Abstract:
:The gene for the thymidine kinase (TK) of Herpes simplex virus type 1 (HSV-1) is located in the KpnI m and BamHI p fragments of the genome (Wigler et al., Cell 11, 223-232 (1977)). These fragments have been inserted into the EcoRI and BamHI sites, respectively, of plasmid pBR322, and propagated in E.coli. The TK gene contained in the recombinant plasmids was shown to be biologically active when introduced into TK- mouse L cells. Detailed restriction site maps of the BamHI p fragment have been constructed and the approximate location of the TK gene has been determined. Mouse cells transformed with cloned HSV-1 tk+ DNA produced HSV-1-specific thymidine kinase; superinfection with HSV-1 tk- virus increased the level of TK activity tenfold, suggesting that the BamHI p sequences present in transformed cells respond to virus-encoded regulatory gene product(s).
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Wilkie NM,Clements JB,Boll W,Mantei N,Lonsdale D,Weissmann Cdoi
10.1093/nar/7.4.859subject
Has Abstractpub_date
1979-10-25 00:00:00pages
859-77issue
4eissn
0305-1048issn
1362-4962journal_volume
7pub_type
杂志文章abstract::We have purified to near homogeneity a novel nuclear protein from HeLa cells, that specifically binds to scaffold or matrix attachment region DNA elements (S/MAR DNA). The protein, designated SAF-B for scaffold attachment factor B, is an abundant component of chromatin, but not of the nuclear matrix and is expressed i...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/24.5.843
更新日期:1996-03-01 00:00:00
abstract::Lnc2Meth (http://www.bio-bigdata.com/Lnc2Meth/), an interactive resource to identify regulatory relationships between human long non-coding RNAs (lncRNAs) and DNA methylation, is not only a manually curated collection and annotation of experimentally supported lncRNAs-DNA methylation associations but also a platform t...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx985
更新日期:2018-01-04 00:00:00
abstract::The presence of the C.C mispair in a defined duplex oligodeoxynucleotide enhanced its capacity to serve as a substrate for highly purified human DNA methyltransferase. Analysis of tritiated reaction products showed that the C.C mispair acted as a "methylation acceptor" in that it was itself rapidly methylated. The m5C...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/15.17.6899
更新日期:1987-09-11 00:00:00
abstract::In apurinic acid, a single-stranded polydeoxyribonucleotide easily obtained upon depurination of DNA, the proton resonances arising from thymine and cytosine are readily observable in aqueous solution of 25 degrees C. Two methyl thymine resonances, centered at 1.88 ppm and separated by 0.045 ppm, are observed. We attr...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/1.8.999
更新日期:1974-08-01 00:00:00
abstract::The HIV-1 viral infectivity factor (Vif) allows productive infection of non-permissive cells (including most natural HIV-1 targets) by counteracting the cellular cytosine deaminases APOBEC-3G (hA3G) and hA3F. The Vif-induced degradation of these restriction factors by the proteasome has been extensively studied, but l...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp1009
更新日期:2010-01-01 00:00:00
abstract::The methyltransferase, M.EcoKI, recognizes the DNA sequence 5'-AACNNNNNNGTGC-3' and methylates adenine at the underlined positions. DNA methylation has been shown by crystallography to occur via a base flipping mechanism and is believed to be a general mechanism for all methyltransferases. If no structure is available...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkh531
更新日期:2004-04-23 00:00:00
abstract::In this paper we focus on the detection of differentially expressed genes according to changes in hybridization signals using statistical tests. These tests were applied to 14 208 zebrafish cDNA clones that were immobilized on a nylon support and hybridized with radioactively labeled target mRNA from wild-type and lit...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/29.23.e117
更新日期:2001-12-01 00:00:00
abstract::A growing body of evidence suggests that siRNA could generate off-target effects through different mechanisms. However, the full impact of off-target gene regulation on phenotypic induction and accordingly on data interpretation in the context of large-scale siRNA library screen has not been reported. Here we report o...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gki762
更新日期:2005-08-09 00:00:00
abstract::The nuclear receptor vitamin D receptor (VDR) is known to associate with two vitamin D response element (VDRE) containing chromatin regions of the insulin-like growth factor binding protein 3 (IGFBP3) gene. In non-malignant MCF-10A human mammary cells, we show that the natural VDR ligand 1α,25-dihydroxyvitamin D(3) (1...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq820
更新日期:2011-01-01 00:00:00
