Abstract:
:It has been shown that the monomethylated cap structure plays important roles in pre-mRNA splicing and nuclear export of RNA. As a candidate for the factor involved in these nuclear events we have previously purified an 80 kDa nuclear cap binding protein (NCBP) from a HeLa cell nuclear extract and isolated its full-length cDNA. In this report, in order to obtain a clue to the cellular functions of NCBP, we attempted to identify a factor(s) that interacts with NCBP. Using the yeast two-hybrid system we isolated three clones from a HeLa cell cDNA library. We designated the proteins encoded by these clones NIPs (NCBP interacting proteins). NIP1 and NIP2 have an RNP consensus-type RNA binding domain, whereas NIP3 contains a unique domain of Arg-Glu or Lys-Glu dipeptide repeats. We also show that NCBP requires NIP1 for binding to the cap structure. Possible roles of NIPs in cap-dependent nuclear processes are discussed.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Kataoka N,Ohno M,Moda I,Shimura Ydoi
10.1093/nar/23.18.3638subject
Has Abstractpub_date
1995-09-25 00:00:00pages
3638-41issue
18eissn
0305-1048issn
1362-4962pii
5b0164journal_volume
23pub_type
杂志文章abstract::By screening a rabbit reticulocyte library, an alternative 15-LOX transcript of 3.6 kb (15-LOX mRNA2) was detected containing a 1019 nt longer 3'-untranslated region (UTR2) than the main 2.6 kb mRNA (15-LOX mRNA1). In anaemic animals, northern blotting showed that 15-LOX mRNA2 was predominantly expressed in non-erythr...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/27.8.1828
更新日期:1999-04-15 00:00:00
abstract::The aspartate protease of Mucor pusillus (Mucor pusillus rennin; MPR) is a milk-clotting enzyme used in the cheese industry. The partial amino acid sequence of MPR was determined and oligonucleotide probes were synthesized for cloning of the MPR gene. A clone giving positive hybridization with the probes was selected ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/14.19.7557
更新日期:1986-10-10 00:00:00
abstract::The study of pathogenic mitochondrial DNA mutations has, in most cases, relied on the production of transmitochondrial cybrids. Although the procedure to produce such cybrids is well established, it is laborious and cumbersome. Moreover, the mechanical enucleation procedure is inefficient and different techniques have...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gng100
更新日期:2003-08-15 00:00:00
abstract::Human U6 small nuclear RNA (U6 snRNA), an abundant snRNA required for splicing of pre-mRNAs, contains several post-transcriptional modifications including a single m6A (N-6-methyladenosine) at position 43. This A-43 residue is critical for the function of U6 snRNA in splicing of pre-mRNAs. Yeast and plant U6 snRNAs al...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/23.13.2421
更新日期:1995-07-11 00:00:00
abstract::Homing endonucleases (HEs) cut long DNA target sites with high specificity to initiate and target the lateral transfer of mobile introns or inteins. This high site specificity of HEs makes them attractive reagents for gene targeting to promote DNA modification or repair. We have generated several hundred catalytically...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp004
更新日期:2009-04-01 00:00:00
abstract::The pol gene of all retroviruses is expressed as a gag-pol fusion protein which is proteolytically processed to produce all viral enzymes. In the human immunodeficiency virus (HIV), the gag and pol genes overlap by 241 nucleotides with pol in the -1 phase with respect to gag. The gag-pol fusion is produced via a -1 ri...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/20.15.3945
更新日期:1992-08-11 00:00:00
abstract::We demonstrate compaction of DNA with nanoscale biomimetic constructs which are robust synthetic analogs of globular proteins. These constructs are approximately 15 nm in diameter, shell crosslinked knedel-like (SCKs) nanoparticles, which are prepared by covalent stabilization of amphiphilic di-block co-polymer micell...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/27.14.2966
更新日期:1999-07-15 00:00:00
abstract::Long Interspersed Element-1 (LINE-1) retrotransposition contributes to inter- and intra-individual genetic variation and occasionally can lead to human genetic disorders. Various strategies have been developed to identify human-specific LINE-1 (L1Hs) insertions from short-read whole genome sequencing (WGS) data; howev...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkz1173
更新日期:2020-02-20 00:00:00
