Abstract：
:We have previously mapped a repression domain from the active transcriptional repressor E4BP4 to a 65 amino acid segment near the C-terminus of the polypeptide. Here we show that the E4BP4 repression domain interacts specifically with the TBP binding repressor protein Dr1. Mutants that affect the ability of E4BP4 to bring about transcriptional repression are also deficient in their binding of Dr1. The results are discussed in the light of evidence for squelching of a 'global' repressor by a DNA binding defective E4BP4 mutant.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Cowell IG,Hurst HCdoi
10.1093/nar/24.18.3607subject
Has Abstractpub_date
1996-09-15 00:00:00pages
3607-13issue
18eissn
0305-1048issn
1362-4962pii
6w0069journal_volume
24pub_type
杂志文章abstract：:Bcg I and Bcg I-like restriction endonucleases cleave double stranded DNA specifically on both sides of their asymmetric recognition sequences which are interrupted by several ambiguous base pairs. Their heterosubunit structure, bifunctionality and stimulation by AdoMet make them different from other classified restri...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/25.22.4444
更新日期：1997-11-15 00:00:00
abstract：:Restriction endonucleases HindII and TaqI, but not SalI, were found to efficiently cleave synthetic hexadecanucleotide duplexes which contained either an A/C or a G/T mismatch within their respective restriction sites. Double-stranded M13 DNAs with identical mismatches were also cleaved under the assay conditions. The...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/14.5.1943
更新日期：1986-03-11 00:00:00
abstract：:Bacillus subtilis LrpC is a sequence-independent DNA-binding and DNA-bending protein, which binds both single-stranded (ss) and double-stranded (ds) DNA and facilitates the formation of higher order protein-DNA complexes in vitro. LrpC binds at different sites within the same DNA molecule promoting intramolecular liga...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkj418
更新日期：2006-01-10 00:00:00
abstract：:Novel techniques for high-throughput steady-state metabolomic profiling yield information about changes of nearly thousands of metabolites. Such metabolomic profiles, when analyzed together with transcriptional profiles, can reveal novel insights about underlying biological processes. While a number of conceptual appr...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw266
更新日期：2016-07-08 00:00:00
abstract：:We have sequenced the external transcribed spacer (ETS) of a ribosomal transcription unit from Xenopus laevis, together with sections of the preceding non-transcribed spacer. Our analysis was carried out on the same cloned transcription unit as that from which the internal transcribed spacers (ITS) were previously seq...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/10.7.2387
更新日期：1982-04-10 00:00:00
abstract：:The secondary structures of the 5'-untranslated region (5'-UTR) of five different tymoviruses have been determined by structure probing, computer prediction and sequence comparison. Despite large sequence differences, there are remarkable similarities in the secondary structure. In all viruses two or four hairpins are...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/24.24.4910
更新日期：1996-12-15 00:00:00
abstract：:The molecular structure of the DNA double helix has been known for 60 years, but we remain surprisingly ignorant of the balance of forces that determine its mechanical properties. The DNA double helix is among the stiffest of all biopolymers, but neither theory nor experiment has provided a coherent understanding of t...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt808
更新日期：2013-12-01 00:00:00
abstract：:The cloned 18 S ribosomal RNA gene from Saccharomyces cerevisiae have been sequenced, using the Maxam-Gilbert procedure. From this data the complete sequence of 1789 nucleotides of the 18 S RNA was deduced. Extensive homology with many eucaryotic as well as E. coli ribosomal small subunit rRNA (S-rRNA) has been observ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/8.23.5779
更新日期：1980-12-11 00:00:00
abstract：:Ribosomal protein L4 of Escherichia coli regulates expression of its own eleven gene S10 operon both by inhibiting translation and by stimulating premature termination of transcription. Both regulatory processes presumably involve L4 recognition of the S10 leader RNA. To help define L4's regulatory target, we have inv...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/21.10.2429
更新日期：1993-05-25 00:00:00
abstract：:Post-translational modifications (PTMs) play a key role in numerous cellular processes by directly affecting structure, dynamics and interaction networks of target proteins. Despite their importance, our understanding of protein PTMs at the atomistic level is still largely incomplete. Molecular dynamics (MD) simulatio...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt416
更新日期：2013-07-01 00:00:00
abstract：:The current pandemic situation caused by the Betacoronavirus SARS-CoV-2 (SCoV2) highlights the need for coordinated research to combat COVID-19. A particularly important aspect is the development of medication. In addition to viral proteins, structured RNA elements represent a potent alternative as drug targets. The s...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa1013
更新日期：2020-12-16 00:00:00
abstract：:A solution of benzenesulphonic acid (3%, w/v) in a dimethylformamide and dichloromethane mixture (9:1, v/v) is shown to be a very effective reagent for the detritylation of deoxyoligonucleotides attached to a solid support. The levels of depurination with this reagent were lower than those observed with other reagents...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/10.18.5605
更新日期：1982-09-25 00:00:00
abstract：:A prototype of a novel class of DNA alkylating agents, which combines the DNA crosslinking moiety chlorambucil (Chl) with a sequence-selective hairpin pyrrole-imidazole polyamide ImPy-beta-ImPy-gamma-ImPy-beta-Dp (polyamide 1), was evaluated for its ability to damage DNA and induce biological responses. Polyamide 1-Ch...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkg215
更新日期：2003-02-15 00:00:00
abstract：:The nematode Caenorhabditis elegans contains a small family of vitellogenin genes which is expressed abundantly, but only in the intestine of the adult hermaphrodite worm. In order to identify possible regulatory elements, we have sequenced the DNA surrounding the 5' ends of five of the six genes. Contained within reg...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/13.14.5283
更新日期：1985-07-25 00:00:00
