Abstract:
:Differential scanning microcalorimetry (DSC), UV absorption and circular dichroism (CD) have been used to study structure and stability of linear (lin), open circular (oc), supercoiled (cd) and relaxed circular duplex (rd) DNA and calf thymus (CT) DNA. Investigations were made in low salt buffer and in the presence of 7.2 M NaClO4. The chaotropic action of perchlorate promotes a reduction of the overall stability of DNA, which permits a direct determination of the transition enthalpies of all four DNA configurations. The stabilities against thermal denaturation have been found to increase in the series lin approximately oc less than cd less than rd. These relative stabilities can be rationalized on the basis of the linkage between supercoiling and secondary structural changes in topologically constrained duplex DNA. On the basis of these studies, a model of the melting process could be suggested that is consistent with the energetic and spectroscopic data.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Thumm W,Seidl A,Hinz HJdoi
10.1093/nar/16.24.11737subject
Has Abstractpub_date
1988-12-23 00:00:00pages
11737-57issue
24eissn
0305-1048issn
1362-4962journal_volume
16pub_type
杂志文章abstract::The E3 ubiquitin ligase HUWE1/Mule/ARF-BP1 plays an important role in integrating/coordinating diverse cellular processes such as DNA damage repair and apoptosis. A previous study has shown that HUWE1 is required for the early step of DNA damage-induced apoptosis, by targeting MCL-1 for proteasomal degradation. Howeve...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv325
更新日期:2015-05-19 00:00:00
abstract::Ribosomal recruitment of cellular mRNAs depends on binding of eIF4F to the mRNA's 5'-terminal 'cap'. The minimal 'cap0' consists of N7-methylguanosine linked to the first nucleotide via a 5'-5' triphosphate (ppp) bridge. Cap0 is further modified by 2'-O-methylation of the next two riboses, yielding 'cap1' (m7GpppNmN) ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt1321
更新日期:2014-03-01 00:00:00
abstract::Overexpression of the flap endonuclease FEN1 has been observed in a variety of cancer types and is a marker for poor prognosis. To better understand the cellular consequences of FEN1 overexpression we utilized a model of its Saccharomyces cerevisiae homolog, RAD27. In this system, we discovered that flap endonuclease ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky313
更新日期:2018-06-20 00:00:00
abstract::The sequence of 110 nucleotides adjacent to the 3'-end of Neurospora crassa 25S-rRNA has been derived by chemical sequencing methods. Sequences present between 40 and 85 nucleotides of the 3'-end were found to complement sequences at the 3'- and 5'-ends of 5.8S-rRNA. Interaction was shown to occur between 5.8S-rRNA an...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/9.5.1111
更新日期:1981-03-11 00:00:00
abstract::EMAGE (http://genex.hgu.mrc.ac.uk/Emage/database) is a database of in situ gene expression patterns in the developing mouse embryo. Domains of expression from raw data images are spatially integrated into a set of standard 3D virtual mouse embryos at different stages of development, allowing data interrogation by spat...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm938
更新日期:2008-01-01 00:00:00
abstract::The chloroplast ribosomal protein CS1 is an essential component of the plastids translational machinery involved in translation initiation. Southern analysis suggests that the corresponding nuclear gene is present in one copy in the spinach genome. We have isolated and sequenced the gene (rps1) to study its expression...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/20.16.4153
更新日期:1992-08-25 00:00:00
abstract::We examined the mechanism regulating the cellular levels of PNKP, the major kinase/phosphatase involved in the repair of oxidative DNA damage, and find that it is controlled by ATM phosphorylation and ubiquitylation-dependent proteasomal degradation. We discovered that ATM-dependent phosphorylation of PNKP at serines ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks909
更新日期:2012-12-01 00:00:00
abstract::HBV genotypes differ in pathogenicity. In addition, genotype-specific differences in the regulation of transcription and virus replication exist in HBV, but the underlying mechanisms are unknown. Here, we show the presence of a G-quadruplex motif in the promoter of the preS2/S gene; this G-quadruplex is highly conserv...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx823
更新日期:2017-11-02 00:00:00
