Abstract:
:PubChem (https://pubchem.ncbi.nlm.nih.gov) is a popular chemical information resource that serves the scientific community as well as the general public, with millions of unique users per month. In the past two years, PubChem made substantial improvements. Data from more than 100 new data sources were added to PubChem, including chemical-literature links from Thieme Chemistry, chemical and physical property links from SpringerMaterials, and patent links from the World Intellectual Properties Organization (WIPO). PubChem's homepage and individual record pages were updated to help users find desired information faster. This update involved a data model change for the data objects used by these pages as well as by programmatic users. Several new services were introduced, including the PubChem Periodic Table and Element pages, Pathway pages, and Knowledge panels. Additionally, in response to the coronavirus disease 2019 (COVID-19) outbreak, PubChem created a special data collection that contains PubChem data related to COVID-19 and the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Kim S,Chen J,Cheng T,Gindulyte A,He J,He S,Li Q,Shoemaker BA,Thiessen PA,Yu B,Zaslavsky L,Zhang J,Bolton EEdoi
10.1093/nar/gkaa971subject
Has Abstractpub_date
2021-01-08 00:00:00pages
D1388-D1395issue
D1eissn
0305-1048issn
1362-4962pii
5957164journal_volume
49pub_type
杂志文章abstract::We present a new publicly accessible web-service, RiboSoft, which implements a comprehensive hammerhead ribozyme design procedure. It accepts as input a target sequence (and some design parameters) then generates a set of ranked hammerhead ribozymes, which target the input sequence. This paper describes the implemente...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv1111
更新日期:2016-02-29 00:00:00
abstract::The Intronerator (http://www.cse.ucsc.edu/ approximately kent/intronerator/ ) is a set of web-based tools for exploring RNA splicing and gene structure in Caenorhabditis elegans. It includes a display of cDNA alignments with the genomic sequence, a catalog of alternatively spliced genes and a database of introns. The ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/28.1.91
更新日期:2000-01-01 00:00:00
abstract::The Kluyveromyces lactis gene encoding the general transcription factor IIB (TFIIB) was isolated from a genomic library by complementation of the cold-sensitive phenotype conferred by a mutation in the SUA7 gene, which encodes TFIIB in Saccharomyces cerevisiae. DNA sequence analysis of the KI-SUA7 gene revealed a 357 ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/21.15.3413
更新日期:1993-07-25 00:00:00
abstract::The intron 2 of the murine thymidine kinase (TK) gene was observed to contain two DNase hypersensitive site. In vitro footprinting experiments indicated specific binding sites for nuclear proteins which were characterized within the sequence of intron 2. Two GC boxes (binding sites for transcription factor SP1) and tw...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/19.24.6805
更新日期:1991-12-25 00:00:00
abstract::Little is known about the regulation of the DNA damage-mediated gene expression in archaea. Here we report that the addition of actinomycin D to Sulfolobus solfataricus cultures triggers the expression of the radA paralogue sso0777. Furthermore, a specific retarded band is observed when electrophoretic mobility shift ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm782
更新日期:2007-01-01 00:00:00
abstract::The nucleoside compositions of chloroplast and cytoplasmic tRNAPhe's from Euglena gracilis have been determined. The modified nucleoside compositions of these two tRNAs indicate that tRNAPheChl is more similar to procaryotic (E. coli) tRNAPhe than to either the Euglena cytoplasmic tRNAPhe or other eucaryotic cytoplasm...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/3.2.371
更新日期:1976-02-01 00:00:00
abstract::Findings from clinical and biological studies are often not reproducible when tested in independent cohorts. Due to the testing of a large number of hypotheses and relatively small sample sizes, results from whole-genome expression studies in particular are often not reproducible. Compared to single-study analysis, ge...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw797
更新日期:2017-01-09 00:00:00
abstract::The locus DXS269 (P20) defines a deletion hotspot in the distal part of the Duchenne Muscular Dystrophy gene. We have cloned over 90 kilobase-pairs of genomic DNA from this region in overlapping cosmids. The use of whole cosmids as probes in a competitive DNA hybridization analysis proves a fast and convenient method ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/17.14.5611
更新日期:1989-07-25 00:00:00
abstract::The synthesis of a pentadecamer of the 5'-phosphate of the antiviral nucleoside ribavirin (1'-beta-D-ribofuranosyl-1H-1,2,4-triazole-3-carboxamide) has been achieved. This homoribopolymer is terminated at the 5'-position with an (6-aminohexyl)phosphate group to permit conjugation to a carrier and at the 3'-position by...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/18.5.1099
