Abstract：
:The distribution patterns of shared short repetitive motifs in the expansion segments of the large subunit rRNA genes of different species show that these segments are coevolving as a set and that in two examined vertebrate species the RNA secondary structures are conserved as a consequence of runs of motifs in one region being compensated by complementary motifs in another. These unusual processes, involving replication-slippage, have implications for the evolution of ribosomal RNA and for the use of the rDNA multigene family as a 'molecular clock' for assessing relationships between species.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Hancock JM,Dover GAdoi
10.1093/nar/18.20.5949subject
Has Abstractpub_date
1990-10-25 00:00:00pages
5949-54issue
20eissn
0305-1048issn
1362-4962journal_volume
18pub_type
杂志文章abstract：:The hybridization of complementary strands of DNA is the underlying principle of all microarray-based techniques for the analysis of DNA variation. In this paper, we study how probe immobilization at surfaces, specifically probe density, influences the kinetics of target capture using surface plasmon resonance (SPR) s...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/29.24.5163
更新日期：2001-12-15 00:00:00
abstract：:The process of strand exchange is considered to be the hallmark of DNA recombination. Proteins known to carry out such exchange are believed to operate via one or the other of two mechanisms. RecA-like proteins promote the formation of a three-stranded or triplex synaptic intermediate in which strand exchange occurs, ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/25.20.4004
更新日期：1997-10-15 00:00:00
abstract：:Upstream open reading frame (uORF)-mediated translational inhibition is important in controlling key regulatory genes expression. However, understanding the underlying molecular mechanism of such uORF-mediated control system in vivo is challenging in the absence of an animal model. Therefore, we generated a zebrafish ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkr645
更新日期：2011-11-01 00:00:00
abstract：:The accurate structural modeling of B- and T-cell receptors is fundamental to gain a detailed insight in the mechanisms underlying immunity and in developing new drugs and therapies. The LYRA (LYmphocyte Receptor Automated modeling) web server (http://www.cbs.dtu.dk/services/LYRA/) implements a complete and automated ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkv535
更新日期：2015-07-01 00:00:00
abstract：:Chicken-erythrocyte inner histone tetramer has been complexed with several natural and synthetic DNA duplexes by salt-gradient dialysis at various protein/DNA ratios. The resulting complexes, in low-ionic-strength buffer, have been examined by electron microscopy, circular dichroism, and thermal denaturation. Electron...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/5.10.3603
更新日期：1978-10-01 00:00:00
abstract：:Non-coding genetic variants/mutations can play functional roles in the cell by disrupting regulatory interactions between transcription factors (TFs) and their genomic target sites. For most human TFs, a myriad of DNA-binding models are available and could be used to predict the effects of DNA mutations on TF binding....
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz363
更新日期：2019-07-02 00:00:00
abstract：:The thermal denaturation spectra of E. coli 50s and 30s ribosomal sub units and of their isolated RNA's were studied over the wavelength range of 2300-3000 Angstrom. It was possible to fit the experimental denaturation spectra with super positions of the reference spectra for denaturation of A - U and G - C base pairs...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/2.3.373
更新日期：1975-03-01 00:00:00
abstract：:We have developed a novel multiplex quantitative DNA-based target amplification method suitable for sensitive, specific and quantitative detection on microarray. This new method named NASBA Implemented Microarray Analysis (NAIMA) was applied to GMO detection in food and feed, but its application can be extended to all...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn524
更新日期：2008-10-01 00:00:00
abstract：:Labeling of a covalently closed circular double-stranded DNA was achieved using a so-called 'padlock oligonucleotide'. The oligonucleotide was targeted to a sequence which is present in the replication origin of phage f1 and thus in numerous commonly used plasmids. After winding around the double-stranded target DNA s...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/30.3.e12
更新日期：2002-02-01 00:00:00
abstract：:From a recombinant lambda phage, we have determined a 387 bp sequence containing a mouse lysine tRNA gene. The putative lys tRNA (anticodon UUU) differs from rabbit liver lys tRNA at five positions. The flanking regions of the mouse gene are not generally homologous to published human and Drosophila lys tRNA genes. Ho...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/11.7.2053
更新日期：1983-04-11 00:00:00
abstract：:Xenopus laevis 5S rRNA isolated from 7S particles or transcribed in vitro is found to adopt two alternative conformations. These two conformers contain different structural elements within the major TF III A binding domain, which, when isolated as RNA fragments, still interact with the transcription factor. Chemical m...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/14.15.6313
更新日期：1986-08-11 00:00:00
abstract：:The temperature-sensitive Neurospora nuclear mutant cyt18-1 is deficient in splicing many Group I mitochondrial introns when grown at its non-permissive temperature; however, splicing of intron 1 in the coI gene of the Adiopodoume (formerly called North Africa) strain is unaffected (R.A. Collins and A.M. Lambowitz, J....
