CpG islands of the X chromosome are gene associated.

abstract：:High-throughput screening assays have been developed to rapidly identify small molecule inhibitors targeting catalytic group I introns. Biochemical reactions catalyzed by a self-splicing group I intron derived from Pneumocystis carinii or from bacteriophage T4 have been investigated. In vitro biochemical assays amenab...

journal_title：Nucleic acids research

authors： Mei HY,Cui M,Sutton ST,Truong HN,Chung FZ,Czarnik AW

更新日期：1996-12-15 00:00:00

abstract：:Spliced leader (SL) trans-splicing is a critical element of gene expression in a number of eukaryotic groups. This process is arguably best understood in nematodes, where biochemical and molecular studies in Caenorhabditis elegans and Ascaris suum have identified key steps and factors involved. Despite this, the preci...

journal_title：Nucleic acids research

authors： Philippe L,Pandarakalam GC,Fasimoye R,Harrison N,Connolly B,Pettitt J,Müller B

更新日期：2017-08-21 00:00:00

abstract：:We have constructed a stable cell line, human embryonal kidney 293M+, containing a lacZ reporter gene controlled by an in vitro methylated hormone-responsive enhancer. Methylation of the enhancer-promoter abolishes lacZ expression controlled by ponasterone A (an analogue of ecdysone). Ponasterone A-induced expression ...

journal_title：Nucleic acids research

authors： Benjamin D,Jost JP

更新日期：2001-09-01 00:00:00

abstract：:Functionality of the non-coding transcripts encoded by the human genome is the coveted goal of the modern genomics research. While commonly relied on the classical methods of forward genetics, integration of different genomics datasets in a global Systems Biology fashion presents a more productive avenue of achieving ...

journal_title：Nucleic acids research

authors： St Laurent G,Vyatkin Y,Antonets D,Ri M,Qi Y,Saik O,Shtokalo D,de Hoon MJ,Kawaji H,Itoh M,Lassmann T,Arner E,Forrest AR,FANTOM consortium.,Nicolas E,McCaffrey TA,Carninci P,Hayashizaki Y,Wahlestedt C,Kapranov P

更新日期：2016-04-20 00:00:00

abstract：:We have generated a codon usage table for Pseudomonas aeruginosa. Codon usage in P. aeruginosa is extremely biased. In contrast to E. coli and yeast, P. aeruginosa preferentially uses those codons within a synonymous codon group with the strongest predicted codon-anticodon interaction. We were unable to correlate a pa...

journal_title：Nucleic acids research

authors： West SE,Iglewski BH

更新日期：1988-10-11 00:00:00

abstract：:We present the sequences of five additional human U2 pseudogenes which are very similar to the U2.13 pseudogene reported previously [Van Arsdell et al. (1981) Cell 26, 11-17]. All six U2 pseudogenes preserve the 5' end of the mature U2 snRNA sequence, and all six are flanked by nearly perfect direct repeats that diffe...

journal_title：Nucleic acids research

authors： Van Arsdell SW,Weiner AM

更新日期：1984-02-10 00:00:00

abstract：:We introduce M-Coffee, a meta-method for assembling multiple sequence alignments (MSA) by combining the output of several individual methods into one single MSA. M-Coffee is an extension of T-Coffee and uses consistency to estimate a consensus alignment. We show that the procedure is robust to variations in the choice...

journal_title：Nucleic acids research

authors： Wallace IM,O'Sullivan O,Higgins DG,Notredame C

更新日期：2006-03-23 00:00:00

abstract：:About 40% of the genes in the nematode Caenorhabditis elegans have homologs in humans. Based on the history of this model system, it is clear that the application of genetic methods to the study of this set of genes would provide important clues to their function in humans. To facilitate such genetic studies, we are e...

journal_title：Nucleic acids research

authors： Edgley M,D'Souza A,Moulder G,McKay S,Shen B,Gilchrist E,Moerman D,Barstead R

更新日期：2002-06-15 00:00:00

abstract：:DNA breaks occur during many processes in mammalian cells, including recombination, repair, mutagenesis and apoptosis. Here we report a simple and rapid method for assaying DNA breaks and identifying DNA breaksites. Breaksites are first tagged and amplified by ligation-mediated PCR (LM-PCR), using nested PCR primers t...

journal_title：Nucleic acids research

authors： Kong Q,Maizels N

更新日期：2001-03-15 00:00:00

abstract：:We had shown previously (3) that the E. coli argE gene could be turned-on by an IS3 element inserted in orientation II near the 5' end of the gene. Here we show that this effect is due to the presence of an outward promoter located on IS3. The exact site of insertion of IS3 was determined by DNA sequencing. Using the ...