更新日期:1990-03-11 00:00:00
abstract::SRS (Sequence Retrieval System) is a widely used keyword search engine for querying biological databases. BLAST2 is the most widely used tool to query databases by sequence similarity search. These tools allow users to retrieve sequences by shared keyword or by shared similarity, with many public web servers available...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg535
更新日期:2003-07-01 00:00:00
abstract::Applications of conditional gene expression, whether for therapeutic or basic research purposes, are increasingly requiring mammalian gene control systems that exhibit far tighter control properties. While numerous approaches have been used to improve the widely used Tet-regulatory system, many applications, particula...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkn443
更新日期:2008-09-01 00:00:00
abstract::PAHdb (http://www.mcgill.ca/pahdb ) is a curated relational database (Fig. 1) of nucleotide variation in the human PAH cDNA (GenBank U49897). Among 328 different mutations by state (Fig. 2) the majority are rare mutations causing hyperphenylalaninemia (HPA) (OMIM 261600), the remainder are polymorphic variants without...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/26.1.220
更新日期:1998-01-01 00:00:00
abstract::GAP-43 is a membrane phosphoprotein that is important for the development and plasticity of neural connections. In undifferentiated PC12 pheochromocytoma cells, GAP-43 mRNA degrades rapidly ( t = 5 h), but becomes stable when cells are treated with nerve growth factor. To identify trans- acting factors that may influe...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/25.6.1281
更新日期:1997-03-15 00:00:00
abstract::In order to characterize structure-function relationships, the kinetic behavior of chimeric RNA/DNA ribozyme was compared with that of all RNA ribozyme. Determined kcat values were proven to represent the chemical-cleavage step and not the product-dissociation step. In agreement with the finding by Dahm and Uhlenbeck ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/21.24.5656
更新日期:1993-12-11 00:00:00
abstract::HinP1I recognizes and cleaves the palindromic tetranucleotide sequence G downward arrowCGC in DNA. We report three structures of HinP1I-DNA complexes: in the presence of Ca(2+) (pre-reactive complex), in the absence of metal ion (binary complex) and in the presence of Mg(2+) (post-reactive complex). HinP1I forms a bac...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkj484
更新日期:2006-02-09 00:00:00
abstract::Sets of genes expressed in the same tissue are believed to be under the regulation of a similar set of transcription factors, and can thus be assumed to contain similar structural patterns in their regulatory regions. Here we present a study of the structural patterns in promoters of genes expressed specifically in 26...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp866
更新日期:2010-01-01 00:00:00
abstract::We have prepared cDNA libraries for amoebae and plasmodia of the acellular slime mould, Physarum polycephalum. Differential screening was used to isolate cell-type-specific cDNA clones (in bacteriophage M13) and both libraries yielded approximately 5% of such sequences. The amoebal- and plasmodial-specific clones were...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/15.3.933
更新日期:1987-02-11 00:00:00
abstract::Differential scanning microcalorimetry (DSC), UV absorption and circular dichroism (CD) have been used to study structure and stability of linear (lin), open circular (oc), supercoiled (cd) and relaxed circular duplex (rd) DNA and calf thymus (CT) DNA. Investigations were made in low salt buffer and in the presence of...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/16.24.11737
更新日期:1988-12-23 00:00:00
abstract::Triantennary N-acetyl galactosamine (GalNAc, GN3: ), a high-affinity ligand for the hepatocyte-specific asialoglycoprotein receptor (ASGPR), enhances the potency of second-generation gapmer antisense oligonucleotides (ASOs) 6-10-fold in mouse liver. When combined with next-generation ASO designs comprised of short S-c...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gku531
更新日期:2014-07-01 00:00:00
abstract::The reaction of fluorescamine with primary amino groups of tRNAs was investigated. The reagent was attached under mild conditions to the 3'-end of tRNAPhe-C-C-A(3'NH) from yeast and to the minor nucleoside x in E. coli tRNAArg, tRNALys, tRNAMet, tRNAIle and tRNAPhe. The primary aliphatic amino groups of these tRNAs re...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/5.12.4837
更新日期:1978-12-01 00:00:00
abstract::The genome of the cell is often exposed to DNA damaging agents and therefore requires an intricate well-regulated DNA damage response (DDR) to overcome its deleterious effects. The DDR needs proper regulation for its timely activation, repression, as well as appropriate choice of repair pathway. Studies in Saccharomyc...