abstract::A protein-DNA complex has less gel electrophoretic mobility than the free DNA fragment. One parameter for the degree of retardation of a linear DNA fragment in a protein-DNA complex is the molecular weight of the bound protein(s). The quotient of the migration distances of free DNA (m) and protein-DNA complex (m') is ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/16.12.5241
更新日期:1988-06-24 00:00:00
abstract::Enhancers are a class of cis-regulatory elements that can increase gene transcription by forming loops in intergenic regions, introns and exons. Enhancers, as well as their associated target genes, and transcription factors (TFs) that bind to them, are highly associated with human disease and biological processes. Alt...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkz973
更新日期:2020-01-08 00:00:00
abstract::Functionality of the non-coding transcripts encoded by the human genome is the coveted goal of the modern genomics research. While commonly relied on the classical methods of forward genetics, integration of different genomics datasets in a global Systems Biology fashion presents a more productive avenue of achieving ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw162
更新日期:2016-04-20 00:00:00
abstract::The transcriptional promoter of satellite 2 from the eastern newt, Notophthalmus viridescens, was analyzed by assaying the activity of deleted or mutated satellite 2 clones in Xenopus laevis oocytes. Two elements in the promoter were found to be important for transcription. These elements have sequences that are simil...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/22.22.4697
更新日期:1994-11-11 00:00:00
abstract::The DNA damage response is vigorously activated by DNA double-strand breaks (DSBs). The chief mobilizer of the DSB response is the ATM protein kinase. We discovered that the COP9 signalosome (CSN) is a crucial player in the DSB response and an ATM target. CSN is a protein complex that regulates the activity of cullin ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv270
更新日期:2015-05-19 00:00:00
abstract::The Blocks Database contains multiple alignments of conserved regions in protein families which can be searched by e-mail (blocks@blocks.fhcrc.org) and World Wide Web (http://blocks.fhcrc.org/ ) servers to classify protein and nucleotide sequences. Recent enhancements to the servers include: (i) improved calculation o...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/25.1.222
更新日期:1997-01-01 00:00:00
abstract::Site-specific mutation was demonstrated in a shuttle vector system using nitrogen mustard-conjugated oligodeoxyribonucleotides (ODNs). Plasmid DNA was modified in vitro by ODNs containing all four DNA bases in the presence of Escherichia coli RecA protein. Up to 50% of plasmid molecules were alkylated in the targeted ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/27.24.e38
更新日期:1999-12-15 00:00:00
abstract::An 11 S ribonucleoprotein particle was isolated from maize endosperm and shown to be functionally and structurally equivalent to the mammalian signal recognition particle. However, unlike animal cells which apparently contain a single 7 SL RNA species, maize endosperm contains a heterogeneous population of 7 SL RNA. T...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/17.4.1573
更新日期:1989-02-25 00:00:00
abstract::The human 7SL RNA component of the signal recognition particle can be separated into four major conformers by nondenaturing polyacrylamide gel electrophoresis. We have investigated what sequences give 7SL RNA the property to exist in different conformations. The human 7SL gene 7L30.1 was mutagenized using the random l...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/14.11.4639
更新日期:1986-06-11 00:00:00
abstract::Controllable gene delivery via vector-based systems remains a formidable challenge in mammalian synthetic biology and a desirable asset in gene therapy applications. Here, we introduce a methodology to control the copies and residence time of a gene product delivered in host human cells but also selectively disrupt fr...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gku1326
更新日期:2015-01-01 00:00:00
abstract::The transcriptional silencing of one of the female X-chromosomes is a finely regulated process that requires accumulation in cis of the long non-coding RNA X-inactive-specific transcript (Xist) followed by a series of epigenetic modifications. Little is known about the molecular machinery regulating initiation and mai...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks968
更新日期:2013-01-07 00:00:00