journal_title：Nucleic acids research

authors： Charlier D,Piette J,Glansdorff N

更新日期：1982-10-11 00:00:00

abstract：:Methylation of miRNAs at the 2'-hydroxyl group on the ribose at 3'-end (2'-O-methylation, 2'Ome) is critical for miRNA function in plants and Drosophila. Whether this methylation phenomenon exists for mammalian miRNA remains unknown. Through LC-MS/MS analysis, we discover that majority of miR-21-5p isolated from human...

journal_title：Nucleic acids research

authors： Liang H,Jiao Z,Rong W,Qu S,Liao Z,Sun X,Wei Y,Zhao Q,Wang J,Liu Y,Chen X,Wang T,Zhang CY,Zen K

更新日期：2020-07-27 00:00:00

abstract：:The prime mechanism by which p53 acts as a tumor suppressor is as a transcription factor regulating the expression of diverse downstream genes. The DNA-binding domain of p53 (p53DBD) interacts with defined DNA sites and is the main target for mutations in human primary tumors. Here, we show that the CWWG motif, found ...

journal_title：Nucleic acids research

authors： Beno I,Rosenthal K,Levitine M,Shaulov L,Haran TE

更新日期：2011-03-01 00:00:00

abstract：:A fraction of the ribosomal 28S genes in some insects are interrupted at specific sites by insertion elements R1 and R2 (also called Type I and II). These elements contain long open-reading frames with homology to reverse transcriptase. We have identified in the silkmoth, Bombyx mori, copies of these elements which ha...

journal_title：Nucleic acids research

authors： Xiong Y,Burke WD,Jakubczak JL,Eickbush TH

更新日期：1988-11-25 00:00:00

abstract：:As the formation of ribonucleoprotein complexes is a major mechanism of angiotensin II type 1 receptor (AT1R) regulation, we sought to identify novel AT1R mRNA binding proteins. By affinity purification and mass spectroscopy, we identified TIA-1. This interaction was confirmed by colocalization of AT1R mRNA and TIA-1 ...

journal_title：Nucleic acids research

authors： Backlund M,Paukku K,Kontula KK,Lehtonen JY

更新日期：2016-04-20 00:00:00

abstract：:GC content bias describes the dependence between fragment count (read coverage) and GC content found in Illumina sequencing data. This bias can dominate the signal of interest for analyses that focus on measuring fragment abundance within a genome, such as copy number estimation (DNA-seq). The bias is not consistent b...

journal_title：Nucleic acids research

authors： Benjamini Y,Speed TP

更新日期：2012-05-01 00:00:00

abstract：:Ribosomes transit between two conformational states, non-rotated and rotated, through the elongation cycle. Here, we present evidence that an internal loop in the essential yeast ribosomal protein rpL10 is a central controller of this process. Mutations in this loop promote opposing effects on the natural equilibrium ...

journal_title：Nucleic acids research

authors： Sulima SO,Gülay SP,Anjos M,Patchett S,Meskauskas A,Johnson AW,Dinman JD

更新日期：2014-02-01 00:00:00

abstract：:As an approach to gene therapy for the respiratory manifestations of cystic fibrosis (CF), in vivo plasmid-mediated direct transfer of the normal CF transmembrane conductance regulator (CFTR) gene to the airway epithelium was investigated in mice. To evaluate the feasibility of this strategy, pRSVL, a plasmid composed...

journal_title：Nucleic acids research

authors： Yoshimura K,Rosenfeld MA,Nakamura H,Scherer EM,Pavirani A,Lecocq JP,Crystal RG

更新日期：1992-06-25 00:00:00

abstract：:In the field of RNA, the technologies for studying the transcriptome have created a tremendous potential for deciphering the puzzles of the RNA biology. Along with the excitement, the unprecedented volume of RNA related omics data is creating great challenges in bioinformatics analyses. Here, we present the RNA Centri...

journal_title：Nucleic acids research

authors： Uyar B,Yusuf D,Wurmus R,Rajewsky N,Ohler U,Akalin A

更新日期：2017-06-02 00:00:00

abstract：:Mouse DNA contains two equally abundant, homologous subfamilies of MspI 3.6 and 5 kb repeated fragments. The first subfamily corresponds to the previously described (1) Bam 4 kb repeats, the second one to Bam repeated fragments of higher molecular weight. These subfamilies account for the vast majority of long Bam rep...

journal_title：Nucleic acids research

authors： Meunier-Rotival M,Bernardi G

更新日期：1984-02-10 00:00:00

abstract：:Pairing of a given E3 ubiquitin ligase with different E2s allows synthesis of ubiquitin conjugates of different topologies. While this phenomenon contributes to functional diversity, it remains largely unknown how a single E3 ubiquitin ligase recognizes multiple E2s, and whether identical structural requirements deter...

