Abstract:
:Homing endonucleases (HEs) cut long DNA target sites with high specificity to initiate and target the lateral transfer of mobile introns or inteins. This high site specificity of HEs makes them attractive reagents for gene targeting to promote DNA modification or repair. We have generated several hundred catalytically active, monomerized versions of the well-characterized homodimeric I-CreI and I-MsoI LAGLIDADG family homing endonuclease (LHE) proteins. Representative monomerized I-CreI and I-MsoI proteins (collectively termed mCreIs or mMsoIs) were characterized in detail by using a combination of biochemical, biophysical and structural approaches. We also demonstrated that both mCreI and mMsoI proteins can promote cleavage-dependent recombination in human cells. The use of single chain LHEs should simplify gene modification and targeting by requiring the expression of a single small protein in cells, rather than the coordinate expression of two separate protein coding genes as is required when using engineered heterodimeric zinc finger or homing endonuclease proteins.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Li H,Pellenz S,Ulge U,Stoddard BL,Monnat RJ Jrdoi
10.1093/nar/gkp004subject
Has Abstractpub_date
2009-04-01 00:00:00pages
1650-62issue
5eissn
0305-1048issn
1362-4962pii
gkp004journal_volume
37pub_type
杂志文章abstract::Although many tools have been developed to analyze small RNA sequencing (sRNA-Seq) data, it remains challenging to accurately analyze the small RNA population, mainly due to multiple sequence ID assignment caused by short read length. Additional issues in small RNA analysis include low consistency of microRNA (miRNA) ...
journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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更新日期:2002-01-15 00:00:00
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journal_title:Nucleic acids research
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abstract::Molecular Biology Database List (MBDL) includes brief descriptions and pointers to Web sites for the various databases described in this issue as well as other Web sites presenting data sets relevant to molecular biology. This information is compiled into a list (http://www.oup.co.uk/nar/Volume_27/Issue_01/summary/ gk...
journal_title:Nucleic acids research
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abstract::Herein, we describe a case study into the population dynamics of in vitro selection, using RNA-cleaving DNAzymes as a model system. We sought to understand how the composition of the population can change over time in response to different levels of selection pressure, and how well these changes are correlated with se...
journal_title:Nucleic acids research
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2003-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/nar/3.6.1473
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1994-04-25 00:00:00
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abstract::Neph et al. (2012) (Circuitry and dynamics of human transcription factor regulatory networks. Cell, 150: 1274-1286) reported the transcription factor (TF) regulatory networks of 41 human cell types using the DNaseI footprinting technique. This provides a valuable resource for uncovering regulation principles in differ...
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv1095
更新日期:2015-12-02 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/6.11.3581
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journal_title:Nucleic acids research
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doi:10.1093/nar/10.4.1335
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更新日期:1981-11-11 00:00:00
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journal_title:Nucleic acids research
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更新日期:2016-10-14 00:00:00
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journal_title:Nucleic acids research
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doi:10.1093/nar/10.12.3681
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journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/23.9.1625
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1993-05-11 00:00:00