Abstract:
:A soluble enzyme system that specifically initiates lambda dv plasmid DNA replication at a bacteriophage lambda replication origin [Wold et al. (1982) Proc. Natl. Acad. Sci. USA 79, 6176-6180] is also capable of replicating the single-stranded circular chromosomes of phages M13 and phi X174 to a duplex form. This chain initiation on single-stranded templates is novel in that it is absolutely dependent on the lambda O and P protein chromosomal initiators and on several Escherichia coli proteins that are known to function in the replication of the lambda chromosome in vivo, including the host dnaB, dnaG (primase), dnaJ and dnaK replication proteins. Strand initiation occurs at multiple sites following an O and P protein-dependent pre-priming step in which the DNA is converted into an activated nucleoprotein complex containing the bacterial dnaB protein. We propose a scheme for the initiation of DNA synthesis on single-stranded templates in this enzyme system that may be relevant to strand initiation events that occur during replication of phage lambda in vivo.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
LeBowitz JH,McMacken Rdoi
10.1093/nar/12.7.3069subject
Has Abstractpub_date
1984-04-11 00:00:00pages
3069-88issue
7eissn
0305-1048issn
1362-4962journal_volume
12pub_type
杂志文章abstract::The cDNA encoding the human RNA lariat debranching enzyme (hDBR1) was identified and cloned by searching the Expressed Sequence Tag (EST) database and screening a HeLa cDNA library, based on predicted amino acid sequence homologies with the Saccharomyces cerevisiae, Schizosaccharomyces pombe and Caenorhabditis elegans...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/28.18.3666
更新日期:2000-09-15 00:00:00
abstract::Using a human interferon-alpha probe we have isolated recombinant phages containing murine interferon-alpha (Mu IFN-alpha) genes from a genomic library. One of these phages contained two complete Mu IFN-alpha genes and part of a third gene. The insert of a second phage held two IFN genes. This indicates that the Mu IF...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/13.3.791
更新日期:1985-02-11 00:00:00
abstract::The endoribonuclease RNase E is a key enzyme in RNA metabolism for many bacterial species. In Escherichia coli, RNase E contributes to the majority of RNA turnover and processing events, and the enzyme has been extensively characterized as the central component of the RNA degradosome assembly. A similar RNA degradosom...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gku1134
更新日期:2014-12-01 00:00:00
abstract::MicroRNA 26a (miR-26a) reduces cell viability in several cancers, indicating that miR-26a could be used as a therapeutic option in patients. We demonstrate that miR-26a not only inhibits G1-S cell cycle transition and promotes apoptosis, as previously described, but also regulates multiple cell cycle checkpoints. We s...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx022
更新日期:2017-05-05 00:00:00
abstract::We have determined the nucleotide sequence at the 3' end of the vesicular stomatitis virus (VSV) polymerase or L gene and compared it to that obtained from a defective interfering particle (DI) RNA generated by this virus. The latter (DI 0.50) contains a large internal deletion within this gene. The deletion begins ex...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/10.21.6919
更新日期:1982-11-11 00:00:00
abstract::Termination of protein synthesis is promoted in ribosomes by proper stop codon discrimination by class 1 polypeptide release factors (RFs). A large set of prokaryotic RFs differing in stop codon specificity, RF1 for UAG and UAA, and RF2 for UGA and UAA, was analyzed by means of a recently developed computational metho...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gki841
更新日期:2005-09-14 00:00:00
abstract::The beta-globin gene of a patient with mRNA-deficient beta o-thalassemia has been sequenced. We find a single nucleotide deletion in amino acid codon 44 that produces a UGA terminator at codon 60. We have previously shown that the beta-globin mRNA of this patient is correctly spliced and polyadenylated, but rapidly tu...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/10.18.5421
更新日期:1982-09-25 00:00:00
abstract::We have used paired-end sequencing of yeast nucleosomal DNA to obtain accurate genomic maps of nucleosome positions and occupancies in control cells and cells treated with 3-aminotriazole (3AT), an inducer of the transcriptional activator Gcn4. In control cells, 3AT-inducible genes exhibit a series of distinct nucleos...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr643