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/17.22.9087
更新日期：1989-11-25 00:00:00
abstract：:Serine integrases are emerging as core tools in synthetic biology and have applications in biotechnology and genome engineering. We have designed a split-intein serine integrase-based system with potential for regulation of site-specific recombination events at the protein level in vivo. The ϕC31 integrase was split i...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz936
更新日期：2019-12-02 00:00:00
abstract：:Variable (diversity) joining [V(D)J] recombination of immune gene loci proceeds in an ordered manner with D to J portions recombining first and then an upstream V joins that recombinant. We present evidence that the non-core domain of recombination activating gene (RAG) protein 2 is involved in the regulation of recom...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn553
更新日期：2008-10-01 00:00:00
abstract：:In order to systematically analyze the effects of nucleoside modification of sugar moieties in DNA polymerase reactions, we synthesized 16 modified templates containing 2',4'-bridged nucleotides and three types of 2',4'-bridged nucleoside-5'-triphospates with different bridging structures. Among the five types of ther...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn404
更新日期：2008-08-01 00:00:00
abstract：:Distamycin binds the minor groove of duplex DNA at AT-rich regions and has been a valuable probe of protein interactions with double-stranded DNA. We find that distamycin can also inhibit protein interactions with G-quadruplex (G4) DNA, a stable four-stranded structure in which the repeating unit is a G-quartet. Using...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkg392
更新日期：2003-06-01 00:00:00
abstract：:Plasmid DNA incubated in interphase Xenopus egg extracts is normally assembled into chromatin and then into synthetic nuclei which undergo one round of regulated replication. During a study of restriction endonuclease cut plasmid replication intermediates (RIs) by the Brewer-Fangman 2D gel electrophoresis technique, w...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/28.10.2187
更新日期：2000-05-15 00:00:00
abstract：:Reverse gyrase is a peculiar DNA topoisomerase, specific of hyperthermophilic Archaea and Bacteria, which has the unique ability of introducing positive supercoiling into DNA molecules. Although the function of the enzyme has not been established directly, it has been suggested to be involved in DNA protection and rep...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkl115
更新日期：2006-04-14 00:00:00
abstract：:Synchronized cells of a normal human lymphocytic cell line contain little swiven enzyme activity in G0 and G1 and high activity in Sphase. The level of activity in different growth phase appears to be related to the fraction of the population engaged in DNA replication. No endogenous inhibitor or activator of swiven a...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/3.12.3305
更新日期：1976-12-01 00:00:00
abstract：:Equilibrium and kinetics of thermal melting of yeast 5S ribosomal RNA in aqueous NaCl with or without Mg2+ were investigated by differential thermal melting and temperature jump methods. Two peaks (1 and 2) and a shoulder were observed in each of melting curves at ionic strength I=0.002-0.5 and linearity between each ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/11.10.3363
更新日期：1983-05-25 00:00:00
abstract：:Synthetic oligonucleotides were used to introduce mutations into the lacPUV5 promoter. Four mutations were obtained at positions -13, -14, and -15, with respect to the transcriptional start site. The effects of these mutations were measured in vivo and the results are discussed with respect to the consensus sequence a...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/12.9.4011
更新日期：1984-05-11 00:00:00
abstract：:We have recently shown that phosphorothioate (PS) oligodeoxynucleotide (ODN) analogs, unlike their normal congeners, exhibit significant anti-HIV activity (Matsukura et al., (1987) Proc. Natl. Acad. Sci. USA 84, 7706-7710). We now report the syntheses, melting temperatures (Tm), and nuclease susceptibilities of a seri...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/16.8.3209
更新日期：1988-04-25 00:00:00
abstract：:Small interfering RNAs (siRNAs) are promising new active compounds in gene medicine but the induction of non-specific immune responses following their delivery continues to be a serious problem. With the purpose of avoiding such effects chemically modified siRNAs are tested in screening assay but often only examining ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks033
更新日期：2012-05-01 00:00:00
abstract：:Motor proteins that translocate on nucleic acids are key players in gene expression and maintenance. While the function of these proteins is diverse, they are driven by highly conserved core motor domains. In transcription-coupled DNA repair, motor activity serves to remove RNA polymerase stalled on damaged DNA, makin...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp680
更新日期：2009-10-01 00:00:00
abstract：:We have previously identified a T lymphocyte protein which binds to a site within the LTR of the human immunodeficiency virus type 1 (HIV-1) and exerts an inhibitory effect on virus gene expression. The palindromic site (site B) recognized by this protein is related to the palindromic binding sites of members of the s...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/20.20.5429
更新日期：1992-10-25 00:00:00
abstract：:We have developed a new method for analysis of complex genetic systems by a combination of the Amplification Refractory Mutation System (ARMS) and Single-Strand Conformation Polymorphism (SSCP) analysis: ARMS-SSCP. Thus, a complex allelic series is subdivided into a number of groups by ARMS followed by the identificat...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/20.5.1005
更新日期：1992-03-11 00:00:00
abstract：:Many multimeric transcription factors recognize DNA sequence patterns by cooperatively binding to bipartite elements composed of half sites separated by a flexible spacer. We developed a novel bipartite algorithm, bipartite pattern discovery (Bipad), which produces a mathematical model based on information maximizatio...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkh825
更新日期：2004-09-23 00:00:00
abstract：:Immature oocytes from Xenopus laevis contain a 42S ribonucleoprotein particle (RNP) containing 5S RNA, tRNA, a 43 kDa protein, and a 48 kDa protein. A particle containing 5S RNA and the 43 kDa protein (p43-5S) liberated from the 42S particle upon brief treatment with urea can be purified by anion exchange chromatograp...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/19.8.1797
更新日期：1991-04-25 00:00:00
abstract：:Planarian flatworms are in the midst of a renaissance as a model system for regeneration and stem cells. Besides two well-studied model species, hundreds of species exist worldwide that present a fascinating diversity of regenerative abilities, tissue turnover rates, reproductive strategies and other life history trai...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkv1148
更新日期：2016-01-04 00:00:00
abstract：:We previously reported the isolation of PRP28, a gene in Saccharomyces cerevisiae whose activity is required for the first step of nuclear mRNA splicing in vivo. Sequence analysis revealed that PRP28 is included in the 'DEAD-box' gene family, members of which are thought to function as ATP-dependent RNA helicases. Gen...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/22.15.3187
更新日期：1994-08-11 00:00:00
