Abstract:
:Mouse DNA contains two equally abundant, homologous subfamilies of MspI 3.6 and 5 kb repeated fragments. The first subfamily corresponds to the previously described (1) Bam 4 kb repeats, the second one to Bam repeated fragments of higher molecular weight. These subfamilies account for the vast majority of long Bam repeats and are linked with contiguous short Bam 0.5 kb repeats. A minority of these composite Bam repeats extend, on the 0.5 kb side, into R repeats. In turn, a fraction of the composite Bam/R repeats extend further, for at least 3 kb, into other repeated sequences contiguous to the R repeats. The long Bam repeats belong, therefore, in at least three superfamilies of repeats, the longest one being over 9 kb in size. Some general properties of these superfamilies are discussed.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Meunier-Rotival M,Bernardi Gdoi
10.1093/nar/12.3.1593subject
Has Abstractpub_date
1984-02-10 00:00:00pages
1593-608issue
3eissn
0305-1048issn
1362-4962journal_volume
12pub_type
杂志文章abstract::Synthesis and radioiodination of a stannyl oligodeoxyribonucleotide were undertaken to evaluate a gamma ray emitting ODN ligand for thrombus imaging in vivo . Synthesis of the ODN was based on modified automatedbeta-cyanoethyl phosphoramidite chemistry with an organotin nucleoside (dU*) coupled to a thrombin binding a...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/25.14.2897
更新日期:1997-07-15 00:00:00
abstract::DrugCentral (http://drugcentral.org) is an open-access online drug compendium. DrugCentral integrates structure, bioactivity, regulatory, pharmacologic actions and indications for active pharmaceutical ingredients approved by FDA and other regulatory agencies. Monitoring of regulatory agencies for new drugs approvals ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw993
更新日期:2017-01-04 00:00:00
abstract::An oligonucleotide hybrid is described which possesses two triple helix forming oligonucleotides which have been connected by a flexible polymeric linker chain. As a prototype, binding of this class of oligonucleotide to duplex DNA has been studied using a segment of the HSV-1 D-glycoprotein promoter, which possesses ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/21.20.4810
更新日期:1993-10-11 00:00:00
abstract::To enhance our knowledge regarding biological pathway regulation, we took an integrated approach, using the biomedical literature, ontologies, network analyses and experimental investigation to infer novel genes that could modulate biological pathways. We first constructed a novel gene network via a pairwise compariso...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gku678
更新日期:2014-10-01 00:00:00
abstract::The covalent attachment of thiol-modified DNA oligomers; to self-assembled monolayer silane films on fused silica and oxidized silicon substrates is described. A heterobifunctional crosslinking molecule bearing both thiol- and amino-reactive moieties was used to tether a DNA oligomer (modified at its terminus with a t...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/24.15.3031
更新日期:1996-08-01 00:00:00
abstract::The secondary structure of V4, the largest variable area of eukaryotic small subunit ribosomal RNA, was re-examined by comparative analysis of 3253 nucleotide sequences distributed over the animal, plant and fungal kingdoms and a diverse set of protist taxa. An extensive search for compensating base pair substitutions...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/28.23.4698
更新日期:2000-12-01 00:00:00
abstract::Myotonic dystrophy type 1 (DM1) is caused by the expansion of a (CTG).(CAG) repeat in the DMPK gene on chromosome 19q13.3. At least 17 neurological diseases have similar genetic mutations, the expansion of DNA repeats. In most of these disorders, the disease severity is related to the length of the repeat expansion, a...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkh976
更新日期:2004-12-01 00:00:00
abstract::We have cloned and sequenced the lasR gene, which is involved in the transcriptional activation of several pathogenic factors, from Pseudomonas aeruginosa IFO3455 and PA103. These clones were predicted to be an open reading frame of 239 amino acids as reported for the PAO1 strain. There is only a single base change re...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/22.18.3706
更新日期:1994-09-11 00:00:00
abstract::It has been shown that the monomethylated cap structure plays important roles in pre-mRNA splicing and nuclear export of RNA. As a candidate for the factor involved in these nuclear events we have previously purified an 80 kDa nuclear cap binding protein (NCBP) from a HeLa cell nuclear extract and isolated its full-le...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/23.18.3638
更新日期:1995-09-25 00:00:00
abstract::enod40 is a plant gene that participates in the regulation of symbiotic interaction between leguminous plants and bacteria or fungi. Furthermore, it has been suggested to play a general role in non-symbiotic plant development. Although enod40 seems to have multiple functions, being present in many land plants, the mol...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm173
更新日期:2007-01-01 00:00:00
abstract::A 200 bp sequence including a stretch of 54 base pairs of alternating guanosine and adenosine nucleotide residues [(dG-dA)27.(dT-dC)27] was cloned in the simian virus 40 (SV40) genome between the KpnI and HpaII sites. This sequence was discovered earlier as part of a region limiting the amplification of sequences adja...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/16.16.8077
更新日期:1988-08-25 00:00:00
abstract::Sequence heterogeneity is a common characteristic of RNA viruses that is often referred to as sub-populations or quasispecies. Traditional techniques used for assembly of short sequence reads produced by deep sequencing, such as de-novo assemblers, ignore the underlying diversity. Here, we introduce a novel algorithm ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx755
更新日期:2017-11-02 00:00:00
abstract::To achieve drug resistance Leishmania parasite alters gene copy number by using its repeated sequences widely distributed through the genome. Even though homologous recombination (HR) is ascribed to maintain genome stability, this eukaryote exploits this potent mechanism driven by the Rad51 recombinase to form benefic...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv118
更新日期:2015-03-11 00:00:00
abstract::The Diatom Expressed Sequence Tag (EST) Database was constructed to provide integral access to ESTs from these ecologically and evolutionarily interesting microalgae. It has now been updated with 130,000 Phaeodactylum tricornutum ESTs from 16 cDNA libraries and 77,000 Thalassiosira pseudonana ESTs from seven libraries...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkn905