更新日期:2011-12-01 00:00:00
abstract::In this paper, we present evidence that an efficient Rho-dependent terminator can be created by introducing a simple (AG/TC) n DNA repeat into a transcription unit. The Rho termination activity in vivo and in vitro is dependent on the length and the orientation of the insert. The transcription of at least 30 bp of the...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/26.21.4895
更新日期:1998-11-01 00:00:00
abstract::Homing endonucleases recognize long target DNA sequences generating an accurate double-strand break that promotes gene targeting through homologous recombination. We have modified the homodimeric I-CreI endonuclease through protein engineering to target a specific DNA sequence within the human RAG1 gene. Mutations in ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq801
更新日期:2011-01-01 00:00:00
abstract::The DEAH-box NTPase Prp43 disassembles spliceosomes in co-operation with the cofactors Ntr1/Spp382 and Ntr2, forming the NTR complex. How Prp43 is regulated by its cofactors to discard selectively only intron-lariat spliceosomes (ILS) and defective spliceosomes and to prevent disassembly of earlier and properly assemb...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw1225
更新日期:2017-04-20 00:00:00
abstract::Translin is a single-stranded RNA- and DNA-binding protein, which has been highly conserved in eukaryotes, from man to Schizosaccharomyces pombe. TRAX is a Translin paralog associated with Translin, which has coevolved with it. We generated structural models of the S. pombe Translin (spTranslin), based on the solved 3...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp1230
更新日期:2010-05-01 00:00:00
abstract::Lambda exonuclease processively degrades one strand of double-stranded DNA (dsDNA) in the 5"-3" direction. To understand the mechanism through which this enzyme generates high processivity we are analyzing the first step in the reaction, namely the interaction of lambda exonuclease with the ends of substrate DNA. Endo...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/27.15.3057
更新日期:1999-08-01 00:00:00
abstract::The Moloney murine leukemia virus (MLV) is a prototype gammaretrovirus requiring nuclear disassembly before DNA integration. In the nucleus, integration site selection towards promoter/enhancer elements is mediated by the host factor bromo- and extraterminal domain (BET) proteins (bromodomain (Brd) proteins 2, 3 and 4...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky1157
更新日期:2019-02-20 00:00:00
abstract::A RNA interference (RNAi) like mechanism is involved in elimination of thousands of DNA segments from the developing somatic macronucleus of Tetrahymena, yet how specific internal eliminated sequences (IESs) are recognized remains to be fully elucidated. To define requirements for DNA rearrangement, we performed mutag...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl699
更新日期:2006-01-01 00:00:00
abstract::The eukaryotic linear motif (ELM) resource is a repository of manually curated experimentally validated short linear motifs (SLiMs). Since the initial release almost 20 years ago, ELM has become an indispensable resource for the molecular biology community for investigating functional regions in many proteins. In this...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkz1030
更新日期:2020-01-08 00:00:00
abstract::Au (III) reacts with DNA at pH 5.6 to form a complex which is sensitive to mid-UV radiation. Cyclobutane pyrimidine dimers are produced at some 15 to 30 times the rate that they are in untreated DNA. The mechanism of photosensitization appears to involve energy absorption by Au-urine and Au-cytosine adducts which can ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/5.10.3731
更新日期:1978-10-01 00:00:00
abstract::We studied fluorescence intensity, polarization and lifetime of some commonly used fluorophores conjugated to oligodeoxyribonucleotides with different primary and secondary structures. We found that fluorescence intensity can increase or decrease upon hybridization of the labeled strand to its complement depending on ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/30.9.2089
更新日期:2002-05-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gku1194
更新日期:2015-01-01 00:00:00