abstract::Zinc fingers are small protein domains in which zinc plays a structural role contributing to the stability of the domain. Zinc fingers are structurally diverse and are present among proteins that perform a broad range of functions in various cellular processes, such as replication and repair, transcription and transla...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg161
更新日期:2003-01-15 00:00:00
abstract::The modulation of GLI2, an oncogenic transcription factor commonly upregulated in cancer, is in many cases not due to genetic defects, suggesting dysregulation through alternative mechanisms. The identity of these molecular events remains for the most part unknown. Here, we identified TFII-I as a novel repressor of GL...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkaa476
更新日期:2020-07-27 00:00:00
abstract::RsaE is a regulatory RNA highly conserved amongst Firmicutes that lowers the amount of mRNAs associated with the TCA cycle and folate metabolism. A search for new RsaE targets in Staphylococcus aureus revealed that in addition to previously described substrates, RsaE down-regulates several genes associated with argini...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky584
更新日期:2018-09-28 00:00:00
abstract::Long terminal repeat (LTR)-retrotransposons comprise a significant portion of the rice genome. Their complete characterization is thus necessary if the sequenced genome is to be annotated correctly. In addition, because LTR-retrotransposons can influence the expression of neighboring genes, the complete identification...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl780
更新日期:2007-01-01 00:00:00
abstract::The pac1+ gene of the fission yeast Schizosaccharomyces pombe is essential for viability and its overexpression induces sterility and suppresses mutations in the pat1+ and snm1+ genes. The pac1+ gene encodes a protein that is structurally similar to RNase III from Escherichia coli, but its normal function is unknown. ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/24.12.2377
更新日期:1996-06-15 00:00:00
abstract::We describe a cell-free chromatin assembly system derived from the yeast Saccharomyces cerevisiae, which efficiently packages DNA into minichromosomes in a reaction dependent on exogenous core histones and an ATP-regenerating system. Both supercoiled and relaxed plasmid DNA serve as templates for nucleosomal loading i...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gnh107
更新日期:2004-07-28 00:00:00
abstract::DNA bearing lambda operator sequences was methylated by dimethyl sulfate (DMS) in the presence or absence of lambda repressor. Under the experimental conditions, DMS methylates only the purine residues. The presence of lambda repressor affects only the methylation of certain G residues in the operators. Repressor bloc...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/4.5.1595
更新日期:1977-01-01 00:00:00
abstract::The potency of the immune response has still to be harnessed effectively to combat human cancers. However, the discovery of T-cell targets in melanomas and other tumors has raised the possibility that cancer vaccines can be used to induce a therapeutically effective immune response against cancer. The targets, cancer-...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkn673
更新日期:2009-01-01 00:00:00
abstract::Microsatellites, such as (TG)n found at random throughout the genome, or as 3' extensions of Alu sequences are being increasingly used as genetic markers because of their pluriallelic character. The search for polymorphic microsatellites is time consuming, however, as it is necessary to sequence clones containing the ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/20.6.1333
更新日期:1992-03-25 00:00:00
abstract::The distribution patterns of shared short repetitive motifs in the expansion segments of the large subunit rRNA genes of different species show that these segments are coevolving as a set and that in two examined vertebrate species the RNA secondary structures are conserved as a consequence of runs of motifs in one re...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/18.20.5949
更新日期:1990-10-25 00:00:00
abstract::Homologous recombination (HR) is a highly accurate mechanism of DNA repair that can be exploited for homology-directed gene targeting. Since in most cell types HR occurs very infrequently (approximately 10(-6) to 10(-8)), its practical application has been largely restricted to specific experimental systems that allow...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp643
更新日期:2009-09-01 00:00:00
abstract::Impaired DNA damage repair, especially deficient transcription-coupled nucleotide excision repair, leads to segmental progeroid syndromes in human patients as well as in rodent models. Furthermore, DNA double-strand break signalling has been pinpointed as a key inducer of cellular senescence. Several recent findings s...
