenosine-monophosphate-activated protein kinase (AMPK) could suppress histone H2B O-GlcNAcyl...

journal_title：Nucleic acids research

authors： Xu Q,Yang C,Du Y,Chen Y,Liu H,Deng M,Zhang H,Zhang L,Liu T,Liu Q,Wang L,Lou Z,Pei H

更新日期：2014-05-01 00:00:00