abstract::TcruziDB (http://TcruziDB.org) is an integrated genome database for the parasitic organism Trypanosoma cruzi, the causative agent of Chagas' disease. The database currently incorporates all available sequence data (Genomic, BAC, EST) in a single user-friendly location. The database contains a variety of tools specific...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkh049
更新日期:2004-01-01 00:00:00
abstract::We present the LGA (Local-Global Alignment) method, designed to facilitate the comparison of protein structures or fragments of protein structures in sequence dependent and sequence independent modes. The LGA structure alignment program is available as an online service at http://PredictionCenter.llnl.gov/local/lga. D...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg571
更新日期:2003-07-01 00:00:00
abstract::THY Pol-2 intein, from Thermococcus hydrothermalis, belongs to the same allelic family as TLI Pol-2 (PI-TLII), Tfu Pol-2 (PI-TFUII) and TspTY Pol-3 mini-intein, all inserted at the pol-c site of archaeal DNA polymerase genes. This new intein was cloned, expressed in Escherichia coli and purified. The intein is a speci...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/28.21.4391
更新日期:2000-11-01 00:00:00
abstract::The Galaxy HiCExplorer provides a web service at https://hicexplorer.usegalaxy.eu. It enables the integrative analysis of chromosome conformation by providing tools and computational resources to pre-process, analyse and visualize Hi-C, Capture Hi-C (cHi-C) and single-cell Hi-C (scHi-C) data. Since the last publicatio...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkaa220
更新日期:2020-07-02 00:00:00
abstract::RNA levels in a cell are regulated by the relative rates of RNA synthesis and decay. We recently developed a new approach for measuring both RNA synthesis and decay in a single experimental setting by biosynthetic labeling of newly transcribed RNA. Here, we show that this provides measurements of RNA half-lives from m...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp542
更新日期:2009-09-01 00:00:00
abstract::A direct correlation exists between the level of histone H4 hyperacetylation induced by sodium butyrate and the extent to which nucleosomes lose their compact shape and become elongated (62.0% of the particles have a length/width ratio over 1.6; overall mean in the length/width ratio = 1.83 +/- 0.48) when bound to ele...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/18.9.2739
更新日期:1990-05-11 00:00:00
abstract::Several mono-, di-, tetra-, penta- and nonaribozymes were developed. These multitarget-ribozymes were targeted to cleave HIV-1 env RNA at up to nine different conserved sites. Each multitarget-ribozyme consisted of a chain of up to nine hammerhead motifs, each flanked by a different targeting sequence. The multitarget...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/20.17.4581
更新日期:1992-09-11 00:00:00
abstract::Bloom's syndrome helicase (BLM) is a member of the RecQ family of DNA helicases, which play key roles in the maintenance of genome integrity in all organism groups. We describe crystal structures of the BLM helicase domain in complex with DNA and with an antibody fragment, as well as SAXS and domain association studie...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv373
更新日期:2015-05-26 00:00:00
abstract::Computer programs, implemented on an Univac II00/80 computer system, for the identification and characterization of protein coding genes and for the analysis of nucleic acid sequences, are described. ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/12.1part1.281
更新日期:1984-01-11 00:00:00
abstract::Transfection of a human estrogen receptor cDNA expression vector (HEO) into cultured Xenopus kidney cells confers estrogen responsiveness to the recipient cells as demonstrated by the hormone dependent expression of co-transfected Xenopus vitellogenin-CAT chimeric genes. The estrogen stimulation of these vit-CAT genes...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/16.17.8291
更新日期:1988-09-12 00:00:00
abstract::The transcriptional shift from repression to activation of target genes is crucial for the fidelity of Notch responses through incompletely understood mechanisms that likely involve chromatin-based control. To activate silenced genes, repressive chromatin marks are removed and active marks must be acquired. Histone H3...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw105
更新日期:2016-06-02 00:00:00