abstract：:A soluble enzyme system that specifically initiates lambda dv plasmid DNA replication at a bacteriophage lambda replication origin [Wold et al. (1982) Proc. Natl. Acad. Sci. USA 79, 6176-6180] is also capable of replicating the single-stranded circular chromosomes of phages M13 and phi X174 to a duplex form. This chai...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/12.7.3069
更新日期：1984-04-11 00:00:00
abstract：:Using a genetic selection for suppressors of a UGA nonsense mutation in trpA, we have isolated a G to A transition mutation at position 1491 in the decoding region of 16S rRNA. This suppressor displayed no codon specificity, suppressing UGA, UAG and UAA nonsense mutations and +1 and -1 frameshift mutations in lacZ. Su...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/23.21.4234
更新日期：1995-11-11 00:00:00
abstract：:Existence of some extra-genetic (epigenetic) codes has been postulated since the discovery of the primary genetic code. Evident effects of histone post-translational modifications or DNA methylation over the efficiency and the regulation of DNA processes are supporting this postulation. EMdeCODE is an original algorit...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks1214
更新日期：2013-02-01 00:00:00
abstract：:Discovering which regulatory proteins, especially transcription factors (TFs), are active under certain experimental conditions and identifying the corresponding binding motifs is essential for understanding the regulatory circuits that control cellular programs. The experimental methods used for this purpose are labo...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp554
更新日期：2009-09-01 00:00:00
abstract：:RNA-binding proteins (RBPs) play important roles in the post-transcriptional control of RNAs. Identifying RBP binding sites and characterizing RBP binding preferences are key steps toward understanding the basic mechanisms of the post-transcriptional gene regulation. Though numerous computational methods have been dev...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkv1025
更新日期：2016-02-29 00:00:00
abstract：:The FREP database (http://facts.gsc.riken.go.jp/FREP/) contains 31 396 RepeatMasker-identified non-redundant variant repeat sequences derived from 16,527 mouse cDNAs with protein-coding potential. The repeats were computationally associated with potential effects on transcriptional variation, translation, protein func...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkh123
更新日期：2004-01-01 00:00:00
abstract：:Single-cell RNA-sequencing (scRNA-seq) enables the characterization of transcriptomic profiles at the single-cell resolution with increasingly high throughput. However, it suffers from many sources of technical noises, including insufficient mRNA molecules that lead to excess false zero values, termed dropouts. Comput...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa506
更新日期：2020-09-04 00:00:00
abstract：:In the battle against viruses, antiviral peptides (AVPs) had demonstrated the immense potential. Presently, more than 15 peptide-based drugs are in various stages of clinical trials. Emerging and re-emerging viruses further emphasize the efforts to accelerate antiviral drug discovery efforts. Despite, huge importance ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks450
更新日期：2012-07-01 00:00:00
abstract：:Three endonuclease activities have been partially purified from Saccharomyces cerevisiae on the basis of their activity against x-irradiated closed-circular supercoiled bacteriophage PM2 DNA. These endonucleases also nick apurinic DNA and two out of the three activities incise DNA UV-irradiated with high doses. The en...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/5.9.3347
更新日期：1978-09-01 00:00:00
abstract：:An enzyme activity introducing an alkali-labile site at 2-hydroxyadenine (2-OH-A) in double-stranded oligonucleotides was detected in nuclear extracts of Jurkat cells. This activity co-eluted with activities toward adenine paired with guanine and 8-oxo-7,8-dihydroguanine (8-oxoG) as a single peak corresponding to a 55...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/28.6.1355
更新日期：2000-03-15 00:00:00
abstract：:Oligodeoxyribonucleotides incorporating non-hydrolyzable dialkyl sulfide linked thymidine dimers (TsT) were synthesized chemically by the solid-phase approach. The sulfide dimer TsT was stable to degradation by snake-venom phosphodiesterase, calf spleen phosphodiesterase, Nuclease P1 and Nuclease S1. Thermal denaturat...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/21.6.1473
更新日期：1993-03-25 00:00:00
abstract：:Type I toxin-antitoxin (TA) systems have been identified in a wide range of bacterial genomes. Here, we report the characterization of a new type I TA system present on the chromosome of the major human gastric pathogen, Helicobacter pylori. We show that the aapA1 gene encodes a 30 amino acid peptide whose artificial ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw1343
更新日期：2017-05-05 00:00:00
abstract：:To investigate molecular controls of cardiomyocyte proliferation, we utilized cardiomyocytes induced to proliferate indefinitely by SV40 large T antigen (T-ag). In the T-ag-immortalized AT-1, AT-2 and HL-1 cardiomyocytes, normal cellular proteins associating with T-ag and p53 were identified, isolated and micro-sequen...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/28.15.2882
更新日期：2000-08-01 00:00:00
abstract：:During the past years, the advances in high-throughput technologies have produced an unprecedented growth in the number and size of repositories and databases storing relevant biological data. Today, there is more biological information than ever but, unfortunately, the current status of many of these repositories is ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks575
更新日期：2012-07-01 00:00:00
abstract：:Over the past 10 years, fluorescent end-labeling of DNA fragments has evolved into the preferred method of DNA detection for a wide variety of applications, including DNA sequencing and PCR fragment analysis. One of the advantages inherent in fluorescent detection methods is the ability to perform multi-color analyses...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/26.11.2797
更新日期：1998-06-01 00:00:00
abstract：:Using a combination of bioinformatic and molecular biology approaches a Drosophila melanogaster protein, DmTTF, has been identified, which exhibits sequence and structural similarity with two mitochondrial transcription termination factors, mTERF (human) and mtDBP (sea urchin). Import/processing assays indicate that D...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkg272
更新日期：2003-03-15 00:00:00