journal_title：Nucleic acids research

authors： Lok GT,Sy SM,Dong SS,Ching YP,Tsao SW,Thomson TM,Huen MS

更新日期：2012-01-01 00:00:00

abstract：:Spirochetes of the genus Borrelia possess unusual genomes that consist in a linear chromosome and multiple linear and circular plasmids. The linear replicons are terminated by covalently closed hairpin ends, referred to as hairpin telomeres. The hairpin telomeres represent a simple solution to the end-replication prob...

journal_title：Nucleic acids research

authors： Huang SH,Kobryn K

更新日期：2016-06-20 00:00:00

abstract：:Human transcription factor Sp1 contains three contiguous repeats of the C2H2-type zinc finger motif and binds to the decanucleotide sequence 5'-(G/T)GGGCGG(G/A)(G/A)(C/T)-3' (GC box). In order to determine whether the three-zinc finger peptide Sp1(530-623) has selectivity for sequence outside the GC box, we used a sel...

journal_title：Nucleic acids research

authors： Nagaoka M,Shiraishi Y,Sugiura Y

更新日期：2001-12-15 00:00:00

abstract：:Biological evolution resulted in a homochiral world in which nucleic acids consist exclusively of d-nucleotides and proteins made by ribosomal translation of l-amino acids. From the perspective of synthetic biology, however, particularly anabolic enzymes that could build the mirror-image counterparts of biological mac...

journal_title：Nucleic acids research

authors： Pech A,Achenbach J,Jahnz M,Schülzchen S,Jarosch F,Bordusa F,Klussmann S

更新日期：2017-04-20 00:00:00

abstract：:Poly(ADP-ribosyl)ation is involved in numerous bio-logical processes including DNA repair, transcription and cell death. Cellular levels of poly(ADP-ribose) (PAR) are regulated by PAR polymerases (PARPs) and the degrading enzyme PAR glycohydrolase (PARG), controlling the cell fate decision between life and death in re...

journal_title：Nucleic acids research

authors： Illuzzi G,Fouquerel E,Amé JC,Noll A,Rehmet K,Nasheuer HP,Dantzer F,Schreiber V

更新日期：2014-07-01 00:00:00

abstract：:Aberrant DNA methylation at CpG dinucleotides can result in epigenetic silencing of tumour suppressor genes and represents one of the earliest events in tumourigenesis. To date, however, high-throughput tools that are capable of surveying the methylation status of multiple gene promoters have been restricted to a limi...

journal_title：Nucleic acids research

authors： Mund C,Beier V,Bewerunge P,Dahms M,Lyko F,Hoheisel JD

更新日期：2005-04-28 00:00:00

abstract：:Ribosomal RNA (rRNA) in intact ribosomes is cleaved into discrete products on incubation of reticulocyte lysates or L-cell extracts with ppp(A2'p)3A. Cleavage of rRNA may, therefore, provide a useful assay for 2-5A (ppp)A2'p)nA; n = 2 to 4) or for the presence of a 2-5A-dependent nuclease. The results with reticulocyt...

journal_title：Nucleic acids research

authors： Wreschner DH,James TC,Silverman RH,Kerr IM

更新日期：1981-04-10 00:00:00

abstract：:The B-subunits associated with the replicative DNA polymerases are conserved from Archaea to humans, whereas the corresponding catalytic subunits are not related. The latter belong to the B and D DNA polymerase families in eukaryotes and archaea, respectively. Sequence analysis places the B-subunits within the calcine...

journal_title：Nucleic acids research

authors： Jokela M,Eskelinen A,Pospiech H,Rouvinen J,Syväoja JE

更新日期：2004-04-30 00:00:00

abstract：:Chromatin fragments released from intact Friend erythroleukemia cell nuclei during limited incubation with micrococcal nuclease, DNase II or DNase I were analyzed to determine the distribution of DNA methyltransferase in chromatin. The enzyme was released in a free form when internucleosomal DNA was digested with micr...

journal_title：Nucleic acids research

authors： Creusot F,Christman JK

更新日期：1981-10-24 00:00:00

abstract：:Recent studies with poly(ADP-ribose) polymerase (PARP)-deficient mice have highlighted the role of this enzyme in genomic stability and response to various genomic insults. In the absence of DNA damaging treatment, we report here that a PARP-deficient cell line (PARP-/-) established from knockout mice displays a decre...

journal_title：Nucleic acids research

authors： Canitrot Y,de Murcia G,Salles B

更新日期：1998-11-15 00:00:00

abstract：:FIMM database (http://sdmc.krdl.org.sg:8080/fimm) contains data relevant to functional molecular immunology, focusing on cellular immunology. It contains fully referenced data on protein antigens, major histocompatibility complex (MHC) molecules, MHC-associated peptides and relevant disease associations. FIMM has a se...

journal_title：Nucleic acids research

authors： Schönbach C,Koh JL,Flower DR,Wong L,Brusic V

更新日期：2002-01-01 00:00:00