Abstract:
:Over the past 10 years, fluorescent end-labeling of DNA fragments has evolved into the preferred method of DNA detection for a wide variety of applications, including DNA sequencing and PCR fragment analysis. One of the advantages inherent in fluorescent detection methods is the ability to perform multi-color analyses. Unfortunately, labeling DNA fragments with different fluorescent tags generally induces disparate relative electrophoretic mobilities for the fragments. Mobility-shift corrections must therefore be applied to the electrophoretic data to compensate for these effects. These corrections may lead to increased errors in the estimation of DNA fragment sizes and reduced confidence in DNA sequence information. Here, we present a systematic study of the relationship between dye structure and the resultant electrophoretic mobility of end-labeled DNA fragments. We have used a cyanine dye family as a paradigm and high-resolution capillary array electrophoresis (CAE) as the instrumentation platform. Our goals are to develop a general understanding of the effects of dyes on DNA electrophoretic mobility and to synthesize a family of DNA end-labels that impart identically matched mobility influences on DNA fragments. Such matched sets could be used in DNA sequencing and fragment sizing applications on capillary electrophoresis instrumentation.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Tu O,Knott T,Marsh M,Bechtol K,Harris D,Barker D,Bashkin Jdoi
10.1093/nar/26.11.2797subject
Has Abstractpub_date
1998-06-01 00:00:00pages
2797-802issue
11eissn
0305-1048issn
1362-4962pii
gkb450journal_volume
26pub_type
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更新日期:2016-03-18 00:00:00
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journal_title:Nucleic acids research
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更新日期:1984-01-25 00:00:00
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journal_title:Nucleic acids research
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更新日期:2017-10-13 00:00:00
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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更新日期:2006-01-01 00:00:00
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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更新日期:2004-12-15 00:00:00
abstract::Patterns in biological sequences frequently signify interesting features in the underlying molecule. Many tools exist to search for well-known patterns. Less support is available for exploratory analysis, where no well-defined patterns are known yet. PatScanUI (https://patscan.secondarymetabolites.org/) provides a hig...
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更新日期:2018-07-02 00:00:00
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journal_title:Nucleic acids research
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doi:10.1093/nar/27.3.756
更新日期:1999-02-01 00:00:00
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journal_title:Nucleic acids research
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更新日期:1974-03-01 00:00:00
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journal_title:Nucleic acids research
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更新日期:2000-02-01 00:00:00
abstract::Multiple sequence alignments play a central role in the annotation of novel genomes. Given the biological and computational complexity of this task, the automatic generation of high-quality alignments remains challenging. Since multiple alignments are usually employed at the very start of data analysis pipelines, it i...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gki1020
更新日期:2005-12-16 00:00:00