abstract::A new thermodynamic database for normal and modified nucleic acids has been developed. This Thermodynamic Database for Nucleic Acids (NTDB) includes sequence, structure and thermodynamic information as well as experimental methods and conditions. In this release, there are 1851 sequences containing both normal and mod...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/29.1.230
更新日期:2001-01-01 00:00:00
abstract::Clustered regularly interspaced short palindromic repeats (CRISPRs) and their associated Cas proteins comprise a prokaryotic RNA-guided adaptive immune system that interferes with mobile genetic elements, such as plasmids and phages. The type I-E CRISPR interference complex Cascade from Escherichia coli is composed of...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gku1285
更新日期:2015-01-01 00:00:00
abstract::The binding of anti poly(I). poly (C) Fab fragments to double or triple stranded polynucletides has been studied by fluorescence. Association constants were deduced from competition experiments. The comparison of the association constants leads to the conclusion that several atoms of the base residues do not interact ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/3.12.3337
更新日期:1976-12-01 00:00:00
abstract::Signal Transducers and Activators of Transcription (STAT) 5A/B regulate cytokine-inducible genes upon binding to GAS motifs. It is not known what percentage of genes with GAS motifs bind to and are regulated by STAT5. Moreover, it is not clear whether genome-wide STAT5 binding is modulated by its concentration. To cla...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks056
更新日期:2012-05-01 00:00:00
abstract::Single-nucleotide variation and gene expression of disease samples represent important resources for biomarker discovery. Many databases have been built to host and make available such data to the community, but these databases are frequently limited in scope and/or content. BioMuta, a database of cancer-associated si...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx907
更新日期:2018-01-04 00:00:00
abstract::Essential genes are genes that are indispensable to support cellular life. These genes constitute a minimal gene set required for a living cell. We have constructed a Database of Essential Genes (DEG), which contains all the essential genes that are currently available. The functions encoded by essential genes are con...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkh024
更新日期:2004-01-01 00:00:00
abstract::The Ensembl project has been aggregating, processing, integrating and redistributing genomic datasets since the initial releases of the draft human genome, with the aim of accelerating genomics research through rapid open distribution of public data. Large amounts of raw data are thus transformed into knowledge, which...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx1098
更新日期:2018-01-04 00:00:00
abstract::CRISPR-Cas (clustered regularly interspaced short palindromic repeats-CRISPR associated) systems allow bacteria to adapt to infection by acquiring 'spacer' sequences from invader DNA into genomic CRISPR loci. Cas proteins use RNAs derived from these loci to target cognate sequences for destruction through CRISPR inter...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv1259
更新日期:2015-12-15 00:00:00
abstract::We identify a new subgroup of Type I Restriction-Modification enzymes that modify cytosine in one DNA strand and adenine in the opposite strand for host protection. Recognition specificity has been determined for ten systems using SMRT sequencing and each recognizes a novel DNA sequence motif. Previously characterized...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw743
更新日期:2016-11-02 00:00:00
abstract::We have studied a novel class of DNA sequences that cause DNA polymerases to pause. These sequences have the central consensus Py-G-C and are not necessarily adjacent to hairpins in the DNA template. Since most consensus sequences do not cause pauses under standard conditions, additional template features must exist t...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/24.14.2774
更新日期:1996-07-15 00:00:00
abstract::Ribosome assembly is a process fundamental for all cellular activities. The efficiency and accuracy of the subunit assembly are tightly regulated and closely monitored. In the present work, we characterized, both compositionally and structurally, a set of in vivo 50S subunit precursors (45S), isolated from a mutant ba...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt423
更新日期:2013-08-01 00:00:00