更新日期:2009-01-01 00:00:00
abstract::In mammalian cells, DNA damage induces robust changes in gene expression and these changes contribute to the proper execution of cellular responses to DNA damage, including DNA repair, cell cycle arrest and apoptosis. The transcription factor E2F-1 has been suggested to play a key role in the regulation of cell cycle-...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/27.2.491
更新日期:1999-01-15 00:00:00
abstract::Escherichia coli has long been regarded as a model organism in the study of codon usage bias (CUB). However, most studies in this organism regarding this topic have been computational or, when experimental, restricted to small datasets; particularly poor attention has been given to genes with low CUB. In this work, co...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg897
更新日期:2003-12-01 00:00:00
abstract::In Trypanosoma brucei, the mutually exclusive expression of the major surface antigens, the variant surface glycoprotein (VSG) of the bloodstream form and procyclin of the procyclic form, is due to a stage-specific accumulation of the respective mRNAs. Through the targeting of a reporter construct in the procyclin pro...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/23.11.1862
更新日期:1995-06-11 00:00:00
abstract::Antigenic variation of the pilus is an essential component of Neisseria gonorrhoeae pathogenesis. Unidirectional recombination of silent pilin DNA into an expressed pilin gene allows for substantial sequence variation of this highly immunogenic surface structure. While the RecA protein is required for pilin gene recom...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/25.7.1362
更新日期:1997-04-01 00:00:00
abstract::We have studied the effects of the nucleotide analogue, 2',3'-dideoxythymidine-5'-triphosphate (ddTTP) on replicative DNA synthesis in HeLa cell lysates. As previously demonstrated (1), such lysates carry out extensive DNA synthesis in vitro, at rates and in a fashion similar to in vivo DNA replication. We report here...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/5.6.1933
更新日期:1978-06-01 00:00:00
abstract::The inherent properties of DNA as a stable polymer with unique affinity for partner molecules determined by the specific Watson-Crick base pairing makes it an ideal component in self-assembling structures. This has been exploited for decades in the design of a variety of artificial substrates for investigations of DNA...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm1124
更新日期:2008-03-01 00:00:00
abstract::In eukaryotic cells, the shortening and removal of the poly(A) tail of cytoplasmic mRNA by deadenylase enzymes is a critical step in post-transcriptional gene regulation. The ribonuclease activity of deadenylase enzymes is attributed to either a DEDD (Asp-Glu-Asp-Asp) or an endonuclease-exonuclease-phosphatase domain....
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt972
更新日期:2014-03-01 00:00:00
abstract::Mitochondrial-DNA diseases have no effective treatments. Allotopic expression-synthesis of a wild-type version of the mutated protein in the nuclear-cytosolic compartment and its importation into mitochondria-has been proposed as a gene-therapy approach. Allotopic expression has been successfully demonstrated in yeast...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq769
更新日期:2011-01-01 00:00:00
abstract::Phosphorylation of the cellular single-stranded DNA-binding protein, replication protein A (RPA), occurs during normal mitotic cell cycle progression and also in response to genotoxic stress. In budding yeast, these reactions require the ATM homolog Mec1, a central regulator of the DNA replication and DNA damage check...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/29.23.4808
更新日期:2001-12-01 00:00:00
abstract::Interaction detection methods have led to the discovery of thousands of interactions between proteins, and discerning relevance within large-scale data sets is important to present-day biology. Here, a spectral method derived from graph theory was introduced to uncover hidden topological structures (i.e. quasi-cliques...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg340
更新日期:2003-05-01 00:00:00
abstract::Transcription termination defines accurate transcript 3'-ends and ensures programmed transcriptomes, making it critical to life. However, transcription termination mechanisms remain largely unknown in Archaea. Here, we reported the physiological significance of the newly identified general transcription termination fa...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkaa702
更新日期:2020-09-25 00:00:00
abstract::A new system, ZCURVE 1.0, for finding protein- coding genes in bacterial and archaeal genomes has been proposed. The current algorithm, which is based on the Z curve representation of the DNA sequences, lays stress on the global statistical features of protein-coding genes by taking the frequencies of bases at three c...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg254
更新日期:2003-03-15 00:00:00
abstract::SINE-VNTR-Alu (SVA) elements are non-autonomous, hominid-specific non-LTR retrotransposons and distinguished by their organization as composite mobile elements. They represent the evolutionarily youngest, currently active family of human non-LTR retrotransposons, and sporadically generate disease-causing insertions. S...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr863
更新日期:2012-02-01 00:00:00
abstract::The mammalian target of rapamycin (mTOR) is a critical regulator of cell growth, integrating multiple signalling cues and pathways. Key among the downstream activities of mTOR is the control of the protein synthesis machinery. This is achieved, in part, via the co-ordinated regulation of mRNAs that contain a terminal ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkaa1189
更新日期:2021-01-11 00:00:00
abstract::SLM2 and Sam68 are splicing regulator paralogs that usually overlap in function, yet only SLM2 and not Sam68 controls the Neurexin2 AS4 exon important for brain function. Herein we find that SLM2 and Sam68 similarly bind to Neurexin2 pre-mRNA, both within the mouse cortex and in vitro. Protein domain-swap experiments ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw1277
更新日期:2017-04-20 00:00:00
abstract::Biological evolution resulted in a homochiral world in which nucleic acids consist exclusively of d-nucleotides and proteins made by ribosomal translation of l-amino acids. From the perspective of synthetic biology, however, particularly anabolic enzymes that could build the mirror-image counterparts of biological mac...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx079
更新日期:2017-04-20 00:00:00