Abstract:
:Controllable gene delivery via vector-based systems remains a formidable challenge in mammalian synthetic biology and a desirable asset in gene therapy applications. Here, we introduce a methodology to control the copies and residence time of a gene product delivered in host human cells but also selectively disrupt fragments of the delivery vehicle. A crucial element of the proposed system is the CRISPR protein Cas9. Upon delivery, Cas9 guided by a custom RNA sequence cleaves the delivery vector at strategically placed targets thereby inactivating a co-expressed gene of interest. Importantly, using experiments in human embryonic kidney cells, we show that specific parameters of the system can be adjusted to fine-tune the delivery properties. We envision future applications in complex synthetic biology architectures, gene therapy and trace-free delivery.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Moore R,Spinhirne A,Lai MJ,Preisser S,Li Y,Kang T,Bleris Ldoi
10.1093/nar/gku1326subject
Has Abstractpub_date
2015-01-01 00:00:00pages
1297-303issue
2eissn
0305-1048issn
1362-4962pii
gku1326journal_volume
43pub_type
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