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Analysis of protein binding to the Sma/Cla DNA repeat in pathogenic Neisseriae.

Abstract：

:Antigenic variation of the pilus is an essential component of Neisseria gonorrhoeae pathogenesis. Unidirectional recombination of silent pilin DNA into an expressed pilin gene allows for substantial sequence variation of this highly immunogenic surface structure. While the RecA protein is required for pilin gene recombination, the factors which maintain the silent reservoir of pilin sequences and/or allow unidirectional recombination from silent to expression loci remain undefined. We have previously shown that a conserved sequence at the 3'end of all pilin loci (the Sma/Cla repeat) is required to be present at the expression locus for efficient recombination from the silent loci. In this study, the binding of gonococcal proteins to this DNA sequence was investigated. Gel mobility shift assays and competition experiments using deletion derivatives of the repeat, show that multiple activities bind to different regions of the Sma/Cla repeat and define the boundaries of the binding sequences. Moreover, only the pathogenic Neisseria harbor proteins which specifically bind to this repeat, suggesting a correlation between the expression of these DNA binding proteins and the potential to cause disease.

journal_name

Nucleic Acids Res

journal_title

Nucleic acids research

authors

Wainwright LA,Frangipane JV,Seifert HS

doi

10.1093/nar/25.7.1362

subject

Has Abstract

pub_date

1997-04-01 00:00:00

pages

1362-8

issue

7

eissn

0305-1048

issn

1362-4962

pii

gka251

journal_volume

25

pub_type

杂志文章

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