Abstract:
:mRNA differential display RT-PCR has been extensively used for the isolation of genes differentially expressed between RNA populations. We have assessed its utility for the identification of developmentally regulated genes in plasmid cDNA libraries derived from individual tissues dissected from early mouse embryos. Using plasmid Southern blot hybridisation as a secondary screen, we are able to identify such genes and show by whole-mount in situ hybridisation that their expression pattern is that expected from the differential display profile.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Gupta R,Thomas P,Beddington RS,Rigby PWdoi
10.1093/nar/26.19.4538subject
Has Abstractpub_date
1998-10-01 00:00:00pages
4538-9issue
19eissn
0305-1048issn
1362-4962pii
gkb711journal_volume
26pub_type
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journal_title:Nucleic acids research
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更新日期:2010-01-01 00:00:00
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abstract::Major efforts are underway to elucidate the spatial distribution of ribosomal RNAs (rRNAs) in the ribosome. An especially informative approach is the identification of likely base-base tertiary interactions within the RNA by phylogenetic comparison. Herein evidence is presented for three heretofore unrecognized candid...
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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