journal_title:Nucleic acids research
pub_type: 杂志文章,评审
doi:10.1093/nar/gkm1065
更新日期:2007-01-01 00:00:00
abstract::As proteomics initiatives mature, the need will arise for the multiple visualization of proteins and supramolecular complexes within their true context, in situ. Single-stranded DNA and RNA aptamers can be used for low resolution imaging of cellular receptors and cytoplasmic proteins by light microscopy (LM). These te...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gnh164
更新日期:2004-11-23 00:00:00
abstract::In addition to the cap-dependent mechanism, eukaryotic initiation of translation can occur by a cap-independent mechanism which directs ribosomes to defined start codons enabled by internal ribosome entry site (IRES) elements. IRES elements from poliovirus and encephalomyocarditis virus are often used to construct bi-...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/29.16.3327
更新日期:2001-08-15 00:00:00
abstract::A system for the tetracycline-inducible regulation of gene expression in mycobacteria has been developed. We have sub-cloned the tetRO region from the Corynebacterium glutamicum TetZ locus into a mycobacterial shuttle plasmid, making expression of genes cloned downstream of tetRO responsive to tetracycline. Using the ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gni023
更新日期:2005-02-01 00:00:00
abstract::Detection of cancer-associated somatic mutations has broad applications for oncology and precision medicine. However, this becomes challenging when cancer-derived DNA is in low abundance, such as in impure tissue specimens or in circulating cell-free DNA. Next-generation sequencing (NGS) is particularly prone to techn...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkz474
更新日期:2019-09-05 00:00:00
abstract::The 338.5 kb of the Escherichia coli genome described here together with previously described segments bring the total of contiguous finished sequence of this genome to > 1 Mb. Of 319 open reading frames (ORFs) found in this 338.5 kb segment, 147 (46%) are potential new genes. The positions of several genes which had ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/23.12.2105
更新日期:1995-06-25 00:00:00
abstract::Hepatitis B virus (HBV) replication is initiated by HBV RT binding to the highly conserved encapsidation signal, epsilon, at the 5' end of the RNA pregenome. Epsilon contains an apical stem-loop, whose residues are either totally conserved or show rare non-disruptive mutations. Here we present the structure of the api...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl582
更新日期:2006-01-01 00:00:00
abstract::The APOBEC3 proteins are unique to mammals. Many inhibit retrovirus infection through a cDNA cytosine deamination mechanism. HIV-1 neutralizes this host defense through Vif, which triggers APOBEC3 ubiquitination and degradation. Here, we report an APOBEC3F-like, double deaminase domain protein from three artiodactyls:...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl721
更新日期:2006-01-01 00:00:00
abstract::Deep mutational scanning has been used to create high-resolution DNA sequence maps that illustrate the functional consequences of large numbers of point mutations. However, this approach has not yet been applied to libraries of genes created by random circular permutation, an engineering strategy that is used to creat...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky255
更新日期:2018-07-27 00:00:00
abstract::Proline-rich protein (PRP) gene MP6 was isolated from a mouse BALB/c genomic DNA library in lambda EMBL3, characterised by hybridisation and restriction mapping and the promoter region, from -162 to +72 around the PRP consensus cap-site, was sequenced. In gel shift assays this region formed complexes C1 and C2 with pa...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/19.19.5205
更新日期:1991-10-11 00:00:00