abstract::Aedes aegypti mosquitoes vector several arboviruses of global health significance, including dengue viruses and chikungunya virus. RNA interference (RNAi) plays an important role in antiviral immunity, gene regulation and protection from transposable elements. Double-stranded RNA binding proteins (dsRBPs) are importan...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv152
更新日期:2015-04-20 00:00:00
abstract::The field bean, Vicia faba L. var. minor, possesses two sub-families of 11 S legumin genes named A and B. We isolated from a genomic library a B-type gene (LeB4) and determined its primary DNA sequence. Gene LeB4 codes for a 484 amino acid residue prepropolypeptide, encompassing a signal peptide of 22 amino acid resid...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/14.6.2707
更新日期:1986-03-25 00:00:00
abstract::The circadian clock comprises transcriptional feedback loops of clock genes. Cryptochromes are essential components of the negative feedback loop in mammals as they inhibit CLOCK-BMAL1-mediated transcription. We purified mouse CRY1 (mCRY1) protein complexes from Sarcoma 180 cells to determine their roles in circadian ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkn1013
更新日期:2009-03-01 00:00:00
abstract::Eukaryotic RNases H from Saccharomyces cerevisiae , Schizosaccharomyces pombe and Crithidia fasciculata , unlike the related Escherichia coli RNase HI, contain a non-RNase H domain with a common motif. Previously we showed that S.cerevisiae RNase H1 binds to duplex RNAs (either RNA-DNA hybrids or double-stranded RNA) ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/26.7.1834
更新日期:1998-04-01 00:00:00
abstract::A small family of DNA sequences is rearranged during the development of the somatic nucleus in Tetrahymena. The family is defined by 266 bp of highly conserved sequence which restriction mapping, hybridization and sequence analysis have shown is shared by a cloned micronuclear fragment and three sequences which consti...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/14.20.8007
更新日期:1986-10-24 00:00:00
abstract::Pax6 transcription is under the control of two main promoters (P0 and P1), and these are autoregulated by Pax6. Additionally, Pax6 expression is under the control of the TGFbeta superfamily, although the precise mechanisms of such regulation are not understood. The effect of TGFbeta on Pax6 expression was studied in t...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl1105
更新日期:2007-01-01 00:00:00
abstract::A newly designed cyclic bis-naphthyridine carbamate dimer CMBL4: with a limited conformational flexibility was synthesized and characterized. Absorption spectra revealed that two naphthyridines in CMBL4: were stacked on each other in aqueous solutions. The most efficient binding of CMBL4: to DNA was observed for the s...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw672
更新日期:2016-09-06 00:00:00
abstract::High-throughput screening assays have been developed to rapidly identify small molecule inhibitors targeting catalytic group I introns. Biochemical reactions catalyzed by a self-splicing group I intron derived from Pneumocystis carinii or from bacteriophage T4 have been investigated. In vitro biochemical assays amenab...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/24.24.5051
更新日期:1996-12-15 00:00:00
abstract::Egr-1 is a murine zinc finger encoding cDNA whose expression is modulated by a variety of ligand-receptor interactions and is often coregulated with c-fos (1). This study reports the isolation of a mouse Egr-1 genomic clone, its intron-exon structure, and 935 bp of 5' flanking sequence. The gene spans about 3.8 kb and...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/16.18.8835
更新日期:1988-09-26 00:00:00
abstract::cDNA cloning is a central technology in molecular biology. cDNA sequences are used to determine mRNA transcript structures, including splice junctions, open reading frames (ORFs) and 5'- and 3'-untranslated regions (UTRs). cDNA clones are valuable reagents for functional studies of genes and proteins. Expressed Sequen...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gni184
更新日期:2005-12-02 00:00:00
abstract::The promoter of the human BCR gene, regulating the transcription of the chimeric BCR/ABL mRNA in leukemia, has been isolated and characterized. A region of 1.1 kb immediately 5' to the transcription start site was analyzed in detail by sequencing, DNase 1 footprinting, gel retardation and functional studies. These exp...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/18.23.7119
更新日期:1990-12-11 00:00:00