abstract::Ribosome biogenesis is a key process for maintaining protein synthetic capacity in dividing or growing cells, and requires coordinated production of ribosomal proteins and ribosomal RNA (rRNA), including the processing of the latter. Signalling through mammalian target of rapamycin complex 1 (mTORC1) activates all the...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gku130
更新日期:2014-04-01 00:00:00
abstract::The S-adenosylmethionine (AdoMet) analog sinefungin is a natural product antibiotic that inhibits nucleic acid methyltransferases and arrests the growth of unicellular eukarya and eukaryal viruses. The basis for the particular sensitivity of fungi and protozoa to sinefungin is not known. Here we report the isolation a...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm817
更新日期:2007-01-01 00:00:00
abstract::Unwanted side effects of drugs are a burden on patients and a severe impediment in the development of new drugs. At the same time, adverse drug reactions (ADRs) recorded during clinical trials are an important source of human phenotypic data. It is therefore essential to combine data on drugs, targets and side effects...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv1075
更新日期:2016-01-04 00:00:00
abstract::An endo-exonuclease has been purified from cultured monkey (CV-1) cells. The enzyme which was purified to near homogeneity to be a 65 kDa monomeric protein. The single-strand DNase activity is endonucleolytic and nonprocessive, whereas the double-strand DNase activity is exonucleolytic and processive. The enzyme was a...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/20.16.4355
更新日期:1992-08-25 00:00:00
abstract::We have previously described programs for a variety of types of sequence analysis (1-4). These programs have now been integrated into a single package. They are written in the standard C programming language and run on virtually any computer system with a C compiler, such as the IBM/PC and other computers running unde...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/14.1.443
更新日期:1986-01-10 00:00:00
abstract::The thermal stabilities of RNA:DNA hybrids are substantially greater than those of DNA:DNA duplexes in aqueous electrolyte solutions containing high concentrations of formamide. Association rates to form DNA:DNA duplexes and DNA:RNA hybrids have been measured in these solvents. There is a temperature range in which DN...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/4.5.1539
更新日期:1977-01-01 00:00:00
abstract::The Chinese hamster cell line, DC-3F, is heterozygous at the DHFR locus, and each allele can be distinguished on the basis of a unique DNA restriction pattern, protein isoelectric profile and in the abundancy of the DHFR mRNAs it expresses. Although each allele produces four transcripts, 1000, 1650 and 2150 nucleotide...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/20.24.6597
更新日期:1992-12-25 00:00:00
abstract::microRNAs (miRNAs) are small endogenous RNA molecules that are implicated in many biological processes through post-transcriptional regulation of gene expression. The DIANA-microT Web server provides a user-friendly interface for comprehensive computational analysis of miRNA targets in human and mouse. The server has ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr294
更新日期:2011-07-01 00:00:00
abstract::Mouse DNA contains two equally abundant, homologous subfamilies of MspI 3.6 and 5 kb repeated fragments. The first subfamily corresponds to the previously described (1) Bam 4 kb repeats, the second one to Bam repeated fragments of higher molecular weight. These subfamilies account for the vast majority of long Bam rep...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/12.3.1593
更新日期:1984-02-10 00:00:00
abstract::Minigenes encoding the peptide Met-Arg-Arg have been used to study the mechanism of toxicity of AGA codons proximal to the start codon or prior to the termination codon in bacteria. The codon sequences of the 'mini-ORFs' employed were initiator, combinations of AGA and CGA, and terminator. Both, AGA and CGA are low-us...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkh784
更新日期:2004-08-18 00:00:00
abstract::An electrophoretic system was established that resolves poly(adenosine diphosphate ribose), enzymatically synthesized polymer from NAD+, by size difference of one residue on polyacrylamide gel. The existence of a polymer of at least 65 residues was demonstrated by band counting in this system. The polymer showed a het...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/5.9.3183
更新日期:1978-09-01 00:00:00