journal_title:Nucleic acids research
pub_type: 杂志文章,评审
doi:10.1093/nar/gkw1369
更新日期:2017-03-17 00:00:00
abstract::Ribosome synthesis involves the concomitance of pre-rRNA processing and ribosomal protein assembly. In eukaryotes, this is a complex process that requires the participation of specific sequences and structures within the pre-rRNAs, at least 200 trans-acting factors and the ribosomal proteins. There is little informati...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq260
更新日期:2010-08-01 00:00:00
abstract::The GeneNet system is designed for collection and analysis of the data on gene and metabolic networks, signal transduction pathways and kinetic characteristics of elementary processes. In the past 2 years, the GeneNet structure was considerably improved: (i) the current version of the database is now implemented using...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gki077
更新日期:2005-01-01 00:00:00
abstract::Sequences preceding the minimal promoter play a role in the differential expression of the Xenopus somatic and oocyte-type 5S RNA genes. In this report, the somatic sequences between -32 and +37 are shown to increase transcriptional activity in microinjected embryos, yet have little to no effect in microinjected oocyt...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:
更新日期:1989-11-25 00:00:00
abstract::Regulation of gene expression influences almost all biological processes in an organism; sequence-specific DNA-binding transcription factors are critical to this control. For most genomes, the repertoire of transcription factors is only partially known. Hitherto transcription factor identification has been largely bas...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkj131
更新日期:2006-01-01 00:00:00
abstract::RNA is emerging as a key regulator of a plethora of biological processes. While its study has remained elusive for decades, the recent advent of high-throughput sequencing technologies provided the unique opportunity to develop novel techniques for the study of RNA structure and post-transcriptional modifications. Non...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky486
更新日期:2018-09-19 00:00:00
abstract::An oligodeoxyribonucleotide, d(GCTCACAAT-X-ATTGTGAGC), where X represents a hexaethylene glycol chain, was studied using circular dichroism spectroscopy. Its conformation and conformational stability were compared to those of compounds where X was replaced by four thymines and to the duplex of same sequence without lo...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/18.21.6353
更新日期:1990-11-11 00:00:00
abstract::Melting of DNA in a segment bounded at both ends by regions of greater stability during electrophoresis in denaturing gradient gels show complex properties, not accommodated with standard melting theory. Compact bands of some DNA molecules become anomalously broadened at the retardation level in a denaturing gradient,...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/23.14.2775
更新日期:1995-07-25 00:00:00
abstract::Approximately half of all human transcripts contain at least one upstream translational initiation site that precedes the main coding sequence (CDS) and gives rise to an upstream open reading frame (uORF). We generated uORFdb, publicly available at http://cbdm.mdc-berlin.de/tools/uorfdb, to serve as a comprehensive li...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt952
更新日期:2014-01-01 00:00:00
abstract::Pentatricopeptide repeat (PPR) proteins are helical-repeat proteins that offer a promising scaffold for the engineering of proteins to bind specified RNAs. PPR tracts bind RNA in a modular 1-repeat, 1-nucleotide fashion. An amino acid code specifying the bound nucleotide has been elucidated. However, this code does no...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx1288
更新日期:2018-03-16 00:00:00