Abstract:
:Restriction-free cloning (RF-cloning) is a PCR-based technology that expands on the QuikChange™ mutagenesis process originally popularized by Stratagene in the mid-1990s, and allows the insertion of essentially any sequence into any plasmid at any location. While RF-cloning is a powerful tool for the design of custom plasmids when restriction sites are not conveniently situated, manually designing the requisite primers can be tedious and error prone. We present here a web-service that automates the primer design process, along with a user interface that includes a number of useful tools for managing both the input sequences and the resulting outputs. RF-Cloning is free and open to all users, and can be accessed at http://www.rf-cloning.org.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Bond SR,Naus CCdoi
10.1093/nar/gks396subject
Has Abstractpub_date
2012-07-01 00:00:00pages
W209-13issue
Web Server issueeissn
0305-1048issn
1362-4962pii
gks396journal_volume
40pub_type
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